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Protein

Ubiquitin-conjugating enzyme E2 K

Gene

Ube2k

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. Involved in stabilization of CASP12 during ER stress-mediated amyloid-beta neurotoxicity probably by inhibiting proteasome activity; in vitro ubiquitinates CASP12.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei92Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 K (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme K
Huntingtin-interacting protein 2
Short name:
HIP-2
Ubiquitin carrier protein
Ubiquitin-conjugating enzyme E2-25 kDa
Short name:
Ubiquitin-conjugating enzyme E2(25K)
Short name:
Ubiquitin-conjugating enzyme E2-25K
Ubiquitin-protein ligase
Gene namesi
Name:Ube2k
Synonyms:Hip2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1858216 Ube2k

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Neurons are resistant to amyloid-beta neurotoxicity. Significantly lower CASP12 expression in brain.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000824442 – 200Ubiquitin-conjugating enzyme E2 KAdd BLAST199

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei14N6-acetyllysine; alternateBy similarity1
Cross-linki14Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki14Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Modified residuei159PhosphoserineBy similarity1

Post-translational modificationi

Sumoylation at Lys-14 impairs catalytic activity.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP61087
PaxDbiP61087
PeptideAtlasiP61087
PRIDEiP61087

2D gel databases

REPRODUCTION-2DPAGEiP61087

PTM databases

iPTMnetiP61087
PhosphoSitePlusiP61087
SwissPalmiP61087

Expressioni

Tissue specificityi

Expressed in the brain, with highest levels found in the mitral cells of the olfactory bulb, the pyramidal cell layer of the hippocampus and the Purkinje cells of the cerebellar cortex.1 Publication

Developmental stagei

Expressed at all stages of brain development and increases significantly between postnatal days 7 and 14.1 Publication

Gene expression databases

BgeeiENSMUSG00000029203 Expressed in 321 organ(s), highest expression level in primary oocyte
ExpressionAtlasiP61087 baseline and differential
GenevisibleiP61087 MM

Interactioni

Subunit structurei

Interacts with RNF138/NARF. Interacts with BRCA1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207286, 19 interactors
IntActiP61087, 14 interactors
MINTiP61087
STRINGi10090.ENSMUSP00000122471

Structurei

3D structure databases

ProteinModelPortaliP61087
SMRiP61087
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini160 – 200UBAPROSITE-ProRule annotationAdd BLAST41

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0418 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00670000098059
HOVERGENiHBG063308
InParanoidiP61087
KOiK04649
OMAiNKEIADC
OrthoDBiEOG091G0KVW
PhylomeDBiP61087
TreeFamiTF101127

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR015940 UBA
IPR009060 UBA-like_sf
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00627 UBA, 1 hit
PF00179 UQ_con, 1 hit
SMARTiView protein in SMART
SM00165 UBA, 1 hit
SUPFAMiSSF46934 SSF46934, 1 hit
SSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50030 UBA, 1 hit
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

P61087-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MANIAVQRIK REFKEVLKSE ETSKNQIKVD LVDENFTELR GEIAGPPDTP
60 70 80 90 100
YEGGRYQLEI KIPETYPFNP PKVRFITKIW HPNISSVTGA ICLDILKDQW
110 120 130 140 150
AAAMTLRTVL LSLQALLAAA EPDDPQDAVV ANQYKQNPEM FKQTARLWAH
160 170 180 190 200
VYAGAPVSSP EYTKKIENLC AMGFDRNAVI VALSSKSWDV ETATELLLSN
Length:200
Mass (Da):22,407
Last modified:January 23, 2007 - v3
Checksum:iE40668099ED25828
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z4U3D3Z4U3_MOUSE
Ubiquitin-conjugating enzyme E2 K
Ube2k
157Annotation score:
A0A0J9YU07A0A0J9YU07_MOUSE
Ubiquitin-conjugating enzyme E2 K
Ube2k
149Annotation score:
Q3V3R8Q3V3R8_MOUSE
Ubiquitin-conjugating enzyme E2 K
Ube2k Hip2
97Annotation score:
A0A0J9YUR9A0A0J9YUR9_MOUSE
Ubiquitin-conjugating enzyme E2 K
Ube2k
73Annotation score:
F8WIC2F8WIC2_MOUSE
Ubiquitin-conjugating enzyme E2 K
Ube2k
57Annotation score:
A0A0J9YUI1A0A0J9YUI1_MOUSE
Ubiquitin-conjugating enzyme E2 K
Ube2k
139Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti163T → P in BAA24927 (PubMed:10585161).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011081 mRNA Translation: BAA24927.1
BC002013 mRNA Translation: AAH02013.1
BC085311 mRNA Translation: AAH85311.1
AK006316 mRNA Translation: BAB24523.1
CCDSiCCDS39098.1
RefSeqiNP_058066.2, NM_016786.4
UniGeneiMm.319512
Mm.441014

Genome annotation databases

EnsembliENSMUST00000142407; ENSMUSP00000122471; ENSMUSG00000029203
GeneIDi53323
KEGGimmu:53323
UCSCiuc008xnu.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011081 mRNA Translation: BAA24927.1
BC002013 mRNA Translation: AAH02013.1
BC085311 mRNA Translation: AAH85311.1
AK006316 mRNA Translation: BAB24523.1
CCDSiCCDS39098.1
RefSeqiNP_058066.2, NM_016786.4
UniGeneiMm.319512
Mm.441014

3D structure databases

ProteinModelPortaliP61087
SMRiP61087
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207286, 19 interactors
IntActiP61087, 14 interactors
MINTiP61087
STRINGi10090.ENSMUSP00000122471

PTM databases

iPTMnetiP61087
PhosphoSitePlusiP61087
SwissPalmiP61087

2D gel databases

REPRODUCTION-2DPAGEiP61087

Proteomic databases

EPDiP61087
PaxDbiP61087
PeptideAtlasiP61087
PRIDEiP61087

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000142407; ENSMUSP00000122471; ENSMUSG00000029203
GeneIDi53323
KEGGimmu:53323
UCSCiuc008xnu.1 mouse

Organism-specific databases

CTDi3093
MGIiMGI:1858216 Ube2k

Phylogenomic databases

eggNOGiKOG0418 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00670000098059
HOVERGENiHBG063308
InParanoidiP61087
KOiK04649
OMAiNKEIADC
OrthoDBiEOG091G0KVW
PhylomeDBiP61087
TreeFamiTF101127

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiUbe2k mouse
PROiPR:P61087
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029203 Expressed in 321 organ(s), highest expression level in primary oocyte
ExpressionAtlasiP61087 baseline and differential
GenevisibleiP61087 MM

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR015940 UBA
IPR009060 UBA-like_sf
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00627 UBA, 1 hit
PF00179 UQ_con, 1 hit
SMARTiView protein in SMART
SM00165 UBA, 1 hit
SUPFAMiSSF46934 SSF46934, 1 hit
SSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50030 UBA, 1 hit
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUBE2K_MOUSE
AccessioniPrimary (citable) accession number: P61087
Secondary accession number(s): O54806
, P27924, Q16721, Q9CVV9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 146 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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