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UniProtKB - P60785 (LEPA_ECOLI)
Protein
Elongation factor 4
Gene
lepA
Organism
Escherichia coli (strain K12)
Status
Functioni
Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.
UniRule annotation2 PublicationsCatalytic activityi
- EC:3.6.5.n1UniRule annotation1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 14 – 19 | GTPUniRule annotation | 6 | |
Nucleotide bindingi | 131 – 134 | GTPUniRule annotation | 4 |
GO - Molecular functioni
- GTPase activity Source: EcoCyc
- GTP binding Source: UniProtKB-UniRule
- guanosine tetraphosphate binding Source: EcoCyc
- identical protein binding Source: IntAct
- ribosomal large subunit binding Source: EcoCyc
- ribosomal small subunit binding Source: EcoCyc
- ribosome binding Source: GO_Central
- translation elongation factor activity Source: UniProtKB-UniRule
GO - Biological processi
- positive regulation of translation Source: GO_Central
- response to cold Source: EcoCyc
- response to pH Source: EcoCyc
- response to salt stress Source: EcoCyc
- ribosomal small subunit biogenesis Source: EcoCyc
Keywordsi
Molecular function | Hydrolase |
Biological process | Protein biosynthesis |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10529-MONOMER |
BRENDAi | 3.6.5.3, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)Short name: EF-4UniRule annotation Alternative name(s): Ribosomal back-translocase LepAUniRule annotation |
Gene namesi | Name:lepAUniRule annotation Ordered Locus Names:b2569, JW2553 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Cytosol
- cytosol Source: EcoCyc
Plasma Membrane
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000176271 | 1 – 599 | Elongation factor 4Add BLAST | 599 |
Proteomic databases
jPOSTi | P60785 |
PaxDbi | P60785 |
PRIDEi | P60785 |
Interactioni
Binary interactionsi
P60785
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-544544,EBI-544544 |
zntB [P64423] | 4 | EBI-544544,EBI-553267 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4260596, 259 interactors 851389, 3 interactors |
DIPi | DIP-29868N |
IntActi | P60785, 62 interactors |
STRINGi | 511145.b2569 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P60785 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P60785 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 184 | tr-type GAdd BLAST | 183 |
Sequence similaritiesi
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | COG0481, Bacteria |
HOGENOMi | CLU_009995_3_3_6 |
InParanoidi | P60785 |
PhylomeDBi | P60785 |
Family and domain databases
CDDi | cd03709, lepA_C, 1 hit |
Gene3Di | 3.30.70.2570, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_00071, LepA, 1 hit |
InterProi | View protein in InterPro IPR006297, EF-4 IPR035647, EFG_III/V IPR000640, EFG_V-like IPR004161, EFTu-like_2 IPR031157, G_TR_CS IPR038363, LepA_C_sf IPR013842, LepA_CTD IPR035654, LepA_IV IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR000795, T_Tr_GTP-bd_dom |
PANTHERi | PTHR43512, PTHR43512, 1 hit |
Pfami | View protein in Pfam PF00679, EFG_C, 1 hit PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit PF06421, LepA_C, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF54980, SSF54980, 2 hits |
TIGRFAMsi | TIGR01393, lepA, 1 hit TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P60785-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKNIRNFSII AHIDHGKSTL SDRIIQICGG LSDREMEAQV LDSMDLERER
60 70 80 90 100
GITIKAQSVT LDYKASDGET YQLNFIDTPG HVDFSYEVSR SLAACEGALL
110 120 130 140 150
VVDAGQGVEA QTLANCYTAM EMDLEVVPVL NKIDLPAADP ERVAEEIEDI
160 170 180 190 200
VGIDATDAVR CSAKTGVGVQ DVLERLVRDI PPPEGDPEGP LQALIIDSWF
210 220 230 240 250
DNYLGVVSLI RIKNGTLRKG DKVKVMSTGQ TYNADRLGIF TPKQVDRTEL
260 270 280 290 300
KCGEVGWLVC AIKDIHGAPV GDTLTLARNP AEKALPGFKK VKPQVYAGLF
310 320 330 340 350
PVSSDDYEAF RDALGKLSLN DASLFYEPES SSALGFGFRC GFLGLLHMEI
360 370 380 390 400
IQERLEREYD LDLITTAPTV VYEVETTSRE VIYVDSPSKL PAVNNIYELR
410 420 430 440 450
EPIAECHMLL PQAYLGNVIT LCVEKRGVQT NMVYHGNQVA LTYEIPMAEV
460 470 480 490 500
VLDFFDRLKS TSRGYASLDY NFKRFQASDM VRVDVLINGE RVDALALITH
510 520 530 540 550
RDNSQNRGRE LVEKMKDLIP RQQFDIAIQA AIGTHIIARS TVKQLRKNVL
560 570 580 590
AKCYGGDISR KKKLLQKQKE GKKRMKQIGN VELPQEAFLA ILHVGKDNK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 156 – 185 | TDAVR…PPPEG → HRRGALFSENRRWCAGRSRT SGARHSAAGS (PubMed:2987248).CuratedAdd BLAST | 30 | |
Sequence conflicti | 156 – 185 | TDAVR…PPPEG → HRRGALFSENRRWCAGRSRT SGARHSAAGS (Ref. 2) CuratedAdd BLAST | 30 | |
Sequence conflicti | 266 | Missing (PubMed:2987248).Curated | 1 | |
Sequence conflicti | 266 | Missing (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K00426 Genomic DNA Translation: AAA24063.1 D64044 Genomic DNA Translation: BAA10916.1 U00096 Genomic DNA Translation: AAC75622.1 AP009048 Genomic DNA Translation: BAE76745.1 |
PIRi | H65034, BVECLA |
RefSeqi | NP_417064.1, NC_000913.3 WP_000790168.1, NZ_STEB01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC75622; AAC75622; b2569 BAE76745; BAE76745; BAE76745 |
GeneIDi | 66673542 947051 |
KEGGi | ecj:JW2553 eco:b2569 |
PATRICi | fig|1411691.4.peg.4165 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K00426 Genomic DNA Translation: AAA24063.1 D64044 Genomic DNA Translation: BAA10916.1 U00096 Genomic DNA Translation: AAC75622.1 AP009048 Genomic DNA Translation: BAE76745.1 |
PIRi | H65034, BVECLA |
RefSeqi | NP_417064.1, NC_000913.3 WP_000790168.1, NZ_STEB01000011.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3CB4 | X-ray | 2.80 | A/B/C/D/E/F | 1-599 | [»] | |
3DEG | electron microscopy | - | C | 1-545 | [»] | |
3JCD | electron microscopy | 3.70 | x | 1-599 | [»] | |
3JCE | electron microscopy | 3.20 | x | 1-599 | [»] | |
SMRi | P60785 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260596, 259 interactors 851389, 3 interactors |
DIPi | DIP-29868N |
IntActi | P60785, 62 interactors |
STRINGi | 511145.b2569 |
Proteomic databases
jPOSTi | P60785 |
PaxDbi | P60785 |
PRIDEi | P60785 |
Genome annotation databases
EnsemblBacteriai | AAC75622; AAC75622; b2569 BAE76745; BAE76745; BAE76745 |
GeneIDi | 66673542 947051 |
KEGGi | ecj:JW2553 eco:b2569 |
PATRICi | fig|1411691.4.peg.4165 |
Organism-specific databases
EchoBASEi | EB0524 |
Phylogenomic databases
eggNOGi | COG0481, Bacteria |
HOGENOMi | CLU_009995_3_3_6 |
InParanoidi | P60785 |
PhylomeDBi | P60785 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10529-MONOMER |
BRENDAi | 3.6.5.3, 2026 |
Miscellaneous databases
EvolutionaryTracei | P60785 |
PROi | PR:P60785 |
Family and domain databases
CDDi | cd03709, lepA_C, 1 hit |
Gene3Di | 3.30.70.2570, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_00071, LepA, 1 hit |
InterProi | View protein in InterPro IPR006297, EF-4 IPR035647, EFG_III/V IPR000640, EFG_V-like IPR004161, EFTu-like_2 IPR031157, G_TR_CS IPR038363, LepA_C_sf IPR013842, LepA_CTD IPR035654, LepA_IV IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR000795, T_Tr_GTP-bd_dom |
PANTHERi | PTHR43512, PTHR43512, 1 hit |
Pfami | View protein in Pfam PF00679, EFG_C, 1 hit PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit PF06421, LepA_C, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF54980, SSF54980, 2 hits |
TIGRFAMsi | TIGR01393, lepA, 1 hit TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LEPA_ECOLI | |
Accessioni | P60785Primary (citable) accession number: P60785 Secondary accession number(s): P07682, P76590, Q2MAG1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | April 13, 2004 | |
Last modified: | February 23, 2022 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families