UniProtKB - P61204 (ARF3_HUMAN)
Protein
ADP-ribosylation factor 3
Gene
ARF3
Organism
Homo sapiens (Human)
Status
Functioni
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 24 – 31 | GTPBy similarity | 8 | |
Nucleotide bindingi | 67 – 71 | GTPBy similarity | 5 | |
Nucleotide bindingi | 126 – 129 | GTPBy similarity | 4 |
GO - Molecular functioni
- GTPase activity Source: ProtInc
- GTP binding Source: GO_Central
GO - Biological processi
- intracellular protein transport Source: GO_Central
- phosphatidylinositol biosynthetic process Source: Reactome
- retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: WormBase
- vesicle-mediated transport Source: GO_Central
Keywordsi
Biological process | ER-Golgi transport, Protein transport, Transport |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P61204 |
Reactomei | R-HSA-1660514, Synthesis of PIPs at the Golgi membrane R-HSA-6807878, COPI-mediated anterograde transport R-HSA-6811434, COPI-dependent Golgi-to-ER retrograde traffic |
Names & Taxonomyi
Protein namesi | Recommended name: ADP-ribosylation factor 3 |
Gene namesi | Name:ARF3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:654, ARF3 |
MIMi | 103190, gene |
neXtProti | NX_P61204 |
VEuPathDBi | HostDB:ENSG00000134287.9 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus 1 Publication
Other locations
- perinuclear region 1 Publication
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Golgi apparatus
- Golgi membrane Source: Reactome
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, Golgi apparatusPathology & Biotechi
Organism-specific databases
DisGeNETi | 377 |
OpenTargetsi | ENSG00000134287 |
PharmGKBi | PA24936 |
Miscellaneous databases
Pharosi | P61204, Tbio |
Genetic variation databases
BioMutai | ARF3 |
DMDMi | 47117657 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedSequence analysis | |||
ChainiPRO_0000207386 | 2 – 181 | ADP-ribosylation factor 3Add BLAST | 180 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineSequence analysis | 1 |
Keywords - PTMi
Lipoprotein, MyristateProteomic databases
EPDi | P61204 |
jPOSTi | P61204 |
MassIVEi | P61204 |
MaxQBi | P61204 |
PaxDbi | P61204 |
PeptideAtlasi | P61204 |
PRIDEi | P61204 |
ProteomicsDBi | 57274 [P61204-1] 7097 |
TopDownProteomicsi | P61204-1 [P61204-1] |
2D gel databases
OGPi | P16587 |
PTM databases
iPTMneti | P61204 |
MetOSitei | P61204 |
PhosphoSitePlusi | P61204 |
SwissPalmi | P61204 |
Expressioni
Gene expression databases
Bgeei | ENSG00000134287, Expressed in prefrontal cortex and 241 other tissues |
ExpressionAtlasi | P61204, baseline and differential |
Genevisiblei | P61204, HS |
Organism-specific databases
HPAi | ENSG00000134287, Tissue enhanced (brain) |
Interactioni
Subunit structurei
Interacts with PRKCABP.
Interacts with PI4KB and NCS1/FREQ at the Golgi complex.
2 PublicationsBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 106872, 43 interactors |
IntActi | P61204, 14 interactors |
MINTi | P61204 |
STRINGi | 9606.ENSP00000256682 |
Miscellaneous databases
RNActi | P61204, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P61204 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0070, Eukaryota |
GeneTreei | ENSGT00950000183080 |
HOGENOMi | CLU_040729_9_3_1 |
InParanoidi | P61204 |
OMAi | CHAQGEM |
OrthoDBi | 1362554at2759 |
PhylomeDBi | P61204 |
TreeFami | TF300808 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR006689, Small_GTPase_ARF/SAR |
Pfami | View protein in Pfam PF00025, Arf, 1 hit |
PRINTSi | PR00328, SAR1GTPBP |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51417, ARF, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P61204-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGNIFGNLLK SLIGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV
110 120 130 140 150
NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH
160 170 180
RNWYIQATCA TSGDGLYEGL DWLANQLKNK K
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF5H0C7 | F5H0C7_HUMAN | ADP-ribosylation factor 3 | ARF3 | 102 | Annotation score: | ||
F5H6T5 | F5H6T5_HUMAN | ADP-ribosylation factor 3 | ARF3 | 50 | Annotation score: | ||
F5H1V1 | F5H1V1_HUMAN | ADP-ribosylation factor 3 | ARF3 | 40 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056935 | 50 – 86 | Missing in isoform 2. 1 PublicationAdd BLAST | 37 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74493 Genomic DNA Translation: AAA58359.1 M33384 mRNA Translation: AAA83931.1 M74491 mRNA Translation: AAB59425.1 AF493882 mRNA Translation: AAM12596.1 BT006670 mRNA Translation: AAP35316.1 AK291633 mRNA Translation: BAF84322.1 AK316528 mRNA Translation: BAH14899.1 AC073610 Genomic DNA No translation available. CH471111 Genomic DNA Translation: EAW58026.1 BC007647 mRNA Translation: AAH07647.1 BC007762 mRNA Translation: AAH07762.1 BC017565 mRNA Translation: AAH17565.1 BC028402 mRNA Translation: AAH28402.1 |
CCDSi | CCDS8774.1 [P61204-1] |
PIRi | A41570 |
RefSeqi | NP_001650.1, NM_001659.2 [P61204-1] XP_005268913.1, XM_005268856.1 [P61204-1] |
Genome annotation databases
Ensembli | ENST00000256682; ENSP00000256682; ENSG00000134287 [P61204-1] ENST00000447318; ENSP00000395370; ENSG00000134287 [P61204-2] ENST00000541959; ENSP00000438510; ENSG00000134287 [P61204-1] |
GeneIDi | 377 |
KEGGi | hsa:377 |
UCSCi | uc001rsr.3, human [P61204-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74493 Genomic DNA Translation: AAA58359.1 M33384 mRNA Translation: AAA83931.1 M74491 mRNA Translation: AAB59425.1 AF493882 mRNA Translation: AAM12596.1 BT006670 mRNA Translation: AAP35316.1 AK291633 mRNA Translation: BAF84322.1 AK316528 mRNA Translation: BAH14899.1 AC073610 Genomic DNA No translation available. CH471111 Genomic DNA Translation: EAW58026.1 BC007647 mRNA Translation: AAH07647.1 BC007762 mRNA Translation: AAH07762.1 BC017565 mRNA Translation: AAH17565.1 BC028402 mRNA Translation: AAH28402.1 |
CCDSi | CCDS8774.1 [P61204-1] |
PIRi | A41570 |
RefSeqi | NP_001650.1, NM_001659.2 [P61204-1] XP_005268913.1, XM_005268856.1 [P61204-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6II6 | X-ray | 2.10 | C/D | 13-176 | [»] | |
SMRi | P61204 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 106872, 43 interactors |
IntActi | P61204, 14 interactors |
MINTi | P61204 |
STRINGi | 9606.ENSP00000256682 |
PTM databases
iPTMneti | P61204 |
MetOSitei | P61204 |
PhosphoSitePlusi | P61204 |
SwissPalmi | P61204 |
Genetic variation databases
BioMutai | ARF3 |
DMDMi | 47117657 |
2D gel databases
OGPi | P16587 |
Proteomic databases
EPDi | P61204 |
jPOSTi | P61204 |
MassIVEi | P61204 |
MaxQBi | P61204 |
PaxDbi | P61204 |
PeptideAtlasi | P61204 |
PRIDEi | P61204 |
ProteomicsDBi | 57274 [P61204-1] 7097 |
TopDownProteomicsi | P61204-1 [P61204-1] |
Protocols and materials databases
Antibodypediai | 25743, 112 antibodies |
DNASUi | 377 |
Genome annotation databases
Ensembli | ENST00000256682; ENSP00000256682; ENSG00000134287 [P61204-1] ENST00000447318; ENSP00000395370; ENSG00000134287 [P61204-2] ENST00000541959; ENSP00000438510; ENSG00000134287 [P61204-1] |
GeneIDi | 377 |
KEGGi | hsa:377 |
UCSCi | uc001rsr.3, human [P61204-1] |
Organism-specific databases
CTDi | 377 |
DisGeNETi | 377 |
GeneCardsi | ARF3 |
HGNCi | HGNC:654, ARF3 |
HPAi | ENSG00000134287, Tissue enhanced (brain) |
MIMi | 103190, gene |
neXtProti | NX_P61204 |
OpenTargetsi | ENSG00000134287 |
PharmGKBi | PA24936 |
VEuPathDBi | HostDB:ENSG00000134287.9 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0070, Eukaryota |
GeneTreei | ENSGT00950000183080 |
HOGENOMi | CLU_040729_9_3_1 |
InParanoidi | P61204 |
OMAi | CHAQGEM |
OrthoDBi | 1362554at2759 |
PhylomeDBi | P61204 |
TreeFami | TF300808 |
Enzyme and pathway databases
PathwayCommonsi | P61204 |
Reactomei | R-HSA-1660514, Synthesis of PIPs at the Golgi membrane R-HSA-6807878, COPI-mediated anterograde transport R-HSA-6811434, COPI-dependent Golgi-to-ER retrograde traffic |
Miscellaneous databases
BioGRID-ORCSi | 377, 20 hits in 870 CRISPR screens |
ChiTaRSi | ARF3, human |
GeneWikii | ARF3 |
GenomeRNAii | 377 |
Pharosi | P61204, Tbio |
PROi | PR:P61204 |
RNActi | P61204, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000134287, Expressed in prefrontal cortex and 241 other tissues |
ExpressionAtlasi | P61204, baseline and differential |
Genevisiblei | P61204, HS |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR006689, Small_GTPase_ARF/SAR |
Pfami | View protein in Pfam PF00025, Arf, 1 hit |
PRINTSi | PR00328, SAR1GTPBP |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51417, ARF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARF3_HUMAN | |
Accessioni | P61204Primary (citable) accession number: P61204 Secondary accession number(s): A8K6G8, B7ZB63, P16587 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 157 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families