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Protein

Ras-related protein Rab-8A

Gene

RAB8A

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis (By similarity). Together with MICALL2, may also regulate adherens junction assembly (By similarity). May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis (By similarity). Involved in autophagy (By similarity).By similarity

Activity regulationi

Activated in response to insulin.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 22GTPBy similarity8
Nucleotide bindingi17 – 22GTPBy similarity6
Nucleotide bindingi34 – 40GTPBy similarity7
Nucleotide bindingi63 – 67GTPBy similarity5
Nucleotide bindingi121 – 124GTPBy similarity4
Nucleotide bindingi152 – 154GTPBy similarity3

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTPase activity Source: GO_Central
  • GTP binding Source: UniProtKB
  • Rab GTPase binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processAutophagy, Cilium biogenesis/degradation, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CFA-2565942 Regulation of PLK1 Activity at G2/M Transition
R-CFA-5620912 Anchoring of the basal body to the plasma membrane
R-CFA-5620916 VxPx cargo-targeting to cilium
R-CFA-8854214 TBC/RABGAPs
R-CFA-8873719 RAB geranylgeranylation
R-CFA-8876198 RAB GEFs exchange GTP for GDP on RABs

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-8A
Alternative name(s):
Oncogene c-mel
Gene namesi
Name:RAB8A
Synonyms:MEL, RAB8
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Chromosome 20

Organism-specific databases

VGNCiVGNC:45295 RAB8A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001211291 – 204Ras-related protein Rab-8AAdd BLAST204
PropeptideiPRO_0000370792205 – 207Removed in mature formSequence analysis3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei181PhosphoserineBy similarity1
Modified residuei185PhosphoserineBy similarity1
Modified residuei204Cysteine methyl esterSequence analysis1
Lipidationi204S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP61007
PRIDEiP61007

Expressioni

Gene expression databases

BgeeiENSCAFG00000015782 Expressed in 3 organ(s), highest expression level in liver

Interactioni

Subunit structurei

Interacts (GTP-bound form) with MICALL1; regulates RAB8A association with recycling endosomes. Interacts with MICALL2; competes with RAB13 and is involved in E-cadherin endocytic recycling. Interacts (GTP-bound form) with MICAL1, MICALCL, MICAL3 and EHBP1L1; two molecules of RAB8A can bind to one molecule of the effector protein; ternary complexes of RAB8A, RAB13 and either MICAL1 or EHBP1L1 are possible. Interacts (GTP-bound form) with EHBP1. Interacts with EHD1. Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3. Interacts with RABIF, RIMS2, RPH3A and RPH3A. Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO. Interacts with BIRC6/bruce. Interacts with OCRL. Interacts with AHI1. Interacts with DCDC5.By similarity

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-42002N
IntActiP61007, 3 interactors
MINTiP61007
STRINGi9615.ENSCAFP00000037722

Structurei

3D structure databases

ProteinModelPortaliP61007
SMRiP61007
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi37 – 45Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0078 Eukaryota
ENOG410XPUI LUCA
GeneTreeiENSGT00890000139330
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiP61007
KOiK07901
OMAiHSFQNIR

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P61007-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKTYDYLFK LLLIGDSGVG KTCVLFRFSE DAFNSTFIST IGIDFKIRTI
60 70 80 90 100
ELDGKRIKLQ IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIR
110 120 130 140 150
NWIRNIEEHA SADVEKMILG NKCDVNDKRQ VSKERGEKLA LDYGIKFMET
160 170 180 190 200
SAKANINVEN AFFTLARDIK AKMDKKLEGN SPQGSNQGVK ITPDQQKRSS

FFRCVLL
Length:207
Mass (Da):23,668
Last modified:April 26, 2004 - v1
Checksum:iAA52DBF54A2CD056
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J9NWR1J9NWR1_CANLF
Ras-related protein Rab-8A
RAB8A
228Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56385 mRNA Translation: CAB56776.1
PIRiB36364
RefSeqiNP_001003152.1, NM_001003152.1
UniGeneiCfa.3637

Genome annotation databases

EnsembliENSCAFT00000025007; ENSCAFP00000023207; ENSCAFG00000015782
GeneIDi403773
KEGGicfa:403773

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56385 mRNA Translation: CAB56776.1
PIRiB36364
RefSeqiNP_001003152.1, NM_001003152.1
UniGeneiCfa.3637

3D structure databases

ProteinModelPortaliP61007
SMRiP61007
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-42002N
IntActiP61007, 3 interactors
MINTiP61007
STRINGi9615.ENSCAFP00000037722

Proteomic databases

PaxDbiP61007
PRIDEiP61007

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000025007; ENSCAFP00000023207; ENSCAFG00000015782
GeneIDi403773
KEGGicfa:403773

Organism-specific databases

CTDi4218
VGNCiVGNC:45295 RAB8A

Phylogenomic databases

eggNOGiKOG0078 Eukaryota
ENOG410XPUI LUCA
GeneTreeiENSGT00890000139330
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiP61007
KOiK07901
OMAiHSFQNIR

Enzyme and pathway databases

ReactomeiR-CFA-2565942 Regulation of PLK1 Activity at G2/M Transition
R-CFA-5620912 Anchoring of the basal body to the plasma membrane
R-CFA-5620916 VxPx cargo-targeting to cilium
R-CFA-8854214 TBC/RABGAPs
R-CFA-8873719 RAB geranylgeranylation
R-CFA-8876198 RAB GEFs exchange GTP for GDP on RABs

Gene expression databases

BgeeiENSCAFG00000015782 Expressed in 3 organ(s), highest expression level in liver

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAB8A_CANLF
AccessioniPrimary (citable) accession number: P61007
Secondary accession number(s): P24407
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: November 7, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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