UniProtKB - P61225 (RAP2B_HUMAN)
Protein
Ras-related protein Rap-2b
Gene
RAP2B
Organism
Homo sapiens (Human)
Status
Functioni
Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells.3 Publications
Catalytic activityi
- EC:3.6.5.2By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 17 | GTPBy similarity | 8 | |
Nucleotide bindingi | 57 – 61 | GTPBy similarity | 5 | |
Nucleotide bindingi | 116 – 119 | GTPBy similarity | 4 |
GO - Molecular functioni
- GDP binding Source: UniProtKB
- GTPase activity Source: GO_Central
- GTP binding Source: UniProtKB
- protein domain specific binding Source: Ensembl
GO - Biological processi
- negative regulation of cell migration Source: GO_Central
- neutrophil degranulation Source: Reactome
- platelet activation Source: UniProtKB
- platelet aggregation Source: UniProtKB
- positive regulation of protein autophosphorylation Source: Ensembl
- Rap protein signal transduction Source: UniProtKB
- regulation of protein tyrosine kinase activity Source: UniProtKB
- signal transduction Source: ProtInc
Keywordsi
Molecular function | Hydrolase |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P61225 |
Reactomei | R-HSA-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:RAP2B |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9862, RAP2B |
MIMi | 179541, gene |
neXtProti | NX_P61225 |
VEuPathDBi | HostDB:ENSG00000181467.3 |
Subcellular locationi
Endosome
- Recycling endosome membrane 1 Publication; Lipid-anchor 1 Publication; Cytoplasmic side 1 Publication
Note: Associated with red blood cells-released vesicles.
Cytosol
- cytosol Source: UniProtKB
Endosome
- recycling endosome membrane Source: UniProtKB
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- bicellular tight junction Source: UniProtKB
- cell-cell contact zone Source: UniProtKB
- membrane Source: UniProtKB
- membrane raft Source: UniProtKB
- specific granule membrane Source: Reactome
- tertiary granule membrane Source: Reactome
Keywords - Cellular componenti
Endosome, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 5912 |
OpenTargetsi | ENSG00000181467 |
PharmGKBi | PA34223 |
Miscellaneous databases
Pharosi | P61225, Tbio |
Genetic variation databases
BioMutai | RAP2B |
DMDMi | 47117762 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000030215 | 1 – 180 | Ras-related protein Rap-2bAdd BLAST | 180 | |
PropeptideiPRO_0000030216 | 181 – 183 | Removed in mature form1 Publication | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 176 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 177 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 180 | Cysteine methyl ester1 Publication | 1 | |
Lipidationi | 180 | S-geranylgeranyl cysteine1 Publication | 1 |
Post-translational modificationi
Palmitoylated. Unlike RAP2A and RAP2C, palmitoylation of RAP2B is not required for association with recycling endosome membranes and activation of TNIK.By similarity
Keywords - PTMi
Lipoprotein, Methylation, Palmitate, PrenylationProteomic databases
EPDi | P61225 |
jPOSTi | P61225 |
MassIVEi | P61225 |
MaxQBi | P61225 |
PaxDbi | P61225 |
PeptideAtlasi | P61225 |
PRIDEi | P61225 |
ProteomicsDBi | 57278 |
PTM databases
iPTMneti | P61225 |
MetOSitei | P61225 |
PhosphoSitePlusi | P61225 |
SwissPalmi | P61225 |
Expressioni
Tissue specificityi
Expressed in red blood cells (at protein level).1 Publication
Gene expression databases
Bgeei | ENSG00000181467, Expressed in tendon and 245 other tissues |
Genevisiblei | P61225, HS |
Organism-specific databases
HPAi | ENSG00000181467, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with PLCE1.
Interacts with SGSM1, SGSM2 and SGSM3. The GTP-bound form of RAP2B interacts with RUNDC3A (By similarity).
By similarityBinary interactionsi
Hide detailsP61225
GO - Molecular functioni
- protein domain specific binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 111847, 19 interactors |
IntActi | P61225, 9 interactors |
STRINGi | 9606.ENSP00000319096 |
Miscellaneous databases
RNActi | P61225, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 32 – 40 | Effector regionCurated | 9 |
Domaini
The effector domain mediates the interaction with RUNDC3A.By similarity
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000160283 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | P61225 |
OMAi | YGHINEV |
OrthoDBi | 1353024at2759 |
PhylomeDBi | P61225 |
TreeFami | TF313014 |
Family and domain databases
CDDi | cd04176, Rap2, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR041840, Rap2 IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P61225-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MREYKVVVLG SGGVGKSALT VQFVTGSFIE KYDPTIEDFY RKEIEVDSSP
60 70 80 90 100
SVLEILDTAG TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI
110 120 130 140 150
IRVKRYERVP MILVGNKVDL EGEREVSYGE GKALAEEWSC PFMETSAKNK
160 170 180
ASVDELFAEI VRQMNYAAQP NGDEGCCSAC VIL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 170 | P → S in CAA37178 (PubMed:2115998).Curated | 1 | |
Sequence conflicti | 170 | P → S no nucleotide entry (PubMed:2118648).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52987 mRNA Translation: CAA37178.1 AF493915 mRNA Translation: AAM12629.1 BC012362 mRNA Translation: AAH12362.1 |
CCDSi | CCDS3170.1 |
PIRi | A33121, TVHUR2 |
RefSeqi | NP_002877.2, NM_002886.3 |
Genome annotation databases
Ensembli | ENST00000323534; ENSP00000319096; ENSG00000181467 |
GeneIDi | 5912 |
KEGGi | hsa:5912 |
UCSCi | uc003ezr.5, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52987 mRNA Translation: CAA37178.1 AF493915 mRNA Translation: AAM12629.1 BC012362 mRNA Translation: AAH12362.1 |
CCDSi | CCDS3170.1 |
PIRi | A33121, TVHUR2 |
RefSeqi | NP_002877.2, NM_002886.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1N4P | X-ray | 2.65 | M/N | 180-183 | [»] | |
1N4Q | X-ray | 2.40 | M/N/O/P/Q/R | 180-183 | [»] | |
1N4R | X-ray | 2.80 | M/N/O/P/Q/R | 180-183 | [»] | |
SMRi | P61225 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111847, 19 interactors |
IntActi | P61225, 9 interactors |
STRINGi | 9606.ENSP00000319096 |
PTM databases
iPTMneti | P61225 |
MetOSitei | P61225 |
PhosphoSitePlusi | P61225 |
SwissPalmi | P61225 |
Genetic variation databases
BioMutai | RAP2B |
DMDMi | 47117762 |
Proteomic databases
EPDi | P61225 |
jPOSTi | P61225 |
MassIVEi | P61225 |
MaxQBi | P61225 |
PaxDbi | P61225 |
PeptideAtlasi | P61225 |
PRIDEi | P61225 |
ProteomicsDBi | 57278 |
Protocols and materials databases
Antibodypediai | 33622, 258 antibodies |
DNASUi | 5912 |
Genome annotation databases
Ensembli | ENST00000323534; ENSP00000319096; ENSG00000181467 |
GeneIDi | 5912 |
KEGGi | hsa:5912 |
UCSCi | uc003ezr.5, human |
Organism-specific databases
CTDi | 5912 |
DisGeNETi | 5912 |
GeneCardsi | RAP2B |
HGNCi | HGNC:9862, RAP2B |
HPAi | ENSG00000181467, Low tissue specificity |
MIMi | 179541, gene |
neXtProti | NX_P61225 |
OpenTargetsi | ENSG00000181467 |
PharmGKBi | PA34223 |
VEuPathDBi | HostDB:ENSG00000181467.3 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000160283 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | P61225 |
OMAi | YGHINEV |
OrthoDBi | 1353024at2759 |
PhylomeDBi | P61225 |
TreeFami | TF313014 |
Enzyme and pathway databases
PathwayCommonsi | P61225 |
Reactomei | R-HSA-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 5912, 9 hits in 872 CRISPR screens |
ChiTaRSi | RAP2B, human |
GeneWikii | RAP2B |
GenomeRNAii | 5912 |
Pharosi | P61225, Tbio |
PROi | PR:P61225 |
RNActi | P61225, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000181467, Expressed in tendon and 245 other tissues |
Genevisiblei | P61225, HS |
Family and domain databases
CDDi | cd04176, Rap2, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR041840, Rap2 IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAP2B_HUMAN | |
Accessioni | P61225Primary (citable) accession number: P61225 Secondary accession number(s): P17964, Q96EG5, Q9CXG0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2004 |
Last sequence update: | May 10, 2004 | |
Last modified: | February 10, 2021 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families