Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ras-related protein Rap-1b

Gene

RAP1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function.3 Publications

Activity regulationi

Activated by binding to the GTPase-activating protein RAP1GAP. Activated by guanine nucleotide-exchange factor (GEF) EPAC2 in a cAMP-dependent manner.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18GTP2 Publications9
Nucleotide bindingi57 – 61GTP2 Publications5
Nucleotide bindingi116 – 119GTP2 Publications4
Nucleotide bindingi147 – 149GTP2 Publications3

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB
  • protein-containing complex binding Source: MGI

GO - Biological processi

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-354192 Integrin alphaIIb beta3 signaling
R-HSA-354194 GRB2:SOS provides linkage to MAPK signaling for Integrins
R-HSA-372708 p130Cas linkage to MAPK signaling for integrins
R-HSA-392517 Rap1 signalling
R-HSA-5674135 MAP2K and MAPK activation
R-HSA-6798695 Neutrophil degranulation
R-HSA-6802946 Signaling by moderate kinase activity BRAF mutants
R-HSA-6802948 Signaling by high-kinase activity BRAF mutants
R-HSA-6802949 Signaling by RAS mutants
R-HSA-6802952 Signaling by BRAF and RAF fusions
R-HSA-6802955 Paradoxical activation of RAF signaling by kinase inactive BRAF
R-HSA-8875555 MET activates RAP1 and RAC1
R-HSA-8950505 Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation
SignaLinkiP61224
SIGNORiP61224

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rap-1b
Alternative name(s):
GTP-binding protein smg p21B
Gene namesi
Name:RAP1B
ORF Names:OK/SW-cl.11
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000127314.17
HGNCiHGNC:9857 RAP1B
MIMi179530 gene
neXtProtiNX_P61224

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi12G → V: Constitutively activated. 1
Mutagenesisi25Q → A: Impairs interaction with KRIT1. 1
Mutagenesisi32Y → A: 25-fold reduction in RAP1GAP-stimulated GTPase activity. 1 Publication1
Mutagenesisi32Y → F: 2-fold reduction in RAP1GAP-stimulated GTPase activity. 1 Publication1
Mutagenesisi37E → A: Strong reduction in nucleotide exchange with EPAC2. 1 Publication1
Mutagenesisi38D → A: Impairs interaction with KRIT1. 1
Mutagenesisi63Q → E: Abolishes complex formation with RAP1GAP. Loss GTPase activity. 1 Publication1
Mutagenesisi64F → A: Abolishes complex formation with RAP1GAP. Loss GTPase activity. 1 Publication1
Mutagenesisi179S → A: Abolishes phosphorylation by PKA. 1 Publication1

Organism-specific databases

DisGeNETi5908
MalaCardsiRAP1B
OpenTargetsiENSG00000127314
PharmGKBiPA34219

Polymorphism and mutation databases

BioMutaiRAP1B
DMDMi47117723

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000302091 – 181Ras-related protein Rap-1bAdd BLAST181
PropeptideiPRO_0000030210182 – 184Removed in mature form3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei39ADP-ribosylserine; by botulinum toxin1 Publication1
Modified residuei179Phosphoserine; by PKA1 Publication1
Modified residuei181Cysteine methyl ester1 Publication1
Lipidationi181S-geranylgeranyl cysteine1 Publication1

Keywords - PTMi

ADP-ribosylation, Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiP61224
MaxQBiP61224
PaxDbiP61224
PeptideAtlasiP61224
PRIDEiP61224
ProteomicsDBi57277
TopDownProteomicsiP61224-1 [P61224-1]
P61224-2 [P61224-2]

2D gel databases

OGPiP09526

PTM databases

iPTMnetiP61224
PhosphoSitePlusiP61224
SwissPalmiP61224

Expressioni

Gene expression databases

BgeeiENSG00000127314 Expressed in 94 organ(s), highest expression level in leukocyte
CleanExiHS_RAP1B
ExpressionAtlasiP61224 baseline and differential
GenevisibleiP61224 HS

Interactioni

Subunit structurei

Heterodimer with RAP1GAP. Interacts with EPAC2, SGSM1, SGSM2 and SGSM3. Interacts with KRIT1.4 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi111843, 76 interactors
DIPiDIP-35407N
IntActiP61224, 53 interactors
MINTiP61224
STRINGi9606.ENSP00000250559

Structurei

Secondary structure

1184
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP61224
SMRiP61224
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61224

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni25 – 67Interaction with KRIT11 PublicationAdd BLAST43

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector regionCurated9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00860000133678
HOGENOMiHOG000233973
HOVERGENiHBG009351
InParanoidiP61224
KOiK07836
OMAiWGNAPYY
OrthoDBiEOG091G0OOG
PhylomeDBiP61224
TreeFamiTF313014

Family and domain databases

CDDicd04175 Rap1, 1 hit
InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR038851 Rap1
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras-type
PANTHERiPTHR24070 PTHR24070, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 18 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P61224-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDAQQ
60 70 80 90 100
CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI
110 120 130 140 150
LRVKDTDDVP MILVGNKCDL EDERVVGKEQ GQNLARQWNN CAFLESSAKS
160 170 180
KINVNEIFYD LVRQINRKTP VPGKARKKSS CQLL
Length:184
Mass (Da):20,825
Last modified:May 10, 2004 - v1
Checksum:iCE976895E5965224
GO
Isoform 2 (identifier: P61224-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     62-108: Missing.

Show »
Length:137
Mass (Da):15,356
Checksum:iA142020053FE1BC5
GO
Isoform 3 (identifier: P61224-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     43-61: Missing.

Show »
Length:165
Mass (Da):18,778
Checksum:i5F70CFF192C43A4A
GO
Isoform 4 (identifier: P61224-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     20-61: Missing.

Show »
Length:142
Mass (Da):16,033
Checksum:iEA48A041F34E0074
GO

Computationally mapped potential isoform sequencesi

There are 18 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E7ESV4E7ESV4_HUMAN
Ras-related protein Rap-1b
RAP1B
157Annotation score:
F5H7Y6F5H7Y6_HUMAN
Ras-related protein Rap-1b
RAP1B
179Annotation score:
B7ZB78B7ZB78_HUMAN
cDNA, FLJ79442, highly similar to R...
RAP1B
118Annotation score:
F5H500F5H500_HUMAN
Ras-related protein Rap-1b
RAP1B
101Annotation score:
F5GX62F5GX62_HUMAN
Ras-related protein Rap-1b
RAP1B
139Annotation score:
F5H491F5H491_HUMAN
Ras-related protein Rap-1b
RAP1B
93Annotation score:
F5H004F5H004_HUMAN
Ras-related protein Rap-1b
RAP1B
127Annotation score:
F5GZG1F5GZG1_HUMAN
Ras-related protein Rap-1b
RAP1B
121Annotation score:
F5H0B7F5H0B7_HUMAN
Ras-related protein Rap-1b
RAP1B
98Annotation score:
F5H823F5H823_HUMAN
Ras-related protein Rap-1b
RAP1B
103Annotation score:
There are more potential isoformsShow all

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8V → F in AAH95467 (PubMed:15489334).Curated1
Sequence conflicti16K → N in AAH78173 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04530320 – 61Missing in isoform 4. 1 PublicationAdd BLAST42
Alternative sequenceiVSP_04530443 – 61Missing in isoform 3. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_04530562 – 108Missing in isoform 2. 1 PublicationAdd BLAST47

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X08004 mRNA Translation: CAB46488.1
AL080212 mRNA Translation: CAB45777.1
AB062128 mRNA Translation: BAB93460.1
AF493913 mRNA Translation: AAM12627.1
AK298818 mRNA Translation: BAG60950.1
AK301401 mRNA Translation: BAG62936.1
AK301428 mRNA Translation: BAG62956.1
AK312371 mRNA Translation: BAG35289.1
CR407689 mRNA Translation: CAG28617.1
BT020093 mRNA Translation: AAV38896.1
EF581377 Genomic DNA Translation: ABQ52130.1
AC015550 Genomic DNA No translation available.
CH471054 Genomic DNA Translation: EAW97189.1
BC000176 mRNA Translation: AAH00176.1
BC078173 mRNA Translation: AAH78173.1
BC095467 mRNA Translation: AAH95467.1
CCDSiCCDS58252.1 [P61224-2]
CCDS58253.1 [P61224-3]
CCDS58254.1 [P61224-4]
CCDS8984.1 [P61224-1]
PIRiS01952 TVHUR1
RefSeqiNP_001010942.1, NM_001010942.2 [P61224-1]
NP_001238846.1, NM_001251917.1 [P61224-4]
NP_001238847.1, NM_001251918.1 [P61224-4]
NP_001238850.1, NM_001251921.1 [P61224-3]
NP_001238851.1, NM_001251922.1 [P61224-2]
NP_056461.1, NM_015646.5 [P61224-1]
UniGeneiHs.369920

Genome annotation databases

EnsembliENST00000250559; ENSP00000250559; ENSG00000127314 [P61224-1]
ENST00000341355; ENSP00000441275; ENSG00000127314 [P61224-1]
ENST00000393436; ENSP00000377085; ENSG00000127314 [P61224-1]
ENST00000450214; ENSP00000399986; ENSG00000127314 [P61224-4]
ENST00000537460; ENSP00000439966; ENSG00000127314 [P61224-1]
ENST00000539091; ENSP00000444830; ENSG00000127314 [P61224-4]
ENST00000540209; ENSP00000446318; ENSG00000127314 [P61224-3]
ENST00000542145; ENSP00000440014; ENSG00000127314 [P61224-2]
GeneIDi5908
KEGGihsa:5908
UCSCiuc001sub.5 human [P61224-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology
SeattleSNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X08004 mRNA Translation: CAB46488.1
AL080212 mRNA Translation: CAB45777.1
AB062128 mRNA Translation: BAB93460.1
AF493913 mRNA Translation: AAM12627.1
AK298818 mRNA Translation: BAG60950.1
AK301401 mRNA Translation: BAG62936.1
AK301428 mRNA Translation: BAG62956.1
AK312371 mRNA Translation: BAG35289.1
CR407689 mRNA Translation: CAG28617.1
BT020093 mRNA Translation: AAV38896.1
EF581377 Genomic DNA Translation: ABQ52130.1
AC015550 Genomic DNA No translation available.
CH471054 Genomic DNA Translation: EAW97189.1
BC000176 mRNA Translation: AAH00176.1
BC078173 mRNA Translation: AAH78173.1
BC095467 mRNA Translation: AAH95467.1
CCDSiCCDS58252.1 [P61224-2]
CCDS58253.1 [P61224-3]
CCDS58254.1 [P61224-4]
CCDS8984.1 [P61224-1]
PIRiS01952 TVHUR1
RefSeqiNP_001010942.1, NM_001010942.2 [P61224-1]
NP_001238846.1, NM_001251917.1 [P61224-4]
NP_001238847.1, NM_001251918.1 [P61224-4]
NP_001238850.1, NM_001251921.1 [P61224-3]
NP_001238851.1, NM_001251922.1 [P61224-2]
NP_056461.1, NM_015646.5 [P61224-1]
UniGeneiHs.369920

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BRWX-ray3.40D1-167[»]
3CF6X-ray2.20R1-167[»]
4DXAX-ray1.95A1-167[»]
4HDOX-ray1.67B1-167[»]
4HDQX-ray1.95B1-167[»]
4M8NX-ray3.29E/F/G/H1-166[»]
4MGIX-ray2.80R1-167[»]
4MGKX-ray2.70R1-167[»]
4MGYX-ray2.60R1-167[»]
4MGZX-ray3.00R1-167[»]
4MH0X-ray2.40R1-167[»]
5KHOX-ray2.78C/D1-167[»]
6AXFX-ray3.10B/D/F/H/J/L/N/P1-167[»]
6BA6NMR-B1-167[»]
ProteinModelPortaliP61224
SMRiP61224
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111843, 76 interactors
DIPiDIP-35407N
IntActiP61224, 53 interactors
MINTiP61224
STRINGi9606.ENSP00000250559

PTM databases

iPTMnetiP61224
PhosphoSitePlusiP61224
SwissPalmiP61224

Polymorphism and mutation databases

BioMutaiRAP1B
DMDMi47117723

2D gel databases

OGPiP09526

Proteomic databases

EPDiP61224
MaxQBiP61224
PaxDbiP61224
PeptideAtlasiP61224
PRIDEiP61224
ProteomicsDBi57277
TopDownProteomicsiP61224-1 [P61224-1]
P61224-2 [P61224-2]

Protocols and materials databases

DNASUi5908
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000250559; ENSP00000250559; ENSG00000127314 [P61224-1]
ENST00000341355; ENSP00000441275; ENSG00000127314 [P61224-1]
ENST00000393436; ENSP00000377085; ENSG00000127314 [P61224-1]
ENST00000450214; ENSP00000399986; ENSG00000127314 [P61224-4]
ENST00000537460; ENSP00000439966; ENSG00000127314 [P61224-1]
ENST00000539091; ENSP00000444830; ENSG00000127314 [P61224-4]
ENST00000540209; ENSP00000446318; ENSG00000127314 [P61224-3]
ENST00000542145; ENSP00000440014; ENSG00000127314 [P61224-2]
GeneIDi5908
KEGGihsa:5908
UCSCiuc001sub.5 human [P61224-1]

Organism-specific databases

CTDi5908
DisGeNETi5908
EuPathDBiHostDB:ENSG00000127314.17
GeneCardsiRAP1B
H-InvDBiHIX0032084
HGNCiHGNC:9857 RAP1B
MalaCardsiRAP1B
MIMi179530 gene
neXtProtiNX_P61224
OpenTargetsiENSG00000127314
PharmGKBiPA34219
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0395 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00860000133678
HOGENOMiHOG000233973
HOVERGENiHBG009351
InParanoidiP61224
KOiK07836
OMAiWGNAPYY
OrthoDBiEOG091G0OOG
PhylomeDBiP61224
TreeFamiTF313014

Enzyme and pathway databases

ReactomeiR-HSA-354192 Integrin alphaIIb beta3 signaling
R-HSA-354194 GRB2:SOS provides linkage to MAPK signaling for Integrins
R-HSA-372708 p130Cas linkage to MAPK signaling for integrins
R-HSA-392517 Rap1 signalling
R-HSA-5674135 MAP2K and MAPK activation
R-HSA-6798695 Neutrophil degranulation
R-HSA-6802946 Signaling by moderate kinase activity BRAF mutants
R-HSA-6802948 Signaling by high-kinase activity BRAF mutants
R-HSA-6802949 Signaling by RAS mutants
R-HSA-6802952 Signaling by BRAF and RAF fusions
R-HSA-6802955 Paradoxical activation of RAF signaling by kinase inactive BRAF
R-HSA-8875555 MET activates RAP1 and RAC1
R-HSA-8950505 Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation
SignaLinkiP61224
SIGNORiP61224

Miscellaneous databases

ChiTaRSiRAP1B human
EvolutionaryTraceiP61224
GeneWikiiRAP1B
GenomeRNAii5908
PROiPR:P61224
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000127314 Expressed in 94 organ(s), highest expression level in leukocyte
CleanExiHS_RAP1B
ExpressionAtlasiP61224 baseline and differential
GenevisibleiP61224 HS

Family and domain databases

CDDicd04175 Rap1, 1 hit
InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR038851 Rap1
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras-type
PANTHERiPTHR24070 PTHR24070, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAP1B_HUMAN
AccessioniPrimary (citable) accession number: P61224
Secondary accession number(s): B2R5Z2
, B4DQI8, B4DW74, B4DW94, P09526, Q502X3, Q5TZR4, Q6DCA1, Q6LES0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: September 12, 2018
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again