UniProtKB - P61006 (RAB8A_HUMAN)
Ras-related protein Rab-8A
RAB8A
Functioni
Activity regulationi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 17 – 22 | GTPCombined sources1 Publication | 6 | |
Nucleotide bindingi | 34 – 40 | GTPCombined sources1 Publication | 7 | |
Nucleotide bindingi | 63 – 67 | GTPCombined sourcesBy similarity1 Publication | 5 | |
Nucleotide bindingi | 121 – 124 | GTPCombined sources1 Publication | 4 | |
Nucleotide bindingi | 152 – 154 | GTPCombined sourcesBy similarity1 Publication | 3 |
GO - Molecular functioni
- GDP binding Source: UniProtKB
- GTPase activity Source: UniProtKB
- GTP binding Source: UniProtKB
- myosin V binding Source: UniProtKB
- protein kinase binding Source: Ensembl
- Rab GTPase binding Source: UniProtKB
GO - Biological processi
- autophagy Source: UniProtKB-KW
- axonogenesis Source: UniProtKB
- cellular response to insulin stimulus Source: UniProtKB
- cilium assembly Source: BHF-UCL
- Golgi vesicle fusion to target membrane Source: BHF-UCL
- neurotransmitter receptor transport to postsynaptic membrane Source: GO_Central
- post-translational protein modification Source: Reactome
- protein localization to plasma membrane Source: UniProtKB
- protein secretion Source: GO_Central
- regulation of autophagy Source: UniProtKB
- regulation of exocytosis Source: GO_Central
- regulation of long-term neuronal synaptic plasticity Source: Ensembl
- regulation of protein transport Source: Ensembl
- vesicle docking involved in exocytosis Source: BHF-UCL
Keywordsi
Biological process | Autophagy, Cilium biogenesis/degradation, Protein transport, Transport |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P61006 |
Reactomei | R-HSA-1445148, Translocation of SLC2A4 (GLUT4) to the plasma membrane R-HSA-2565942, Regulation of PLK1 Activity at G2/M Transition R-HSA-5620912, Anchoring of the basal body to the plasma membrane R-HSA-5620916, VxPx cargo-targeting to cilium R-HSA-8854214, TBC/RABGAPs R-HSA-8873719, RAB geranylgeranylation R-HSA-8876198, RAB GEFs exchange GTP for GDP on RABs |
SIGNORi | P61006 |
Names & Taxonomyi
Protein namesi | Recommended name: Ras-related protein Rab-8AAlternative name(s): Oncogene c-mel |
Gene namesi | Name:RAB8A Synonyms:MEL, RAB8 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000167461.11 |
HGNCi | HGNC:7007, RAB8A |
MIMi | 165040, gene |
neXtProti | NX_P61006 |
Subcellular locationi
Cytoskeleton
- centriole By similarity
- cilium basal body 1 Publication
- cilium axoneme 1 Publication
Plasma membrane
- Cell membrane 1 Publication1 Publication; Lipid-anchor 1 Publication; Cytoplasmic side 1 Publication
Endosome
- Recycling endosome membrane 1 Publication
Golgi apparatus
- Golgi apparatus 1 Publication
Other locations
- cilium 2 Publications
- phagosome 1 Publication
- phagosome membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
- Midbody 1 Publication
- Cytoplasm 1 Publication
Note: Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane (PubMed:15837803). In the GDP-bound form, present in the perinuclear region (PubMed:12221131). Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form (PubMed:12221131). Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis (PubMed:20890297). Localizes to tubular recycling endosome (PubMed:19864458). Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211). Non-phosphorylated RAB8A predominantly localizes to the cytoplasm whereas phosphorylated RAB8A localizes to the membrane (PubMed:26824392, PubMed:29125462, PubMed:30398148).8 Publications
Cytoskeleton
- centriole Source: UniProtKB-SubCell
- centrosome Source: MGI
- ciliary basal body Source: Ensembl
Cytosol
- cytosol Source: Reactome
Endosome
- endosome Source: GO_Central
- recycling endosome membrane Source: UniProtKB
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Golgi apparatus
- Golgi membrane Source: Reactome
- trans-Golgi network membrane Source: Reactome
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- ciliary base Source: Ensembl
- cilium Source: UniProtKB
- dendritic spine Source: GO_Central
- midbody Source: UniProtKB
- neuronal cell body Source: Ensembl
- non-motile cilium Source: BHF-UCL
- phagocytic vesicle Source: UniProtKB
- phagocytic vesicle membrane Source: UniProtKB-SubCell
- postsynaptic density Source: Ensembl
- synaptic vesicle Source: GO_Central
- trans-Golgi network transport vesicle Source: GO_Central
Keywords - Cellular componenti
Cell membrane, Cell projection, Cilium, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 22 | T → N: Loss of interaction with MICALL1. 1 Publication | 1 | |
Mutagenesisi | 67 | Q → L: Probable constitutively active mutant locked in the active GTP-bound form. Stimulates interaction with MICALL1. 1 Publication | 1 | |
Mutagenesisi | 72 | T → A: Loss of phosphorylation. No effect on the binding of GDP or GTP. Localizes primarily to the Golgi complex but does not affect membrane localization. 3 Publications | 1 | |
Mutagenesisi | 72 | T → E: Phosphomimetic mutant. No effect on the binding of GDP or GTP. Loss of GDI1, GDI2, CHM, CHML, RABGGTA, RABGGTB, RAB3IP, TBC1D15, and INPP5B binding. Increases localization to the cell membrane. 3 Publications | 1 |
Keywords - Diseasei
Proto-oncogeneOrganism-specific databases
DisGeNETi | 4218 |
OpenTargetsi | ENSG00000167461 |
PharmGKBi | PA30743 |
Miscellaneous databases
Pharosi | P61006, Tbio |
Polymorphism and mutation databases
BioMutai | RAB8A |
DMDMi | 46810392 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000121130 | 1 – 204 | Ras-related protein Rab-8AAdd BLAST | 204 | |
PropeptideiPRO_0000370793 | 205 – 207 | Removed in mature formSequence analysis | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 72 | Phosphothreonine; by LRRK23 Publications | 1 | |
Modified residuei | 181 | PhosphoserineCombined sources | 1 | |
Modified residuei | 185 | PhosphoserineCombined sources | 1 | |
Modified residuei | 204 | Cysteine methyl esterSequence analysis | 1 | |
Lipidationi | 204 | S-geranylgeranyl cysteine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Methylation, Phosphoprotein, PrenylationProteomic databases
EPDi | P61006 |
jPOSTi | P61006 |
MassIVEi | P61006 |
MaxQBi | P61006 |
PaxDbi | P61006 |
PeptideAtlasi | P61006 |
PRIDEi | P61006 |
ProteomicsDBi | 57245 [P61006-1] |
PTM databases
iPTMneti | P61006 |
PhosphoSitePlusi | P61006 |
SwissPalmi | P61006 |
Expressioni
Gene expression databases
Bgeei | ENSG00000167461, Expressed in liver and 250 other tissues |
ExpressionAtlasi | P61006, baseline and differential |
Genevisiblei | P61006, HS |
Organism-specific databases
HPAi | ENSG00000167461, Low tissue specificity |
Interactioni
Subunit structurei
Interacts (GTP-bound form) with MICALL1; regulates RAB8A association with recycling endosomes (By similarity).
Interacts with MICALL2; competes with RAB13 and is involved in E-cadherin endocytic recycling (By similarity).
Interacts (GTP-bound form) with MICAL1, MICALCL, MICAL3, EHBP1 and EHBP1L1; at least in case of MICAL1, MICALCL, MICAL3 and EHBP1L1 two molecules of RAB8A can bind to one molecule of the effector protein; ternary complexes of RAB8A, RAB13 and either MICAL1 or EHBP1L1 are possible (PubMed:27552051).
Interacts with EHD1 (PubMed:19864458).
Interacts with MAP4K2 and SYTL4 (By similarity).
Interacts with SGSM1 and SGSM3 (By similarity).
Interacts with RABIF, RIMS2, RPH3A and RPH3A (By similarity).
Interacts with OPTN (PubMed:15837803).
Interacts with RAB3IP (PubMed:12221131).
Interacts with MYO5B (PubMed:21282656).
Interacts with PIFO (PubMed:20643351).
Interacts with BIRC6/bruce (PubMed:18329369).
Interacts with OCRL (PubMed:22543976, PubMed:21378754).
Interacts with AHI1 (By similarity).
Interacts with DCDC1 (PubMed:22159412).
Interacts with LRRK2; interaction facilitates phosphorylation of Thr-72 (PubMed:26824392).
Interacts with RAB31P, GDI1, GDI2, CHM, CHML, RABGGTA, RABGGTB, TBC1D15 and INPP5B; these interactions are dependent on Thr-72 not being phosphorylated (PubMed:26824392, PubMed:29125462).
Interacts with RILPL1 and RILPL2; these interactions are dependent on the phosphorylation of Thr-72 by LRRK2 (PubMed:29125462, PubMed:30398148).
By similarity13 PublicationsBinary interactionsi
Hide detailsP61006
GO - Molecular functioni
- myosin V binding Source: UniProtKB
- protein kinase binding Source: Ensembl
- Rab GTPase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 110382, 111 interactors |
CORUMi | P61006 |
DIPi | DIP-43703N |
IntActi | P61006, 91 interactors |
MINTi | P61006 |
STRINGi | 9606.ENSP00000300935 |
Miscellaneous databases
RNActi | P61006, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P61006 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 37 – 45 | Effector regionBy similarity | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0078, Eukaryota |
GeneTreei | ENSGT00940000157246 |
HOGENOMi | CLU_041217_23_1_1 |
InParanoidi | P61006 |
OMAi | KAPSWLS |
OrthoDBi | 1426655at2759 |
PhylomeDBi | P61006 |
TreeFami | TF314097 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAKTYDYLFK LLLIGDSGVG KTCVLFRFSE DAFNSTFIST IGIDFKIRTI
60 70 80 90 100
ELDGKRIKLQ IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIR
110 120 130 140 150
NWIRNIEEHA SADVEKMILG NKCDVNDKRQ VSKERGEKLA LDYGIKFMET
160 170 180 190 200
SAKANINVEN AFFTLARDIK AKMDKKLEGN SPQGSNQGVK ITPDQQKRSS
FFRCVLL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 177 – 183 | LEGNSPQ → WKATAP in AAB19681 (PubMed:1886711).Curated | 7 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056399 | 161 – 205 | AFFTL…FFRCV → RYQSKNGQKIGRQQPPGEQP GSQNHTGPAEEEQLFPMCSS VRNTA in isoform 2. 1 PublicationAdd BLAST | 45 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56741 mRNA Translation: CAA40065.1 S53268 mRNA Translation: AAB19681.1 AF498943 mRNA Translation: AAM21091.1 BT007184 mRNA Translation: AAP35848.1 AK293676 mRNA Translation: BAG57118.1 CR536583 mRNA Translation: CAG38820.1 AC008894 Genomic DNA No translation available. CR542274 mRNA Translation: CAG47070.1 CH471106 Genomic DNA Translation: EAW84526.1 BC002977 mRNA Translation: AAH02977.1 |
CCDSi | CCDS12339.1 [P61006-1] |
PIRi | B49647 |
RefSeqi | NP_005361.2, NM_005370.4 [P61006-1] |
Genome annotation databases
Ensembli | ENST00000300935; ENSP00000300935; ENSG00000167461 [P61006-1] ENST00000586682; ENSP00000467501; ENSG00000167461 [P61006-2] |
GeneIDi | 4218 |
KEGGi | hsa:4218 |
UCSCi | uc002ndn.5, human [P61006-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56741 mRNA Translation: CAA40065.1 S53268 mRNA Translation: AAB19681.1 AF498943 mRNA Translation: AAM21091.1 BT007184 mRNA Translation: AAP35848.1 AK293676 mRNA Translation: BAG57118.1 CR536583 mRNA Translation: CAG38820.1 AC008894 Genomic DNA No translation available. CR542274 mRNA Translation: CAG47070.1 CH471106 Genomic DNA Translation: EAW84526.1 BC002977 mRNA Translation: AAH02977.1 |
CCDSi | CCDS12339.1 [P61006-1] |
PIRi | B49647 |
RefSeqi | NP_005361.2, NM_005370.4 [P61006-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3QBT | X-ray | 2.00 | A/C/E/G | 6-176 | [»] | |
3TNF | X-ray | 2.50 | A | 6-176 | [»] | |
4LHV | X-ray | 1.95 | A/B/C/D/E | 6-176 | [»] | |
4LHW | X-ray | 1.55 | A/B/C/D/E | 6-176 | [»] | |
4LHX | X-ray | 3.05 | A/B | 1-184 | [»] | |
4LHY | X-ray | 3.10 | A/B | 1-184 | [»] | |
4LHZ | X-ray | 3.20 | A/B | 1-184 | [»] | |
4LI0 | X-ray | 3.30 | A/B | 1-184 | [»] | |
5SZI | X-ray | 2.85 | A | 1-207 | [»] | |
6RIR | X-ray | 1.77 | A/B | 1-181 | [»] | |
6STF | X-ray | 2.40 | A/B/C/D/E | 5-176 | [»] | |
6STG | X-ray | 2.50 | A/B | 5-176 | [»] | |
6ZSI | X-ray | 1.91 | A/B | 1-176 | [»] | |
6ZSJ | X-ray | 2.00 | A/B | 1-176 | [»] | |
SMRi | P61006 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110382, 111 interactors |
CORUMi | P61006 |
DIPi | DIP-43703N |
IntActi | P61006, 91 interactors |
MINTi | P61006 |
STRINGi | 9606.ENSP00000300935 |
PTM databases
iPTMneti | P61006 |
PhosphoSitePlusi | P61006 |
SwissPalmi | P61006 |
Polymorphism and mutation databases
BioMutai | RAB8A |
DMDMi | 46810392 |
Proteomic databases
EPDi | P61006 |
jPOSTi | P61006 |
MassIVEi | P61006 |
MaxQBi | P61006 |
PaxDbi | P61006 |
PeptideAtlasi | P61006 |
PRIDEi | P61006 |
ProteomicsDBi | 57245 [P61006-1] |
Protocols and materials databases
Antibodypediai | 27297, 274 antibodies |
DNASUi | 4218 |
Genome annotation databases
Ensembli | ENST00000300935; ENSP00000300935; ENSG00000167461 [P61006-1] ENST00000586682; ENSP00000467501; ENSG00000167461 [P61006-2] |
GeneIDi | 4218 |
KEGGi | hsa:4218 |
UCSCi | uc002ndn.5, human [P61006-1] |
Organism-specific databases
CTDi | 4218 |
DisGeNETi | 4218 |
EuPathDBi | HostDB:ENSG00000167461.11 |
GeneCardsi | RAB8A |
HGNCi | HGNC:7007, RAB8A |
HPAi | ENSG00000167461, Low tissue specificity |
MIMi | 165040, gene |
neXtProti | NX_P61006 |
OpenTargetsi | ENSG00000167461 |
PharmGKBi | PA30743 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0078, Eukaryota |
GeneTreei | ENSGT00940000157246 |
HOGENOMi | CLU_041217_23_1_1 |
InParanoidi | P61006 |
OMAi | KAPSWLS |
OrthoDBi | 1426655at2759 |
PhylomeDBi | P61006 |
TreeFami | TF314097 |
Enzyme and pathway databases
PathwayCommonsi | P61006 |
Reactomei | R-HSA-1445148, Translocation of SLC2A4 (GLUT4) to the plasma membrane R-HSA-2565942, Regulation of PLK1 Activity at G2/M Transition R-HSA-5620912, Anchoring of the basal body to the plasma membrane R-HSA-5620916, VxPx cargo-targeting to cilium R-HSA-8854214, TBC/RABGAPs R-HSA-8873719, RAB geranylgeranylation R-HSA-8876198, RAB GEFs exchange GTP for GDP on RABs |
SIGNORi | P61006 |
Miscellaneous databases
BioGRID-ORCSi | 4218, 4 hits in 846 CRISPR screens |
ChiTaRSi | RAB8A, human |
GeneWikii | RAB8A |
GenomeRNAii | 4218 |
Pharosi | P61006, Tbio |
PROi | PR:P61006 |
RNActi | P61006, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000167461, Expressed in liver and 250 other tissues |
ExpressionAtlasi | P61006, baseline and differential |
Genevisiblei | P61006, HS |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAB8A_HUMAN | |
Accessioni | P61006Primary (citable) accession number: P61006 Secondary accession number(s): B4DEK7, P24407, Q6FHV5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2004 |
Last sequence update: | April 26, 2004 | |
Last modified: | December 2, 2020 | |
This is version 180 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM