UniProtKB - P61830 (H3_YEAST)
Histone H3
HHT1
moreFunctioni
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Component of the UAF (upstream activation factor) complex which interacts with the upstream element of the RNA polymerase I promoter and forms a stable preinitiation complex. Together with SPT15/TBP, UAF seems to stimulate basal transcription to a fully activated level.
Miscellaneous
Caution
GO - Molecular functioni
- DNA binding Source: SGD
- protein heterodimerization activity Source: InterPro
GO - Biological processi
- chromatin assembly or disassembly Source: SGD
- global genome nucleotide-excision repair Source: SGD
- nucleolar large rRNA transcription by RNA polymerase I Source: ComplexPortal
- positive regulation of transcription by RNA polymerase I Source: ComplexPortal
- regulation of transcription, DNA-templated Source: ComplexPortal
- rRNA transcription Source: SGD
- sexual sporulation resulting in formation of a cellular spore Source: SGD
Keywordsi
Molecular function | DNA-binding |
Enzyme and pathway databases
Reactomei | R-SCE-2299718, Condensation of Prophase Chromosomes R-SCE-2559580, Oxidative Stress Induced Senescence R-SCE-427359, SIRT1 negatively regulates rRNA expression R-SCE-5625886, Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 R-SCE-68616, Assembly of the ORC complex at the origin of replication R-SCE-73772, RNA Polymerase I Promoter Escape R-SCE-9018519, Estrogen-dependent gene expression |
SABIO-RKi | P61830 |
Names & Taxonomyi
Protein namesi | Recommended name: Histone H3 |
Gene namesi | Name:HHT1 Ordered Locus Names:YBR010W ORF Names:YBR0201 AND Name:HHT2 Synonyms:SIN2 Ordered Locus Names:YNL031C ORF Names:N2749 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000214, HHT1 S000004976, HHT2 |
VEuPathDBi | FungiDB:YBR010W FungiDB:YNL031C |
Subcellular locationi
Nucleus
Other locations
Nucleus
- nucleus Source: SGD
- replication fork protection complex Source: SGD
- RNA polymerase I upstream activating factor complex Source: ComplexPortal
Other locations
- CENP-A containing nucleosome Source: SGD
- nucleosome Source: ComplexPortal
Keywords - Cellular componenti
Chromosome, Nucleosome core, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 11 | S → A: Impairs histone H3 phosphorylation and reduces transcription of some GCN5 regulated genes. 2 Publications | 1 | |
Mutagenesisi | 53 | R → A, K or Q: Lethal. 1 Publication | 1 | |
Mutagenesisi | 57 | K → A, Q or R: Increases sensitivity to genotoxic agents inducing DNA breaks during replication. 3 Publications | 1 | |
Mutagenesisi | 80 | K → A, P or Q: Compromises telomeric silencing. 2 Publications | 1 | |
Mutagenesisi | 119 | T → A or E: Lethal. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000221370 | 2 – 136 | Histone H3Add BLAST | 135 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 5 | N6,N6,N6-trimethyllysine; alternate11 Publications | 1 | |
Modified residuei | 5 | N6,N6-dimethyllysine; alternate11 Publications | 1 | |
Modified residuei | 5 | N6-methyllysine; alternate11 Publications | 1 | |
Modified residuei | 10 | N6-acetyllysine; alternate5 Publications | 1 | |
Modified residuei | 10 | N6-crotonyllysine; alternate1 Publication | 1 | |
Modified residuei | 10 | N6-methyllysine; alternate1 Publication | 1 | |
Modified residuei | 11 | Phosphoserine3 Publications | 1 | |
Modified residuei | 15 | N6,N6-dimethyllysine; alternate1 Publication | 1 | |
Modified residuei | 15 | N6-acetyllysine; alternate8 Publications | 1 | |
Modified residuei | 15 | N6-butyryllysine; alternate2 Publications | 1 | |
Modified residuei | 19 | N6-acetyllysine; alternate5 Publications | 1 | |
Modified residuei | 19 | N6-butyryllysine; alternate1 Publication | 1 | |
Modified residuei | 19 | N6-methyllysine; alternate1 Publication | 1 | |
Modified residuei | 24 | N6-acetyllysine; alternate3 Publications | 1 | |
Modified residuei | 24 | N6-butyryllysine; alternate1 Publication | 1 | |
Modified residuei | 24 | N6-methyllysine; alternate1 Publication | 1 | |
Modified residuei | 24 | N6-propionyllysine; alternate1 Publication | 1 | |
Modified residuei | 28 | N6,N6,N6-trimethyllysine; alternate1 Publication | 1 | |
Modified residuei | 28 | N6,N6-dimethyllysine; alternate1 Publication | 1 | |
Modified residuei | 28 | N6-acetyllysine; alternate3 Publications | 1 | |
Modified residuei | 28 | N6-butyryllysine; alternate1 Publication | 1 | |
Modified residuei | 28 | N6-methyllysine; alternate1 Publication | 1 | |
Modified residuei | 37 | N6,N6,N6-trimethyllysine; alternate7 Publications | 1 | |
Modified residuei | 37 | N6,N6-dimethyllysine; alternate8 Publications | 1 | |
Modified residuei | 37 | N6-acetyllysine; alternate3 Publications | 1 | |
Modified residuei | 37 | N6-methyllysine; alternate8 Publications | 1 | |
Modified residuei | 38 | N6-acetyllysine; alternate1 Publication | 1 | |
Modified residuei | 38 | N6-methyllysine; alternate1 Publication | 1 | |
Modified residuei | 57 | N6-acetyllysine6 Publications | 1 | |
Modified residuei | 57 | N6-malonyllysine; alternate1 Publication | 1 | |
Modified residuei | 57 | N6-propionyllysine; alternate1 Publication | 1 | |
Modified residuei | 65 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 80 | N6,N6,N6-trimethyllysine; alternate6 Publications | 1 | |
Modified residuei | 80 | N6,N6-dimethyllysine; alternate6 Publications | 1 | |
Modified residuei | 80 | N6-methyllysine; alternate6 Publications | 1 | |
Modified residuei | 80 | N6-succinyllysine; alternate1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
MaxQBi | P61830 |
PaxDbi | P61830 |
PRIDEi | P61830 |
PTM databases
CarbonylDBi | P61830 |
iPTMneti | P61830 |
Interactioni
Subunit structurei
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Histone H3 is a component of the UAF (upstream activation factor) complex, which consists of UAF30, RRN5, RRN9, RRN10, and histones H3 and H4.
1 PublicationBinary interactionsi
P61830
GO - Molecular functioni
- protein heterodimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 32711, 859 interactors 35796, 703 interactors |
ComplexPortali | CPX-1101, RNA polymerase I upstream activating factor complex CPX-1610, Nucleosome, variant HTA2-HTB2 CPX-1611, Nucleosome, variant HTA2-HTB1 CPX-1612, Nucleosome, variant HTA1-HTB1 CPX-1613, Nucleosome, variant HTZ1-HTB1 CPX-1614, Nucleosome, variant HTZ1-HTB2 CPX-2566, Nucleosome, variant HTA1-HTB2 |
DIPi | DIP-417N |
IntActi | P61830, 153 interactors |
MINTi | P61830 |
STRINGi | 4932.YBR010W |
Miscellaneous databases
RNActi | P61830, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P61830 |
SMRi | P61830 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P61830 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 43 | DisorderedSequence analysisAdd BLAST | 43 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1745, Eukaryota |
GeneTreei | ENSGT01040000240398 |
HOGENOMi | CLU_078295_4_0_1 |
InParanoidi | P61830 |
OMAi | HIVMART |
Family and domain databases
Gene3Di | 1.10.20.10, 1 hit |
IDEALi | IID50143 |
InterProi | View protein in InterPro IPR009072, Histone-fold IPR007125, Histone_H2A/H2B/H3 IPR000164, Histone_H3/CENP-A |
PANTHERi | PTHR11426, PTHR11426, 1 hit |
Pfami | View protein in Pfam PF00125, Histone, 1 hit |
PRINTSi | PR00622, HISTONEH3 |
SMARTi | View protein in SMART SM00428, H3, 1 hit |
SUPFAMi | SSF47113, SSF47113, 1 hit |
PROSITEi | View protein in PROSITE PS00322, HISTONE_H3_1, 1 hit PS00959, HISTONE_H3_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MARTKQTARK STGGKAPRKQ LASKAARKSA PSTGGVKKPH RYKPGTVALR
60 70 80 90 100
EIRRFQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSSAI GALQESVEAY
110 120 130
LVSLFEDTNL AAIHAKRVTI QKKDIKLARR LRGERS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 124 | D → E AA sequence (PubMed:7035169).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00724 Genomic DNA Translation: CAA25310.1 X00725 Genomic DNA Translation: CAA25312.1 Z35879 Genomic DNA Translation: CAA84948.1 Z71306 Genomic DNA Translation: CAA95893.1 Z71307 Genomic DNA Translation: CAA95894.1 AY558343 Genomic DNA Translation: AAS56669.1 AY692987 Genomic DNA Translation: AAT93006.1 AY554000 Genomic DNA Translation: AAS64341.1 AY554001 Genomic DNA Translation: AAS64342.1 AY554002 Genomic DNA Translation: AAS64343.1 AY554003 Genomic DNA Translation: AAS64344.1 AY554004 Genomic DNA Translation: AAS64345.1 AY554005 Genomic DNA Translation: AAS64346.1 AY554006 Genomic DNA Translation: AAS64347.1 AY554007 Genomic DNA Translation: AAS64348.1 AY554008 Genomic DNA Translation: AAS64349.1 BK006936 Genomic DNA Translation: DAA07131.1 BK006947 Genomic DNA Translation: DAA10514.1 |
PIRi | S45265, HSBY3 |
RefSeqi | NP_009564.1, NM_001178358.1 NP_014367.1, NM_001182870.1 |
Genome annotation databases
EnsemblFungii | YBR010W_mRNA; YBR010W; YBR010W YNL031C_mRNA; YNL031C; YNL031C |
GeneIDi | 852295 855700 |
KEGGi | sce:YBR010W sce:YNL031C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00724 Genomic DNA Translation: CAA25310.1 X00725 Genomic DNA Translation: CAA25312.1 Z35879 Genomic DNA Translation: CAA84948.1 Z71306 Genomic DNA Translation: CAA95893.1 Z71307 Genomic DNA Translation: CAA95894.1 AY558343 Genomic DNA Translation: AAS56669.1 AY692987 Genomic DNA Translation: AAT93006.1 AY554000 Genomic DNA Translation: AAS64341.1 AY554001 Genomic DNA Translation: AAS64342.1 AY554002 Genomic DNA Translation: AAS64343.1 AY554003 Genomic DNA Translation: AAS64344.1 AY554004 Genomic DNA Translation: AAS64345.1 AY554005 Genomic DNA Translation: AAS64346.1 AY554006 Genomic DNA Translation: AAS64347.1 AY554007 Genomic DNA Translation: AAS64348.1 AY554008 Genomic DNA Translation: AAS64349.1 BK006936 Genomic DNA Translation: DAA07131.1 BK006947 Genomic DNA Translation: DAA10514.1 |
PIRi | S45265, HSBY3 |
RefSeqi | NP_009564.1, NM_001178358.1 NP_014367.1, NM_001182870.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ID3 | X-ray | 3.10 | A/E | 2-136 | [»] | |
1M1D | X-ray | 2.20 | B/D | 2-21 | [»] | |
1PEG | X-ray | 2.59 | P/Q | 2-16 | [»] | |
1PU9 | X-ray | 2.30 | B | 6-24 | [»] | |
1PUA | X-ray | 2.30 | B | 6-24 | [»] | |
1QSN | X-ray | 2.20 | B | 10-20 | [»] | |
2CNX | X-ray | 2.10 | P | 2-6 | [»] | |
2H2G | X-ray | 1.63 | B | 114-124 | [»] | |
2IDC | X-ray | 2.20 | A | 119-135 | [»] | |
2JMJ | NMR | - | P | 2-10 | [»] | |
2RNW | NMR | - | B | 2-16 | [»] | |
2RNX | NMR | - | B | 32-43 | [»] | |
2RSN | NMR | - | B | 2-18 | [»] | |
3MP1 | X-ray | 2.60 | P | 2-6 | [»] | |
3MP6 | X-ray | 1.48 | P | 2-5 | [»] | |
3Q33 | X-ray | 2.80 | D | 2-15 | [»] | |
4JJN | X-ray | 3.09 | A/E | 2-136 | [»] | |
4KUD | X-ray | 3.20 | A/E | 1-136 | [»] | |
4PSX | X-ray | 2.51 | P/Y | 2-16 | [»] | |
5D7E | X-ray | 1.90 | C | 6-12 | [»] | |
5IOK | X-ray | 2.22 | C | 6-12 | [»] | |
5ZBA | X-ray | 3.50 | C | 1-136 | [»] | |
5ZBB | X-ray | 3.60 | C | 1-136 | [»] | |
6GEJ | electron microscopy | 3.60 | A/B | 1-136 | [»] | |
6GEN | electron microscopy | 3.60 | A/B | 1-136 | [»] | |
6J2P | X-ray | 2.85 | E/F/G/H | 2-8 | [»] | |
6KMJ | X-ray | 1.40 | C | 7-22 | [»] | |
7K7G | electron microscopy | 4.20 | A/E | 1-136 | [»] | |
AlphaFoldDBi | P61830 | |||||
SMRi | P61830 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32711, 859 interactors 35796, 703 interactors |
ComplexPortali | CPX-1101, RNA polymerase I upstream activating factor complex CPX-1610, Nucleosome, variant HTA2-HTB2 CPX-1611, Nucleosome, variant HTA2-HTB1 CPX-1612, Nucleosome, variant HTA1-HTB1 CPX-1613, Nucleosome, variant HTZ1-HTB1 CPX-1614, Nucleosome, variant HTZ1-HTB2 CPX-2566, Nucleosome, variant HTA1-HTB2 |
DIPi | DIP-417N |
IntActi | P61830, 153 interactors |
MINTi | P61830 |
STRINGi | 4932.YBR010W |
PTM databases
CarbonylDBi | P61830 |
iPTMneti | P61830 |
Proteomic databases
MaxQBi | P61830 |
PaxDbi | P61830 |
PRIDEi | P61830 |
Genome annotation databases
EnsemblFungii | YBR010W_mRNA; YBR010W; YBR010W YNL031C_mRNA; YNL031C; YNL031C |
GeneIDi | 852295 855700 |
KEGGi | sce:YBR010W sce:YNL031C |
Organism-specific databases
SGDi | S000000214, HHT1 S000004976, HHT2 |
VEuPathDBi | FungiDB:YBR010W FungiDB:YNL031C |
Phylogenomic databases
eggNOGi | KOG1745, Eukaryota |
GeneTreei | ENSGT01040000240398 |
HOGENOMi | CLU_078295_4_0_1 |
InParanoidi | P61830 |
OMAi | HIVMART |
Enzyme and pathway databases
Reactomei | R-SCE-2299718, Condensation of Prophase Chromosomes R-SCE-2559580, Oxidative Stress Induced Senescence R-SCE-427359, SIRT1 negatively regulates rRNA expression R-SCE-5625886, Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 R-SCE-68616, Assembly of the ORC complex at the origin of replication R-SCE-73772, RNA Polymerase I Promoter Escape R-SCE-9018519, Estrogen-dependent gene expression |
SABIO-RKi | P61830 |
Miscellaneous databases
EvolutionaryTracei | P61830 |
PROi | PR:P61830 |
RNActi | P61830, protein |
Family and domain databases
Gene3Di | 1.10.20.10, 1 hit |
IDEALi | IID50143 |
InterProi | View protein in InterPro IPR009072, Histone-fold IPR007125, Histone_H2A/H2B/H3 IPR000164, Histone_H3/CENP-A |
PANTHERi | PTHR11426, PTHR11426, 1 hit |
Pfami | View protein in Pfam PF00125, Histone, 1 hit |
PRINTSi | PR00622, HISTONEH3 |
SMARTi | View protein in SMART SM00428, H3, 1 hit |
SUPFAMi | SSF47113, SSF47113, 1 hit |
PROSITEi | View protein in PROSITE PS00322, HISTONE_H3_1, 1 hit PS00959, HISTONE_H3_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | H3_YEAST | |
Accessioni | P61830Primary (citable) accession number: P61830 Secondary accession number(s): D6VQ11 Q6Q7G9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 183 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names - Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families