UniProtKB - P60764 (RAC3_MOUSE)
Protein
Ras-related C3 botulinum toxin substrate 3
Gene
Rac3
Organism
Mus musculus (Mouse)
Status
Functioni
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner.By similarity
Catalytic activityi
Activity regulationi
Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase. Regulated by the GEF protein DOCK7.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 17 | GTPBy similarity | 8 | |
Nucleotide bindingi | 57 – 61 | GTPBy similarity | 5 | |
Nucleotide bindingi | 115 – 118 | GTPBy similarity | 4 |
GO - Molecular functioni
- calcium-dependent protein binding Source: MGI
- GTPase activity Source: UniProtKB
- GTP binding Source: GO_Central
- protein kinase binding Source: GO_Central
GO - Biological processi
- actin cytoskeleton organization Source: UniProtKB
- actin filament organization Source: GO_Central
- cell projection assembly Source: UniProtKB
- cerebral cortex GABAergic interneuron development Source: MGI
- cortical cytoskeleton organization Source: GO_Central
- establishment or maintenance of cell polarity Source: GO_Central
- homeostasis of number of cells within a tissue Source: MGI
- neuromuscular process Source: MGI
- neuromuscular process controlling balance Source: MGI
- neuron projection development Source: MGI
- positive regulation of cell adhesion mediated by integrin Source: UniProtKB
- positive regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
- regulation of actin cytoskeleton organization Source: GO_Central
- regulation of cell morphogenesis Source: MGI
- regulation of cell shape Source: GO_Central
- regulation of neuron maturation Source: MGI
- small GTPase mediated signal transduction Source: InterPro
- synaptic transmission, GABAergic Source: MGI
- Wnt signaling pathway Source: MGI
Keywordsi
Molecular function | Hydrolase |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-194840, Rho GTPase cycle R-MMU-4086400, PCP/CE pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Ras-related C3 botulinum toxin substrate 3 (EC:3.6.5.2By similarity)Alternative name(s): p21-Rac3 |
Gene namesi | Name:Rac3Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2180784, Rac3 |
Subcellular locationi
Cytoskeleton
- cytoskeleton By similarity
Plasma membrane
- Cell membrane By similarity
Other locations
- Cytoplasm By similarity
- Endomembrane system By similarity
- lamellipodium By similarity
- perinuclear region By similarity
Note: Membrane-associated when activated. Colocalizes with NRBP to endomembranes and at the cell periphery in lamellipodia. Colocalized with CIB1 in the perinuclear area and at the cell periphery.By similarity
Cytoskeleton
- cytoskeleton Source: MGI
- filamentous actin Source: MGI
Cytosol
- cytosol Source: MGI
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- cell cortex Source: GO_Central
- cell periphery Source: UniProtKB
- cell projection Source: GO_Central
- cytoplasm Source: GO_Central
- cytoplasmic vesicle Source: GO_Central
- endomembrane system Source: UniProtKB
- growth cone Source: MGI
- intracellular membrane-bounded organelle Source: GO_Central
- lamellipodium Source: UniProtKB-SubCell
- neuron projection Source: MGI
- neuronal cell body Source: MGI
- perinuclear region of cytoplasm Source: UniProtKB
- synapse Source: GOC
- vesicle Source: GO_Central
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000198890 | 1 – 189 | Ras-related C3 botulinum toxin substrate 3Add BLAST | 189 | |
PropeptideiPRO_0000281241 | 190 – 192 | Removed in mature formBy similarity | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 189 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 189 | S-geranylgeranyl cysteineBy similarity | 1 |
Keywords - PTMi
Lipoprotein, Methylation, PrenylationProteomic databases
jPOSTi | P60764 |
PaxDbi | P60764 |
PeptideAtlasi | P60764 |
PRIDEi | P60764 |
PTM databases
iPTMneti | P60764 |
PhosphoSitePlusi | P60764 |
SwissPalmi | P60764 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000018012, Expressed in brain and 230 other tissues |
ExpressionAtlasi | P60764, baseline and differential |
Genevisiblei | P60764, MM |
Interactioni
Subunit structurei
Interacts with the GEF protein DOCK7, which promotes the exchange between GDP and GTP, and therefore activates it.
Interacts with C1D.
Interacts (via C-terminal region) with CIB1; the interaction induces their association with the cytoskeleton upon alpha-IIb/beta3 integrin-mediated adhesion.
Interacts with NRBP.
By similarityBinary interactionsi
P60764
With | #Exp. | IntAct |
---|---|---|
Arhgdia [Q99PT1] | 3 | EBI-644949,EBI-494354 |
GO - Molecular functioni
- calcium-dependent protein binding Source: MGI
- protein kinase binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 228420, 2 interactors |
IntActi | P60764, 4 interactors |
MINTi | P60764 |
STRINGi | 10090.ENSMUSP00000018156 |
Miscellaneous databases
RNActi | P60764, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 32 – 40 | Effector regionSequence analysis | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0393, Eukaryota |
GeneTreei | ENSGT00940000153500 |
HOGENOMi | CLU_041217_21_3_1 |
InParanoidi | P60764 |
OMAi | VEGKCHA |
OrthoDBi | 1091615at2759 |
PhylomeDBi | P60764 |
TreeFami | TF101109 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR003578, Small_GTPase_Rho |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51420, RHO, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P60764-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP
60 70 80 90 100
VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE
110 120 130 140 150
VRHHCPHTPI LLVGTKLDLR DDKDTIERLR DKKLAPITYP QGLAMAREIG
160 170 180 190
SVKYLECSAL TQRGLKTVFD EAIRAVLCPP PVKKPGKKCT VF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA2AC13 | A2AC13_MOUSE | Ras-related C3 botulinum toxin subs... | Rac3 | 185 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB040819 mRNA Translation: BAB40573.1 AK089930 mRNA Translation: BAC41001.1 |
CCDSi | CCDS25754.1 |
RefSeqi | NP_573486.1, NM_133223.4 |
Genome annotation databases
Ensembli | ENSMUST00000018156; ENSMUSP00000018156; ENSMUSG00000018012 |
GeneIDi | 170758 |
KEGGi | mmu:170758 |
UCSCi | uc007mug.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB040819 mRNA Translation: BAB40573.1 AK089930 mRNA Translation: BAC41001.1 |
CCDSi | CCDS25754.1 |
RefSeqi | NP_573486.1, NM_133223.4 |
3D structure databases
SMRi | P60764 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 228420, 2 interactors |
IntActi | P60764, 4 interactors |
MINTi | P60764 |
STRINGi | 10090.ENSMUSP00000018156 |
PTM databases
iPTMneti | P60764 |
PhosphoSitePlusi | P60764 |
SwissPalmi | P60764 |
Proteomic databases
jPOSTi | P60764 |
PaxDbi | P60764 |
PeptideAtlasi | P60764 |
PRIDEi | P60764 |
Protocols and materials databases
Antibodypediai | 32958, 81 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000018156; ENSMUSP00000018156; ENSMUSG00000018012 |
GeneIDi | 170758 |
KEGGi | mmu:170758 |
UCSCi | uc007mug.1, mouse |
Organism-specific databases
CTDi | 5881 |
MGIi | MGI:2180784, Rac3 |
Phylogenomic databases
eggNOGi | KOG0393, Eukaryota |
GeneTreei | ENSGT00940000153500 |
HOGENOMi | CLU_041217_21_3_1 |
InParanoidi | P60764 |
OMAi | VEGKCHA |
OrthoDBi | 1091615at2759 |
PhylomeDBi | P60764 |
TreeFami | TF101109 |
Enzyme and pathway databases
Reactomei | R-MMU-194840, Rho GTPase cycle R-MMU-4086400, PCP/CE pathway |
Miscellaneous databases
BioGRID-ORCSi | 170758, 2 hits in 17 CRISPR screens |
PROi | PR:P60764 |
RNActi | P60764, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000018012, Expressed in brain and 230 other tissues |
ExpressionAtlasi | P60764, baseline and differential |
Genevisiblei | P60764, MM |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR003578, Small_GTPase_Rho |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51420, RHO, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAC3_MOUSE | |
Accessioni | P60764Primary (citable) accession number: P60764 Secondary accession number(s): O14658 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2004 |
Last sequence update: | April 13, 2004 | |
Last modified: | February 10, 2021 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families