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UniProtKB - P61020 (RAB5B_HUMAN)
Protein
Ras-related protein Rab-5B
Gene
RAB5B
Organism
Homo sapiens (Human)
Status
Functioni
Protein transport. Probably involved in vesicular traffic.
By similarityCatalytic activityi
Activity regulationi
Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 27 – 35 | GTPCombined sources1 Publication | 9 | |
Nucleotide bindingi | 46 – 52 | GTPCombined sources1 Publication | 7 | |
Nucleotide bindingi | 75 – 79 | GTPBy similarity | 5 | |
Nucleotide bindingi | 133 – 136 | GTPCombined sources1 Publication | 4 | |
Nucleotide bindingi | 163 – 165 | GTPCombined sources1 Publication | 3 |
GO - Molecular functioni
- GDP binding Source: UniProtKB
- G protein activity Source: UniProtKB-EC
- GTPase activity Source: UniProtKB
- GTP binding Source: UniProtKB-KW
- GTP-dependent protein binding Source: UniProtKB
GO - Biological processi
- antigen processing and presentation Source: UniProtKB
- endocytosis Source: GO_Central
- endosome organization Source: Ensembl
- intracellular protein transport Source: GO_Central
- plasma membrane to endosome transport Source: UniProtKB
- regulation of endocytosis Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Biological process | Protein transport, Transport |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P61020 |
Reactomei | R-HSA-6798695, Neutrophil degranulation R-HSA-8854214, TBC/RABGAPs R-HSA-8856828, Clathrin-mediated endocytosis R-HSA-8873719, RAB geranylgeranylation R-HSA-8876198, RAB GEFs exchange GTP for GDP on RABs |
SignaLinki | P61020 |
SIGNORi | P61020 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:RAB5B |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9784, RAB5B |
MIMi | 179514, gene |
neXtProti | NX_P61020 |
VEuPathDBi | HostDB:ENSG00000111540 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Lipid-anchor 1 Publication; Cytoplasmic side 1 Publication
Endosome
- Early endosome membrane 1 Publication; Lipid-anchor 1 Publication
Other locations
- Melanosome 1 Publication
Note: Enriched in stage I melanosomes.1 Publication
Endosome
- early endosome Source: GO_Central
- early endosome membrane Source: Reactome
- endosome Source: UniProtKB
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- anchored component of synaptic vesicle membrane Source: Ensembl
- endocytic vesicle Source: GO_Central
- endomembrane system Source: GO_Central
- intracellular membrane-bounded organelle Source: HPA
- melanosome Source: UniProtKB-SubCell
- membrane Source: ProtInc
- secretory granule membrane Source: Reactome
Keywords - Cellular componenti
Cell membrane, Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 34 | S → N: Constitutively inactivated. Strongly reduces interaction with RIN2. 1 Publication | 1 | |
Mutagenesisi | 79 | Q → L: Constitutively active. 1 Publication | 1 | |
Mutagenesisi | 84 | S → A: Loss of phosphorylation. No effect on GDI1, GDI2, CHML and CHM binding. 1 Publication | 1 | |
Mutagenesisi | 84 | S → E: Phosphomimetic mutant. Loss of GDI1, GDI2, CHML and CHM binding. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 5869 |
OpenTargetsi | ENSG00000111540 |
PharmGKBi | PA34144 |
Miscellaneous databases
Pharosi | P61020, Tbio |
Genetic variation databases
BioMutai | RAB5B |
DMDMi | 46577637 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000121107 | 2 – 215 | Ras-related protein Rab-5BAdd BLAST | 214 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylthreonineCombined sources | 1 | |
Modified residuei | 84 | Phosphoserine; by LRRK21 Publication | 1 | |
Lipidationi | 212 | S-geranylgeranyl cysteineBy similarity | 1 | |
Lipidationi | 213 | S-geranylgeranyl cysteineBy similarity | 1 |
Post-translational modificationi
Phosphorylation of Ser-84 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.1 Publication
(Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3.1 Publication
Keywords - PTMi
Acetylation, Glycoprotein, Lipoprotein, Phosphoprotein, PrenylationProteomic databases
EPDi | P61020 |
jPOSTi | P61020 |
MassIVEi | P61020 |
MaxQBi | P61020 |
PaxDbi | P61020 |
PeptideAtlasi | P61020 |
PRIDEi | P61020 |
ProteomicsDBi | 4453 57253 [P61020-1] |
PTM databases
GlyGeni | P61020, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P61020 |
MetOSitei | P61020 |
PhosphoSitePlusi | P61020 |
SwissPalmi | P61020 |
Expressioni
Gene expression databases
Bgeei | ENSG00000111540, Expressed in C1 segment of cervical spinal cord and 247 other tissues |
ExpressionAtlasi | P61020, baseline and differential |
Genevisiblei | P61020, HS |
Organism-specific databases
HPAi | ENSG00000111540, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
P61020
With | #Exp. | IntAct |
---|---|---|
EEA1 [Q15075] | 3 | EBI-399401,EBI-298113 |
HTT [P42858] | 3 | EBI-399401,EBI-466029 |
LRRK2 [Q5S007] | 9 | EBI-399401,EBI-5323863 |
GO - Molecular functioni
- GTP-dependent protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 111807, 123 interactors |
IntActi | P61020, 23 interactors |
MINTi | P61020 |
STRINGi | 9606.ENSP00000353444 |
Miscellaneous databases
RNActi | P61020, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P61020 |
SMRi | P61020 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P61020 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 186 – 215 | DisorderedSequence analysisAdd BLAST | 30 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 49 – 57 | Effector regionSequence analysis | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 200 – 215 | Polar residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0092, Eukaryota |
GeneTreei | ENSGT01040000241446 |
HOGENOMi | CLU_041217_10_2_1 |
InParanoidi | P61020 |
OMAi | AVHFDIW |
PhylomeDBi | P61020 |
TreeFami | TF300199 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SMARTi | View protein in SMART SM00174, RHO, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P61020-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTSRSTARPN GQPQASKICQ FKLVLLGESA VGKSSLVLRF VKGQFHEYQE
60 70 80 90 100
STIGAAFLTQ SVCLDDTTVK FEIWDTAGQE RYHSLAPMYY RGAQAAIVVY
110 120 130 140 150
DITNQETFAR AKTWVKELQR QASPSIVIAL AGNKADLANK RMVEYEEAQA
160 170 180 190 200
YADDNSLLFM ETSAKTAMNV NDLFLAIAKK LPKSEPQNLG GAAGRSRGVD
210
LHEQSQQNKS QCCSN
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8VWZ7 | F8VWZ7_HUMAN | Ras-related protein Rab-5B | RAB5B | 79 | Annotation score: | ||
F8VUA5 | F8VUA5_HUMAN | Ras-related protein Rab-5B | RAB5B | 115 | Annotation score: | ||
F8VVZ0 | F8VVZ0_HUMAN | Ras-related protein Rab-5B | RAB5B | 100 | Annotation score: | ||
F8VPW9 | F8VPW9_HUMAN | Ras-related protein Rab-5B | RAB5B | 73 | Annotation score: |
Sequence cautioni
The sequence AAH40143 differs from that shown. Reason: Erroneous initiation.Curated
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_045301 | 106 – 146 | Missing in isoform 2. 1 PublicationAdd BLAST | 41 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X54871 mRNA Translation: CAA38653.1 AF498937 mRNA Translation: AAM21085.1 AK292597 mRNA Translation: BAF85286.1 AK296517 mRNA Translation: BAG59149.1 BX537408 mRNA Translation: CAD97650.1 AC034102 Genomic DNA No translation available. CH471054 Genomic DNA Translation: EAW96862.1 CH471054 Genomic DNA Translation: EAW96864.1 BC032740 mRNA Translation: AAH32740.1 BC040143 mRNA Translation: AAH40143.1 Different initiation. BC050558 mRNA Translation: AAH50558.2 BC056422 mRNA Translation: AAH56422.2 BC065298 mRNA Translation: AAH65298.2 |
CCDSi | CCDS58244.1 [P61020-2] CCDS8900.1 [P61020-1] |
PIRi | A43925 |
RefSeqi | NP_001238965.1, NM_001252036.1 [P61020-1] NP_001238966.1, NM_001252037.1 [P61020-2] NP_002859.1, NM_002868.3 [P61020-1] XP_005269108.1, XM_005269051.2 [P61020-1] XP_005269109.1, XM_005269052.2 [P61020-1] |
Genome annotation databases
Ensembli | ENST00000360299.10; ENSP00000353444.5; ENSG00000111540.16 ENST00000448789.2; ENSP00000391319.2; ENSG00000111540.16 [P61020-2] ENST00000553116.5; ENSP00000450168.1; ENSG00000111540.16 |
GeneIDi | 5869 |
KEGGi | hsa:5869 |
MANE-Selecti | ENST00000360299.10; ENSP00000353444.5; NM_002868.4; NP_002859.1 |
UCSCi | uc001siv.4, human [P61020-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X54871 mRNA Translation: CAA38653.1 AF498937 mRNA Translation: AAM21085.1 AK292597 mRNA Translation: BAF85286.1 AK296517 mRNA Translation: BAG59149.1 BX537408 mRNA Translation: CAD97650.1 AC034102 Genomic DNA No translation available. CH471054 Genomic DNA Translation: EAW96862.1 CH471054 Genomic DNA Translation: EAW96864.1 BC032740 mRNA Translation: AAH32740.1 BC040143 mRNA Translation: AAH40143.1 Different initiation. BC050558 mRNA Translation: AAH50558.2 BC056422 mRNA Translation: AAH56422.2 BC065298 mRNA Translation: AAH65298.2 |
CCDSi | CCDS58244.1 [P61020-2] CCDS8900.1 [P61020-1] |
PIRi | A43925 |
RefSeqi | NP_001238965.1, NM_001252036.1 [P61020-1] NP_001238966.1, NM_001252037.1 [P61020-2] NP_002859.1, NM_002868.3 [P61020-1] XP_005269108.1, XM_005269051.2 [P61020-1] XP_005269109.1, XM_005269052.2 [P61020-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2HEI | X-ray | 1.55 | A/B | 15-191 | [»] | |
AlphaFoldDBi | P61020 | |||||
SMRi | P61020 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111807, 123 interactors |
IntActi | P61020, 23 interactors |
MINTi | P61020 |
STRINGi | 9606.ENSP00000353444 |
PTM databases
GlyGeni | P61020, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P61020 |
MetOSitei | P61020 |
PhosphoSitePlusi | P61020 |
SwissPalmi | P61020 |
Genetic variation databases
BioMutai | RAB5B |
DMDMi | 46577637 |
Proteomic databases
EPDi | P61020 |
jPOSTi | P61020 |
MassIVEi | P61020 |
MaxQBi | P61020 |
PaxDbi | P61020 |
PeptideAtlasi | P61020 |
PRIDEi | P61020 |
ProteomicsDBi | 4453 57253 [P61020-1] |
Protocols and materials databases
Antibodypediai | 4131, 178 antibodies from 33 providers |
DNASUi | 5869 |
Genome annotation databases
Ensembli | ENST00000360299.10; ENSP00000353444.5; ENSG00000111540.16 ENST00000448789.2; ENSP00000391319.2; ENSG00000111540.16 [P61020-2] ENST00000553116.5; ENSP00000450168.1; ENSG00000111540.16 |
GeneIDi | 5869 |
KEGGi | hsa:5869 |
MANE-Selecti | ENST00000360299.10; ENSP00000353444.5; NM_002868.4; NP_002859.1 |
UCSCi | uc001siv.4, human [P61020-1] |
Organism-specific databases
CTDi | 5869 |
DisGeNETi | 5869 |
GeneCardsi | RAB5B |
HGNCi | HGNC:9784, RAB5B |
HPAi | ENSG00000111540, Low tissue specificity |
MIMi | 179514, gene |
neXtProti | NX_P61020 |
OpenTargetsi | ENSG00000111540 |
PharmGKBi | PA34144 |
VEuPathDBi | HostDB:ENSG00000111540 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0092, Eukaryota |
GeneTreei | ENSGT01040000241446 |
HOGENOMi | CLU_041217_10_2_1 |
InParanoidi | P61020 |
OMAi | AVHFDIW |
PhylomeDBi | P61020 |
TreeFami | TF300199 |
Enzyme and pathway databases
PathwayCommonsi | P61020 |
Reactomei | R-HSA-6798695, Neutrophil degranulation R-HSA-8854214, TBC/RABGAPs R-HSA-8856828, Clathrin-mediated endocytosis R-HSA-8873719, RAB geranylgeranylation R-HSA-8876198, RAB GEFs exchange GTP for GDP on RABs |
SignaLinki | P61020 |
SIGNORi | P61020 |
Miscellaneous databases
BioGRID-ORCSi | 5869, 11 hits in 1081 CRISPR screens |
ChiTaRSi | RAB5B, human |
EvolutionaryTracei | P61020 |
GeneWikii | RAB5B |
GenomeRNAii | 5869 |
Pharosi | P61020, Tbio |
PROi | PR:P61020 |
RNActi | P61020, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000111540, Expressed in C1 segment of cervical spinal cord and 247 other tissues |
ExpressionAtlasi | P61020, baseline and differential |
Genevisiblei | P61020, HS |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SMARTi | View protein in SMART SM00174, RHO, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RAB5B_HUMAN | |
Accessioni | P61020Primary (citable) accession number: P61020 Secondary accession number(s): A8K982 Q8IXL2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2004 |
Last sequence update: | April 26, 2004 | |
Last modified: | May 25, 2022 | |
This is version 174 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families