UniProtKB - P60981 (DEST_HUMAN)
Protein
Destrin
Gene
DSTN
Organism
Homo sapiens (Human)
Status
Functioni
Actin-depolymerizing protein. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Acts in a pH-independent manner.1 Publication
GO - Molecular functioni
- actin filament binding Source: UniProtKB
GO - Biological processi
- actin filament depolymerization Source: UniProtKB
- actin filament fragmentation Source: GO_Central
- actin filament severing Source: GO_Central
- actin polymerization or depolymerization Source: ProtInc
- cell motility Source: GO_Central
- positive regulation of actin filament depolymerization Source: Ensembl
Keywordsi
Molecular function | Actin-binding |
Enzyme and pathway databases
PathwayCommonsi | P60981 |
SIGNORi | P60981 |
Names & Taxonomyi
Protein namesi | Recommended name: DestrinAlternative name(s): Actin-depolymerizing factor Short name: ADF |
Gene namesi | Name:DSTN Synonyms:ACTDP, DSN |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000125868.15 |
HGNCi | HGNC:15750, DSTN |
MIMi | 609114, gene |
neXtProti | NX_P60981 |
Subcellular locationi
Cytoskeleton
- actin cytoskeleton Source: GO_Central
- cortical actin cytoskeleton Source: GO_Central
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 11034 |
OpenTargetsi | ENSG00000125868 |
PharmGKBi | PA27509 |
Miscellaneous databases
Pharosi | P60981, Tbio |
Chemistry databases
DrugBanki | DB04147, Dodecyldimethylamine N-oxide |
Polymorphism and mutation databases
BioMutai | DSTN |
DMDMi | 46577586 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000214918 | 2 – 165 | DestrinAdd BLAST | 164 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources1 Publication | 1 | |
Modified residuei | 3 | PhosphoserineCombined sources | 1 | |
Modified residuei | 19 | N6-acetyllysineCombined sources | 1 |
Post-translational modificationi
ISGylated.1 Publication
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P60981 |
jPOSTi | P60981 |
MassIVEi | P60981 |
PaxDbi | P60981 |
PeptideAtlasi | P60981 |
PRIDEi | P60981 |
ProteomicsDBi | 57240 [P60981-1] 57241 [P60981-2] |
2D gel databases
OGPi | P18282 |
REPRODUCTION-2DPAGEi | IPI00473014 |
UCD-2DPAGEi | P60981 |
PTM databases
iPTMneti | P60981 |
MetOSitei | P60981 |
PhosphoSitePlusi | P60981 |
SwissPalmi | P60981 |
Expressioni
Tissue specificityi
Widely distributed in various tissues.
Gene expression databases
Bgeei | ENSG00000125868, Expressed in blood vessel layer and 255 other tissues |
ExpressionAtlasi | P60981, baseline and differential |
Genevisiblei | P60981, HS |
Organism-specific databases
HPAi | ENSG00000125868, Low tissue specificity |
Interactioni
Binary interactionsi
Hide detailsP60981
GO - Molecular functioni
- actin filament binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 116223, 125 interactors |
IntActi | P60981, 24 interactors |
MINTi | P60981 |
STRINGi | 9606.ENSP00000246069 |
Miscellaneous databases
RNActi | P60981, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 153 | ADF-HPROSITE-ProRule annotationAdd BLAST | 150 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 30 – 34 | Nuclear localization signalSequence analysis | 5 |
Sequence similaritiesi
Belongs to the actin-binding proteins ADF family.Curated
Phylogenomic databases
eggNOGi | KOG1735, Eukaryota |
GeneTreei | ENSGT00950000183000 |
InParanoidi | P60981 |
OMAi | WIPDTAK |
OrthoDBi | 1370477at2759 |
PhylomeDBi | P60981 |
TreeFami | TF328601 |
Family and domain databases
CDDi | cd11286, ADF_cofilin_like, 1 hit |
Gene3Di | 3.40.20.10, 1 hit |
InterProi | View protein in InterPro IPR002108, ADF-H IPR029006, ADF-H/Gelsolin-like_dom_sf IPR017904, ADF/Cofilin IPR029924, Dstn |
PANTHERi | PTHR11913, PTHR11913, 1 hit PTHR11913:SF18, PTHR11913:SF18, 1 hit |
Pfami | View protein in Pfam PF00241, Cofilin_ADF, 1 hit |
PRINTSi | PR00006, COFILIN |
SMARTi | View protein in SMART SM00102, ADF, 1 hit |
PROSITEi | View protein in PROSITE PS51263, ADF_H, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: P60981-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASGVQVADE VCRIFYDMKV RKCSTPEEIK KRKKAVIFCL SADKKCIIVE
60 70 80 90 100
EGKEILVGDV GVTITDPFKH FVGMLPEKDC RYALYDASFE TKESRKEELM
110 120 130 140 150
FFLWAPELAP LKSKMIYASS KDAIKKKFQG IKHECQANGP EDLNRACIAE
160
KLGGSLIVAF EGCPV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF6RFD5 | F6RFD5_HUMAN | Actin-depolymerizing factor | DSTN | 135 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_036459 | 139 | G → E in a colorectal cancer sample; somatic mutation. 1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_043069 | 1 – 17 | Missing in isoform 2. 1 PublicationAdd BLAST | 17 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S65738 mRNA Translation: AAB28361.1 CR457042 mRNA Translation: CAG33323.1 AK302338 mRNA Translation: BAG63668.1 AK312645 mRNA Translation: BAG35529.1 AL132765 Genomic DNA No translation available. CH471133 Genomic DNA Translation: EAX10274.1 CH471133 Genomic DNA Translation: EAX10275.1 CH471133 Genomic DNA Translation: EAX10276.1 BC009477 mRNA Translation: AAH09477.1 |
CCDSi | CCDS13127.1 [P60981-1] CCDS46580.1 [P60981-2] |
PIRi | A54184 |
RefSeqi | NP_001011546.1, NM_001011546.1 [P60981-2] NP_006861.1, NM_006870.3 [P60981-1] XP_011527444.1, XM_011529142.1 XP_011527445.1, XM_011529143.1 XP_011527446.1, XM_011529144.1 [P60981-2] |
Genome annotation databases
Ensembli | ENST00000246069; ENSP00000246069; ENSG00000125868 [P60981-1] ENST00000474024; ENSP00000476975; ENSG00000125868 [P60981-2] |
GeneIDi | 11034 |
KEGGi | hsa:11034 |
UCSCi | uc002wpq.4, human [P60981-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S65738 mRNA Translation: AAB28361.1 CR457042 mRNA Translation: CAG33323.1 AK302338 mRNA Translation: BAG63668.1 AK312645 mRNA Translation: BAG35529.1 AL132765 Genomic DNA No translation available. CH471133 Genomic DNA Translation: EAX10274.1 CH471133 Genomic DNA Translation: EAX10275.1 CH471133 Genomic DNA Translation: EAX10276.1 BC009477 mRNA Translation: AAH09477.1 |
CCDSi | CCDS13127.1 [P60981-1] CCDS46580.1 [P60981-2] |
PIRi | A54184 |
RefSeqi | NP_001011546.1, NM_001011546.1 [P60981-2] NP_006861.1, NM_006870.3 [P60981-1] XP_011527444.1, XM_011529142.1 XP_011527445.1, XM_011529143.1 XP_011527446.1, XM_011529144.1 [P60981-2] |
3D structure databases
SMRi | P60981 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 116223, 125 interactors |
IntActi | P60981, 24 interactors |
MINTi | P60981 |
STRINGi | 9606.ENSP00000246069 |
Chemistry databases
DrugBanki | DB04147, Dodecyldimethylamine N-oxide |
PTM databases
iPTMneti | P60981 |
MetOSitei | P60981 |
PhosphoSitePlusi | P60981 |
SwissPalmi | P60981 |
Polymorphism and mutation databases
BioMutai | DSTN |
DMDMi | 46577586 |
2D gel databases
OGPi | P18282 |
REPRODUCTION-2DPAGEi | IPI00473014 |
UCD-2DPAGEi | P60981 |
Proteomic databases
EPDi | P60981 |
jPOSTi | P60981 |
MassIVEi | P60981 |
PaxDbi | P60981 |
PeptideAtlasi | P60981 |
PRIDEi | P60981 |
ProteomicsDBi | 57240 [P60981-1] 57241 [P60981-2] |
Protocols and materials databases
Antibodypediai | 9232, 380 antibodies |
DNASUi | 11034 |
Genome annotation databases
Ensembli | ENST00000246069; ENSP00000246069; ENSG00000125868 [P60981-1] ENST00000474024; ENSP00000476975; ENSG00000125868 [P60981-2] |
GeneIDi | 11034 |
KEGGi | hsa:11034 |
UCSCi | uc002wpq.4, human [P60981-1] |
Organism-specific databases
CTDi | 11034 |
DisGeNETi | 11034 |
EuPathDBi | HostDB:ENSG00000125868.15 |
GeneCardsi | DSTN |
HGNCi | HGNC:15750, DSTN |
HPAi | ENSG00000125868, Low tissue specificity |
MIMi | 609114, gene |
neXtProti | NX_P60981 |
OpenTargetsi | ENSG00000125868 |
PharmGKBi | PA27509 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1735, Eukaryota |
GeneTreei | ENSGT00950000183000 |
InParanoidi | P60981 |
OMAi | WIPDTAK |
OrthoDBi | 1370477at2759 |
PhylomeDBi | P60981 |
TreeFami | TF328601 |
Enzyme and pathway databases
PathwayCommonsi | P60981 |
SIGNORi | P60981 |
Miscellaneous databases
BioGRID-ORCSi | 11034, 71 hits in 822 CRISPR screens |
ChiTaRSi | DSTN, human |
GenomeRNAii | 11034 |
Pharosi | P60981, Tbio |
PROi | PR:P60981 |
RNActi | P60981, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000125868, Expressed in blood vessel layer and 255 other tissues |
ExpressionAtlasi | P60981, baseline and differential |
Genevisiblei | P60981, HS |
Family and domain databases
CDDi | cd11286, ADF_cofilin_like, 1 hit |
Gene3Di | 3.40.20.10, 1 hit |
InterProi | View protein in InterPro IPR002108, ADF-H IPR029006, ADF-H/Gelsolin-like_dom_sf IPR017904, ADF/Cofilin IPR029924, Dstn |
PANTHERi | PTHR11913, PTHR11913, 1 hit PTHR11913:SF18, PTHR11913:SF18, 1 hit |
Pfami | View protein in Pfam PF00241, Cofilin_ADF, 1 hit |
PRINTSi | PR00006, COFILIN |
SMARTi | View protein in SMART SM00102, ADF, 1 hit |
PROSITEi | View protein in PROSITE PS51263, ADF_H, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DEST_HUMAN | |
Accessioni | P60981Primary (citable) accession number: P60981 Secondary accession number(s): B2R6N2 Q6IAW2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 163 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 20
Human chromosome 20: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations