Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Proteasome activator complex subunit 3

Gene

Psme3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processApoptosis, Cell cycle

Enzyme and pathway databases

ReactomeiR-MMU-1169091 Activation of NF-kappaB in B cells
R-MMU-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-MMU-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-MMU-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-MMU-174113 SCF-beta-TrCP mediated degradation of Emi1
R-MMU-174154 APC/C:Cdc20 mediated degradation of Securin
R-MMU-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-MMU-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-MMU-187577 SCF(Skp2)-mediated degradation of p27/p21
R-MMU-195253 Degradation of beta-catenin by the destruction complex
R-MMU-202424 Downstream TCR signaling
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2871837 FCERI mediated NF-kB activation
R-MMU-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-MMU-350562 Regulation of ornithine decarboxylase (ODC)
R-MMU-382556 ABC-family proteins mediated transport
R-MMU-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-MMU-4608870 Asymmetric localization of PCP proteins
R-MMU-4641257 Degradation of AXIN
R-MMU-4641258 Degradation of DVL
R-MMU-5358346 Hedgehog ligand biogenesis
R-MMU-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-MMU-5607764 CLEC7A (Dectin-1) signaling
R-MMU-5610780 Degradation of GLI1 by the proteasome
R-MMU-5610785 GLI3 is processed to GLI3R by the proteasome
R-MMU-5632684 Hedgehog 'on' state
R-MMU-5658442 Regulation of RAS by GAPs
R-MMU-5668541 TNFR2 non-canonical NF-kB pathway
R-MMU-5676590 NIK-->noncanonical NF-kB signaling
R-MMU-5687128 MAPK6/MAPK4 signaling
R-MMU-5689603 UCH proteinases
R-MMU-5689880 Ub-specific processing proteases
R-MMU-68827 CDT1 association with the CDC6:ORC:origin complex
R-MMU-68949 Orc1 removal from chromatin
R-MMU-69017 CDK-mediated phosphorylation and removal of Cdc6
R-MMU-69229 Ubiquitin-dependent degradation of Cyclin D1
R-MMU-69481 G2/M Checkpoints
R-MMU-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-MMU-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-MMU-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-MMU-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-MMU-8939902 Regulation of RUNX2 expression and activity
R-MMU-8941858 Regulation of RUNX3 expression and activity
R-MMU-8948751 Regulation of PTEN stability and activity
R-MMU-8951664 Neddylation
R-MMU-9020702 Interleukin-1 signaling
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome activator complex subunit 3
Alternative name(s):
11S regulator complex subunit gamma
Short name:
REG-gamma
Activator of multicatalytic protease subunit 3
Ki nuclear autoantigen
Proteasome activator 28 subunit gamma
Short name:
PA28g
Short name:
PA28gamma
Gene namesi
Name:Psme3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1096366 Psme3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Disruption phenotypei

Growth retardation, small body size, and reduces significantly the percentage of cells that enter the S phase.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001617902 – 254Proteasome activator complex subunit 3Add BLAST253

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei17PhosphoserineBy similarity1
Modified residuei24PhosphoserineBy similarity1
Modified residuei195N6-acetyllysine; by P300/CBPBy similarity1
Modified residuei247Phosphoserine; by CHEK2By similarity1

Post-translational modificationi

Phosphorylated by MAP3K3. Phosphorylation at Ser-247 promotes its association with CCAR2 (By similarity).By similarity1 Publication
Acetylation at the major site Lys-195 is important for oligomerization and ability to degrade its target substrates. Deacetylated by SIRT1 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP61290
PaxDbiP61290
PeptideAtlasiP61290
PRIDEiP61290
TopDownProteomicsiP61290

2D gel databases

REPRODUCTION-2DPAGEiP61290

PTM databases

iPTMnetiP61290
PhosphoSitePlusiP61290

Expressioni

Gene expression databases

BgeeiENSMUSG00000078652 Expressed in 298 organ(s), highest expression level in blood
CleanExiMM_PSME3
ExpressionAtlasiP61290 baseline and differential
GenevisibleiP61290 MM

Interactioni

Subunit structurei

Homoheptamer; the stability of the heptamer is essential for the specific activation of the trypsine-like subunit and inhibition of the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits of the proteasome (By similarity). Interacts with p53/TP53 and MDM2 (By similarity). Interacts with MAP3K3 (PubMed:12650640). Associates with the proteasome (By similarity). Interacts with CCAR2 (By similarity). Interacts with FAM192A (via C-terminus); the interaction is direct and promotes the association of PSME3 with the 20S proteasome (By similarity). Interacts with COIL; the interaction is inhibited by FAM192A (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202435, 8 interactors
IntActiP61290, 3 interactors
STRINGi10090.ENSMUSP00000019470

Structurei

3D structure databases

ProteinModelPortaliP61290
SMRiP61290
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The C-terminal sequences affect heptamer stability and proteasome affinity.By similarity

Sequence similaritiesi

Belongs to the PA28 family.Curated

Phylogenomic databases

eggNOGiKOG4470 Eukaryota
ENOG410XQVX LUCA
GeneTreeiENSGT00510000046374
HOGENOMiHOG000282822
HOVERGENiHBG053745
InParanoidiP61290
KOiK06698
OMAiPCNKPLC
OrthoDBiEOG091G0GX7
PhylomeDBiP61290
TreeFamiTF106236

Family and domain databases

Gene3Di1.20.120.180, 1 hit
1.20.5.120, 1 hit
InterProiView protein in InterPro
IPR003186 PA28_C
IPR036997 PA28_C_sf
IPR036996 PA28_N_sf
IPR009077 Proteasome_activ_PA28
IPR003185 Proteasome_activ_PA28_N
IPR036252 Proteasome_activ_sf
PANTHERiPTHR10660 PTHR10660, 1 hit
PfamiView protein in Pfam
PF02251 PA28_alpha, 1 hit
PF02252 PA28_beta, 1 hit
SUPFAMiSSF47216 SSF47216, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P61290-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL
60 70 80 90 100
NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK
110 120 130 140 150
VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG
160 170 180 190 200
NNFGVSIQEE TVAELRTVES EAASYLDQIS RYYITRAKLV SKIAKYPHVE
210 220 230 240 250
DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE KIKRPRSSNA

ETLY
Length:254
Mass (Da):29,506
Last modified:May 10, 2004 - v1
Checksum:i116FAB47D60A26C0
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2A4J1A2A4J1_MOUSE
Proteasome activator complex subuni...
Psme3
203Annotation score:
A2A4J3A2A4J3_MOUSE
Proteasome activator complex subuni...
Psme3
154Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2A → L in AAC53297 (PubMed:9162094).Curated1
Sequence conflicti9Q → L in AAC53297 (PubMed:9162094).Curated1
Sequence conflicti23T → S in AAC53297 (PubMed:9162094).Curated1
Sequence conflicti117D → G in AAC53297 (PubMed:9162094).Curated1
Sequence conflicti172A → P in AAC53297 (PubMed:9162094).Curated1
Sequence conflicti188K → G in AAC53297 (PubMed:9162094).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87911 mRNA Translation: BAA22041.1
U60330 mRNA Translation: AAC53297.1
AB007139 Genomic DNA Translation: BAA28838.1
BC015255 mRNA Translation: AAH15255.1
CCDSiCCDS25463.1
RefSeqiNP_035322.1, NM_011192.3
UniGeneiMm.288477

Genome annotation databases

EnsembliENSMUST00000019470; ENSMUSP00000019470; ENSMUSG00000078652
GeneIDi19192
KEGGimmu:19192
UCSCiuc007lon.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87911 mRNA Translation: BAA22041.1
U60330 mRNA Translation: AAC53297.1
AB007139 Genomic DNA Translation: BAA28838.1
BC015255 mRNA Translation: AAH15255.1
CCDSiCCDS25463.1
RefSeqiNP_035322.1, NM_011192.3
UniGeneiMm.288477

3D structure databases

ProteinModelPortaliP61290
SMRiP61290
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202435, 8 interactors
IntActiP61290, 3 interactors
STRINGi10090.ENSMUSP00000019470

PTM databases

iPTMnetiP61290
PhosphoSitePlusiP61290

2D gel databases

REPRODUCTION-2DPAGEiP61290

Proteomic databases

EPDiP61290
PaxDbiP61290
PeptideAtlasiP61290
PRIDEiP61290
TopDownProteomicsiP61290

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019470; ENSMUSP00000019470; ENSMUSG00000078652
GeneIDi19192
KEGGimmu:19192
UCSCiuc007lon.1 mouse

Organism-specific databases

CTDi10197
MGIiMGI:1096366 Psme3

Phylogenomic databases

eggNOGiKOG4470 Eukaryota
ENOG410XQVX LUCA
GeneTreeiENSGT00510000046374
HOGENOMiHOG000282822
HOVERGENiHBG053745
InParanoidiP61290
KOiK06698
OMAiPCNKPLC
OrthoDBiEOG091G0GX7
PhylomeDBiP61290
TreeFamiTF106236

Enzyme and pathway databases

ReactomeiR-MMU-1169091 Activation of NF-kappaB in B cells
R-MMU-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-MMU-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-MMU-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-MMU-174113 SCF-beta-TrCP mediated degradation of Emi1
R-MMU-174154 APC/C:Cdc20 mediated degradation of Securin
R-MMU-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-MMU-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-MMU-187577 SCF(Skp2)-mediated degradation of p27/p21
R-MMU-195253 Degradation of beta-catenin by the destruction complex
R-MMU-202424 Downstream TCR signaling
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2871837 FCERI mediated NF-kB activation
R-MMU-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-MMU-350562 Regulation of ornithine decarboxylase (ODC)
R-MMU-382556 ABC-family proteins mediated transport
R-MMU-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-MMU-4608870 Asymmetric localization of PCP proteins
R-MMU-4641257 Degradation of AXIN
R-MMU-4641258 Degradation of DVL
R-MMU-5358346 Hedgehog ligand biogenesis
R-MMU-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-MMU-5607764 CLEC7A (Dectin-1) signaling
R-MMU-5610780 Degradation of GLI1 by the proteasome
R-MMU-5610785 GLI3 is processed to GLI3R by the proteasome
R-MMU-5632684 Hedgehog 'on' state
R-MMU-5658442 Regulation of RAS by GAPs
R-MMU-5668541 TNFR2 non-canonical NF-kB pathway
R-MMU-5676590 NIK-->noncanonical NF-kB signaling
R-MMU-5687128 MAPK6/MAPK4 signaling
R-MMU-5689603 UCH proteinases
R-MMU-5689880 Ub-specific processing proteases
R-MMU-68827 CDT1 association with the CDC6:ORC:origin complex
R-MMU-68949 Orc1 removal from chromatin
R-MMU-69017 CDK-mediated phosphorylation and removal of Cdc6
R-MMU-69229 Ubiquitin-dependent degradation of Cyclin D1
R-MMU-69481 G2/M Checkpoints
R-MMU-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-MMU-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-MMU-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-MMU-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-MMU-8939902 Regulation of RUNX2 expression and activity
R-MMU-8941858 Regulation of RUNX3 expression and activity
R-MMU-8948751 Regulation of PTEN stability and activity
R-MMU-8951664 Neddylation
R-MMU-9020702 Interleukin-1 signaling
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiPsme3 mouse
PROiPR:P61290
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000078652 Expressed in 298 organ(s), highest expression level in blood
CleanExiMM_PSME3
ExpressionAtlasiP61290 baseline and differential
GenevisibleiP61290 MM

Family and domain databases

Gene3Di1.20.120.180, 1 hit
1.20.5.120, 1 hit
InterProiView protein in InterPro
IPR003186 PA28_C
IPR036997 PA28_C_sf
IPR036996 PA28_N_sf
IPR009077 Proteasome_activ_PA28
IPR003185 Proteasome_activ_PA28_N
IPR036252 Proteasome_activ_sf
PANTHERiPTHR10660 PTHR10660, 1 hit
PfamiView protein in Pfam
PF02251 PA28_alpha, 1 hit
PF02252 PA28_beta, 1 hit
SUPFAMiSSF47216 SSF47216, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPSME3_MOUSE
AccessioniPrimary (citable) accession number: P61290
Secondary accession number(s): O35563
, P97373, Q12920, Q13172, Q9BQD9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: November 7, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again