UniProtKB - P61714 (RISB_ECOLI)
Protein
6,7-dimethyl-8-ribityllumazine synthase
Gene
ribE
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.1 Publication
Catalytic activityi
- (2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H+ + 2 H2O + phosphate1 PublicationEC:2.5.1.781 Publication
Kineticsi
- KM=4.2 µM for 5-amino-6-(D-ribitylamino)uracil1 Publication
- KM=62 µM for 3,4-dihydroxy-2-butanone 4-phosphate1 Publication
- Vmax=11800 nmol/h/mg enzyme1 Publication
: riboflavin biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil.1 PublicationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- 6,7-dimethyl-8-ribityllumazine synthase (ribE), 6,7-dimethyl-8-ribityllumazine synthase (ribE)
- Riboflavin synthase (ribE), Riboflavin synthase (ribC)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 22 | 5-amino-6-(D-ribitylamino)uracilBy similarity | 1 | |
Active sitei | 89 | Proton donorSequence analysis | 1 | |
Binding sitei | 114 | 5-amino-6-(D-ribitylamino)uracil; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 128 | 1-deoxy-L-glycero-tetrulose 4-phosphateBy similarity | 1 |
GO - Molecular functioni
- 6,7-dimethyl-8-ribityllumazine synthase activity Source: EcoCyc
GO - Biological processi
- riboflavin biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Riboflavin biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:LUMAZINESYN-MONOMER MetaCyc:LUMAZINESYN-MONOMER |
SABIO-RKi | P61714 |
UniPathwayi | UPA00275;UER00404 |
Names & Taxonomyi
Protein namesi | Recommended name: 6,7-dimethyl-8-ribityllumazine synthase (EC:2.5.1.78)Short name: DMRL synthase Short name: LS Short name: Lumazine synthase |
Gene namesi | Name:ribE Synonyms:ribH, ybaF Ordered Locus Names:b0415, JW0405 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytosol
- cytosol Source: EcoCyc
Other locations
- riboflavin synthase complex Source: InterPro
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000134754 | 1 – 156 | 6,7-dimethyl-8-ribityllumazine synthaseAdd BLAST | 156 |
Proteomic databases
jPOSTi | P61714 |
PaxDbi | P61714 |
PRIDEi | P61714 |
2D gel databases
SWISS-2DPAGEi | P61714 |
Interactioni
Subunit structurei
Forms a hollow icosahedral capsid composed of 60 subunits, probably arranged as a dodecamer of pentamers. Unlike in B.subtilis, does not interact with riboflavin synthase, and the core of the icosahedral capsid is empty.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4259505, 92 interactors |
DIPi | DIP-10711N |
IntActi | P61714, 16 interactors |
STRINGi | 511145.b0415 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 57 – 59 | 5-amino-6-(D-ribitylamino)uracil bindingBy similarity | 3 | |
Regioni | 81 – 83 | 5-amino-6-(D-ribitylamino)uracil bindingBy similarity | 3 | |
Regioni | 86 – 87 | 1-deoxy-L-glycero-tetrulose 4-phosphate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the DMRL synthase family.Curated
Phylogenomic databases
eggNOGi | COG0054, Bacteria |
HOGENOMi | CLU_089358_1_1_6 |
InParanoidi | P61714 |
PhylomeDBi | P61714 |
Family and domain databases
CDDi | cd09209, Lumazine_synthase-I, 1 hit |
Gene3Di | 3.40.50.960, 1 hit |
HAMAPi | MF_00178, Lumazine_synth, 1 hit |
InterProi | View protein in InterPro IPR034964, LS IPR002180, LS/RS IPR036467, LS/RS_sf |
PANTHERi | PTHR21058, PTHR21058, 1 hit |
Pfami | View protein in Pfam PF00885, DMRL_synthase, 1 hit |
SUPFAMi | SSF52121, SSF52121, 1 hit |
TIGRFAMsi | TIGR00114, lumazine-synth, 1 hit |
i Sequence
Sequence statusi: Complete.
P61714-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNIIEANVAT PDARVAITIA RFNNFINDSL LEGAIDALKR IGQVKDENIT
60 70 80 90 100
VVWVPGAYEL PLAAGALAKT GKYDAVIALG TVIRGGTAHF EYVAGGASNG
110 120 130 140 150
LAHVAQDSEI PVAFGVLTTE SIEQAIERAG TKAGNKGAEA ALTALEMINV
LKAIKA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64395 Genomic DNA Translation: CAA45736.1 U82664 Genomic DNA Translation: AAB40171.1 U00096 Genomic DNA Translation: AAC73518.1 AP009048 Genomic DNA Translation: BAE76195.1 |
PIRi | S26202 |
RefSeqi | NP_414949.1, NC_000913.3 WP_001021161.1, NZ_STEB01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAC73518; AAC73518; b0415 BAE76195; BAE76195; BAE76195 |
GeneIDi | 58460342 946453 |
KEGGi | ecj:JW0405 eco:b0415 |
PATRICi | fig|1411691.4.peg.1862 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64395 Genomic DNA Translation: CAA45736.1 U82664 Genomic DNA Translation: AAB40171.1 U00096 Genomic DNA Translation: AAC73518.1 AP009048 Genomic DNA Translation: BAE76195.1 |
PIRi | S26202 |
RefSeqi | NP_414949.1, NC_000913.3 WP_001021161.1, NZ_STEB01000007.1 |
3D structure databases
SMRi | P61714 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259505, 92 interactors |
DIPi | DIP-10711N |
IntActi | P61714, 16 interactors |
STRINGi | 511145.b0415 |
2D gel databases
SWISS-2DPAGEi | P61714 |
Proteomic databases
jPOSTi | P61714 |
PaxDbi | P61714 |
PRIDEi | P61714 |
Genome annotation databases
EnsemblBacteriai | AAC73518; AAC73518; b0415 BAE76195; BAE76195; BAE76195 |
GeneIDi | 58460342 946453 |
KEGGi | ecj:JW0405 eco:b0415 |
PATRICi | fig|1411691.4.peg.1862 |
Organism-specific databases
EchoBASEi | EB1298 |
Phylogenomic databases
eggNOGi | COG0054, Bacteria |
HOGENOMi | CLU_089358_1_1_6 |
InParanoidi | P61714 |
PhylomeDBi | P61714 |
Enzyme and pathway databases
UniPathwayi | UPA00275;UER00404 |
BioCyci | EcoCyc:LUMAZINESYN-MONOMER MetaCyc:LUMAZINESYN-MONOMER |
SABIO-RKi | P61714 |
Miscellaneous databases
PROi | PR:P61714 |
Family and domain databases
CDDi | cd09209, Lumazine_synthase-I, 1 hit |
Gene3Di | 3.40.50.960, 1 hit |
HAMAPi | MF_00178, Lumazine_synth, 1 hit |
InterProi | View protein in InterPro IPR034964, LS IPR002180, LS/RS IPR036467, LS/RS_sf |
PANTHERi | PTHR21058, PTHR21058, 1 hit |
Pfami | View protein in Pfam PF00885, DMRL_synthase, 1 hit |
SUPFAMi | SSF52121, SSF52121, 1 hit |
TIGRFAMsi | TIGR00114, lumazine-synth, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RISB_ECOLI | |
Accessioni | P61714Primary (citable) accession number: P61714 Secondary accession number(s): P25540, P77114, Q2MC11 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2004 |
Last sequence update: | June 7, 2004 | |
Last modified: | February 10, 2021 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families