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Protein

Insulin gene enhancer protein ISL-1

Gene

ISL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1. Binds to insulin gene enhancer sequences. Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi181 – 240HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processDifferentiation, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-400511 Synthesis, secretion, and inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP)
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
SIGNORiP61371

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin gene enhancer protein ISL-1
Short name:
Islet-1
Gene namesi
Name:ISL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000016082.14
HGNCiHGNC:6132 ISL1
MIMi600366 gene
neXtProtiNX_P61371

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi3670
MalaCardsiISL1
OpenTargetsiENSG00000016082
Orphaneti93930 Bladder exstrophy
PharmGKBiPA29932

Polymorphism and mutation databases

BioMutaiISL1
DMDMi47606423

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000757461 – 349Insulin gene enhancer protein ISL-1Add BLAST349

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP61371
PaxDbiP61371
PeptideAtlasiP61371
PRIDEiP61371
ProteomicsDBi57299

PTM databases

iPTMnetiP61371
PhosphoSitePlusiP61371

Expressioni

Tissue specificityi

Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.1 Publication

Gene expression databases

BgeeiENSG00000016082 Expressed in 108 organ(s), highest expression level in forebrain
CleanExiHS_ISL1
ExpressionAtlasiP61371 baseline and differential
GenevisibleiP61371 HS

Organism-specific databases

HPAiHPA057416

Interactioni

Subunit structurei

At neuronal promoters, displaces LDB1 from LHX3 LIM domain to form a ternary complex in which ISL1 contacts both LHX3 and LDB1. Interacts (via C-terminus) with POU4F2 (via C-terminus). Interacts with POU3F2. Interacts with POU4F3. Interacts (via N-terminal domain) with MLIP; the interaction represses ISL1 transactivator activity (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ZNF511Q8NB153EBI-3906896,EBI-10269136

GO - Molecular functioni

Protein-protein interaction databases

BioGridi109877, 28 interactors
IntActiP61371, 10 interactors
MINTiP61371
STRINGi9606.ENSP00000230658

Structurei

3D structure databases

ProteinModelPortaliP61371
SMRiP61371
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 70LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST54
Domaini79 – 133LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST55

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni262 – 291LIM-binding domain (LID)By similarityAdd BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi241 – 349Gln-richAdd BLAST109

Keywords - Domaini

Homeobox, LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG0490 Eukaryota
ENOG410YIJ3 LUCA
GeneTreeiENSGT00760000118921
HOGENOMiHOG000236304
HOVERGENiHBG004671
InParanoidiP61371
KOiK09370
OMAiVSQMHGY
OrthoDBiEOG091G09L9
PhylomeDBiP61371
TreeFamiTF315442

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR001781 Znf_LIM
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF00412 LIM, 2 hits
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SM00132 LIM, 2 hits
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS00478 LIM_DOMAIN_1, 2 hits
PS50023 LIM_DOMAIN_2, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P61371-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGDMGDPPKK KRLISLCVGC GNQIHDQYIL RVSPDLEWHA ACLKCAECNQ
60 70 80 90 100
YLDESCTCFV RDGKTYCKRD YIRLYGIKCA KCSIGFSKND FVMRARSKVY
110 120 130 140 150
HIECFRCVAC SRQLIPGDEF ALREDGLFCR ADHDVVERAS LGAGDPLSPL
160 170 180 190 200
HPARPLQMAA EPISARQPAL RPHVHKQPEK TTRVRTVLNE KQLHTLRTCY
210 220 230 240 250
AANPRPDALM KEQLVEMTGL SPRVIRVWFQ NKRCKDKKRS IMMKQLQQQQ
260 270 280 290 300
PNDKTNIQGM TGTPMVAASP ERHDGGLQAN PVEVQSYQPP WKVLSDFALQ
310 320 330 340
SDIDQPAFQQ LVNFSEGGPG SNSTGSEVAS MSSQLPDTPN SMVASPIEA
Length:349
Mass (Da):39,036
Last modified:May 24, 2004 - v1
Checksum:i4DB82FD09154F404
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RBJ1D6RBJ1_HUMAN
Insulin gene enhancer protein ISL-1
ISL1
326Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S70721 mRNA Translation: AAD14064.1
U07559 mRNA Translation: AAA81946.1
BC031213 mRNA Translation: AAH31213.1
CCDSiCCDS43314.1
PIRiI53277
RefSeqiNP_002193.2, NM_002202.2
UniGeneiHs.505

Genome annotation databases

EnsembliENST00000230658; ENSP00000230658; ENSG00000016082
GeneIDi3670
KEGGihsa:3670
UCSCiuc003jor.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S70721 mRNA Translation: AAD14064.1
U07559 mRNA Translation: AAA81946.1
BC031213 mRNA Translation: AAH31213.1
CCDSiCCDS43314.1
PIRiI53277
RefSeqiNP_002193.2, NM_002202.2
UniGeneiHs.505

3D structure databases

ProteinModelPortaliP61371
SMRiP61371
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109877, 28 interactors
IntActiP61371, 10 interactors
MINTiP61371
STRINGi9606.ENSP00000230658

PTM databases

iPTMnetiP61371
PhosphoSitePlusiP61371

Polymorphism and mutation databases

BioMutaiISL1
DMDMi47606423

Proteomic databases

MaxQBiP61371
PaxDbiP61371
PeptideAtlasiP61371
PRIDEiP61371
ProteomicsDBi57299

Protocols and materials databases

DNASUi3670
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000230658; ENSP00000230658; ENSG00000016082
GeneIDi3670
KEGGihsa:3670
UCSCiuc003jor.4 human

Organism-specific databases

CTDi3670
DisGeNETi3670
EuPathDBiHostDB:ENSG00000016082.14
GeneCardsiISL1
HGNCiHGNC:6132 ISL1
HPAiHPA057416
MalaCardsiISL1
MIMi600366 gene
neXtProtiNX_P61371
OpenTargetsiENSG00000016082
Orphaneti93930 Bladder exstrophy
PharmGKBiPA29932
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0490 Eukaryota
ENOG410YIJ3 LUCA
GeneTreeiENSGT00760000118921
HOGENOMiHOG000236304
HOVERGENiHBG004671
InParanoidiP61371
KOiK09370
OMAiVSQMHGY
OrthoDBiEOG091G09L9
PhylomeDBiP61371
TreeFamiTF315442

Enzyme and pathway databases

ReactomeiR-HSA-400511 Synthesis, secretion, and inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP)
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
SIGNORiP61371

Miscellaneous databases

ChiTaRSiISL1 human
GeneWikiiISL1
GenomeRNAii3670
PROiPR:P61371
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000016082 Expressed in 108 organ(s), highest expression level in forebrain
CleanExiHS_ISL1
ExpressionAtlasiP61371 baseline and differential
GenevisibleiP61371 HS

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR001781 Znf_LIM
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF00412 LIM, 2 hits
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SM00132 LIM, 2 hits
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS00478 LIM_DOMAIN_1, 2 hits
PS50023 LIM_DOMAIN_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiISL1_HUMAN
AccessioniPrimary (citable) accession number: P61371
Secondary accession number(s): P20663, P47894
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: November 7, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  2. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
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