UniProtKB - P61227 (RAP2B_RAT)
Protein
Ras-related protein Rap-2b
Gene
Rap2b
Organism
Rattus norvegicus (Rat)
Status
Functioni
Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells (By similarity).By similarity
Catalytic activityi
- EC:3.6.5.2By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 17 | GTPBy similarity | 8 | |
Nucleotide bindingi | 57 – 61 | GTPBy similarity | 5 | |
Nucleotide bindingi | 116 – 119 | GTPBy similarity | 4 |
GO - Molecular functioni
- GDP binding Source: RGD
- GTPase activity Source: GO_Central
- GTP binding Source: RGD
- protein domain specific binding Source: RGD
GO - Biological processi
- negative regulation of cell migration Source: RGD
- platelet activation Source: RGD
- platelet aggregation Source: RGD
- positive regulation of protein autophosphorylation Source: RGD
- Rap protein signal transduction Source: UniProtKB
- regulation of protein tyrosine kinase activity Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Rap2b |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620591, Rap2b |
Subcellular locationi
Endosome
Note: Associated with red blood cells-released vesicles.By similarity
Cytosol
- cytosol Source: RGD
Endosome
- recycling endosome Source: RGD
- recycling endosome membrane Source: UniProtKB
Extracellular region or secreted
- extracellular exosome Source: RGD
Plasma Membrane
- plasma membrane Source: RGD
Other locations
- bicellular tight junction Source: RGD
- cell-cell contact zone Source: RGD
- membrane Source: RGD
- membrane raft Source: RGD
Keywords - Cellular componenti
Endosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000030219 | 1 – 180 | Ras-related protein Rap-2bAdd BLAST | 180 | |
PropeptideiPRO_0000030220 | 181 – 183 | Removed in mature formBy similarity | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 176 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 177 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 180 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 180 | S-geranylgeranyl cysteineBy similarity | 1 |
Post-translational modificationi
Palmitoylated. Unlike RAP2A and RAP2C, palmitoylation of RAP2B is not required for association with recycling endosome membranes and activation of TNIK.By similarity
Keywords - PTMi
Lipoprotein, Methylation, Palmitate, PrenylationProteomic databases
PaxDbi | P61227 |
PRIDEi | P61227 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000014420, Expressed in frontal cortex and 21 other tissues |
Genevisiblei | P61227, RN |
Interactioni
Subunit structurei
Interacts with PLCE1.
Interacts with SGSM1, SGSM2 and SGSM3. The GTP-bound form of RAP2B interacts with RUNDC3A (By similarity).
By similarityGO - Molecular functioni
- protein domain specific binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 251033, 1 interactor |
STRINGi | 10116.ENSRNOP00000019340 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 32 – 40 | Effector regionCurated | 9 |
Domaini
The effector domain mediates the interaction with RUNDC3A.By similarity
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000160283 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | P61227 |
OMAi | YGHINEV |
OrthoDBi | 1353024at2759 |
PhylomeDBi | P61227 |
TreeFami | TF313014 |
Family and domain databases
CDDi | cd04176, Rap2, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR041840, Rap2 IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P61227-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MREYKVVVLG SGGVGKSALT VQFVTGSFIE KYDPTIEDFY RKEIEVDSSP
60 70 80 90 100
SVLEILDTAG TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI
110 120 130 140 150
IRVKRYERVP MILVGNKVDL EGEREVSYGE GKALAEEWSC PFMETSAKNK
160 170 180
ASVDELFAEI VRQMNYAAQP NGDEGCCSAC VIL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF386786 mRNA Translation: AAK66772.1 |
RefSeqi | NP_596901.1, NM_133410.1 |
Genome annotation databases
Ensembli | ENSRNOT00000019340; ENSRNOP00000019340; ENSRNOG00000014420 |
GeneIDi | 170923 |
KEGGi | rno:170923 |
UCSCi | RGD:620591, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF386786 mRNA Translation: AAK66772.1 |
RefSeqi | NP_596901.1, NM_133410.1 |
3D structure databases
SMRi | P61227 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 251033, 1 interactor |
STRINGi | 10116.ENSRNOP00000019340 |
Proteomic databases
PaxDbi | P61227 |
PRIDEi | P61227 |
Genome annotation databases
Ensembli | ENSRNOT00000019340; ENSRNOP00000019340; ENSRNOG00000014420 |
GeneIDi | 170923 |
KEGGi | rno:170923 |
UCSCi | RGD:620591, rat |
Organism-specific databases
CTDi | 5912 |
RGDi | 620591, Rap2b |
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000160283 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | P61227 |
OMAi | YGHINEV |
OrthoDBi | 1353024at2759 |
PhylomeDBi | P61227 |
TreeFami | TF313014 |
Enzyme and pathway databases
Reactomei | R-RNO-6798695, Neutrophil degranulation |
Miscellaneous databases
PROi | PR:P61227 |
Gene expression databases
Bgeei | ENSRNOG00000014420, Expressed in frontal cortex and 21 other tissues |
Genevisiblei | P61227, RN |
Family and domain databases
CDDi | cd04176, Rap2, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR041840, Rap2 IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAP2B_RAT | |
Accessioni | P61227Primary (citable) accession number: P61227 Secondary accession number(s): P17964, Q96EG5, Q9CXG0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2004 |
Last sequence update: | May 10, 2004 | |
Last modified: | December 2, 2020 | |
This is version 128 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families