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Protein

Cold-inducible RNA-binding protein

Gene

Cirbp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Promotes assembly of stress granules (SGs), when overexpressed. Seems to play an essential role in cold-induced suppression of cell proliferation. Acts as a translational repressor. Acts as a translational activator. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Repressor, RNA-binding
Biological processStress response

Names & Taxonomyi

Protein namesi
Recommended name:
Cold-inducible RNA-binding protein
Alternative name(s):
A18 hnRNP
Glycine-rich RNA-binding protein CIRP
Gene namesi
Name:Cirbp
Synonyms:Cirp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:893588 Cirbp

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi91R → K: Inhibits translational repression. 1 Publication1
Mutagenesisi94R → K: Inhibits stress granules localization and translational repression. 1 Publication1
Mutagenesisi101R → K: Inhibits translational repression. 1 Publication1
Mutagenesisi105R → K: Inhibits stress granules localization and translational repression. 1 Publication1
Mutagenesisi108R → K: Inhibits translational repression. 1 Publication1
Mutagenesisi110R → K: Inhibits translational repression. 1 Publication1
Mutagenesisi112R → K: Inhibits translational repression. 1 Publication1
Mutagenesisi116R → K: Inhibits stress granules localization and translational repression. 1 Publication1
Mutagenesisi121R → K: Inhibits translational repression. 1 Publication1
Mutagenesisi127R → K: Inhibits translational repression. 1 Publication1
Mutagenesisi131R → K: Inhibits translational repression. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000815041 – 172Cold-inducible RNA-binding proteinAdd BLAST172

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei130PhosphoserineBy similarity1
Modified residuei138PhosphoserineBy similarity1
Modified residuei146PhosphoserineBy similarity1
Modified residuei156PhosphoserineBy similarity1
Modified residuei159PhosphoserineBy similarity1
Modified residuei163PhosphoserineBy similarity1

Post-translational modificationi

Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs.1 Publication
Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation (By similarity).By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiP60824
MaxQBiP60824
PaxDbiP60824
PeptideAtlasiP60824
PRIDEiP60824

PTM databases

iPTMnetiP60824
PhosphoSitePlusiP60824

Expressioni

Tissue specificityi

Ubiquitous.

Inductioni

Up-regulated upon mild cold-shock and hypoxia.1 Publication

Gene expression databases

BgeeiENSMUSG00000045193 Expressed in 272 organ(s), highest expression level in retina
CleanExiMM_CIRBP
ExpressionAtlasiP60824 baseline and differential
GenevisibleiP60824 MM

Interactioni

Subunit structurei

Interacts with EIF4G1. Associates with ribosomes (By similarity).By similarity

Protein-protein interaction databases

BioGridi198716, 1 interactor
IntActiP60824, 2 interactors
MINTiP60824
STRINGi10090.ENSMUSP00000101004

Structurei

3D structure databases

ProteinModelPortaliP60824
SMRiP60824
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 84RRMPROSITE-ProRule annotationAdd BLAST79

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi92 – 158Gly-richAdd BLAST67

Domaini

Both the RRM domain and the arginine, glycine (RGG) rich domain are necessary for binding to the TXN 3'-untranslated region (By similarity). Both the RRM domain and the arginine, glycine (RGG) rich domain (RGG repeats) are necessary for optimal recruitment into SGs upon cellular stress. The C-terminal domain containing RGG repeats is necessary for translational repression.By similarity

Phylogenomic databases

eggNOGiKOG0118 Eukaryota
COG0724 LUCA
GeneTreeiENSGT00710000106295
HOGENOMiHOG000276232
HOVERGENiHBG107480
InParanoidiP60824
KOiK13195
OrthoDBiEOG091G13K0
PhylomeDBiP60824
TreeFamiTF354331

Family and domain databases

CDDicd12449 RRM_CIRBP_RBM3, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR034278 RBM3/CIRBP_RRM
IPR000504 RRM_dom
IPR003954 RRM_dom_euk
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SM00361 RRM_1, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P60824-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASDEGKLFV GGLSFDTNEQ ALEQVFSKYG QISEVVVVKD RETQRSRGFG
60 70 80 90 100
FVTFENIDDA KDAMMAMNGK SVDGRQIRVD QAGKSSDNRS RGYRGGSAGG
110 120 130 140 150
RGFFRGGRSR GRGFSRGGGD RGYGGGRFES RSGGYGGSRD YYASRSQGGS
160 170
YGYRSSGGSY RDSYDSYATH NE
Length:172
Mass (Da):18,607
Last modified:April 13, 2004 - v1
Checksum:iDA0E53088FBED4C8
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YU80D3YU80_MOUSE
Cold-inducible RNA-binding protein
Cirbp
108Annotation score:
K4DI65K4DI65_MOUSE
Cold inducible RNA binding protein,...
Cirbp mCG_13416
168Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78135 mRNA Translation: BAA11213.1
AK014655 mRNA Translation: BAB29491.1
AK132584 mRNA Translation: BAE21245.1
BC075699 mRNA Translation: AAH75699.1
CCDSiCCDS35975.1
RefSeqiNP_031731.1, NM_007705.2
UniGeneiMm.17898
Mm.487902

Genome annotation databases

EnsembliENSMUST00000105365; ENSMUSP00000101004; ENSMUSG00000045193
GeneIDi12696
KEGGimmu:12696
UCSCiuc007gce.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78135 mRNA Translation: BAA11213.1
AK014655 mRNA Translation: BAB29491.1
AK132584 mRNA Translation: BAE21245.1
BC075699 mRNA Translation: AAH75699.1
CCDSiCCDS35975.1
RefSeqiNP_031731.1, NM_007705.2
UniGeneiMm.17898
Mm.487902

3D structure databases

ProteinModelPortaliP60824
SMRiP60824
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198716, 1 interactor
IntActiP60824, 2 interactors
MINTiP60824
STRINGi10090.ENSMUSP00000101004

PTM databases

iPTMnetiP60824
PhosphoSitePlusiP60824

Proteomic databases

EPDiP60824
MaxQBiP60824
PaxDbiP60824
PeptideAtlasiP60824
PRIDEiP60824

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000105365; ENSMUSP00000101004; ENSMUSG00000045193
GeneIDi12696
KEGGimmu:12696
UCSCiuc007gce.1 mouse

Organism-specific databases

CTDi1153
MGIiMGI:893588 Cirbp

Phylogenomic databases

eggNOGiKOG0118 Eukaryota
COG0724 LUCA
GeneTreeiENSGT00710000106295
HOGENOMiHOG000276232
HOVERGENiHBG107480
InParanoidiP60824
KOiK13195
OrthoDBiEOG091G13K0
PhylomeDBiP60824
TreeFamiTF354331

Miscellaneous databases

ChiTaRSiCirbp mouse
PROiPR:P60824
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045193 Expressed in 272 organ(s), highest expression level in retina
CleanExiMM_CIRBP
ExpressionAtlasiP60824 baseline and differential
GenevisibleiP60824 MM

Family and domain databases

CDDicd12449 RRM_CIRBP_RBM3, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR034278 RBM3/CIRBP_RRM
IPR000504 RRM_dom
IPR003954 RRM_dom_euk
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SM00361 RRM_1, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCIRBP_MOUSE
AccessioniPrimary (citable) accession number: P60824
Secondary accession number(s): O09069
, O09148, Q3V1A6, Q61413
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: November 7, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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