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Protein

Ras-related protein Rap-2b

Gene

RAP2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17GTPBy similarity8
Nucleotide bindingi57 – 61GTPBy similarity5
Nucleotide bindingi116 – 119GTPBy similarity4

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB
  • protein domain specific binding Source: Ensembl

GO - Biological processi

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rap-2b
Gene namesi
Name:RAP2B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000181467.3
HGNCiHGNC:9862 RAP2B
MIMi179541 gene
neXtProtiNX_P61225

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi5912
OpenTargetsiENSG00000181467
PharmGKBiPA34223

Polymorphism and mutation databases

DMDMi47117762

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000302151 – 180Ras-related protein Rap-2bAdd BLAST180
PropeptideiPRO_0000030216181 – 183Removed in mature form1 Publication3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi176S-palmitoyl cysteineBy similarity1
Lipidationi177S-palmitoyl cysteineBy similarity1
Modified residuei180Cysteine methyl ester1 Publication1
Lipidationi180S-geranylgeranyl cysteine1 Publication1

Post-translational modificationi

Palmitoylated. Unlike RAP2A and RAP2C, palmitoylation of RAP2B is not required for association with recycling endosome membranes and activation of TNIK.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Palmitate, Prenylation

Proteomic databases

EPDiP61225
MaxQBiP61225
PaxDbiP61225
PeptideAtlasiP61225
PRIDEiP61225
ProteomicsDBi57278

PTM databases

iPTMnetiP61225
PhosphoSitePlusiP61225
SwissPalmiP61225

Expressioni

Tissue specificityi

Expressed in red blood cells (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000181467 Expressed in 233 organ(s), highest expression level in tendon
CleanExiHS_RAP2B
GenevisibleiP61225 HS

Interactioni

Subunit structurei

Interacts with PLCE1. Interacts with SGSM1, SGSM2 and SGSM3. The GTP-bound form of RAP2B interacts with RUNDC3A (By similarity).By similarity

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111847, 14 interactors
IntActiP61225, 9 interactors
STRINGi9606.ENSP00000319096

Structurei

3D structure databases

ProteinModelPortaliP61225
SMRiP61225
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector regionCurated9

Domaini

The effector domain mediates the interaction with RUNDC3A.By similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00860000133678
HOGENOMiHOG000233973
HOVERGENiHBG009351
InParanoidiP61225
KOiK07838
OMAiLADEWNC
OrthoDBiEOG091G0OOG
PhylomeDBiP61225
TreeFamiTF313014

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras-type
PANTHERiPTHR24070 PTHR24070, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61225-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MREYKVVVLG SGGVGKSALT VQFVTGSFIE KYDPTIEDFY RKEIEVDSSP
60 70 80 90 100
SVLEILDTAG TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI
110 120 130 140 150
IRVKRYERVP MILVGNKVDL EGEREVSYGE GKALAEEWSC PFMETSAKNK
160 170 180
ASVDELFAEI VRQMNYAAQP NGDEGCCSAC VIL
Length:183
Mass (Da):20,504
Last modified:May 10, 2004 - v1
Checksum:iA1139C2D5E7F5865
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti170P → S in CAA37178 (PubMed:2115998).Curated1
Sequence conflicti170P → S no nucleotide entry (PubMed:2118648).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52987 mRNA Translation: CAA37178.1
AF493915 mRNA Translation: AAM12629.1
BC012362 mRNA Translation: AAH12362.1
CCDSiCCDS3170.1
PIRiA33121 TVHUR2
RefSeqiNP_002877.2, NM_002886.3
UniGeneiHs.700713
Hs.98643

Genome annotation databases

EnsembliENST00000323534; ENSP00000319096; ENSG00000181467
GeneIDi5912
KEGGihsa:5912
UCSCiuc003ezr.5 human

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52987 mRNA Translation: CAA37178.1
AF493915 mRNA Translation: AAM12629.1
BC012362 mRNA Translation: AAH12362.1
CCDSiCCDS3170.1
PIRiA33121 TVHUR2
RefSeqiNP_002877.2, NM_002886.3
UniGeneiHs.700713
Hs.98643

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1N4PX-ray2.65M/N180-183[»]
1N4QX-ray2.40M/N/O/P/Q/R180-183[»]
1N4RX-ray2.80M/N/O/P/Q/R180-183[»]
ProteinModelPortaliP61225
SMRiP61225
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111847, 14 interactors
IntActiP61225, 9 interactors
STRINGi9606.ENSP00000319096

PTM databases

iPTMnetiP61225
PhosphoSitePlusiP61225
SwissPalmiP61225

Polymorphism and mutation databases

DMDMi47117762

Proteomic databases

EPDiP61225
MaxQBiP61225
PaxDbiP61225
PeptideAtlasiP61225
PRIDEiP61225
ProteomicsDBi57278

Protocols and materials databases

DNASUi5912
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000323534; ENSP00000319096; ENSG00000181467
GeneIDi5912
KEGGihsa:5912
UCSCiuc003ezr.5 human

Organism-specific databases

CTDi5912
DisGeNETi5912
EuPathDBiHostDB:ENSG00000181467.3
GeneCardsiRAP2B
HGNCiHGNC:9862 RAP2B
MIMi179541 gene
neXtProtiNX_P61225
OpenTargetsiENSG00000181467
PharmGKBiPA34223
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0395 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00860000133678
HOGENOMiHOG000233973
HOVERGENiHBG009351
InParanoidiP61225
KOiK07838
OMAiLADEWNC
OrthoDBiEOG091G0OOG
PhylomeDBiP61225
TreeFamiTF313014

Enzyme and pathway databases

ReactomeiR-HSA-6798695 Neutrophil degranulation

Miscellaneous databases

ChiTaRSiRAP2B human
GeneWikiiRAP2B
GenomeRNAii5912
PROiPR:P61225
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000181467 Expressed in 233 organ(s), highest expression level in tendon
CleanExiHS_RAP2B
GenevisibleiP61225 HS

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras-type
PANTHERiPTHR24070 PTHR24070, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAP2B_HUMAN
AccessioniPrimary (citable) accession number: P61225
Secondary accession number(s): P17964, Q96EG5, Q9CXG0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: September 12, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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