UniProtKB - P61458 (PHS_MOUSE)
Pterin-4-alpha-carbinolamine dehydratase
Pcbd1
Functioni
Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcription. Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity (By similarity).
Also acts as a coactivator for HNF1B-dependent transcription (By similarity).
By similarityCatalytic activityi
- (4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2OBy similarityEC:4.2.1.96By similarity
GO - Molecular functioni
- 4-alpha-hydroxytetrahydrobiopterin dehydratase activity Source: MGI
- identical protein binding Source: MGI
- phenylalanine 4-monooxygenase activity Source: MGI
- transcription coactivator activity Source: MGI
GO - Biological processi
- L-phenylalanine metabolic process Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- regulation of protein binding Source: MGI
- tetrahydrobiopterin biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Activator, Lyase |
Biological process | Tetrahydrobiopterin biosynthesis, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-8964208, Phenylalanine metabolism |
Names & Taxonomyi
Protein namesi | Recommended name: Pterin-4-alpha-carbinolamine dehydratase (EC:4.2.1.96By similarity)Short name: PHS Alternative name(s): 4-alpha-hydroxy-tetrahydropterin dehydratase Dimerization cofactor of hepatocyte nuclear factor 1-alpha Short name: DCoH Short name: Dimerization cofactor of HNF1 Phenylalanine hydroxylase-stimulating protein Pterin carbinolamine dehydratase Short name: PCD |
Gene namesi | Name:Pcbd1 Synonyms:Dcoh, Pcbd |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:94873, Pcbd1 |
VEuPathDBi | HostDB:ENSMUSG00000020098 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000063053 | 2 – 104 | Pterin-4-alpha-carbinolamine dehydrataseAdd BLAST | 103 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | P61458 |
MaxQBi | P61458 |
PaxDbi | P61458 |
PeptideAtlasi | P61458 |
PRIDEi | P61458 |
ProteomicsDBi | 288146 |
PTM databases
iPTMneti | P61458 |
PhosphoSitePlusi | P61458 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000020098, Expressed in right kidney and 249 other tissues |
Genevisiblei | P61458, MM |
Interactioni
Subunit structurei
Homotetramer and homodimer. Heterotetramer with HNF1A; formed by a dimer of dimers (By similarity).
Interacts with HNF1B (via HNF-p1 domain); the interaction increases HNF1B transactivation activity (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199068, 21 interactors |
IntActi | P61458, 17 interactors |
MINTi | P61458 |
STRINGi | 10090.ENSMUSP00000020298 |
Miscellaneous databases
RNActi | P61458, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 61 – 63 | Substrate bindingBy similarity | 3 | |
Regioni | 78 – 81 | Substrate bindingBy similarity | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4073, Eukaryota |
GeneTreei | ENSGT00390000007221 |
HOGENOMi | CLU_081974_3_2_1 |
InParanoidi | P61458 |
OMAi | WAEKWNH |
OrthoDBi | 1588292at2759 |
PhylomeDBi | P61458 |
TreeFami | TF300188 |
Family and domain databases
Gene3Di | 3.30.1360.20, 1 hit |
HAMAPi | MF_00434, Pterin_4_alpha, 1 hit |
InterProi | View protein in InterPro IPR036428, PCD_sf IPR001533, Pterin_deHydtase |
PANTHERi | PTHR12599, PTHR12599, 1 hit |
Pfami | View protein in Pfam PF01329, Pterin_4a, 1 hit |
SUPFAMi | SSF55248, SSF55248, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAGKAHRLSA EERDQLLPNL RAVGWNEVEG RDAIFKQFHF KDFNRAFGFM
60 70 80 90 100
TRVALQAEKL DHHPEWFNVY NKVHITLSTH ECAGLSERDI NLASFIEQVA
VSMT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1W2P7A4 | A0A1W2P7A4_MOUSE | 4a-hydroxytetrahydrobiopterin dehyd... | Pcbd1 | 60 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M83741 mRNA No translation available. AK007401 mRNA Translation: BAB25014.1 BC024354 mRNA Translation: AAH24354.1 |
CCDSi | CCDS35913.1 |
RefSeqi | NP_079549.1, NM_025273.4 |
Genome annotation databases
Ensembli | ENSMUST00000020298; ENSMUSP00000020298; ENSMUSG00000020098 |
GeneIDi | 13180 |
KEGGi | mmu:13180 |
UCSCi | uc007ffj.3, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M83741 mRNA No translation available. AK007401 mRNA Translation: BAB25014.1 BC024354 mRNA Translation: AAH24354.1 |
CCDSi | CCDS35913.1 |
RefSeqi | NP_079549.1, NM_025273.4 |
3D structure databases
AlphaFoldDBi | P61458 |
SMRi | P61458 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199068, 21 interactors |
IntActi | P61458, 17 interactors |
MINTi | P61458 |
STRINGi | 10090.ENSMUSP00000020298 |
PTM databases
iPTMneti | P61458 |
PhosphoSitePlusi | P61458 |
Proteomic databases
jPOSTi | P61458 |
MaxQBi | P61458 |
PaxDbi | P61458 |
PeptideAtlasi | P61458 |
PRIDEi | P61458 |
ProteomicsDBi | 288146 |
Protocols and materials databases
Antibodypediai | 29081, 237 antibodies from 28 providers |
DNASUi | 13180 |
Genome annotation databases
Ensembli | ENSMUST00000020298; ENSMUSP00000020298; ENSMUSG00000020098 |
GeneIDi | 13180 |
KEGGi | mmu:13180 |
UCSCi | uc007ffj.3, mouse |
Organism-specific databases
CTDi | 5092 |
MGIi | MGI:94873, Pcbd1 |
VEuPathDBi | HostDB:ENSMUSG00000020098 |
Phylogenomic databases
eggNOGi | KOG4073, Eukaryota |
GeneTreei | ENSGT00390000007221 |
HOGENOMi | CLU_081974_3_2_1 |
InParanoidi | P61458 |
OMAi | WAEKWNH |
OrthoDBi | 1588292at2759 |
PhylomeDBi | P61458 |
TreeFami | TF300188 |
Enzyme and pathway databases
Reactomei | R-MMU-8964208, Phenylalanine metabolism |
Miscellaneous databases
BioGRID-ORCSi | 13180, 1 hit in 75 CRISPR screens |
PROi | PR:P61458 |
RNActi | P61458, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020098, Expressed in right kidney and 249 other tissues |
Genevisiblei | P61458, MM |
Family and domain databases
Gene3Di | 3.30.1360.20, 1 hit |
HAMAPi | MF_00434, Pterin_4_alpha, 1 hit |
InterProi | View protein in InterPro IPR036428, PCD_sf IPR001533, Pterin_deHydtase |
PANTHERi | PTHR12599, PTHR12599, 1 hit |
Pfami | View protein in Pfam PF01329, Pterin_4a, 1 hit |
SUPFAMi | SSF55248, SSF55248, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PHS_MOUSE | |
Accessioni | P61458Primary (citable) accession number: P61458 Secondary accession number(s): P70519, P80095, Q9D930 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 24, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 141 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families