Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Actin-related protein 3

Gene

ACTR3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9000076). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9000076). Seems to contact the pointed end of the daughter actin filament (PubMed:9000076). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:17220302, PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947). Plays a role in ciliogenesis (PubMed:20393563).4 Publications

GO - Molecular functioni

  • actin binding Source: UniProtKB-KW
  • ATP binding Source: UniProtKB-KW
  • structural constituent of cytoskeleton Source: FlyBase

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processCilium biogenesis/degradation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-2029482 Regulation of actin dynamics for phagocytic cup formation
R-HSA-3928662 EPHB-mediated forward signaling
R-HSA-5663213 RHO GTPases Activate WASPs and WAVEs
R-HSA-8856828 Clathrin-mediated endocytosis
SignaLinkiP61158
SIGNORiP61158

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-related protein 3
Alternative name(s):
Actin-like protein 3
Gene namesi
Name:ACTR3
Synonyms:ARP3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000115091.11
HGNCiHGNC:170 ACTR3
MIMi604222 gene
neXtProtiNX_P61158

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10096
OpenTargetsiENSG00000115091
PharmGKBiPA24489

Chemistry databases

DrugBankiDB08235 N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide

Polymorphism and mutation databases

DMDMi47117647

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00000890792 – 418Actin-related protein 3Add BLAST417

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei240N6-acetyllysineCombined sources1
Modified residuei244N6-acetyllysineCombined sources1
Modified residuei251N6-acetyllysineCombined sources1
Modified residuei254N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP61158
PaxDbiP61158
PeptideAtlasiP61158
PRIDEiP61158
ProteomicsDBi57268

2D gel databases

OGPiP32391

PTM databases

iPTMnetiP61158
PhosphoSitePlusiP61158
SwissPalmiP61158

Miscellaneous databases

PMAP-CutDBiP61158

Expressioni

Gene expression databases

BgeeiENSG00000115091 Expressed in 234 organ(s), highest expression level in esophagus squamous epithelium
CleanExiHS_ACTR3
ExpressionAtlasiP61158 baseline and differential
GenevisibleiP61158 HS

Organism-specific databases

HPAiCAB005085
HPA047016
HPA051683

Interactioni

Subunit structurei

Component of the Arp2/3 complex composed of ACTR2/ARP2, ACTR3/ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC (PubMed:9000076, PubMed:9230079, PubMed:11741539). Interacts with WHDC1 (PubMed:18614018). Interacts weakly with MEFV (PubMed:19109554).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
JUNDP175352EBI-351428,EBI-2682803

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115403, 104 interactors
CORUMiP61158
DIPiDIP-33140N
IntActiP61158, 49 interactors
MINTiP61158
STRINGi9606.ENSP00000263238

Structurei

3D structure databases

ProteinModelPortaliP61158
SMRiP61158
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family. ARP3 subfamily.Curated

Phylogenomic databases

eggNOGiKOG0678 Eukaryota
COG5277 LUCA
GeneTreeiENSGT00550000074695
HOGENOMiHOG000233339
HOVERGENiHBG003771
InParanoidiP61158
KOiK18584
OMAiAIKHIPI
OrthoDBiEOG091G0BPD
PhylomeDBiP61158
TreeFamiTF300644

Family and domain databases

InterProiView protein in InterPro
IPR004000 Actin
IPR020902 Actin/actin-like_CS
IPR015623 Arp3
PANTHERiPTHR11937 PTHR11937, 1 hit
PTHR11937:SF175 PTHR11937:SF175, 1 hit
PfamiView protein in Pfam
PF00022 Actin, 2 hits
SMARTiView protein in SMART
SM00268 ACTIN, 1 hit
PROSITEiView protein in PROSITE
PS01132 ACTINS_ACT_LIKE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

P61158-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGRLPACVV DCGTGYTKLG YAGNTEPQFI IPSCIAIKES AKVGDQAQRR
60 70 80 90 100
VMKGVDDLDF FIGDEAIEKP TYATKWPIRH GIVEDWDLME RFMEQVIFKY
110 120 130 140 150
LRAEPEDHYF LLTEPPLNTP ENREYTAEIM FESFNVPGLY IAVQAVLALA
160 170 180 190 200
ASWTSRQVGE RTLTGTVIDS GDGVTHVIPV AEGYVIGSCI KHIPIAGRDI
210 220 230 240 250
TYFIQQLLRD REVGIPPEQS LETAKAVKER YSYVCPDLVK EFNKYDTDGS
260 270 280 290 300
KWIKQYTGIN AISKKEFSID VGYERFLGPE IFFHPEFANP DFTQPISEVV
310 320 330 340 350
DEVIQNCPID VRRPLYKNIV LSGGSTMFRD FGRRLQRDLK RTVDARLKLS
360 370 380 390 400
EELSGGRLKP KPIDVQVITH HMQRYAVWFG GSMLASTPEF YQVCHTKKDY
410
EEIGPSICRH NPVFGVMS
Length:418
Mass (Da):47,371
Last modified:January 23, 2007 - v3
Checksum:i23E5564198B81C63
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B4DXW1B4DXW1_HUMAN
Actin-related protein 3
ACTR3
367Annotation score:
F8WEW2F8WEW2_HUMAN
Actin-related protein 3
ACTR3
34Annotation score:
F8WE84F8WE84_HUMAN
Actin-related protein 3
ACTR3
41Annotation score:
F8WDR7F8WDR7_HUMAN
Actin-related protein 3
ACTR3
84Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006083 mRNA Translation: AAB64188.1
AF127773 mRNA Translation: AAD51904.1
AK312659 mRNA Translation: BAG35542.1
AC110769 Genomic DNA Translation: AAX93226.1
CH471103 Genomic DNA Translation: EAW95179.1
BC044590 mRNA Translation: AAH44590.1
CCDSiCCDS33277.1
RefSeqiNP_005712.1, NM_005721.4
UniGeneiHs.433512
Hs.595349

Genome annotation databases

EnsembliENST00000263238; ENSP00000263238; ENSG00000115091
GeneIDi10096
KEGGihsa:10096
UCSCiuc002tkx.3 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006083 mRNA Translation: AAB64188.1
AF127773 mRNA Translation: AAD51904.1
AK312659 mRNA Translation: BAG35542.1
AC110769 Genomic DNA Translation: AAX93226.1
CH471103 Genomic DNA Translation: EAW95179.1
BC044590 mRNA Translation: AAH44590.1
CCDSiCCDS33277.1
RefSeqiNP_005712.1, NM_005721.4
UniGeneiHs.433512
Hs.595349

3D structure databases

ProteinModelPortaliP61158
SMRiP61158
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115403, 104 interactors
CORUMiP61158
DIPiDIP-33140N
IntActiP61158, 49 interactors
MINTiP61158
STRINGi9606.ENSP00000263238

Chemistry databases

DrugBankiDB08235 N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide

PTM databases

iPTMnetiP61158
PhosphoSitePlusiP61158
SwissPalmiP61158

Polymorphism and mutation databases

DMDMi47117647

2D gel databases

OGPiP32391

Proteomic databases

EPDiP61158
PaxDbiP61158
PeptideAtlasiP61158
PRIDEiP61158
ProteomicsDBi57268

Protocols and materials databases

DNASUi10096
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263238; ENSP00000263238; ENSG00000115091
GeneIDi10096
KEGGihsa:10096
UCSCiuc002tkx.3 human

Organism-specific databases

CTDi10096
DisGeNETi10096
EuPathDBiHostDB:ENSG00000115091.11
GeneCardsiACTR3
HGNCiHGNC:170 ACTR3
HPAiCAB005085
HPA047016
HPA051683
MIMi604222 gene
neXtProtiNX_P61158
OpenTargetsiENSG00000115091
PharmGKBiPA24489
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0678 Eukaryota
COG5277 LUCA
GeneTreeiENSGT00550000074695
HOGENOMiHOG000233339
HOVERGENiHBG003771
InParanoidiP61158
KOiK18584
OMAiAIKHIPI
OrthoDBiEOG091G0BPD
PhylomeDBiP61158
TreeFamiTF300644

Enzyme and pathway databases

ReactomeiR-HSA-2029482 Regulation of actin dynamics for phagocytic cup formation
R-HSA-3928662 EPHB-mediated forward signaling
R-HSA-5663213 RHO GTPases Activate WASPs and WAVEs
R-HSA-8856828 Clathrin-mediated endocytosis
SignaLinkiP61158
SIGNORiP61158

Miscellaneous databases

ChiTaRSiACTR3 human
GeneWikiiACTR3
GenomeRNAii10096
PMAP-CutDBiP61158
PROiPR:P61158
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000115091 Expressed in 234 organ(s), highest expression level in esophagus squamous epithelium
CleanExiHS_ACTR3
ExpressionAtlasiP61158 baseline and differential
GenevisibleiP61158 HS

Family and domain databases

InterProiView protein in InterPro
IPR004000 Actin
IPR020902 Actin/actin-like_CS
IPR015623 Arp3
PANTHERiPTHR11937 PTHR11937, 1 hit
PTHR11937:SF175 PTHR11937:SF175, 1 hit
PfamiView protein in Pfam
PF00022 Actin, 2 hits
SMARTiView protein in SMART
SM00268 ACTIN, 1 hit
PROSITEiView protein in PROSITE
PS01132 ACTINS_ACT_LIKE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiARP3_HUMAN
AccessioniPrimary (citable) accession number: P61158
Secondary accession number(s): P32391, Q53QM2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 154 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again