UniProtKB - P61222 (ABCE1_MOUSE)
Protein
ATP-binding cassette sub-family E member 1
Gene
Abce1
Organism
Mus musculus (Mouse)
Status
Functioni
Cotranslational quality control factor involved in the No-Go Decay (NGD) pathway (By similarity). Together with PELO and HBS1L, is required for 48S complex formation from 80S ribosomes and dissociation of vacant 80S ribosomes (By similarity). Together with PELO and HBS1L, recognizes stalled ribosomes and promotes dissociation of elongation complexes assembled on non-stop mRNAs; this triggers endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and to degrade damaged mRNAs as part of the No-Go Decay (NGD) pathway (By similarity). Plays a role in the regulation of mRNA turnover (PubMed:10866653). Plays a role in quality control of translation of mitochondrial outer membrane-localized mRNA (By similarity). As part of the PINK1-regulated signaling, ubiquitinated by CNOT4 upon mitochondria damage; this modification generates polyubiquitin signals that recruit autophagy receptors to the mitochondrial outer membrane and initiate mitophagy (By similarity). RNASEL-specific protein inhibitor which antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) to RNASEL (By similarity). Negative regulator of the anti-viral effect of the interferon-regulated 2-5A/RNASEL pathway (By similarity).By similarity1 Publication
Miscellaneous
The ABC transporter domains seem not to be functional.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 110 – 117 | ATP 1PROSITE-ProRule annotation | 8 | |
Nucleotide bindingi | 379 – 386 | ATP 2PROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- ATPase activity Source: InterPro
- ATP binding Source: GO_Central
- endoribonuclease inhibitor activity Source: MGI
- iron ion binding Source: GO_Central
- ribosomal small subunit binding Source: GO_Central
GO - Biological processi
- negative regulation of endoribonuclease activity Source: MGI
- regulation of translation Source: UniProtKB-KW
- ribosomal subunit export from nucleus Source: GO_Central
- translational initiation Source: GO_Central
- translational termination Source: GO_Central
Keywordsi
Molecular function | Chaperone |
Biological process | Translation regulation |
Ligand | 4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-8983711, OAS antiviral response R-MMU-909733, Interferon alpha/beta signaling |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-binding cassette sub-family E member 1Alternative name(s): RNase L inhibitor Ribonuclease 4 inhibitor Short name: RNS4I |
Gene namesi | Name:Abce1 Synonyms:Rli |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1195458, Abce1 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Mitochondrion
- Mitochondrion By similarity
Note: Localized to clusters of virus formation at the plasma membrane.By similarity
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrion Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093317 | 1 – 599 | ATP-binding cassette sub-family E member 1Add BLAST | 599 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 417 | PhosphoserineBy similarity | 1 | |
Modified residuei | 550 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Ubiquitinated by CNOT4 (By similarity). Ubiquitination mediates the recruitment of autophagy receptors to the mitochondrial outer membrane and initiates mitophagy (By similarity).By similarity
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P61222 |
jPOSTi | P61222 |
PaxDbi | P61222 |
PeptideAtlasi | P61222 |
PRIDEi | P61222 |
ProteomicsDBi | 286047 |
PTM databases
iPTMneti | P61222 |
PhosphoSitePlusi | P61222 |
SwissPalmi | P61222 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000058355, Expressed in cumulus cell and 321 other tissues |
ExpressionAtlasi | P61222, baseline and differential |
Genevisiblei | P61222, MM |
Interactioni
Subunit structurei
Interacts with PINK1, CNOT4 and PELO. Probably heterodimerizes with RNASEL; this interaction inhibits RNASEL.
By similarityProtein-protein interaction databases
BioGRIDi | 204855, 42 interactors |
IntActi | P61222, 2 interactors |
MINTi | P61222 |
STRINGi | 10090.ENSMUSP00000079379 |
Miscellaneous databases
RNActi | P61222, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 7 – 37 | 4Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST | 31 | |
Domaini | 46 – 75 | 4Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST | 30 | |
Domaini | 79 – 315 | ABC transporter 1PROSITE-ProRule annotationAdd BLAST | 237 | |
Domaini | 342 – 562 | ABC transporter 2PROSITE-ProRule annotationAdd BLAST | 221 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0063, Eukaryota |
GeneTreei | ENSGT00390000015089 |
HOGENOMi | CLU_017344_4_1_1 |
InParanoidi | P61222 |
OMAi | MVCIQNG |
OrthoDBi | 359213at2759 |
PhylomeDBi | P61222 |
TreeFami | TF105206 |
Family and domain databases
CDDi | cd03236, ABC_RNaseL_inhibitor_domain1, 1 hit |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR003593, AAA+_ATPase IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR027417, P-loop_NTPase IPR013283, RLI1 IPR034348, RLI_dom_1 IPR007209, RNaseL-inhib_metal-bd_dom |
PANTHERi | PTHR19248, PTHR19248, 1 hit |
Pfami | View protein in Pfam PF00005, ABC_tran, 2 hits PF00037, Fer4, 1 hit PF04068, RLI, 1 hit |
PRINTSi | PR01868, ABCEFAMILY |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 1 hit PS51379, 4FE4S_FER_2, 2 hits PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 2 hits |
i Sequence
Sequence statusi: Complete.
P61222-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADKLTRIAI VNHDKCKPKK CRQECKKSCP VVRMGKLCIE VTPQSKIAWI
60 70 80 90 100
SETLCIGCGI CIKKCPFGAL SIVNLPSNLE KETTHRYCAN AFKLHRLPIP
110 120 130 140 150
RPGEVLGLVG TNGIGKSTAL KILAGKQKPN LGKYDDPPDW QEILTYFRGS
160 170 180 190 200
ELQNYFTKIL EDDLKAIIKP QYVDQIPKAA KGTVGSILDR KDETKTQAIV
210 220 230 240 250
CQQLDLTHLK ERNVEDLSGG ELQRFACAVV CIQKADIFMF DEPSSYLDVK
260 270 280 290 300
QRLKAAITIR SLINPDRYII VVEHDLSVLD YLSDFICCLY GVPSAYGVVT
310 320 330 340 350
MPFSVREGIN IFLDGYVPTE NLRFRDASLV FKVAETANEE EVKKMCMYKY
360 370 380 390 400
PGMKKKMGEF ELAIVAGEFT DSEIMVMLGE NGTGKTTFIR MLAGRLKPDE
410 420 430 440 450
GGEVPVLNVS YKPQKISPKS TGSVRQLLHE KIRDAYTHPQ FVTDVMKPLQ
460 470 480 490 500
IENIIDQEVQ TLSGGELQRV ALALCLGKPA DVYLIDEPSA YLDSEQRLMA
510 520 530 540 550
ARVVKRFILH AKKTAFVVEH DFIMATYLAD RVIVFDGVPS KNTVANSPQT
560 570 580 590
LLAGMNKFLS QLEITFRRDP NNYRPRINKL NSIKDVEQKK SGNYFFLDD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 153 | Q → K in AAC24730 (PubMed:9524254).Curated | 1 | |
Sequence conflicti | 158 | K → T in AAC24730 (PubMed:9524254).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U90446 mRNA Translation: AAC24730.1 BC005422 mRNA Translation: AAH05422.1 |
CCDSi | CCDS22438.1 |
PIRi | JC6555 |
RefSeqi | NP_056566.2, NM_015751.2 |
Genome annotation databases
Ensembli | ENSMUST00000080536; ENSMUSP00000079379; ENSMUSG00000058355 |
GeneIDi | 24015 |
KEGGi | mmu:24015 |
UCSCi | uc009mit.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U90446 mRNA Translation: AAC24730.1 BC005422 mRNA Translation: AAH05422.1 |
CCDSi | CCDS22438.1 |
PIRi | JC6555 |
RefSeqi | NP_056566.2, NM_015751.2 |
3D structure databases
SMRi | P61222 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204855, 42 interactors |
IntActi | P61222, 2 interactors |
MINTi | P61222 |
STRINGi | 10090.ENSMUSP00000079379 |
PTM databases
iPTMneti | P61222 |
PhosphoSitePlusi | P61222 |
SwissPalmi | P61222 |
Proteomic databases
EPDi | P61222 |
jPOSTi | P61222 |
PaxDbi | P61222 |
PeptideAtlasi | P61222 |
PRIDEi | P61222 |
ProteomicsDBi | 286047 |
Protocols and materials databases
Antibodypediai | 27438, 315 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000080536; ENSMUSP00000079379; ENSMUSG00000058355 |
GeneIDi | 24015 |
KEGGi | mmu:24015 |
UCSCi | uc009mit.1, mouse |
Organism-specific databases
CTDi | 6059 |
MGIi | MGI:1195458, Abce1 |
Phylogenomic databases
eggNOGi | KOG0063, Eukaryota |
GeneTreei | ENSGT00390000015089 |
HOGENOMi | CLU_017344_4_1_1 |
InParanoidi | P61222 |
OMAi | MVCIQNG |
OrthoDBi | 359213at2759 |
PhylomeDBi | P61222 |
TreeFami | TF105206 |
Enzyme and pathway databases
Reactomei | R-MMU-8983711, OAS antiviral response R-MMU-909733, Interferon alpha/beta signaling |
Miscellaneous databases
BioGRID-ORCSi | 24015, 20 hits in 51 CRISPR screens |
ChiTaRSi | Abce1, mouse |
PROi | PR:P61222 |
RNActi | P61222, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000058355, Expressed in cumulus cell and 321 other tissues |
ExpressionAtlasi | P61222, baseline and differential |
Genevisiblei | P61222, MM |
Family and domain databases
CDDi | cd03236, ABC_RNaseL_inhibitor_domain1, 1 hit |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR003593, AAA+_ATPase IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR027417, P-loop_NTPase IPR013283, RLI1 IPR034348, RLI_dom_1 IPR007209, RNaseL-inhib_metal-bd_dom |
PANTHERi | PTHR19248, PTHR19248, 1 hit |
Pfami | View protein in Pfam PF00005, ABC_tran, 2 hits PF00037, Fer4, 1 hit PF04068, RLI, 1 hit |
PRINTSi | PR01868, ABCEFAMILY |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 1 hit PS51379, 4FE4S_FER_2, 2 hits PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ABCE1_MOUSE | |
Accessioni | P61222Primary (citable) accession number: P61222 Secondary accession number(s): O88793 Q99K66 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2004 |
Last sequence update: | May 10, 2004 | |
Last modified: | April 7, 2021 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families