UniProtKB - P60906 (SYH_ECOLI)
Protein
Histidine--tRNA ligase
Gene
hisS
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
- EC:6.1.1.21
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- histidine-tRNA ligase activity Source: EcoCyc
GO - Biological processi
- histidyl-tRNA aminoacylation Source: EcoCyc
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:HISS-MONOMER MetaCyc:HISS-MONOMER |
BRENDAi | 6.1.1.21, 2026 |
SABIO-RKi | P60906 |
Names & Taxonomyi
Protein namesi | Recommended name: Histidine--tRNA ligase (EC:6.1.1.21)Alternative name(s): Histidyl-tRNA synthetase Short name: HisRS |
Gene namesi | Name:hisS Ordered Locus Names:b2514, JW2498 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Chemistry databases
DrugBanki | DB03811, Histidinol DB04201, Histidyl-Adenosine Monophosphate |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000136161 | 2 – 424 | Histidine--tRNA ligaseAdd BLAST | 423 |
Proteomic databases
jPOSTi | P60906 |
PaxDbi | P60906 |
PRIDEi | P60906 |
2D gel databases
SWISS-2DPAGEi | P60906 |
Interactioni
Subunit structurei
Homodimer.
Protein-protein interaction databases
BioGRIDi | 4260589, 66 interactors |
DIPi | DIP-35894N |
IntActi | P60906, 2 interactors |
STRINGi | 511145.b2514 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P60906 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P60906 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-II aminoacyl-tRNA synthetase family.Curated
Phylogenomic databases
eggNOGi | COG0124, Bacteria |
HOGENOMi | CLU_025113_1_1_6 |
InParanoidi | P60906 |
PhylomeDBi | P60906 |
Family and domain databases
CDDi | cd00773, HisRS-like_core, 1 hit cd00859, HisRS_anticodon, 1 hit |
Gene3Di | 3.40.50.800, 1 hit |
HAMAPi | MF_00127, His_tRNA_synth, 1 hit |
InterProi | View protein in InterPro IPR006195, aa-tRNA-synth_II IPR004154, Anticodon-bd IPR036621, Anticodon-bd_dom_sf IPR015807, His-tRNA-ligase IPR041715, HisRS-like_core IPR004516, HisRS/HisZ IPR033656, HisRS_anticodon |
PANTHERi | PTHR43707, PTHR43707, 1 hit |
Pfami | View protein in Pfam PF03129, HGTP_anticodon, 1 hit PF13393, tRNA-synt_His, 1 hit |
PIRSFi | PIRSF001549, His-tRNA_synth, 1 hit |
TIGRFAMsi | TIGR00442, hisS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P60906-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKNIQAIRG MNDYLPGETA IWQRIEGTLK NVLGSYGYSE IRLPIVEQTP
60 70 80 90 100
LFKRAIGEVT DVVEKEMYTF EDRNGDSLTL RPEGTAGCVR AGIEHGLLYN
110 120 130 140 150
QEQRLWYIGP MFRHERPQKG RYRQFHQLGC EVFGLQGPDI DAELIMLTAR
160 170 180 190 200
WWRALGISEH VTLELNSIGS LEARANYRDA LVAFLEQHKE KLDEDCKRRM
210 220 230 240 250
YTNPLRVLDS KNPEVQALLN DAPALGDYLD EESREHFAGL CKLLESAGIA
260 270 280 290 300
YTVNQRLVRG LDYYNRTVFE WVTNSLGSQG TVCAGGRYDG LVEQLGGRAT
310 320 330 340 350
PAVGFAMGLE RLVLLVQAVN PEFKADPVVD IYLVASGADT QSAAMALAER
360 370 380 390 400
LRDELPGVKL MTNHGGGNFK KQFARADKWG ARVAVVLGES EVANGTAVVK
410 420
DLRSGEQTAV AQDSVAAHLR TLLG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11843 Genomic DNA Translation: AAA03226.1 U00096 Genomic DNA Translation: AAC75567.1 AP009048 Genomic DNA Translation: BAA16401.1 |
PIRi | A23890, SYECH |
RefSeqi | NP_417009.1, NC_000913.3 WP_001107167.1, NZ_STEB01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC75567; AAC75567; b2514 BAA16401; BAA16401; BAA16401 |
GeneIDi | 57732384 946989 |
KEGGi | ecj:JW2498 eco:b2514 |
PATRICi | fig|1411691.4.peg.4222 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11843 Genomic DNA Translation: AAA03226.1 U00096 Genomic DNA Translation: AAC75567.1 AP009048 Genomic DNA Translation: BAA16401.1 |
PIRi | A23890, SYECH |
RefSeqi | NP_417009.1, NC_000913.3 WP_001107167.1, NZ_STEB01000011.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1HTT | X-ray | 2.60 | A/B/C/D | 2-424 | [»] | |
1KMM | X-ray | 2.60 | A/B/C/D | 1-424 | [»] | |
1KMN | X-ray | 2.80 | A/B/C/D | 1-424 | [»] | |
2EL9 | X-ray | 2.70 | A/B/C/D | 1-424 | [»] | |
SMRi | P60906 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260589, 66 interactors |
DIPi | DIP-35894N |
IntActi | P60906, 2 interactors |
STRINGi | 511145.b2514 |
Chemistry databases
DrugBanki | DB03811, Histidinol DB04201, Histidyl-Adenosine Monophosphate |
2D gel databases
SWISS-2DPAGEi | P60906 |
Proteomic databases
jPOSTi | P60906 |
PaxDbi | P60906 |
PRIDEi | P60906 |
Genome annotation databases
EnsemblBacteriai | AAC75567; AAC75567; b2514 BAA16401; BAA16401; BAA16401 |
GeneIDi | 57732384 946989 |
KEGGi | ecj:JW2498 eco:b2514 |
PATRICi | fig|1411691.4.peg.4222 |
Organism-specific databases
EchoBASEi | EB0448 |
Phylogenomic databases
eggNOGi | COG0124, Bacteria |
HOGENOMi | CLU_025113_1_1_6 |
InParanoidi | P60906 |
PhylomeDBi | P60906 |
Enzyme and pathway databases
BioCyci | EcoCyc:HISS-MONOMER MetaCyc:HISS-MONOMER |
BRENDAi | 6.1.1.21, 2026 |
SABIO-RKi | P60906 |
Miscellaneous databases
EvolutionaryTracei | P60906 |
PROi | PR:P60906 |
Family and domain databases
CDDi | cd00773, HisRS-like_core, 1 hit cd00859, HisRS_anticodon, 1 hit |
Gene3Di | 3.40.50.800, 1 hit |
HAMAPi | MF_00127, His_tRNA_synth, 1 hit |
InterProi | View protein in InterPro IPR006195, aa-tRNA-synth_II IPR004154, Anticodon-bd IPR036621, Anticodon-bd_dom_sf IPR015807, His-tRNA-ligase IPR041715, HisRS-like_core IPR004516, HisRS/HisZ IPR033656, HisRS_anticodon |
PANTHERi | PTHR43707, PTHR43707, 1 hit |
Pfami | View protein in Pfam PF03129, HGTP_anticodon, 1 hit PF13393, tRNA-synt_His, 1 hit |
PIRSFi | PIRSF001549, His-tRNA_synth, 1 hit |
TIGRFAMsi | TIGR00442, hisS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYH_ECOLI | |
Accessioni | P60906Primary (citable) accession number: P60906 Secondary accession number(s): P04804 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 152 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families