Rap2b

Functionⁱ

Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells.1 Publication

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	10 – 17	GTPBy similarity	8
Nucleotide bindingⁱ	57 – 61	GTPBy similarity	5
Nucleotide bindingⁱ	116 – 119	GTPBy similarity	4

GO - Molecular functionⁱ

GDP binding Source: MGI
GTPase activity Source: InterPro
GTP binding Source: MGI
protein domain specific binding
Source: MGI
Inferred from physical interactionⁱ
- 16928684

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

negative regulation of cell migration
Source: MGI
Inferred from direct assayⁱ
- 19061864
platelet activation Source: MGI
platelet aggregation Source: MGI
positive regulation of protein autophosphorylation
Source: MGI
Inferred from direct assayⁱ
- 19061864
Rap protein signal transduction
Source: UniProtKB
Inferred from direct assayⁱ
- 19061864
regulation of protein tyrosine kinase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 19061864

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Ligand	GTP-binding, Nucleotide-binding

Ligand

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-MMU-6798695 Neutrophil degranulation

Reactomeⁱ

R-MMU-6798695 Neutrophil degranulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ras-related protein Rap-2b
Gene namesⁱ	Name:Rap2b
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 3

Organism-specific databases

MGIⁱ	MGI:1921262 Rap2b

Keywords - Cellular componentⁱ

Endosome, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	176	C → G: No reduction of association with the recycling endosome membranes and does activate TNIK; when associated with G-177. 1 Publication	1
Mutagenesisⁱ	177	C → G: No reduction of association with the recycling endosome membranes and does activate TNIK; when associated with G-176. 1 Publication	1
Mutagenesisⁱ	180	C → A: Loss of association with membranes. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000030217	1 – 180	Ras-related protein Rap-2bAdd BLAST		180
PropeptideⁱPRO_0000030218	181 – 183	Removed in mature form1 Publication		3

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Lipidationⁱ	176	S-palmitoyl cysteine1 Publication	1
Lipidationⁱ	177	S-palmitoyl cysteine1 Publication	1
Modified residueⁱ	180	Cysteine methyl ester1 Publication	1
Lipidationⁱ	180	S-geranylgeranyl cysteine1 Publication	1

Post-translational modificationⁱ

Palmitoylated. Unlike RAP2A and RAP2C, palmitoylation of RAP2B is not required for association with recycling endosome membranes and activation of TNIK.1 Publication

Keywords - PTMⁱ

Lipoprotein, Methylation, Palmitate, Prenylation

Proteomic databases

EPDⁱ	P61226
PaxDbⁱ	P61226
PeptideAtlas More...PeptideAtlasⁱ	P61226
PRIDEⁱ	P61226

PTM databases

iPTMnetⁱ	P61226
PhosphoSitePlusⁱ	P61226
SwissPalmⁱ	P61226

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000036894 Expressed in 254 organ(s), highest expression level in cumulus cell
CleanExⁱ	MM_RAP2B
ExpressionAtlasⁱ	P61226 baseline and differential
Genevisibleⁱ	P61226 MM

Interactionⁱ

Subunit structureⁱ

Interacts with PLCE1. Interacts with SGSM1, SGSM2 and SGSM3 (By similarity). The GTP-bound form of RAP2B interacts with RUNDC3A.By similarity1 Publication

GO - Molecular functionⁱ

protein domain specific binding
Source: MGI
Inferred from physical interactionⁱ
- 16928684

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	216422, 4 interactors
IntActⁱ	P61226, 2 interactors
STRINGⁱ	10090.ENSMUSP00000038841

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P61226
SMRⁱ	P61226
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	32 – 40	Effector regionCurated		9

Domainⁱ

The effector domain mediates the interaction with RUNDC3A.1 Publication

Sequence similaritiesⁱ

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0395 Eukaryota COG1100 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00860000133678
HOGENOMⁱ	HOG000233973
HOVERGENⁱ	HBG009351
InParanoidⁱ	P61226
KEGG Orthology (KO) More...KOⁱ	K07838
OMAⁱ	LADEWNC
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0OOG
PhylomeDBⁱ	P61226
TreeFamⁱ	TF313014

Family and domain databases

InterProⁱ	View protein in InterPro IPR027417 P-loop_NTPase IPR005225 Small_GTP-bd_dom IPR001806 Small_GTPase IPR020849 Small_GTPase_Ras-type
PANTHERⁱ	PTHR24070 PTHR24070, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00071 Ras, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit
TIGRFAMsⁱ	TIGR00231 small_GTP, 1 hit
PROSITEⁱ	View protein in PROSITE PS51421 RAS, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P61226-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MREYKVVVLG SGGVGKSALT VQFVTGSFIE KYDPTIEDFY RKEIEVDSSP 
        60         70         80         90        100
SVLEILDTAG TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI 
       110        120        130        140        150
IRVKRYERVP MILVGNKVDL EGEREVSYGE GKALAEEWSC PFMETSAKNK 
       160        170        180 
ASVDELFAEI VRQMNYAAQP NGDEGCCSAC VIL

Length:183

Mass (Da):20,504

Last modified:May 10, 2004 - v1

Checksum:ⁱA1139C2D5E7F5865

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK014462 mRNA Translation: BAB29368.1 BC032168 mRNA Translation: AAH32168.1 BC046528 mRNA Translation: AAH46528.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS17378.1
NCBI Reference Sequences More...RefSeqⁱ	NP_082988.1, NM_028712.4
UniGeneⁱ	Mm.422674 Mm.477648

Genome annotation databases

Ensemblⁱ	ENSMUST00000049064; ENSMUSP00000038841; ENSMUSG00000036894
GeneIDⁱ	74012
KEGGⁱ	mmu:74012
UCSC genome browser More...UCSCⁱ	uc008pjl.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P61226	Ras-related protein Rap-2b	)	HUMAN		183	UniRef100_P61225
Ras-related protein Rap-2a		PIG		183
Ras-related protein Rap-2b		RAT		183
RAP2B, member of RAS oncogene family		CALJA		183
RAP2B, member of RAS oncogene family		MACMU		183
+40

Protein	Similar proteins		Species	Score	Length	Source
P61226	Ras-related protein Rap-2b	)	HUMAN		183	UniRef90_P61225
Ras-related protein Rap-2a		PIG		183
Ras-related protein Rap-2b		RAT		183
RAP2B, member of RAS oncogene family		CALJA		183
RAP2B, member of RAS oncogene family		MACMU		183
+137

Protein	Similar proteins		Species	Score	Length	Source
P61226	Ras-related protein Rap-2a	)	HUMAN		183	UniRef50_P10114
Ras-related protein Rap-2a		PIG		183
Ras-related protein Rap-2b		RAT		183
Uncharacterized protein		MYOLU		183
Ras-related protein Rap-2a		MYODS		117
+280

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK014462 mRNA Translation: BAB29368.1 BC032168 mRNA Translation: AAH32168.1 BC046528 mRNA Translation: AAH46528.1
CCDSⁱ	CCDS17378.1
RefSeqⁱ	NP_082988.1, NM_028712.4
UniGeneⁱ	Mm.422674 Mm.477648

3D structure databases

ProteinModelPortalⁱ	P61226
SMRⁱ	P61226
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	216422, 4 interactors
IntActⁱ	P61226, 2 interactors
STRINGⁱ	10090.ENSMUSP00000038841

PTM databases

iPTMnetⁱ	P61226
PhosphoSitePlusⁱ	P61226
SwissPalmⁱ	P61226

Proteomic databases

EPDⁱ	P61226
PaxDbⁱ	P61226
PeptideAtlasⁱ	P61226
PRIDEⁱ	P61226

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000049064; ENSMUSP00000038841; ENSMUSG00000036894
GeneIDⁱ	74012
KEGGⁱ	mmu:74012
UCSCⁱ	uc008pjl.1 mouse

Organism-specific databases

CTDⁱ	5912
MGIⁱ	MGI:1921262 Rap2b

Phylogenomic databases

eggNOGⁱ	KOG0395 Eukaryota COG1100 LUCA
GeneTreeⁱ	ENSGT00860000133678
HOGENOMⁱ	HOG000233973
HOVERGENⁱ	HBG009351
InParanoidⁱ	P61226
KOⁱ	K07838
OMAⁱ	LADEWNC
OrthoDBⁱ	EOG091G0OOG
PhylomeDBⁱ	P61226
TreeFamⁱ	TF313014

Enzyme and pathway databases

Reactomeⁱ	R-MMU-6798695 Neutrophil degranulation

Reactomeⁱ

R-MMU-6798695 Neutrophil degranulation

Miscellaneous databases

ChiTaRSⁱ	Rap2b mouse
Protein Ontology More...PROⁱ	PR:P61226
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000036894 Expressed in 254 organ(s), highest expression level in cumulus cell
CleanExⁱ	MM_RAP2B
ExpressionAtlasⁱ	P61226 baseline and differential
Genevisibleⁱ	P61226 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR027417 P-loop_NTPase IPR005225 Small_GTP-bd_dom IPR001806 Small_GTPase IPR020849 Small_GTPase_Ras-type
PANTHERⁱ	PTHR24070 PTHR24070, 1 hit
Pfamⁱ	View protein in Pfam PF00071 Ras, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit
TIGRFAMsⁱ	TIGR00231 small_GTP, 1 hit
PROSITEⁱ	View protein in PROSITE PS51421 RAS, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RAP2B_MOUSE
Accessionⁱ	P61226Primary (citable) accession number: P61226 Secondary accession number(s): P17964, Q96EG5, Q9CXG0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
	Last sequence update:	May 10, 2004
	Last modified:	September 12, 2018
	This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P61226 (RAP2B_MOUSE)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Ras-related protein Rap-2b

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endosome

Cytosol

Endosome

Extracellular region or secreted

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ