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Protein

DNA-directed RNA polymerases I, II, and III subunit RPABC2

Gene

POLR2F

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-1834949 Cytosolic sensors of pathogen-associated DNA
R-HSA-203927 MicroRNA (miRNA) biogenesis
R-HSA-427413 NoRC negatively regulates rRNA expression
R-HSA-452723 Transcriptional regulation of pluripotent stem cells
R-HSA-5250924 B-WICH complex positively regulates rRNA expression
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-5601884 PIWI-interacting RNA (piRNA) biogenesis
R-HSA-5617472 Activation of anterior HOX genes in hindbrain development during early embryogenesis
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6781823 Formation of TC-NER Pre-Incision Complex
R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6803529 FGFR2 alternative splicing
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-72086 mRNA Capping
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72165 mRNA Splicing - Minor Pathway
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-73762 RNA Polymerase I Transcription Initiation
R-HSA-73772 RNA Polymerase I Promoter Escape
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73777 RNA Polymerase I Chain Elongation
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-73780 RNA Polymerase III Chain Elongation
R-HSA-73863 RNA Polymerase I Transcription Termination
R-HSA-73980 RNA Polymerase III Transcription Termination
R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-76061 RNA Polymerase III Transcription Initiation From Type 1 Promoter
R-HSA-76066 RNA Polymerase III Transcription Initiation From Type 2 Promoter
R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-HSA-8851708 Signaling by FGFR2 IIIa TM
R-HSA-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerases I, II, and III subunit RPABC2
Short name:
RNA polymerases I, II, and III subunit ABC2
Alternative name(s):
DNA-directed RNA polymerase II subunit F
DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide
RPABC14.4
Short name:
RPB14.4
RPB6 homolog
RPC15
Gene namesi
Name:POLR2F
Synonyms:POLRF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000100142.14
HGNCiHGNC:9193 POLR2F
MIMi604414 gene
neXtProtiNX_P61218

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5435
OpenTargetsiENSG00000100142
PharmGKBiPA33513

Polymorphism and mutation databases

BioMutaiPOLR2F
DMDMi47117761

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001337992 – 127DNA-directed RNA polymerases I, II, and III subunit RPABC2Add BLAST126

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2Phosphoserine; by CK2By similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP61218
MaxQBiP61218
PaxDbiP61218
PeptideAtlasiP61218
PRIDEiP61218
ProteomicsDBi57275

PTM databases

iPTMnetiP61218
PhosphoSitePlusiP61218

Expressioni

Gene expression databases

BgeeiENSG00000100142 Expressed in 136 organ(s), highest expression level in C1 segment of cervical spinal cord
CleanExiHS_POLR2F
ExpressionAtlasiP61218 baseline and differential
GenevisibleiP61218 HS

Organism-specific databases

HPAiCAB009882
HPA000827

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively.By similarity

Protein-protein interaction databases

BioGridi111431, 76 interactors
CORUMiP61218
DIPiDIP-32915N
IntActiP61218, 63 interactors
STRINGi9606.ENSP00000403852

Structurei

Secondary structure

1127
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP61218
SMRiP61218
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61218

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3405 Eukaryota
COG1758 LUCA
GeneTreeiENSGT00390000010415
HOGENOMiHOG000225272
HOVERGENiHBG002375
InParanoidiP61218
KOiK03014
OMAiCDELIIS
PhylomeDBiP61218
TreeFamiTF103041

Family and domain databases

Gene3Di3.90.940.10, 1 hit
InterProiView protein in InterPro
IPR020708 DNA-dir_RNA_polK_14-18kDa_CS
IPR006110 Pol_omega/K/RPB6
IPR012293 RNAP_RPB6_omega
IPR028363 RPB6
IPR036161 RPB6/omega_like_sf
IPR006111 RpoK/Rpb6
PfamiView protein in Pfam
PF01192 RNA_pol_Rpb6, 1 hit
PIRSFiPIRSF500154 RPB6, 1 hit
PIRSF000778 RpoK/RPB6, 1 hit
SMARTiView protein in SMART
SM01409 RNA_pol_Rpb6, 1 hit
SUPFAMiSSF63562 SSF63562, 1 hit
PROSITEiView protein in PROSITE
PS01111 RNA_POL_K_14KD, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 10 potential isoforms that are computationally mapped.iShow all

P61218-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSDNEDNFDG DDFDDVEEDE GLDDLENAEE EGQENVEILP SGERPQANQK
60 70 80 90 100
RITTPYMTKY ERARVLGTRA LQIAMCAPVM VELEGETDPL LIAMKELKAR
110 120
KIPIIIRRYL PDGSYEDWGV DELIITD
Length:127
Mass (Da):14,478
Last modified:May 10, 2004 - v1
Checksum:i6362B0D7EB3F0921
GO

Computationally mapped potential isoform sequencesi

There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
U3KQB0U3KQB0_HUMAN
DNA-directed RNA polymerases I, II,...
POLR2F
122Annotation score:
U3KPY1U3KPY1_HUMAN
DNA-directed RNA polymerases I, II,...
POLR2F
112Annotation score:
B0QYL9B0QYL9_HUMAN
DNA-directed RNA polymerases I, II,...
POLR2F
158Annotation score:
F8WC47F8WC47_HUMAN
DNA-directed RNA polymerases I, II,...
POLR2F
111Annotation score:
B0QYL8B0QYL8_HUMAN
DNA-directed RNA polymerases I, II,...
POLR2F
126Annotation score:
U3KQS8U3KQS8_HUMAN
DNA-directed RNA polymerases I, II,...
POLR2F
98Annotation score:
U3KPV3U3KPV3_HUMAN
DNA-directed RNA polymerases I, II,...
POLR2F
74Annotation score:
U3KQQ7U3KQQ7_HUMAN
DNA-directed RNA polymerases I, II,...
POLR2F
76Annotation score:
H0Y2U7H0Y2U7_HUMAN
DNA-directed RNA polymerases I, II,...
POLR2F
145Annotation score:
H0Y439H0Y439_HUMAN
DNA-directed RNA polymerases I, II,...
POLR2F
93Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03657160Y → N in a breast cancer sample; somatic mutation. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z27113 Genomic DNA Translation: CAA81629.1
AF006501 Genomic DNA No translation available.
CR456546 mRNA Translation: CAG30432.1
CR457021 mRNA Translation: CAG33302.1
AL031587 Genomic DNA No translation available.
CH471095 Genomic DNA Translation: EAW60203.1
BC003582 mRNA Translation: AAH03582.1
CCDSiCCDS13963.1
PIRiI38175 S38627
RefSeqiNP_068809.1, NM_021974.4
UniGeneiHs.436578
Hs.735742

Genome annotation databases

EnsembliENST00000442738; ENSP00000403852; ENSG00000100142
GeneIDi5435
KEGGihsa:5435
UCSCiuc003aul.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiRPAB2_HUMAN
AccessioniPrimary (citable) accession number: P61218
Secondary accession number(s): P41584, Q6IAY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: September 12, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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