UniProtKB - P61169 (DRD2_RAT)
D(2) dopamine receptor
Drd2
Functioni
Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase (PubMed:1321233).
Positively regulates postnatal regression of retinal hyaloid vessels via suppression of VEGFR2/KDR activity, downstream of OPN5 (By similarity).
By similarity1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 194 | Important for receptor activation | 1 | |
Sitei | 197 | Important for receptor activation | 1 |
GO - Molecular functioni
- dopamine binding Source: RGD
- dopamine neurotransmitter receptor activity Source: RGD
- dopamine neurotransmitter receptor activity, coupled via Gi/Go Source: BHF-UCL
- G protein-coupled receptor activity Source: RGD
- heterocyclic compound binding Source: RGD
- identical protein binding Source: RGD
- ionotropic glutamate receptor binding Source: RGD
- organic cyclic compound binding Source: RGD
- protein-containing complex binding Source: RGD
- signaling receptor binding Source: RGD
GO - Biological processi
- acid secretion Source: BHF-UCL
- activation of protein kinase activity Source: RGD
- adenohypophysis development Source: RGD
- adenylate cyclase-activating adrenergic receptor signaling pathway Source: GO_Central
- adenylate cyclase-inhibiting dopamine receptor signaling pathway Source: BHF-UCL
- adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: RGD
- adult behavior Source: RGD
- adult walking behavior Source: RGD
- arachidonic acid secretion Source: RGD
- associative learning Source: RGD
- auditory behavior Source: RGD
- autophagy Source: RGD
- axonogenesis Source: RGD
- behavioral response to cocaine Source: RGD
- behavioral response to ethanol Source: RGD
- branching morphogenesis of a nerve Source: RGD
- cellular potassium ion homeostasis Source: BHF-UCL
- cerebral cortex GABAergic interneuron migration Source: RGD
- circadian regulation of gene expression Source: RGD
- dopamine metabolic process Source: RGD
- dopamine receptor signaling pathway Source: RGD
- drinking behavior Source: RGD
- feeding behavior Source: RGD
- forebrain development Source: RGD
- G protein-coupled receptor internalization Source: RGD
- G protein-coupled receptor signaling pathway Source: RGD
- grooming behavior Source: RGD
- hyaloid vascular plexus regression Source: UniProtKB
- locomotory behavior Source: RGD
- long-term memory Source: RGD
- modulation of chemical synaptic transmission Source: RGD
- negative regulation of adenylate cyclase activity Source: GO_Central
- negative regulation of blood pressure Source: RGD
- negative regulation of cell death Source: RGD
- negative regulation of cell migration Source: RGD
- negative regulation of cell population proliferation Source: RGD
- negative regulation of circadian sleep/wake cycle, sleep Source: RGD
- negative regulation of cytosolic calcium ion concentration Source: RGD
- negative regulation of dopamine receptor signaling pathway Source: RGD
- negative regulation of dopamine secretion Source: RGD
- negative regulation of innate immune response Source: RGD
- negative regulation of insulin secretion Source: RGD
- negative regulation of protein kinase B signaling Source: RGD
- negative regulation of protein phosphorylation Source: RGD
- negative regulation of protein secretion Source: RGD
- negative regulation of synaptic transmission Source: RGD
- negative regulation of synaptic transmission, glutamatergic Source: RGD
- negative regulation of voltage-gated calcium channel activity Source: BHF-UCL
- nervous system process involved in regulation of systemic arterial blood pressure Source: RGD
- neuron-neuron synaptic transmission Source: RGD
- orbitofrontal cortex development Source: RGD
- peristalsis Source: RGD
- phospholipase C-activating dopamine receptor signaling pathway Source: RGD
- pigmentation Source: RGD
- positive regulation of cytokinesis Source: RGD
- positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway Source: GO_Central
- positive regulation of dopamine uptake involved in synaptic transmission Source: RGD
- positive regulation of ERK1 and ERK2 cascade Source: RGD
- positive regulation of glial cell-derived neurotrophic factor production Source: RGD
- positive regulation of G protein-coupled receptor signaling pathway Source: RGD
- positive regulation of growth hormone secretion Source: RGD
- positive regulation of long-term synaptic potentiation Source: RGD
- positive regulation of mitotic nuclear division Source: BHF-UCL
- positive regulation of multicellular organism growth Source: RGD
- positive regulation of neuroblast proliferation Source: RGD
- positive regulation of neurogenesis Source: RGD
- positive regulation of receptor internalization Source: RGD
- positive regulation of renal sodium excretion Source: RGD
- positive regulation of transcription by RNA polymerase II Source: RGD
- positive regulation of urine volume Source: RGD
- postsynaptic modulation of chemical synaptic transmission Source: SynGO
- prepulse inhibition Source: RGD
- protein localization Source: RGD
- regulation of dopamine secretion Source: GO_Central
- regulation of dopamine uptake involved in synaptic transmission Source: RGD
- regulation of heart rate Source: RGD
- regulation of locomotion involved in locomotory behavior Source: RGD
- regulation of long-term neuronal synaptic plasticity Source: RGD
- regulation of MAPK cascade Source: RGD
- regulation of neurotransmitter uptake Source: RGD
- regulation of phosphoprotein phosphatase activity Source: RGD
- regulation of potassium ion transport Source: BHF-UCL
- regulation of sodium ion transport Source: RGD
- regulation of synapse structural plasticity Source: RGD
- regulation of synaptic transmission, GABAergic Source: RGD
- regulation of synaptic vesicle exocytosis Source: RGD
- release of sequestered calcium ion into cytosol Source: BHF-UCL
- response to amphetamine Source: RGD
- response to axon injury Source: RGD
- response to cocaine Source: RGD
- response to ethanol Source: RGD
- response to histamine Source: RGD
- response to hypoxia Source: RGD
- response to inactivity Source: RGD
- response to iron ion Source: RGD
- response to light stimulus Source: RGD
- response to morphine Source: RGD
- response to nicotine Source: RGD
- response to toxic substance Source: RGD
- response to xenobiotic stimulus Source: BHF-UCL
- sensory perception of smell Source: RGD
- startle response Source: RGD
- striatum development Source: RGD
- synapse assembly Source: BHF-UCL
- temperature homeostasis Source: RGD
- visual learning Source: RGD
- Wnt signaling pathway Source: RGD
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-RNO-390651, Dopamine receptors |
Names & Taxonomyi
Protein namesi | Recommended name: D(2) dopamine receptorAlternative name(s): Dopamine D2 receptor |
Gene namesi | Name:Drd2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2520, Drd2 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
Golgi apparatus
- Golgi apparatus membrane By similarity; Multi-pass membrane protein Sequence analysis
Golgi apparatus
- Golgi membrane Source: UniProtKB-SubCell
Plasma Membrane
- ciliary membrane Source: RGD
- integral component of plasma membrane Source: RGD
- integral component of postsynaptic membrane Source: SynGO
- integral component of presynaptic membrane Source: SynGO
- lateral plasma membrane Source: RGD
- plasma membrane Source: RGD
Other locations
- acrosomal vesicle Source: RGD
- axon Source: RGD
- axon terminus Source: RGD
- cilium Source: RGD
- cytoplasmic vesicle Source: RGD
- dendrite Source: RGD
- dendritic spine Source: RGD
- dopaminergic synapse Source: RGD
- endocytic vesicle Source: RGD
- GABA-ergic synapse Source: SynGO
- glutamatergic synapse Source: SynGO
- membrane Source: BHF-UCL
- non-motile cilium Source: RGD
- perikaryon Source: RGD
- postsynaptic density Source: RGD
- sperm flagellum Source: RGD
- synaptic vesicle membrane Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 37 | ExtracellularBy similarityAdd BLAST | 37 | |
Transmembranei | 38 – 60 | Helical; Name=1By similarityAdd BLAST | 23 | |
Topological domaini | 61 – 70 | CytoplasmicBy similarity | 10 | |
Transmembranei | 71 – 93 | Helical; Name=2By similarityAdd BLAST | 23 | |
Topological domaini | 94 – 108 | ExtracellularBy similarityAdd BLAST | 15 | |
Transmembranei | 109 – 130 | Helical; Name=3By similarityAdd BLAST | 22 | |
Topological domaini | 131 – 151 | CytoplasmicBy similarityAdd BLAST | 21 | |
Transmembranei | 152 – 172 | Helical; Name=4By similarityAdd BLAST | 21 | |
Topological domaini | 173 – 188 | ExtracellularBy similarityAdd BLAST | 16 | |
Transmembranei | 189 – 213 | Helical; Name=5By similarityAdd BLAST | 25 | |
Topological domaini | 214 – 374 | CytoplasmicBy similarityAdd BLAST | 161 | |
Transmembranei | 375 – 396 | Helical; Name=6By similarityAdd BLAST | 22 | |
Topological domaini | 397 – 410 | ExtracellularBy similarityAdd BLAST | 14 | |
Transmembranei | 411 – 432 | Helical; Name=7By similarityAdd BLAST | 22 | |
Topological domaini | 433 – 444 | CytoplasmicBy similarityAdd BLAST | 12 |
Keywords - Cellular componenti
Cell membrane, Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 193 | S → A: Moderate decrease in ligand binding. 200-fold reduction of agonist-mediated cAMP inhibition. 1 Publication | 1 | |
Mutagenesisi | 194 | S → A: Small decrease in agonist binding. Complete loss of agonist-mediated cAMP inhibition. 1 Publication | 1 | |
Mutagenesisi | 197 | S → A: Small decrease in agonist binding. 18-fold reduction of agonist-mediated cAMP inhibition. 1 Publication | 1 | |
Mutagenesisi | 420 | S → A: Moderate decrease in ligand binding. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL339 |
DrugCentrali | P61169 |
GuidetoPHARMACOLOGYi | 215 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069390 | 1 – 444 | D(2) dopamine receptorAdd BLAST | 444 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 5 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 17 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 23 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 107 ↔ 182 | PROSITE-ProRule annotation | ||
Disulfide bondi | 400 ↔ 402 | PROSITE-ProRule annotation | ||
Lipidationi | 444 | S-palmitoyl cysteineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, PalmitatePTM databases
GlyGeni | P61169, 3 sites |
iPTMneti | P61169 |
PhosphoSitePlusi | P61169 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000008428, Expressed in brain and 7 other tissues |
Genevisiblei | P61169, RN |
Interactioni
Subunit structurei
Forms homo- and heterooligomers with DRD4 (By similarity). The interaction with DRD4 may modulate agonist-induced downstream signaling (By similarity).
Interacts with CADPS and CADPS2 (By similarity).
Interacts with GPRASP1, PPP1R9B and CLIC6 (PubMed:10391935, PubMed:14499480, PubMed:16049099).
Interacts with ARRB2 (PubMed:19332542).
Interacts with HTR2A (By similarity).
Interacts with DRD1 (By similarity).
Interacts with KCNA2 (By similarity).
By similarityBinary interactionsi
P61169
With | #Exp. | IntAct |
---|---|---|
Ppp1r9b [O35274] | 2 | EBI-80012,EBI-80022 |
GO - Molecular functioni
- identical protein binding Source: RGD
- ionotropic glutamate receptor binding Source: RGD
- signaling receptor binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 246498, 11 interactors |
CORUMi | P61169 |
DIPi | DIP-30883N |
IntActi | P61169, 5 interactors |
MINTi | P61169 |
STRINGi | 10116.ENSRNOP00000043759 |
Chemistry databases
BindingDBi | P61169 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 211 – 374 | Interaction with PPP1R9B1 PublicationAdd BLAST | 164 | |
Regioni | 282 – 329 | DisorderedSequence analysisAdd BLAST | 48 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT00940000155539 |
InParanoidi | P61169 |
OMAi | HGLHSTA |
OrthoDBi | 1021787at2759 |
PhylomeDBi | P61169 |
TreeFami | TF334382 |
Family and domain databases
InterProi | View protein in InterPro IPR001922, Dopamine_D2_rcpt IPR000929, Dopamine_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00567, DOPAMINED2R PR00242, DOPAMINER PR00237, GPCRRHODOPSN |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDPLNLSWYD DDLERQNWSR PFNGSEGKAD RPHYNYYAML LTLLIFIIVF
60 70 80 90 100
GNVLVCMAVS REKALQTTTN YLIVSLAVAD LLVATLVMPW VVYLEVVGEW
110 120 130 140 150
KFSRIHCDIF VTLDVMMCTA SILNLCAISI DRYTAVAMPM LYNTRYSSKR
160 170 180 190 200
RVTVMIAIVW VLSFTISCPL LFGLNNTDQN ECIIANPAFV VYSSIVSFYV
210 220 230 240 250
PFIVTLLVYI KIYIVLRKRR KRVNTKRSSR AFRANLKTPL KGNCTHPEDM
260 270 280 290 300
KLCTVIMKSN GSFPVNRRRM DAARRAQELE MEMLSSTSPP ERTRYSPIPP
310 320 330 340 350
SHHQLTLPDP SHHGLHSNPD SPAKPEKNGH AKIVNPRIAK FFEIQTMPNG
360 370 380 390 400
KTRTSLKTMS RRKLSQQKEK KATQMLAIVL GVFIICWLPF FITHILNIHC
410 420 430 440
DCNIPPVLYS AFTWLGYVNS AVNPIIYTTF NIEFRKAFMK ILHC
The sequence of this isoform differs from the canonical sequence as follows:
242-270: Missing.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 99 | E → D in CAA37373 (PubMed:2139615).Curated | 1 | |
Sequence conflicti | 173 | G → R in CAA37373 (PubMed:2139615).Curated | 1 | |
Sequence conflicti | 180 | N → G in CAA37373 (PubMed:2139615).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001871 | 242 – 270 | Missing in isoform Short. 1 PublicationAdd BLAST | 29 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M36831 mRNA Translation: AAA41075.1 X53278 mRNA Translation: CAA37373.1 X56065 mRNA Translation: CAA39543.1 M32241 mRNA Translation: AAA41074.1 |
PIRi | S08146 |
RefSeqi | NP_036679.1, NM_012547.1 [P61169-1] XP_006243041.1, XM_006242979.3 [P61169-2] |
Genome annotation databases
Ensembli | ENSRNOT00000045944; ENSRNOP00000043759; ENSRNOG00000008428 [P61169-1] ENSRNOT00000083419; ENSRNOP00000074235; ENSRNOG00000008428 [P61169-2] |
GeneIDi | 24318 |
KEGGi | rno:24318 |
UCSCi | RGD:2520, rat |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M36831 mRNA Translation: AAA41075.1 X53278 mRNA Translation: CAA37373.1 X56065 mRNA Translation: CAA39543.1 M32241 mRNA Translation: AAA41074.1 |
PIRi | S08146 |
RefSeqi | NP_036679.1, NM_012547.1 [P61169-1] XP_006243041.1, XM_006242979.3 [P61169-2] |
3D structure databases
SMRi | P61169 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 246498, 11 interactors |
CORUMi | P61169 |
DIPi | DIP-30883N |
IntActi | P61169, 5 interactors |
MINTi | P61169 |
STRINGi | 10116.ENSRNOP00000043759 |
Chemistry databases
BindingDBi | P61169 |
ChEMBLi | CHEMBL339 |
DrugCentrali | P61169 |
GuidetoPHARMACOLOGYi | 215 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P61169, 3 sites |
iPTMneti | P61169 |
PhosphoSitePlusi | P61169 |
Genome annotation databases
Ensembli | ENSRNOT00000045944; ENSRNOP00000043759; ENSRNOG00000008428 [P61169-1] ENSRNOT00000083419; ENSRNOP00000074235; ENSRNOG00000008428 [P61169-2] |
GeneIDi | 24318 |
KEGGi | rno:24318 |
UCSCi | RGD:2520, rat |
Organism-specific databases
CTDi | 1813 |
RGDi | 2520, Drd2 |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT00940000155539 |
InParanoidi | P61169 |
OMAi | HGLHSTA |
OrthoDBi | 1021787at2759 |
PhylomeDBi | P61169 |
TreeFami | TF334382 |
Enzyme and pathway databases
Reactomei | R-RNO-390651, Dopamine receptors |
Miscellaneous databases
PROi | PR:P61169 |
Gene expression databases
Bgeei | ENSRNOG00000008428, Expressed in brain and 7 other tissues |
Genevisiblei | P61169, RN |
Family and domain databases
InterProi | View protein in InterPro IPR001922, Dopamine_D2_rcpt IPR000929, Dopamine_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00567, DOPAMINED2R PR00242, DOPAMINER PR00237, GPCRRHODOPSN |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DRD2_RAT | |
Accessioni | P61169Primary (citable) accession number: P61169 Secondary accession number(s): P13953 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2004 |
Last sequence update: | May 10, 2004 | |
Last modified: | February 23, 2022 | |
This is version 160 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - SIMILARITY comments
Index of protein domains and families