UniProtKB - P61374 (ISL1_RAT)
Insulin gene enhancer protein ISL-1
Isl1
Functioni
DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1 (By similarity).
Binds to insulin gene enhancer sequences (PubMed:1691825).
Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart (By similarity).
By similarity1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 181 – 240 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- bHLH transcription factor binding Source: RGD
- chromatin binding Source: RGD
- cis-regulatory region sequence-specific DNA binding Source: RGD
- DNA binding Source: RGD
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: RGD
- estrogen receptor binding Source: BHF-UCL
- metal ion binding Source: UniProtKB-KW
- nuclear receptor binding Source: BHF-UCL
- promoter-specific chromatin binding Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- RNA polymerase II-specific DNA-binding transcription factor binding Source: RGD
- sequence-specific DNA binding Source: RGD
- sequence-specific double-stranded DNA binding Source: RGD
GO - Biological processi
- atrial septum morphogenesis Source: RGD
- axonogenesis Source: GO_Central
- axon regeneration Source: RGD
- cardiac cell fate determination Source: RGD
- cardiac muscle cell myoblast differentiation Source: RGD
- cardiac right ventricle morphogenesis Source: RGD
- cellular response to glucocorticoid stimulus Source: BHF-UCL
- endocardial cushion morphogenesis Source: RGD
- heart development Source: UniProtKB
- heart morphogenesis Source: RGD
- innervation Source: RGD
- mesenchymal cell differentiation Source: RGD
- negative regulation of canonical Wnt signaling pathway Source: RGD
- negative regulation of epithelial cell proliferation Source: BHF-UCL
- negative regulation of inflammatory response Source: RGD
- negative regulation of intracellular estrogen receptor signaling pathway Source: BHF-UCL
- negative regulation of neuron apoptotic process Source: RGD
- negative regulation of neuron differentiation Source: RGD
- negative regulation of protein-containing complex assembly Source: BHF-UCL
- negative regulation of transcription by RNA polymerase II Source: RGD
- neural crest cell migration Source: RGD
- neuron differentiation Source: RGD
- neuron fate commitment Source: RGD
- neuron fate specification Source: RGD
- outflow tract morphogenesis Source: RGD
- outflow tract septum morphogenesis Source: RGD
- pancreas development Source: RGD
- peripheral nervous system neuron axonogenesis Source: RGD
- peripheral nervous system neuron development Source: RGD
- pharyngeal system development Source: RGD
- pituitary gland development Source: RGD
- positive regulation of angiogenesis Source: RGD
- positive regulation of cell differentiation Source: UniProtKB
- positive regulation of cell population proliferation Source: RGD
- positive regulation of DNA binding Source: RGD
- positive regulation of epithelial to mesenchymal transition Source: BHF-UCL
- positive regulation of granulocyte colony-stimulating factor production Source: RGD
- positive regulation of granulocyte macrophage colony-stimulating factor production Source: RGD
- positive regulation of histone acetylation Source: RGD
- positive regulation of insulin secretion Source: RGD
- positive regulation of interferon-gamma production Source: RGD
- positive regulation of interleukin-12 production Source: RGD
- positive regulation of interleukin-1 alpha production Source: RGD
- positive regulation of interleukin-1 beta production Source: RGD
- positive regulation of interleukin-6 production Source: RGD
- positive regulation of macrophage colony-stimulating factor production Source: RGD
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- positive regulation of tumor necrosis factor production Source: RGD
- positive regulation of tyrosine phosphorylation of STAT protein Source: RGD
- positive regulation of vascular endothelial growth factor production Source: RGD
- regulation of gene expression Source: RGD
- regulation of heart rate by cardiac conduction Source: RGD
- regulation of secondary heart field cardioblast proliferation Source: RGD
- regulation of transcription, DNA-templated Source: RGD
- regulation of transcription by RNA polymerase II Source: GO_Central
- retinal ganglion cell axon guidance Source: RGD
- secondary heart field specification Source: RGD
- sensory system development Source: RGD
- sinoatrial node cell development Source: RGD
- spinal cord motor neuron cell fate specification Source: RGD
- spinal cord motor neuron differentiation Source: RGD
- trigeminal nerve development Source: RGD
- ventricular cardiac muscle tissue morphogenesis Source: RGD
- visceral motor neuron differentiation Source: RGD
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding |
Biological process | Differentiation, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Insulin gene enhancer protein ISL-1Short name: Islet-1 |
Gene namesi | Name:Isl1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61957, Isl1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nucleus Source: RGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000075749 | 1 – 349 | Insulin gene enhancer protein ISL-1Add BLAST | 349 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P61374 |
PTM databases
PhosphoSitePlusi | P61374 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000012556, Expressed in stomach and 15 other tissues |
Genevisiblei | P61374, RN |
Interactioni
Subunit structurei
At neuronal promoters, displaces LDB1 from LHX3 LIM domain to form a ternary complex in which ISL1 contacts both LHX3 and LDB1.
Interacts (via C-terminus) with POU4F2 (via C-terminus).
Interacts with POU3F2.
Interacts with POU4F3.
Interacts (via N-terminal domain) with MLIP; the interaction represses ISL1 transactivator activity (By similarity).
By similarityGO - Molecular functioni
- bHLH transcription factor binding Source: RGD
- estrogen receptor binding Source: BHF-UCL
- nuclear receptor binding Source: BHF-UCL
- RNA polymerase II-specific DNA-binding transcription factor binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000017305 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 17 – 70 | LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST | 54 | |
Domaini | 79 – 133 | LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST | 55 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 262 – 291 | LIM-binding domain (LID)By similarityAdd BLAST | 30 | |
Regioni | 312 – 349 | DisorderedSequence analysisAdd BLAST | 38 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 312 – 341 | Polar residuesSequence analysisAdd BLAST | 30 |
Keywords - Domaini
Homeobox, LIM domain, RepeatPhylogenomic databases
eggNOGi | KOG0490, Eukaryota |
GeneTreei | ENSGT00940000153731 |
HOGENOMi | CLU_027802_2_0_1 |
InParanoidi | P61374 |
OMAi | VSQMHGY |
OrthoDBi | 948890at2759 |
PhylomeDBi | P61374 |
TreeFami | TF315442 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR001781, Znf_LIM |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00412, LIM, 2 hits |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00132, LIM, 2 hits |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS00478, LIM_DOMAIN_1, 2 hits PS50023, LIM_DOMAIN_2, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGDMGDPPKK KRLISLCVGC GNQIHDQYIL RVSPDLEWHA ACLKCAECNQ
60 70 80 90 100
YLDESCTCFV RDGKTYCKRD YIRLYGIKCA KCSIGFSKND FVMRARSKVY
110 120 130 140 150
HIECFRCVAC SRQLIPGDEF ALREDGLFCR ADHDVVERAS LGAGDPLSPL
160 170 180 190 200
HPARPLQMAA EPISARQPAL RPHVHKQPEK TTRVRTVLNE KQLHTLRTCY
210 220 230 240 250
AANPRPDALM KEQLVEMTGL SPRVIRVWFQ NKRCKDKKRS IMMKQLQQQQ
260 270 280 290 300
PNDKTNIQGM TGTPMVAASP ERHDGGLQAN PVEVQSYQPP WKVLSDFALQ
310 320 330 340
SDIDQPAFQQ LVNFSEGGPG SNSTGSEVAS MSSQLPDTPN SMVASPIEA
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A8I5Y041 | A0A8I5Y041_RAT | Insulin gene enhancer protein ISL-1 | Isl1 | 346 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 67 | C → S in AAS46773 (Ref. 2) Curated | 1 | |
Sequence conflicti | 199 | C → R in AAS46773 (Ref. 2) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_010339 | 256 – 278 | Missing in isoform 2. 1 PublicationAdd BLAST | 23 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S69329 mRNA Translation: AAB30128.1 AY557632 mRNA Translation: AAS46773.1 X53258 mRNA Translation: CAA37349.1 |
PIRi | I67417 |
RefSeqi | NP_059035.3, NM_017339.3 [P61374-1] |
Genome annotation databases
GeneIDi | 64444 |
KEGGi | rno:64444 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S69329 mRNA Translation: AAB30128.1 AY557632 mRNA Translation: AAS46773.1 X53258 mRNA Translation: CAA37349.1 |
PIRi | I67417 |
RefSeqi | NP_059035.3, NM_017339.3 [P61374-1] |
3D structure databases
AlphaFoldDBi | P61374 |
SMRi | P61374 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000017305 |
PTM databases
PhosphoSitePlusi | P61374 |
Proteomic databases
PaxDbi | P61374 |
Genome annotation databases
GeneIDi | 64444 |
KEGGi | rno:64444 |
Organism-specific databases
CTDi | 3670 |
RGDi | 61957, Isl1 |
Phylogenomic databases
eggNOGi | KOG0490, Eukaryota |
GeneTreei | ENSGT00940000153731 |
HOGENOMi | CLU_027802_2_0_1 |
InParanoidi | P61374 |
OMAi | VSQMHGY |
OrthoDBi | 948890at2759 |
PhylomeDBi | P61374 |
TreeFami | TF315442 |
Miscellaneous databases
PROi | PR:P61374 |
Gene expression databases
Bgeei | ENSRNOG00000012556, Expressed in stomach and 15 other tissues |
Genevisiblei | P61374, RN |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR001781, Znf_LIM |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00412, LIM, 2 hits |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00132, LIM, 2 hits |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS00478, LIM_DOMAIN_1, 2 hits PS50023, LIM_DOMAIN_2, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | ISL1_RAT | |
Accessioni | P61374Primary (citable) accession number: P61374 Secondary accession number(s): P20663, P47894 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 24, 2004 |
Last sequence update: | May 24, 2004 | |
Last modified: | May 25, 2022 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |