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Protein

Ras-related protein Rab-10

Gene

Rab10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. Finally, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion.By similarity4 Publications

Activity regulationi

Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP) (By similarity). That Rab is activated by the DENND4C guanine exchange factor (GEF).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi16 – 23GTPBy similarity8
Nucleotide bindingi18 – 24GTPBy similarity7
Nucleotide bindingi35 – 41GTPBy similarity7
Nucleotide bindingi64 – 68GTPBy similarity5
Nucleotide bindingi122 – 125GTPBy similarity4
Nucleotide bindingi152 – 154GTPBy similarity3

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GDP-dissociation inhibitor binding Source: MGI
  • GTPase activity Source: GO_Central
  • GTP binding Source: UniProtKB
  • myosin V binding Source: MGI

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation
R-MMU-8873719 RAB geranylgeranylation
R-MMU-8876198 RAB GEFs exchange GTP for GDP on RABs

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-10
Gene namesi
Name:Rab10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:105066 Rab10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi68Q → L: Probable constitutively active mutant unable to hydrolyze GTP; stimulates translocation of SLC2A4 to the plasma membrane. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001211471 – 200Ras-related protein Rab-10Add BLAST200

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei102N6-acetyllysineBy similarity1
Lipidationi199S-geranylgeranyl cysteineBy similarity1
Lipidationi200S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Prenylation

Proteomic databases

EPDiP61027
PaxDbiP61027
PeptideAtlasiP61027
PRIDEiP61027

PTM databases

iPTMnetiP61027
PhosphoSitePlusiP61027
SwissPalmiP61027

Expressioni

Inductioni

Up-regulated by LPS.1 Publication

Gene expression databases

BgeeiENSMUSG00000020671 Expressed in 289 organ(s), highest expression level in skin epidermis
CleanExiMM_RAB10
ExpressionAtlasiP61027 baseline and differential
GenevisibleiP61027 MM

Interactioni

Subunit structurei

Interacts with MYO5A; mediates the transport to the plasma membrane of SLC2A4/GLUT4 storage vesicles (PubMed:22908308). Interacts with GDI1 and maybe with GDI2; negatively regulates RAB10 association with membranes and activation (PubMed:19570034). Interacts (GDP-bound form) with LLGL1; the interaction is direct and promotes RAB10 association with membranes and activation through competition with the Rab inhibitor GDI1 (By similarity). Interacts with EXOC4; probably associates with the exocyst (By similarity). Interacts (GTP-bound form) with MICALCL, MICAL1, MICAL3, EHBP1 and EHBP1L1; at least in case of MICAL1 two molecules of RAB10 can bind to one molecule of MICAL1 (By similarity). Interacts with TBC1D13 (PubMed:22762500). Interacts with SEC16A (PubMed:27354378).By similarity4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202531, 6 interactors
DIPiDIP-37720N
IntActiP61027, 24 interactors
MINTiP61027
STRINGi10090.ENSMUSP00000021001

Structurei

3D structure databases

ProteinModelPortaliP61027
SMRiP61027
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi38 – 46Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0078 Eukaryota
ENOG410XPUI LUCA
GeneTreeiENSGT00890000139330
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiP61027
KOiK07903
OMAiMRNIEQH
OrthoDBiEOG091G0LA6
PhylomeDBiP61027
TreeFamiTF314097

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequencei

Sequence statusi: Complete.

P61027-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKKTYDLLF KLLLIGDSGV GKTCVLFRFS DDAFNTTFIS TIGIDFKIKT
60 70 80 90 100
VELQGKKIKL QIWDTAGQER FHTITTSYYR GAMGIMLVYD ITNGKSFENI
110 120 130 140 150
SKWLRNIDEH ANEDVERMLL GNKCDMDDKR VVPKGKGEQI AREHGIRFFE
160 170 180 190 200
TSAKANINIE KAFLTLAEDI LRKTPVKEPN SENVDISSGG GVTGWKSKCC
Length:200
Mass (Da):22,541
Last modified:April 26, 2004 - v1
Checksum:i7F02B8E8E46EE1E8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106N → H in BAB25858 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035646 mRNA Translation: AAC29313.1
AK008725 mRNA Translation: BAB25858.1
AK028320 mRNA Translation: BAC25878.1
AK087964 mRNA Translation: BAC40062.1
BC056374 mRNA Translation: AAH56374.1
CCDSiCCDS25782.1
RefSeqiNP_057885.1, NM_016676.5
UniGeneiMm.378993
Mm.486858

Genome annotation databases

EnsembliENSMUST00000021001; ENSMUSP00000021001; ENSMUSG00000020671
GeneIDi19325
KEGGimmu:19325
UCSCiuc007mwl.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035646 mRNA Translation: AAC29313.1
AK008725 mRNA Translation: BAB25858.1
AK028320 mRNA Translation: BAC25878.1
AK087964 mRNA Translation: BAC40062.1
BC056374 mRNA Translation: AAH56374.1
CCDSiCCDS25782.1
RefSeqiNP_057885.1, NM_016676.5
UniGeneiMm.378993
Mm.486858

3D structure databases

ProteinModelPortaliP61027
SMRiP61027
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202531, 6 interactors
DIPiDIP-37720N
IntActiP61027, 24 interactors
MINTiP61027
STRINGi10090.ENSMUSP00000021001

PTM databases

iPTMnetiP61027
PhosphoSitePlusiP61027
SwissPalmiP61027

Proteomic databases

EPDiP61027
PaxDbiP61027
PeptideAtlasiP61027
PRIDEiP61027

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021001; ENSMUSP00000021001; ENSMUSG00000020671
GeneIDi19325
KEGGimmu:19325
UCSCiuc007mwl.2 mouse

Organism-specific databases

CTDi10890
MGIiMGI:105066 Rab10

Phylogenomic databases

eggNOGiKOG0078 Eukaryota
ENOG410XPUI LUCA
GeneTreeiENSGT00890000139330
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiP61027
KOiK07903
OMAiMRNIEQH
OrthoDBiEOG091G0LA6
PhylomeDBiP61027
TreeFamiTF314097

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation
R-MMU-8873719 RAB geranylgeranylation
R-MMU-8876198 RAB GEFs exchange GTP for GDP on RABs

Miscellaneous databases

ChiTaRSiRab10 mouse
PROiPR:P61027
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020671 Expressed in 289 organ(s), highest expression level in skin epidermis
CleanExiMM_RAB10
ExpressionAtlasiP61027 baseline and differential
GenevisibleiP61027 MM

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAB10_MOUSE
AccessioniPrimary (citable) accession number: P61027
Secondary accession number(s): O88386, Q9D7X6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: November 7, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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