PSME3

Functionⁱ

Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition (PubMed:25361978).7 Publications

GO - Molecular functionⁱ

endopeptidase activator activity
Source: UniProtKB
Inferred from direct assayⁱ
- 18309296
identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
MDM2/MDM4 family protein binding
Source: UniProtKB
Inferred from direct assayⁱ
- 18309296
p53 binding
Source: UniProtKB
Inferred from direct assayⁱ
- 18309296

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

apoptotic process Source: UniProtKB-KW
cell cycle Source: UniProtKB-KW
negative regulation of extrinsic apoptotic signaling pathway
Source: UniProtKB
Inferred from direct assayⁱ
- 18309296
positive regulation of endopeptidase activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
post-translational protein modification Source: Reactome
protein deubiquitination Source: Reactome
regulation of G1/S transition of mitotic cell cycle
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
regulation of proteasomal protein catabolic process
Source: UniProtKB
Inferred from direct assayⁱ
- 18309296

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Apoptosis, Cell cycle

Biological process

Apoptosis, Cell cycle

Enzyme and pathway databases

Reactomeⁱ	R-HSA-1169091 Activation of NF-kappaB in B cells R-HSA-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-HSA-1236974 ER-Phagosome pathway R-HSA-1236978 Cross-presentation of soluble exogenous antigens (endosomes) R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C R-HSA-174113 SCF-beta-TrCP mediated degradation of Emi1 R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-HSA-180534 Vpu mediated degradation of CD4 R-HSA-180585 Vif-mediated degradation of APOBEC3G R-HSA-187577 SCF(Skp2)-mediated degradation of p27/p21 R-HSA-195253 Degradation of beta-catenin by the destruction complex R-HSA-202424 Downstream TCR signaling R-HSA-211733 Regulation of activated PAK-2p34 by proteasome mediated degradation R-HSA-2467813 Separation of Sister Chromatids R-HSA-2871837 FCERI mediated NF-kB activation R-HSA-349425 Autodegradation of the E3 ubiquitin ligase COP1 R-HSA-350562 Regulation of ornithine decarboxylase (ODC) R-HSA-382556 ABC-family proteins mediated transport R-HSA-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA R-HSA-4608870 Asymmetric localization of PCP proteins R-HSA-4641257 Degradation of AXIN R-HSA-4641258 Degradation of DVL R-HSA-5358346 Hedgehog ligand biogenesis R-HSA-5362768 Hh mutants that don't undergo autocatalytic processing are degraded by ERAD R-HSA-5607761 Dectin-1 mediated noncanonical NF-kB signaling R-HSA-5607764 CLEC7A (Dectin-1) signaling R-HSA-5610780 Degradation of GLI1 by the proteasome R-HSA-5610783 Degradation of GLI2 by the proteasome R-HSA-5610785 GLI3 is processed to GLI3R by the proteasome R-HSA-5632684 Hedgehog 'on' state R-HSA-5658442 Regulation of RAS by GAPs R-HSA-5668541 TNFR2 non-canonical NF-kB pathway R-HSA-5676590 NIK-->noncanonical NF-kB signaling R-HSA-5678895 Defective CFTR causes cystic fibrosis R-HSA-5687128 MAPK6/MAPK4 signaling R-HSA-5689603 UCH proteinases R-HSA-5689880 Ub-specific processing proteases R-HSA-68827 CDT1 association with the CDC6:ORC:origin complex R-HSA-68949 Orc1 removal from chromatin R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6 R-HSA-69229 Ubiquitin-dependent degradation of Cyclin D1 R-HSA-69481 G2/M Checkpoints R-HSA-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-HSA-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint R-HSA-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-HSA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs R-HSA-8939902 Regulation of RUNX2 expression and activity R-HSA-8941858 Regulation of RUNX3 expression and activity R-HSA-8948751 Regulation of PTEN stability and activity R-HSA-8951664 Neddylation R-HSA-9010553 Regulation of expression of SLITs and ROBOs R-HSA-9020702 Interleukin-1 signaling R-HSA-9604323 Negative regulation of NOTCH4 signaling R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Reactomeⁱ

R-HSA-1169091 Activation of NF-kappaB in B cells
R-HSA-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-HSA-1236974 ER-Phagosome pathway
R-HSA-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-HSA-174113 SCF-beta-TrCP mediated degradation of Emi1
R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin
R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-180534 Vpu mediated degradation of CD4
R-HSA-180585 Vif-mediated degradation of APOBEC3G
R-HSA-187577 SCF(Skp2)-mediated degradation of p27/p21
R-HSA-195253 Degradation of beta-catenin by the destruction complex
R-HSA-202424 Downstream TCR signaling
R-HSA-211733 Regulation of activated PAK-2p34 by proteasome mediated degradation
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2871837 FCERI mediated NF-kB activation
R-HSA-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-HSA-350562 Regulation of ornithine decarboxylase (ODC)
R-HSA-382556 ABC-family proteins mediated transport
R-HSA-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-HSA-4608870 Asymmetric localization of PCP proteins
R-HSA-4641257 Degradation of AXIN
R-HSA-4641258 Degradation of DVL
R-HSA-5358346 Hedgehog ligand biogenesis
R-HSA-5362768 Hh mutants that don't undergo autocatalytic processing are degraded by ERAD
R-HSA-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-HSA-5607764 CLEC7A (Dectin-1) signaling
R-HSA-5610780 Degradation of GLI1 by the proteasome
R-HSA-5610783 Degradation of GLI2 by the proteasome
R-HSA-5610785 GLI3 is processed to GLI3R by the proteasome
R-HSA-5632684 Hedgehog 'on' state
R-HSA-5658442 Regulation of RAS by GAPs
R-HSA-5668541 TNFR2 non-canonical NF-kB pathway
R-HSA-5676590 NIK-->noncanonical NF-kB signaling
R-HSA-5678895 Defective CFTR causes cystic fibrosis
R-HSA-5687128 MAPK6/MAPK4 signaling
R-HSA-5689603 UCH proteinases
R-HSA-5689880 Ub-specific processing proteases
R-HSA-68827 CDT1 association with the CDC6:ORC:origin complex
R-HSA-68949 Orc1 removal from chromatin
R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-HSA-69229 Ubiquitin-dependent degradation of Cyclin D1
R-HSA-69481 G2/M Checkpoints
R-HSA-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-HSA-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-HSA-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-HSA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-HSA-8939902 Regulation of RUNX2 expression and activity
R-HSA-8941858 Regulation of RUNX3 expression and activity
R-HSA-8948751 Regulation of PTEN stability and activity
R-HSA-8951664 Neddylation
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-9020702 Interleukin-1 signaling
R-HSA-9604323 Negative regulation of NOTCH4 signaling
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Proteasome activator complex subunit 3 Alternative name(s): 11S regulator complex subunit gamma Short name: REG-gamma Activator of multicatalytic protease subunit 3 Ki nuclear autoantigen Proteasome activator 28 subunit gamma Short name: PA28g Short name: PA28gamma
Gene namesⁱ	Name:PSME3
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 17

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000131467.10
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:9570 PSME3
MIMⁱ	605129 gene
neXtProtⁱ	NX_P61289

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	188	K → E, D, A, C, N, Q, H, F, S, I or P: Assembles into less stable hexamers/heptamers and therefore impairs specificity of activation of trypsin-like subunits of the proteasome. 1 Publication		1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	10197
Open Targets More...OpenTargetsⁱ	ENSG00000131467
PharmGKBⁱ	PA33916

Polymorphism and mutation databases

BioMutaⁱ	PSME3
DMDMⁱ	47117724

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedCombined sources1 Publication
ChainⁱPRO_0000161789	2 – 254	Proteasome activator complex subunit 3Add BLAST		253

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylalanineCombined sources1 Publication	1
Modified residueⁱ	17	PhosphoserineCombined sources	1
Modified residueⁱ	24	PhosphoserineCombined sources	1
Modified residueⁱ	195	N6-acetyllysine; by P300/CBP1 Publication	1
Modified residueⁱ	247	Phosphoserine; by CHEK21 Publication	1

Post-translational modificationⁱ

Phosphorylated by MAP3K3 (By similarity). Phosphorylation at Ser-247 promotes its association with CCAR2.By similarity1 Publication

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	P61289
PaxDbⁱ	P61289
PeptideAtlas More...PeptideAtlasⁱ	P61289
PRIDEⁱ	P61289
ProteomicsDBⁱ	57290 57291 [P61289-2]

PTM databases

iPTMnetⁱ	P61289
PhosphoSitePlusⁱ	P61289
SwissPalmⁱ	P61289

Miscellaneous databases

PMAP-CutDBⁱ	P61289

PMAP-CutDBⁱ

P61289

Expressionⁱ

Inductionⁱ

Up-regulated in thyroid carcinoma cells.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000131467 Expressed in 226 organ(s), highest expression level in left coronary artery
CleanExⁱ	HS_PSME3
ExpressionAtlasⁱ	P61289 baseline and differential
Genevisibleⁱ	P61289 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB008388 HPA012510

HPAⁱ

CAB008388
HPA012510

Interactionⁱ

Subunit structureⁱ

Homoheptamer; the stability of the heptamer is essential for the specific activation of the trypsine-like subunit and inhibition of the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits of the proteasome. Interacts with p53/TP53 and MDM2. Interacts with MAP3K3 (By similarity). Associates with the proteasome. Interacts with CCAR2. Interacts with FAM192A (via C-terminus); the interaction is direct and promotes the association of PSME3 with the 20S proteasome (PubMed:29934401). Interacts with COIL; the interaction is inhibited by FAM192A (PubMed:29934401).By similarity8 Publications

(Microbial infection) Interacts with human cytomegalovirus UL27.1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
itself		7	EBI-355546,EBI-355546
	O93077	11	EBI-355546,EBI-9636511	From Hepacivirus C.
ABCF3	A0A0S2Z645	3	EBI-355546,EBI-16432404
ATN1	P54259	3	EBI-355546,EBI-945980
BBS2	Q9BXC9	6	EBI-355546,EBI-748297
COIL	P38432	5	EBI-355546,EBI-945751
DIP2A	Q14689-6	3	EBI-355546,EBI-10233719
EAF1	Q96JC9	8	EBI-355546,EBI-769261
EAF2	Q96CJ1	3	EBI-355546,EBI-1245604
FBXO7	Q9Y3I1	3	EBI-355546,EBI-1161222
MDM2	Q00987	8	EBI-355546,EBI-389668
NCOA3	Q9Y6Q9	5	EBI-355546,EBI-81196
PFDN5	Q99471	5	EBI-355546,EBI-357275
PRKAB2	O43741	7	EBI-355546,EBI-1053424
PRR13	Q9NZ81	5	EBI-355546,EBI-740924
SMURF1	Q9HCE7	5	EBI-355546,EBI-976466
SPG7	Q9UQ90	3	EBI-355546,EBI-717201
TNFAIP8L1	Q8WVP5	5	EBI-355546,EBI-752102
TP53	P04637	7	EBI-355546,EBI-366083
TXN2	Q99757	3	EBI-355546,EBI-2932492
vif	P12504	2	EBI-355546,EBI-779991	From Human immunodeficiency virus type 1 group M subtype B (isolate NY5).
WDYHV1	Q96HA8	4	EBI-355546,EBI-741158
ZCCHC10	Q8TBK6	6	EBI-355546,EBI-597063

GO - Molecular functionⁱ

identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
MDM2/MDM4 family protein binding
Source: UniProtKB
Inferred from direct assayⁱ
- 18309296
p53 binding
Source: UniProtKB
Inferred from direct assayⁱ
- 18309296

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	115492, 186 interactors
CORUMⁱ	P61289
IntActⁱ	P61289, 90 interactors
Molecular INTeraction database More...MINTⁱ	P61289
STRINGⁱ	9606.ENSP00000293362

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P61289
SMRⁱ	P61289
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domainⁱ

The C-terminal sequences affect heptamer stability and proteasome affinity.1 Publication

Sequence similaritiesⁱ

Belongs to the PA28 family.Curated

Phylogenomic databases

eggNOGⁱ	KOG4470 Eukaryota ENOG410XQVX LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00510000046374
HOVERGENⁱ	HBG053745
InParanoidⁱ	P61289
KEGG Orthology (KO) More...KOⁱ	K06698
OMAⁱ	PCNKPLC
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0GX7
PhylomeDBⁱ	P61289
TreeFamⁱ	TF106236

Family and domain databases

Gene3Dⁱ	1.20.120.180, 1 hit 1.20.5.120, 1 hit
InterProⁱ	View protein in InterPro IPR003186 PA28_C IPR036997 PA28_C_sf IPR036996 PA28_N_sf IPR009077 Proteasome_activ_PA28 IPR003185 Proteasome_activ_PA28_N IPR036252 Proteasome_activ_sf
PANTHERⁱ	PTHR10660 PTHR10660, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02251 PA28_alpha, 1 hit PF02252 PA28_beta, 1 hit
SUPFAMⁱ	SSF47216 SSF47216, 1 hit

Sequences (3+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 3 described isoforms and 10 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: P61289-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL 
        60         70         80         90        100
NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK 
       110        120        130        140        150
VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG 
       160        170        180        190        200
NNFGVSIQEE TVAELRTVES EAASYLDQIS RYYITRAKLV SKIAKYPHVE 
       210        220        230        240        250
DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE KIKRPRSSNA 

ETLY

Length:254

Mass (Da):29,506

Last modified:May 10, 2004 - v1

Checksum:ⁱ116FAB47D60A26C0

GO

Isoform 2 (identifier: P61289-2) [UniParc] [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
135-135: T → TPSGKGPHICFDLQ

« Hide

        10         20         30         40         50
MASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL 
        60         70         80         90        100
NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK 
       110        120        130        140        150
VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTPSGKG PHICFDLQVK 
       160        170        180        190        200
MWVQLLIPRI EDGNNFGVSI QEETVAELRT VESEAASYLD QISRYYITRA 
       210        220        230        240        250
KLVSKIAKYP HVEDYRRTVT EIDEKEYISL RLIISELRNQ YVTLHDMILK 
       260 
NIEKIKRPRS SNAETLY

Show »

Length:267

Mass (Da):30,887

Checksum:ⁱ76E7E6ECCC65589F

GO

Isoform 3 (identifier: P61289-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-13: MASLLKVDQEVKL → MEKWILKKIKYLQSGGLSASYYSY

« Hide

        10         20         30         40         50
MEKWILKKIK YLQSGGLSAS YYSYKVDSFR ERITSEAEDL VANFFPKKLL 
        60         70         80         90        100
ELDSFLKEPI LNIHDLTQIH SDMNLPVPDP ILLTNSHDGL DGPTYKKRRL 
       110        120        130        140        150
DECEEAFQGT KVFVMPNGML KSNQQLVDII EKVKPEIRLL IEKCNTVKMW 
       160        170        180        190        200
VQLLIPRIED GNNFGVSIQE ETVAELRTVE SEAASYLDQI SRYYITRAKL 
       210        220        230        240        250
VSKIAKYPHV EDYRRTVTEI DEKEYISLRL IISELRNQYV TLHDMILKNI 
       260 
EKIKRPRSSN AETLY

Note: No experimental confirmation. Gene prediction based on EST data.

Show »

Length:265

Mass (Da):30,890

Checksum:ⁱEC200E4F73756C82

GO

Computationally mapped potential isoform sequencesⁱ

There are 10 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

K9J957	K9J957_HUMAN	Proteasome activator complex subuni... Proteasome activator complex subunit 3 (REG gamma-3 variant)	PSME3	231	Annotation score:
K7ENH2	K7ENH2_HUMAN	Proteasome activator complex subuni... Proteasome activator complex subunit 3	PSME3	179	Annotation score:
B3KQ25	B3KQ25_HUMAN	cDNA FLJ32659 fis, clone TESTI10000... cDNA FLJ32659 fis, clone TESTI1000045, highly similar to Proteasome activator complex subunit 3	PSME3	193	Annotation score:
K7ESG5	K7ESG5_HUMAN	Proteasome activator complex subuni... Proteasome activator complex subunit 3	PSME3	198	Annotation score:
B7Z8D3	B7Z8D3_HUMAN	cDNA FLJ57249, highly similar to Pr... cDNA FLJ57249, highly similar to Proteasome activator complex subunit 3	PSME3	130	Annotation score:
K7EMD0	K7EMD0_HUMAN	Proteasome activator complex subuni... Proteasome activator complex subunit 3	PSME3	96	Annotation score:
A0A087WTV2	A0A087WTV2_HUMAN	Proteasome activator complex subuni... Proteasome activator complex subunit 3	PSME3	170	Annotation score:
K7EPX6	K7EPX6_HUMAN	Proteasome activator complex subuni... Proteasome activator complex subunit 3	PSME3	110	Annotation score:
K7EKR3	K7EKR3_HUMAN	Proteasome activator complex subuni... Proteasome activator complex subunit 3	PSME3	118	Annotation score:
K7EJF8	K7EJF8_HUMAN	Proteasome activator complex subuni... Proteasome activator complex subunit 3	PSME3	42	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	25	E → K in AAB60335 (PubMed:7951316).Curated		1
Sequence conflictⁱ	94	E → K in AAA93227 (PubMed:7545954).Curated		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_055047	1 – 13	MASLL…QEVKL → MEKWILKKIKYLQSGGLSAS YYSY in isoform 3. CuratedAdd BLAST		13
Alternative sequenceⁱVSP_004516	135	T → TPSGKGPHICFDLQ in isoform 2. 1 Publication		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U11292 mRNA Translation: AAB60335.1 BT019386 mRNA Translation: AAV38193.1 AK292618 mRNA Translation: BAF85307.1 AK074999 mRNA Translation: BAG52046.1 AC016889 Genomic DNA No translation available. CH471152 Genomic DNA Translation: EAW60893.1 BC001423 mRNA Translation: AAH01423.1 BC002684 mRNA Translation: AAH02684.1 BC008020 mRNA Translation: AAH08020.1 U25756 Genomic DNA Translation: AAA93227.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS11442.1 [P61289-2] CCDS45689.1 CCDS59290.1 [P61289-3]
PIRⁱ	I38702 A60537
NCBI Reference Sequences More...RefSeqⁱ	NP_005780.2, NM_005789.3 [P61289-1] NP_789839.1, NM_176863.2 [P61289-2]
UniGeneⁱ	Hs.152978

Genome annotation databases

Ensemblⁱ	ENST00000293362; ENSP00000293362; ENSG00000131467 [P61289-2] ENST00000441946; ENSP00000409487; ENSG00000131467 [P61289-3] ENST00000590720; ENSP00000466794; ENSG00000131467 [P61289-1]
GeneIDⁱ	10197
KEGGⁱ	hsa:10197
UCSC genome browser More...UCSCⁱ	uc002ibq.5 human

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P61289	Proteasome activator complex subunit 3		MACFA		254	UniRef100_P61289
Proteasome activator complex subunit 3		MOUSE		254
Proteasome activator complex subunit 3		PIG		254
Epididymis secretory protein Li 283		HUMAN		254
Proteasome activator complex subunit 3 isoform 1		CALJA		254
+43
P61289-2	Uncharacterized protein		MACMU		267	UniRef100_G7NIN5
PSME3 isoform 3		PANTR		267
Uncharacterized protein		RHIRO		267
Uncharacterized protein		PAPAN		267
Uncharacterized protein		CHLSB		267
+2
P61289-3	Proteasome (Prosome, macropain) activator subunit 3 (PA28 gamma Ki), isoform CRA_a		HUMAN		265	UniRef100_A0A024R203
PSME3 isoform 5		PANTR		265
Uncharacterized protein		MACMU		265
Uncharacterized protein		CERAT		265
Uncharacterized protein		PAPAN		265
+6

Protein	Similar proteins		Species	Score	Length	Source
P61289	Proteasome activator complex subunit 3		MACFA		254	UniRef90_P61289
Proteasome activator complex subunit 3		MOUSE		254
Proteasome activator complex subunit 3		PIG		254
Proteasome activator complex subunit 3		CHICK		254
Proteasome activator complex subunit 3		PONAB		254
+142
P61289-2	Proteasome activator subunit 3		CANLF		267	UniRef90_E2RPY9
Uncharacterized protein (Fragment)		Micrurus lemniscatus carvalhoi		287
Uncharacterized protein (Fragment)		MICCO		144
Proteasome activator complex subunit 3 (Fragment)		OPHHA		274
Proteasome activator subunit 3		CALJA		267
+31
P61289-3	Proteasome (Prosome, macropain) activator subunit 3 (PA28 gamma Ki), isoform CRA_a		HUMAN		265	UniRef90_A0A024R203
PSME3 isoform 5		PANTR		265
Uncharacterized protein		MACMU		265
Uncharacterized protein		CERAT		265
Uncharacterized protein		PAPAN		265
+23

Protein	Similar proteins		Species	Score	Length	Source
P61289	Proteasome activator complex subunit 3		MACFA		254	UniRef50_P61289
Proteasome activator complex subunit 3		MOUSE		254
Proteasome activator complex subunit 3		PIG		254
Proteasome activator complex subunit 3		PONAB		254
Proteasome activator complex subunit 3		CHICK		254
+309

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U11292 mRNA Translation: AAB60335.1 BT019386 mRNA Translation: AAV38193.1 AK292618 mRNA Translation: BAF85307.1 AK074999 mRNA Translation: BAG52046.1 AC016889 Genomic DNA No translation available. CH471152 Genomic DNA Translation: EAW60893.1 BC001423 mRNA Translation: AAH01423.1 BC002684 mRNA Translation: AAH02684.1 BC008020 mRNA Translation: AAH08020.1 U25756 Genomic DNA Translation: AAA93227.1
CCDSⁱ	CCDS11442.1 [P61289-2] CCDS45689.1 CCDS59290.1 [P61289-3]
PIRⁱ	I38702 A60537
RefSeqⁱ	NP_005780.2, NM_005789.3 [P61289-1] NP_789839.1, NM_176863.2 [P61289-2]
UniGeneⁱ	Hs.152978

3D structure databases

ProteinModelPortalⁱ	P61289
SMRⁱ	P61289
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	115492, 186 interactors
CORUMⁱ	P61289
IntActⁱ	P61289, 90 interactors
MINTⁱ	P61289
STRINGⁱ	9606.ENSP00000293362

PTM databases

iPTMnetⁱ	P61289
PhosphoSitePlusⁱ	P61289
SwissPalmⁱ	P61289

Polymorphism and mutation databases

BioMutaⁱ	PSME3
DMDMⁱ	47117724

Proteomic databases

EPDⁱ	P61289
PaxDbⁱ	P61289
PeptideAtlasⁱ	P61289
PRIDEⁱ	P61289
ProteomicsDBⁱ	57290 57291 [P61289-2]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	10197
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000293362; ENSP00000293362; ENSG00000131467 [P61289-2] ENST00000441946; ENSP00000409487; ENSG00000131467 [P61289-3] ENST00000590720; ENSP00000466794; ENSG00000131467 [P61289-1]
GeneIDⁱ	10197
KEGGⁱ	hsa:10197
UCSCⁱ	uc002ibq.5 human

Organism-specific databases

CTDⁱ	10197
DisGeNETⁱ	10197
EuPathDBⁱ	HostDB:ENSG00000131467.10
GeneCardsⁱ	PSME3
HGNCⁱ	HGNC:9570 PSME3
HPAⁱ	CAB008388 HPA012510
MIMⁱ	605129 gene
neXtProtⁱ	NX_P61289
OpenTargetsⁱ	ENSG00000131467
PharmGKBⁱ	PA33916
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG4470 Eukaryota ENOG410XQVX LUCA
GeneTreeⁱ	ENSGT00510000046374
HOVERGENⁱ	HBG053745
InParanoidⁱ	P61289
KOⁱ	K06698
OMAⁱ	PCNKPLC
OrthoDBⁱ	EOG091G0GX7
PhylomeDBⁱ	P61289
TreeFamⁱ	TF106236

Enzyme and pathway databases

Reactomeⁱ	R-HSA-1169091 Activation of NF-kappaB in B cells R-HSA-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-HSA-1236974 ER-Phagosome pathway R-HSA-1236978 Cross-presentation of soluble exogenous antigens (endosomes) R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C R-HSA-174113 SCF-beta-TrCP mediated degradation of Emi1 R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-HSA-180534 Vpu mediated degradation of CD4 R-HSA-180585 Vif-mediated degradation of APOBEC3G R-HSA-187577 SCF(Skp2)-mediated degradation of p27/p21 R-HSA-195253 Degradation of beta-catenin by the destruction complex R-HSA-202424 Downstream TCR signaling R-HSA-211733 Regulation of activated PAK-2p34 by proteasome mediated degradation R-HSA-2467813 Separation of Sister Chromatids R-HSA-2871837 FCERI mediated NF-kB activation R-HSA-349425 Autodegradation of the E3 ubiquitin ligase COP1 R-HSA-350562 Regulation of ornithine decarboxylase (ODC) R-HSA-382556 ABC-family proteins mediated transport R-HSA-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA R-HSA-4608870 Asymmetric localization of PCP proteins R-HSA-4641257 Degradation of AXIN R-HSA-4641258 Degradation of DVL R-HSA-5358346 Hedgehog ligand biogenesis R-HSA-5362768 Hh mutants that don't undergo autocatalytic processing are degraded by ERAD R-HSA-5607761 Dectin-1 mediated noncanonical NF-kB signaling R-HSA-5607764 CLEC7A (Dectin-1) signaling R-HSA-5610780 Degradation of GLI1 by the proteasome R-HSA-5610783 Degradation of GLI2 by the proteasome R-HSA-5610785 GLI3 is processed to GLI3R by the proteasome R-HSA-5632684 Hedgehog 'on' state R-HSA-5658442 Regulation of RAS by GAPs R-HSA-5668541 TNFR2 non-canonical NF-kB pathway R-HSA-5676590 NIK-->noncanonical NF-kB signaling R-HSA-5678895 Defective CFTR causes cystic fibrosis R-HSA-5687128 MAPK6/MAPK4 signaling R-HSA-5689603 UCH proteinases R-HSA-5689880 Ub-specific processing proteases R-HSA-68827 CDT1 association with the CDC6:ORC:origin complex R-HSA-68949 Orc1 removal from chromatin R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6 R-HSA-69229 Ubiquitin-dependent degradation of Cyclin D1 R-HSA-69481 G2/M Checkpoints R-HSA-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-HSA-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint R-HSA-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-HSA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs R-HSA-8939902 Regulation of RUNX2 expression and activity R-HSA-8941858 Regulation of RUNX3 expression and activity R-HSA-8948751 Regulation of PTEN stability and activity R-HSA-8951664 Neddylation R-HSA-9010553 Regulation of expression of SLITs and ROBOs R-HSA-9020702 Interleukin-1 signaling R-HSA-9604323 Negative regulation of NOTCH4 signaling R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Reactomeⁱ

R-HSA-1169091 Activation of NF-kappaB in B cells
R-HSA-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-HSA-1236974 ER-Phagosome pathway
R-HSA-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-HSA-174113 SCF-beta-TrCP mediated degradation of Emi1
R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin
R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-180534 Vpu mediated degradation of CD4
R-HSA-180585 Vif-mediated degradation of APOBEC3G
R-HSA-187577 SCF(Skp2)-mediated degradation of p27/p21
R-HSA-195253 Degradation of beta-catenin by the destruction complex
R-HSA-202424 Downstream TCR signaling
R-HSA-211733 Regulation of activated PAK-2p34 by proteasome mediated degradation
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2871837 FCERI mediated NF-kB activation
R-HSA-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-HSA-350562 Regulation of ornithine decarboxylase (ODC)
R-HSA-382556 ABC-family proteins mediated transport
R-HSA-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-HSA-4608870 Asymmetric localization of PCP proteins
R-HSA-4641257 Degradation of AXIN
R-HSA-4641258 Degradation of DVL
R-HSA-5358346 Hedgehog ligand biogenesis
R-HSA-5362768 Hh mutants that don't undergo autocatalytic processing are degraded by ERAD
R-HSA-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-HSA-5607764 CLEC7A (Dectin-1) signaling
R-HSA-5610780 Degradation of GLI1 by the proteasome
R-HSA-5610783 Degradation of GLI2 by the proteasome
R-HSA-5610785 GLI3 is processed to GLI3R by the proteasome
R-HSA-5632684 Hedgehog 'on' state
R-HSA-5658442 Regulation of RAS by GAPs
R-HSA-5668541 TNFR2 non-canonical NF-kB pathway
R-HSA-5676590 NIK-->noncanonical NF-kB signaling
R-HSA-5678895 Defective CFTR causes cystic fibrosis
R-HSA-5687128 MAPK6/MAPK4 signaling
R-HSA-5689603 UCH proteinases
R-HSA-5689880 Ub-specific processing proteases
R-HSA-68827 CDT1 association with the CDC6:ORC:origin complex
R-HSA-68949 Orc1 removal from chromatin
R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-HSA-69229 Ubiquitin-dependent degradation of Cyclin D1
R-HSA-69481 G2/M Checkpoints
R-HSA-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-HSA-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-HSA-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-HSA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-HSA-8939902 Regulation of RUNX2 expression and activity
R-HSA-8941858 Regulation of RUNX3 expression and activity
R-HSA-8948751 Regulation of PTEN stability and activity
R-HSA-8951664 Neddylation
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-9020702 Interleukin-1 signaling
R-HSA-9604323 Negative regulation of NOTCH4 signaling
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSⁱ	PSME3 human
GeneWikiⁱ	PSME3
GenomeRNAiⁱ	10197
PMAP-CutDBⁱ	P61289
Protein Ontology More...PROⁱ	PR:P61289
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000131467 Expressed in 226 organ(s), highest expression level in left coronary artery
CleanExⁱ	HS_PSME3
ExpressionAtlasⁱ	P61289 baseline and differential
Genevisibleⁱ	P61289 HS

Family and domain databases

Gene3Dⁱ	1.20.120.180, 1 hit 1.20.5.120, 1 hit
InterProⁱ	View protein in InterPro IPR003186 PA28_C IPR036997 PA28_C_sf IPR036996 PA28_N_sf IPR009077 Proteasome_activ_PA28 IPR003185 Proteasome_activ_PA28_N IPR036252 Proteasome_activ_sf
PANTHERⁱ	PTHR10660 PTHR10660, 1 hit
Pfamⁱ	View protein in Pfam PF02251 PA28_alpha, 1 hit PF02252 PA28_beta, 1 hit
SUPFAMⁱ	SSF47216 SSF47216, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PSME3_HUMAN
Accessionⁱ	P61289Primary (citable) accession number: P61289 Secondary accession number(s): A8K9A3 , O35563, P97373, Q12920, Q13172, Q9BQD9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
	Last sequence update:	May 10, 2004
	Last modified:	November 7, 2018
	This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM

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UniProtKB - P61289 (PSME3_HUMAN)

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Proteasome activator complex subunit 3

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

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Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

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Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

Miscellaneous databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

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Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

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Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ