UniProtKB - P61226 (RAP2B_MOUSE)
Protein
Ras-related protein Rap-2b
Gene
Rap2b
Organism
Mus musculus (Mouse)
Status
Functioni
Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells.1 Publication
Catalytic activityi
- EC:3.6.5.2By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 17 | GTPBy similarity | 8 | |
Nucleotide bindingi | 57 – 61 | GTPBy similarity | 5 | |
Nucleotide bindingi | 116 – 119 | GTPBy similarity | 4 |
GO - Molecular functioni
- GDP binding Source: MGI
- GTPase activity Source: GO_Central
- GTP binding Source: MGI
- protein domain specific binding Source: MGI
GO - Biological processi
- negative regulation of cell migration Source: MGI
- platelet activation Source: MGI
- platelet aggregation Source: MGI
- positive regulation of protein autophosphorylation Source: MGI
- Rap protein signal transduction Source: UniProtKB
- regulation of protein tyrosine kinase activity Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Rap2b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1921262, Rap2b |
Subcellular locationi
Endosome
- Recycling endosome membrane 1 Publication; Lipid-anchor 1 Publication; Cytoplasmic side 1 Publication
Note: Associated with red blood cells-released vesicles.By similarity
Cytosol
- cytosol Source: MGI
Endosome
- recycling endosome Source: MGI
- recycling endosome membrane Source: UniProtKB
Extracellular region or secreted
- extracellular exosome Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- bicellular tight junction Source: MGI
- cell-cell contact zone Source: MGI
- membrane Source: MGI
- membrane raft Source: MGI
Keywords - Cellular componenti
Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 176 | C → G: No reduction of association with the recycling endosome membranes and does activate TNIK; when associated with G-177. 1 Publication | 1 | |
Mutagenesisi | 177 | C → G: No reduction of association with the recycling endosome membranes and does activate TNIK; when associated with G-176. 1 Publication | 1 | |
Mutagenesisi | 180 | C → A: Loss of association with membranes. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000030217 | 1 – 180 | Ras-related protein Rap-2bAdd BLAST | 180 | |
PropeptideiPRO_0000030218 | 181 – 183 | Removed in mature form1 Publication | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 176 | S-palmitoyl cysteine1 Publication | 1 | |
Lipidationi | 177 | S-palmitoyl cysteine1 Publication | 1 | |
Modified residuei | 180 | Cysteine methyl ester1 Publication | 1 | |
Lipidationi | 180 | S-geranylgeranyl cysteine1 Publication | 1 |
Post-translational modificationi
Palmitoylated. Unlike RAP2A and RAP2C, palmitoylation of RAP2B is not required for association with recycling endosome membranes and activation of TNIK.1 Publication
Keywords - PTMi
Lipoprotein, Methylation, Palmitate, PrenylationProteomic databases
EPDi | P61226 |
jPOSTi | P61226 |
PaxDbi | P61226 |
PeptideAtlasi | P61226 |
PRIDEi | P61226 |
PTM databases
iPTMneti | P61226 |
PhosphoSitePlusi | P61226 |
SwissPalmi | P61226 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000036894, Expressed in cumulus cell and 269 other tissues |
Genevisiblei | P61226, MM |
Interactioni
Subunit structurei
Interacts with PLCE1.
Interacts with SGSM1, SGSM2 and SGSM3 (By similarity). The GTP-bound form of RAP2B interacts with RUNDC3A.
By similarity1 PublicationGO - Molecular functioni
- protein domain specific binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 216422, 6 interactors |
IntActi | P61226, 2 interactors |
STRINGi | 10090.ENSMUSP00000038841 |
Miscellaneous databases
RNActi | P61226, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 32 – 40 | Effector regionCurated | 9 |
Domaini
The effector domain mediates the interaction with RUNDC3A.1 Publication
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000160283 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | P61226 |
OMAi | YGHINEV |
OrthoDBi | 1353024at2759 |
PhylomeDBi | P61226 |
TreeFami | TF313014 |
Family and domain databases
CDDi | cd04176, Rap2, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR041840, Rap2 IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P61226-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MREYKVVVLG SGGVGKSALT VQFVTGSFIE KYDPTIEDFY RKEIEVDSSP
60 70 80 90 100
SVLEILDTAG TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI
110 120 130 140 150
IRVKRYERVP MILVGNKVDL EGEREVSYGE GKALAEEWSC PFMETSAKNK
160 170 180
ASVDELFAEI VRQMNYAAQP NGDEGCCSAC VIL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK014462 mRNA Translation: BAB29368.1 BC032168 mRNA Translation: AAH32168.1 BC046528 mRNA Translation: AAH46528.1 |
CCDSi | CCDS17378.1 |
RefSeqi | NP_082988.1, NM_028712.4 |
Genome annotation databases
Ensembli | ENSMUST00000049064; ENSMUSP00000038841; ENSMUSG00000036894 |
GeneIDi | 74012 |
KEGGi | mmu:74012 |
UCSCi | uc008pjl.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK014462 mRNA Translation: BAB29368.1 BC032168 mRNA Translation: AAH32168.1 BC046528 mRNA Translation: AAH46528.1 |
CCDSi | CCDS17378.1 |
RefSeqi | NP_082988.1, NM_028712.4 |
3D structure databases
SMRi | P61226 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 216422, 6 interactors |
IntActi | P61226, 2 interactors |
STRINGi | 10090.ENSMUSP00000038841 |
PTM databases
iPTMneti | P61226 |
PhosphoSitePlusi | P61226 |
SwissPalmi | P61226 |
Proteomic databases
EPDi | P61226 |
jPOSTi | P61226 |
PaxDbi | P61226 |
PeptideAtlasi | P61226 |
PRIDEi | P61226 |
Protocols and materials databases
Antibodypediai | 33622, 258 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000049064; ENSMUSP00000038841; ENSMUSG00000036894 |
GeneIDi | 74012 |
KEGGi | mmu:74012 |
UCSCi | uc008pjl.1, mouse |
Organism-specific databases
CTDi | 5912 |
MGIi | MGI:1921262, Rap2b |
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000160283 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | P61226 |
OMAi | YGHINEV |
OrthoDBi | 1353024at2759 |
PhylomeDBi | P61226 |
TreeFami | TF313014 |
Enzyme and pathway databases
Reactomei | R-MMU-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 74012, 2 hits in 19 CRISPR screens |
ChiTaRSi | Rap2b, mouse |
PROi | PR:P61226 |
RNActi | P61226, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036894, Expressed in cumulus cell and 269 other tissues |
Genevisiblei | P61226, MM |
Family and domain databases
CDDi | cd04176, Rap2, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR041840, Rap2 IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAP2B_MOUSE | |
Accessioni | P61226Primary (citable) accession number: P61226 Secondary accession number(s): P17964, Q96EG5, Q9CXG0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2004 |
Last sequence update: | May 10, 2004 | |
Last modified: | December 2, 2020 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families