UniProtKB - P61012 (PPLA_CANLF)
Protein
Cardiac phospholamban
Gene
PLN
Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Functioni
Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca2+. Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation (By similarity).By similarity
GO - Molecular functioni
- ATPase inhibitor activity Source: BHF-UCL
- protein homodimerization activity Source: UniProtKB
GO - Biological processi
- adenylate cyclase-activating adrenergic receptor signaling pathway involved in heart process Source: Ensembl
- cardiac muscle tissue development Source: Ensembl
- cellular calcium ion homeostasis Source: Ensembl
- negative regulation of ATPase activity Source: BHF-UCL
- negative regulation of ATPase-coupled calcium transmembrane transporter activity Source: UniProtKB
- negative regulation of calcium ion binding Source: Ensembl
- negative regulation of calcium ion import into sarcoplasmic reticulum Source: BHF-UCL
- negative regulation of calcium ion transmembrane transporter activity Source: BHF-UCL
- negative regulation of heart rate Source: Ensembl
- Notch signaling pathway Source: Ensembl
- regulation of calcium ion transport Source: UniProtKB
- regulation of cardiac muscle cell contraction Source: Ensembl
- regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion Source: Ensembl
- regulation of relaxation of muscle Source: Ensembl
- regulation of ryanodine-sensitive calcium-release channel activity Source: Ensembl
- regulation of the force of heart contraction by cardiac conduction Source: Ensembl
Enzyme and pathway databases
Reactomei | R-CFA-5578775, Ion homeostasis R-CFA-936837, Ion transport by P-type ATPases |
Names & Taxonomyi
Protein namesi | Recommended name: Cardiac phospholambanShort name: PLB |
Gene namesi | Name:PLN |
Organismi | Canis lupus familiaris (Dog) (Canis familiaris) |
Taxonomic identifieri | 9615 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › |
Proteomesi |
|
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass membrane protein Sequence analysis
Mitochondrion
- Mitochondrion membrane By similarity; Single-pass membrane protein Sequence analysis
Other locations
- Sarcoplasmic reticulum membrane By similarity; Single-pass membrane protein Sequence analysis
- Membrane By similarity; Single-pass membrane protein Sequence analysis
Note: Colocalizes with HAX1 at the endoplasmic reticulum. Colocalizes with DMPK a the sarcoplasmic reticulum.By similarity
Endoplasmic reticulum
- sarcoplasmic reticulum membrane Source: UniProtKB
Mitochondrion
- mitochondrial membrane Source: UniProtKB-SubCell
Other locations
- calcium ion-transporting ATPase complex Source: Ensembl
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 31 | CytoplasmicSequence analysisAdd BLAST | 31 | |
Transmembranei | 32 – 52 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, Mitochondrion, Sarcoplasmic reticulumPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000191243 | 1 – 52 | Cardiac phospholambanAdd BLAST | 52 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionine1 Publication | 1 | |
Modified residuei | 16 | Phosphoserine; by PKA and DMPK2 Publications | 1 | |
Modified residuei | 17 | Phosphothreonine; by CaMK22 Publications | 1 | |
Lipidationi | 36 | S-palmitoyl cysteineBy similarity | 1 |
Post-translational modificationi
Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes (By similarity). Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation.By similarity2 Publications
Palmitoylated by ZDHHC16, promoting formation of the homopentamer.By similarity
Keywords - PTMi
Acetylation, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
PaxDbi | P61012 |
PTM databases
iPTMneti | P61012 |
Expressioni
Tissue specificityi
Heart.1 Publication
Gene expression databases
Bgeei | ENSCAFG00000029347, Expressed in heart and 46 other tissues |
Interactioni
Subunit structurei
Homopentamer (PubMed:3753485).
Interacts with HAX1. Interact with ATP2A2; the inhibition decreases ATP2A2 Ca2+ affinity.
Interacts with VMP1; VMP1 competes with PLN and SLN to prevent them from forming an inhibitory complex with ATP2A2.
By similarity1 PublicationGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
STRINGi | 9612.ENSCAFP00000036921 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P61012 |
SMRi | P61012 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the phospholamban family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S97F, Eukaryota |
GeneTreei | ENSGT00390000002403 |
HOGENOMi | CLU_214576_0_0_1 |
InParanoidi | P61012 |
OMAi | MEVPQQA |
TreeFami | TF330750 |
Family and domain databases
CDDi | cd20250, Phospholamban, 1 hit |
DisProti | DP00801 |
InterProi | View protein in InterPro IPR005984, PLB |
PANTHERi | PTHR21194, PTHR21194, 1 hit |
Pfami | View protein in Pfam PF04272, Phospholamban, 1 hit |
PIRSFi | PIRSF001665, PLB, 1 hit |
TIGRFAMsi | TIGR01294, P_lamban, 1 hit |
i Sequence
Sequence statusi: Complete.
P61012-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDKVQYLTRS AIRRASTIEM PQQARQNLQN LFINFCLILI CLLLICIIVM
LL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16012 mRNA Translation: AAA30884.1 Y00399 mRNA Translation: CAA68461.1 M35393 mRNA Translation: AAC41618.1 AY514751 Genomic DNA Translation: AAT44582.1 AY576872 Genomic DNA Translation: AAT48602.1 |
PIRi | A29002 |
RefSeqi | NP_001003332.1, NM_001003332.1 XP_013972393.1, XM_014116918.1 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16012 mRNA Translation: AAA30884.1 Y00399 mRNA Translation: CAA68461.1 M35393 mRNA Translation: AAC41618.1 AY514751 Genomic DNA Translation: AAT44582.1 AY576872 Genomic DNA Translation: AAT48602.1 |
PIRi | A29002 |
RefSeqi | NP_001003332.1, NM_001003332.1 XP_013972393.1, XM_014116918.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4KYT | X-ray | 2.83 | B/C | 1-52 | [»] | |
4Y3U | X-ray | 3.51 | B | 1-50 | [»] | |
BMRBi | P61012 | |||||
SMRi | P61012 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 9612.ENSCAFP00000036921 |
PTM databases
iPTMneti | P61012 |
Proteomic databases
PaxDbi | P61012 |
Genome annotation databases
Organism-specific databases
CTDi | 5350 |
Phylogenomic databases
eggNOGi | ENOG502S97F, Eukaryota |
GeneTreei | ENSGT00390000002403 |
HOGENOMi | CLU_214576_0_0_1 |
InParanoidi | P61012 |
OMAi | MEVPQQA |
TreeFami | TF330750 |
Enzyme and pathway databases
Reactomei | R-CFA-5578775, Ion homeostasis R-CFA-936837, Ion transport by P-type ATPases |
Gene expression databases
Bgeei | ENSCAFG00000029347, Expressed in heart and 46 other tissues |
Family and domain databases
CDDi | cd20250, Phospholamban, 1 hit |
DisProti | DP00801 |
InterProi | View protein in InterPro IPR005984, PLB |
PANTHERi | PTHR21194, PTHR21194, 1 hit |
Pfami | View protein in Pfam PF04272, Phospholamban, 1 hit |
PIRSFi | PIRSF001665, PLB, 1 hit |
TIGRFAMsi | TIGR01294, P_lamban, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PPLA_CANLF | |
Accessioni | P61012Primary (citable) accession number: P61012 Secondary accession number(s): P07473, Q56UH4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | April 1, 1988 | |
Last modified: | April 7, 2021 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families