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Protein

Cobrotoxin

Gene
N/A
Organism
Naja atra (Chinese cobra)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to muscle nicotinic acetylcholine receptor (nAChR) and inhibit acetylcholine from binding to the receptor, thereby impairing neuromuscular transmission. Has a higher toxicity than cobrotoxin-b.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei54May be critical for toxicity1
Sitei57May be critical for toxicity1

GO - Molecular functioni

Keywordsi

Molecular functionAcetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Cobrotoxin
Short name:
CBT
Alternative name(s):
Atratoxin
Short neurotoxin 1
OrganismiNaja atra (Chinese cobra)
Taxonomic identifieri8656 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeNaja

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 0.09 mg/kg by subcutaneous injection.

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi59E → Q: Decrease in the rate of isomerization reaction of disulfide bonds. 1 Publication1
Mutagenesisi68K → E: Decrease in the rate of isomerization reaction of disulfide bonds. 1 Publication1
Mutagenesisi68K → Q: Decrease in the rate of isomerization reaction of disulfide bonds. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 214 PublicationsAdd BLAST21
ChainiPRO_000003545222 – 83CobrotoxinAdd BLAST62

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi24 ↔ 45
Disulfide bondi38 ↔ 62
Disulfide bondi64 ↔ 75
Disulfide bondi76 ↔ 81

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP60770

Expressioni

Tissue specificityi

Expressed by the venom gland.Curated

Structurei

Secondary structure

183
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP60770
SMRiP60770
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60770

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006553

Family and domain databases

CDDicd00206 snake_toxin, 1 hit
InterProiView protein in InterPro
IPR003571 Snake_3FTx
IPR018354 Snake_toxin_con_site
IPR035076 Toxin/TOLIP
PfamiView protein in Pfam
PF00087 Toxin_TOLIP, 1 hit
PROSITEiView protein in PROSITE
PS00272 SNAKE_TOXIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60770-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKTLLLTLLV VTIVCLDLGY TLECHNQQSS QTPTTTGCSG GETNCYKKRW
60 70 80
RDHRGYRTER GCGCPSVKNG IEINCCTTDR CNN
Length:83
Mass (Da):9,262
Last modified:April 13, 2004 - v1
Checksum:i4DD6077C92717052
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2K → E in AAG43384 (Ref. 4) Curated1
Sequence conflicti52D → Y in CAB43097 (PubMed:10512733).Curated1
Sequence conflicti66S → I in AAG43384 (Ref. 4) Curated1
Sequence conflicti73I → S in AAG43384 (Ref. 4) Curated1
Sequence conflicti79 – 80DR → G in CAB43097 (PubMed:10512733).Curated2

Mass spectrometryi

Molecular mass is 6952 Da from positions 22 - 83. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58519 mRNA Translation: AAB03221.1
U58520 mRNA Translation: AAB03222.1
U58521 mRNA Translation: AAB03223.1
U77490 mRNA Translation: AAB36930.1
U77491 mRNA Translation: AAB36931.1
U77492 mRNA Translation: AAB36932.1
U42582 mRNA Translation: AAB01538.1
Y12492 Genomic DNA Translation: CAA73097.2
AF161797 Genomic DNA Translation: AAG43384.1
AJ239050 mRNA Translation: CAB43097.1
AY471578 mRNA Translation: AAR33035.1
PIRiJC5769 N1NJ1F

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58519 mRNA Translation: AAB03221.1
U58520 mRNA Translation: AAB03222.1
U58521 mRNA Translation: AAB03223.1
U77490 mRNA Translation: AAB36930.1
U77491 mRNA Translation: AAB36931.1
U77492 mRNA Translation: AAB36932.1
U42582 mRNA Translation: AAB01538.1
Y12492 Genomic DNA Translation: CAA73097.2
AF161797 Genomic DNA Translation: AAG43384.1
AJ239050 mRNA Translation: CAB43097.1
AY471578 mRNA Translation: AAR33035.1
PIRiJC5769 N1NJ1F

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1COENMR-A22-83[»]
1V6PX-ray0.87A/B22-83[»]
ProteinModelPortaliP60770
SMRiP60770
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP60770

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG006553

Miscellaneous databases

EvolutionaryTraceiP60770

Family and domain databases

CDDicd00206 snake_toxin, 1 hit
InterProiView protein in InterPro
IPR003571 Snake_3FTx
IPR018354 Snake_toxin_con_site
IPR035076 Toxin/TOLIP
PfamiView protein in Pfam
PF00087 Toxin_TOLIP, 1 hit
PROSITEiView protein in PROSITE
PS00272 SNAKE_TOXIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei3S1CB_NAJAT
AccessioniPrimary (citable) accession number: P60770
Secondary accession number(s): O13079
, P01430, Q675L7, Q9DE57, Q9W6X0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 13, 2004
Last modified: November 22, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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