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Protein

Ribose-phosphate pyrophosphokinase 1

Gene

Prps1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.

Catalytic activityi

ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Activity regulationi

Activated by magnesium and inorganic phosphate.

Pathwayi: 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I).
Proteins known to be involved in this subpathway in this organism are:
  1. Ribose-phosphate pyrophosphokinase 1 (Prps1), Ribose-phosphate pyrophosphokinase 2 (Prps2)
This subpathway is part of the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I), the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi128MagnesiumSequence analysis1
Metal bindingi130MagnesiumSequence analysis1
Binding sitei130ATPBy similarity1
Metal bindingi139MagnesiumSequence analysis1
Metal bindingi143MagnesiumSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi96 – 101ATPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processNucleotide biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-73843 5-Phosphoribose 1-diphosphate biosynthesis
SABIO-RKiP60892
UniPathwayi
UPA00087;UER00172

Names & Taxonomyi

Protein namesi
Recommended name:
Ribose-phosphate pyrophosphokinase 1 (EC:2.7.6.1)
Alternative name(s):
Phosphoribosyl pyrophosphate synthase I
Short name:
PRS-I
Gene namesi
Name:Prps1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi61955 Prps1

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001410731 – 318Ribose-phosphate pyrophosphokinase 1Add BLAST318

Proteomic databases

PaxDbiP60892
PRIDEiP60892

2D gel databases

World-2DPAGEi0004:P60892

PTM databases

iPTMnetiP60892
PhosphoSitePlusiP60892

Expressioni

Gene expression databases

BgeeiENSRNOG00000060262 Expressed in 10 organ(s), highest expression level in brain
GenevisibleiP60892 RN

Interactioni

Subunit structurei

Homodimer. The active form is probably a hexamer composed of 3 homodimers (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248196, 2 interactors
IntActiP60892, 2 interactors
STRINGi10116.ENSRNOP00000029405

Structurei

3D structure databases

ProteinModelPortaliP60892
SMRiP60892
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni212 – 227Binding of phosphoribosylpyrophosphateSequence analysisAdd BLAST16

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1448 Eukaryota
COG0462 LUCA
GeneTreeiENSGT00550000074583
HOGENOMiHOG000210451
HOVERGENiHBG001520
InParanoidiP60892
KOiK00948
OMAiFGWARQD
OrthoDBiEOG091G0ZG8
PhylomeDBiP60892
TreeFamiTF106366

Family and domain databases

CDDicd06223 PRTases_typeI, 1 hit
HAMAPiMF_00583_B RibP_PPkinase_B, 1 hit
InterProiView protein in InterPro
IPR000842 PRib_PP_synth_CS
IPR029099 Pribosyltran_N
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
IPR005946 Rib-P_diPkinase
IPR037515 Rib-P_diPkinase_bac
PANTHERiPTHR10210 PTHR10210, 1 hit
PfamiView protein in Pfam
PF14572 Pribosyl_synth, 1 hit
PF13793 Pribosyltran_N, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
TIGRFAMsiTIGR01251 ribP_PPkin, 1 hit
PROSITEiView protein in PROSITE
PS00114 PRPP_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

P60892-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPNIKIFSGS SHQDLSQKIA DRLGLELGKV VTKKFSNQET CVEIGESVRG
60 70 80 90 100
EDVYIVQSGC GEINDNLMEL LIMINACKIA SASRVTAVIP CFPYARQDKK
110 120 130 140 150
DKSRAPISAK LVANMLSVAG ADHIITMDLH ASQIQGFFDI PVDNLYAEPA
160 170 180 190 200
VLKWIRENIS EWRNCTIVSP DAGGAKRVTS IADRLNVDFA LIHKERKKAN
210 220 230 240 250
EVDRMVLVGD VKDRVAILVD DMADTCGTIC HAADKLLSAG ATRVYAILTH
260 270 280 290 300
GIFSGPAISR INNACFEAVV VTNTIPQEDK MKHCSKIQVI DISMILAEAI
310
RRTHNGESVS YLFSHVPL
Length:318
Mass (Da):34,834
Last modified:January 23, 2007 - v2
Checksum:i46D017E969908BA0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti163R → K in AAH88149 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17258 mRNA Translation: AAA41963.1
M29392 mRNA Translation: AAA41960.1
X16554 mRNA Translation: CAA34555.1
M31084
, M31078, M31079, M31080, M31082, M31083 Genomic DNA Translation: AAA41959.1
BC078853 mRNA Translation: AAH78853.1
BC088149 mRNA Translation: AAH88149.1
PIRiA35465 KIRTR1
RefSeqiNP_001009694.1, NM_001009694.1
NP_058939.1, NM_017243.2
XP_008762879.1, XM_008764657.2
UniGeneiRn.91470
Rn.9761

Genome annotation databases

EnsembliENSRNOT00000078582; ENSRNOP00000071101; ENSRNOG00000060262
GeneIDi29562
314140
KEGGirno:29562
rno:314140
UCSCiRGD:61955 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17258 mRNA Translation: AAA41963.1
M29392 mRNA Translation: AAA41960.1
X16554 mRNA Translation: CAA34555.1
M31084
, M31078, M31079, M31080, M31082, M31083 Genomic DNA Translation: AAA41959.1
BC078853 mRNA Translation: AAH78853.1
BC088149 mRNA Translation: AAH88149.1
PIRiA35465 KIRTR1
RefSeqiNP_001009694.1, NM_001009694.1
NP_058939.1, NM_017243.2
XP_008762879.1, XM_008764657.2
UniGeneiRn.91470
Rn.9761

3D structure databases

ProteinModelPortaliP60892
SMRiP60892
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248196, 2 interactors
IntActiP60892, 2 interactors
STRINGi10116.ENSRNOP00000029405

PTM databases

iPTMnetiP60892
PhosphoSitePlusiP60892

2D gel databases

World-2DPAGEi0004:P60892

Proteomic databases

PaxDbiP60892
PRIDEiP60892

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000078582; ENSRNOP00000071101; ENSRNOG00000060262
GeneIDi29562
314140
KEGGirno:29562
rno:314140
UCSCiRGD:61955 rat

Organism-specific databases

CTDi5631
RGDi61955 Prps1

Phylogenomic databases

eggNOGiKOG1448 Eukaryota
COG0462 LUCA
GeneTreeiENSGT00550000074583
HOGENOMiHOG000210451
HOVERGENiHBG001520
InParanoidiP60892
KOiK00948
OMAiFGWARQD
OrthoDBiEOG091G0ZG8
PhylomeDBiP60892
TreeFamiTF106366

Enzyme and pathway databases

UniPathwayi
UPA00087;UER00172

ReactomeiR-RNO-73843 5-Phosphoribose 1-diphosphate biosynthesis
SABIO-RKiP60892

Miscellaneous databases

PROiPR:P60892

Gene expression databases

BgeeiENSRNOG00000060262 Expressed in 10 organ(s), highest expression level in brain
GenevisibleiP60892 RN

Family and domain databases

CDDicd06223 PRTases_typeI, 1 hit
HAMAPiMF_00583_B RibP_PPkinase_B, 1 hit
InterProiView protein in InterPro
IPR000842 PRib_PP_synth_CS
IPR029099 Pribosyltran_N
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
IPR005946 Rib-P_diPkinase
IPR037515 Rib-P_diPkinase_bac
PANTHERiPTHR10210 PTHR10210, 1 hit
PfamiView protein in Pfam
PF14572 Pribosyl_synth, 1 hit
PF13793 Pribosyltran_N, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
TIGRFAMsiTIGR01251 ribP_PPkin, 1 hit
PROSITEiView protein in PROSITE
PS00114 PRPP_SYNTHASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPRPS1_RAT
AccessioniPrimary (citable) accession number: P60892
Secondary accession number(s): P09329, Q5M8A4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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