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UniProtKB - P60844 (AQPZ_ECOLI)
Protein
Aquaporin Z
Gene
aqpZ
Organism
Escherichia coli (strain K12)
Status
Functioni
Channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity.
3 PublicationsMiscellaneous
It is a remarkably rigid tetramer that does not dissociate even when solubilized in SDS.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 20 | Involved in tetramerization or stability of the tetramer | 1 | |
Sitei | 43 | Selectivity filter | 1 | |
Sitei | 174 | Selectivity filter | 1 | |
Sitei | 183 | Selectivity filter | 1 | |
Sitei | 189 | Selectivity filter | 1 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
- water channel activity Source: EcoCyc
GO - Biological processi
- cellular water homeostasis Source: EcoCyc
- response to osmotic stress Source: EcoCyc
- water transport Source: EcoCyc
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:AQPZ-MONOMER |
Protein family/group databases
TCDBi | 1.A.8.3.1, the major intrinsic protein (mip) family |
Names & Taxonomyi
Protein namesi | Recommended name: Aquaporin ZAlternative name(s): Bacterial nodulin-like intrinsic protein |
Gene namesi | Name:aqpZ Synonyms:bniP Ordered Locus Names:b0875, JW0859 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 2 Publications; Multi-pass membrane protein 2 Publications
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: EcoliWiki
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 8 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 9 – 29 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 30 – 33 | PeriplasmicSequence analysis | 4 | |
Transmembranei | 34 – 54 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 55 – 81 | CytoplasmicSequence analysisAdd BLAST | 27 | |
Transmembranei | 82 – 102 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 103 – 130 | PeriplasmicSequence analysisAdd BLAST | 28 | |
Transmembranei | 131 – 151 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 152 – 155 | CytoplasmicSequence analysis | 4 | |
Transmembranei | 156 – 176 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 177 – 201 | PeriplasmicSequence analysisAdd BLAST | 25 | |
Transmembranei | 202 – 222 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 223 – 231 | CytoplasmicSequence analysis | 9 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 9 | C → S: No effect. 1 Publication | 1 | |
Mutagenesisi | 20 | C → S: Loss of oligomerization; no alteration of water permeability. 1 Publication | 1 | |
Mutagenesisi | 183 | T → C: No effect. 1 Publication | 1 | |
Mutagenesisi | 189 | R → V or S: Loss of function. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB07349, (1S)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTANOYLOXY)METHYL]ETHYL OCTANOATE DB03152, B-2-Octylglucoside DB07923, octyl alpha-L-altropyranoside DB07924, octyl beta-D-galactopyranoside |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000063989 | 1 – 231 | Aquaporin ZAdd BLAST | 231 |
Proteomic databases
PaxDbi | P60844 |
Expressioni
Inductioni
By extracellular hypoosmotic conditions, especially during the mid-logarithmic phase of growth.
Interactioni
Subunit structurei
Homotetramer.
3 PublicationsBinary interactionsi
P60844
With | #Exp. | IntAct |
---|---|---|
itself | 4 | EBI-957663,EBI-957663 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4260000, 107 interactors |
DIPi | DIP-35499N |
STRINGi | 511145.b0875 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P60844 |
SMRi | P60844 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P60844 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 63 – 65 | NPA 1 | 3 | |
Motifi | 186 – 188 | NPA 2 | 3 |
Domaini
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0580, Bacteria |
HOGENOMi | CLU_020019_3_2_6 |
InParanoidi | P60844 |
OMAi | IFKALMY |
PhylomeDBi | P60844 |
Family and domain databases
CDDi | cd00333, MIP, 1 hit |
Gene3Di | 1.20.1080.10, 1 hit |
HAMAPi | MF_01146, Aquaporin_Z, 1 hit |
InterProi | View protein in InterPro IPR023271, Aquaporin-like IPR034294, Aquaporin_transptr IPR023743, Aquaporin_Z IPR000425, MIP IPR022357, MIP_CS |
PANTHERi | PTHR19139, PTHR19139, 1 hit |
Pfami | View protein in Pfam PF00230, MIP, 1 hit |
PRINTSi | PR00783, MINTRINSICP |
SUPFAMi | SSF81338, SSF81338, 1 hit |
TIGRFAMsi | TIGR00861, MIP, 1 hit |
PROSITEi | View protein in PROSITE PS00221, MIP, 1 hit |
i Sequence
Sequence statusi: Complete.
P60844-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFRKLAAECF GTFWLVFGGC GSAVLAAGFP ELGIGFAGVA LAFGLTVLTM
60 70 80 90 100
AFAVGHISGG HFNPAVTIGL WAGGRFPAKE VVGYVIAQVV GGIVAAALLY
110 120 130 140 150
LIASGKTGFD AAASGFASNG YGEHSPGGYS MLSALVVELV LSAGFLLVIH
160 170 180 190 200
GATDKFAPAG FAPIAIGLAL TLIHLISIPV TNTSVNPARS TAVAIFQGGW
210 220 230
ALEQLWFFWV VPIVGGIIGG LIYRTLLEKR D
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 14 | W → C in BAA08441 (Ref. 2) Curated | 1 | |
Sequence conflicti | 26 | A → P in BAA08441 (Ref. 2) Curated | 1 | |
Sequence conflicti | 99 | L → V in AAC43518 (PubMed:7493926).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U38664 Genomic DNA Translation: AAC43518.1 D49469 Genomic DNA Translation: BAA08441.1 U00096 Genomic DNA Translation: AAC73962.1 AP009048 Genomic DNA Translation: BAA35589.1 |
PIRi | C64826 |
RefSeqi | NP_415396.1, NC_000913.3 WP_001298299.1, NZ_SSZK01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC73962; AAC73962; b0875 BAA35589; BAA35589; BAA35589 |
GeneIDi | 58388674 945497 |
KEGGi | ecj:JW0859 eco:b0875 |
PATRICi | fig|1411691.4.peg.1402 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U38664 Genomic DNA Translation: AAC43518.1 D49469 Genomic DNA Translation: BAA08441.1 U00096 Genomic DNA Translation: AAC73962.1 AP009048 Genomic DNA Translation: BAA35589.1 |
PIRi | C64826 |
RefSeqi | NP_415396.1, NC_000913.3 WP_001298299.1, NZ_SSZK01000002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1RC2 | X-ray | 2.50 | A/B | 1-231 | [»] | |
2ABM | X-ray | 3.20 | A/B/C/D/E/F/G/H | 1-231 | [»] | |
2O9D | X-ray | 2.30 | A/B | 1-231 | [»] | |
2O9E | X-ray | 2.20 | A | 1-231 | [»] | |
2O9F | X-ray | 2.55 | A/B | 1-231 | [»] | |
2O9G | X-ray | 1.90 | A | 1-231 | [»] | |
3NK5 | X-ray | 2.40 | A/B | 1-231 | [»] | |
3NKA | X-ray | 2.50 | A/B | 1-231 | [»] | |
3NKC | X-ray | 3.10 | A/B | 1-231 | [»] | |
AlphaFoldDBi | P60844 | |||||
SMRi | P60844 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260000, 107 interactors |
DIPi | DIP-35499N |
STRINGi | 511145.b0875 |
Chemistry databases
DrugBanki | DB07349, (1S)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTANOYLOXY)METHYL]ETHYL OCTANOATE DB03152, B-2-Octylglucoside DB07923, octyl alpha-L-altropyranoside DB07924, octyl beta-D-galactopyranoside |
Protein family/group databases
TCDBi | 1.A.8.3.1, the major intrinsic protein (mip) family |
Proteomic databases
PaxDbi | P60844 |
Genome annotation databases
EnsemblBacteriai | AAC73962; AAC73962; b0875 BAA35589; BAA35589; BAA35589 |
GeneIDi | 58388674 945497 |
KEGGi | ecj:JW0859 eco:b0875 |
PATRICi | fig|1411691.4.peg.1402 |
Organism-specific databases
EchoBASEi | EB1283 |
Phylogenomic databases
eggNOGi | COG0580, Bacteria |
HOGENOMi | CLU_020019_3_2_6 |
InParanoidi | P60844 |
OMAi | IFKALMY |
PhylomeDBi | P60844 |
Enzyme and pathway databases
BioCyci | EcoCyc:AQPZ-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P60844 |
PROi | PR:P60844 |
Family and domain databases
CDDi | cd00333, MIP, 1 hit |
Gene3Di | 1.20.1080.10, 1 hit |
HAMAPi | MF_01146, Aquaporin_Z, 1 hit |
InterProi | View protein in InterPro IPR023271, Aquaporin-like IPR034294, Aquaporin_transptr IPR023743, Aquaporin_Z IPR000425, MIP IPR022357, MIP_CS |
PANTHERi | PTHR19139, PTHR19139, 1 hit |
Pfami | View protein in Pfam PF00230, MIP, 1 hit |
PRINTSi | PR00783, MINTRINSICP |
SUPFAMi | SSF81338, SSF81338, 1 hit |
TIGRFAMsi | TIGR00861, MIP, 1 hit |
PROSITEi | View protein in PROSITE PS00221, MIP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AQPZ_ECOLI | |
Accessioni | P60844Primary (citable) accession number: P60844 Secondary accession number(s): P48838, P75827, Q47159 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2004 |
Last sequence update: | April 13, 2004 | |
Last modified: | May 25, 2022 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families