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Protein

Ras-related C3 botulinum toxin substrate 3

Gene

RAC3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17GTPBy similarity8
Nucleotide bindingi57 – 61GTPBy similarity5
Nucleotide bindingi115 – 118GTPBy similarity4

GO - Molecular functioni

  • calcium-dependent protein binding Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • GTP binding Source: GO_Central
  • protein kinase binding Source: GO_Central

GO - Biological processi

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.5.2 2681
ReactomeiR-HSA-194840 Rho GTPase cycle
R-HSA-4086400 PCP/CE pathway
SIGNORiP60763

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related C3 botulinum toxin substrate 3
Alternative name(s):
p21-Rac3
Gene namesi
Name:RAC3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000169750.8
HGNCiHGNC:9803 RAC3
MIMi602050 gene
neXtProtiNX_P60763

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi5881
OpenTargetsiENSG00000169750
PharmGKBiPA34164

Chemistry databases

ChEMBLiCHEMBL6087

Polymorphism and mutation databases

BioMutaiRAC3
DMDMi46397673

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001988891 – 189Ras-related C3 botulinum toxin substrate 3Add BLAST189
PropeptideiPRO_0000281240190 – 192Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi32O-linked (GlcNAc) tyrosine; by Photorhabdus PAU_022301 Publication1
Modified residuei189Cysteine methyl esterBy similarity1
Lipidationi189S-geranylgeranyl cysteineBy similarity1

Post-translational modificationi

(Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rac and leads to actin disassembly.1 Publication

Keywords - PTMi

Glycoprotein, Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiP60763
MaxQBiP60763
PaxDbiP60763
PeptideAtlasiP60763
PRIDEiP60763
ProteomicsDBi57225
TopDownProteomicsiP60763

PTM databases

iPTMnetiP60763
PhosphoSitePlusiP60763
SwissPalmiP60763

Expressioni

Tissue specificityi

Highest levels in brain, also detected in heart, placenta and pancreas.

Inductioni

Expression down-regulated in quiescent fibroblasts and clearly induced by serum stimulation.

Gene expression databases

BgeeiENSG00000169750 Expressed in 111 organ(s), highest expression level in cerebellar hemisphere
CleanExiHS_RAC3
ExpressionAtlasiP60763 baseline and differential
GenevisibleiP60763 HS

Organism-specific databases

HPAiHPA047820

Interactioni

Subunit structurei

Interacts with the GEF protein DOCK7, which promotes the exchange between GDP and GTP, and therefore activates it. Interacts with C1D. Interacts (via C-terminal region) with CIB1; the interaction induces their association with the cytoskeleton upon alpha-IIb/beta3 integrin-mediated adhesion.4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111819, 17 interactors
DIPiDIP-33881N
IntActiP60763, 24 interactors
MINTiP60763
STRINGi9606.ENSP00000304283

Structurei

Secondary structure

1192
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP60763
SMRiP60763
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60763

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector regionSequence analysis9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000118978
HOGENOMiHOG000233974
HOVERGENiHBG009351
InParanoidiP60763
KOiK07861
OMAiGKKCTMF
OrthoDBiEOG091G0KCM
PhylomeDBiP60763
TreeFamiTF101109

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P60763-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP
60 70 80 90 100
VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE
110 120 130 140 150
VRHHCPHTPI LLVGTKLDLR DDKDTIERLR DKKLAPITYP QGLAMAREIG
160 170 180 190
SVKYLECSAL TQRGLKTVFD EAIRAVLCPP PVKKPGKKCT VF
Length:192
Mass (Da):21,379
Last modified:April 13, 2004 - v1
Checksum:i560BBC26BB7CDF4A
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3QLK0J3QLK0_HUMAN
Ras-related C3 botulinum toxin subs...
RAC3
136Annotation score:
J3KSC4J3KSC4_HUMAN
Ras-related C3 botulinum toxin subs...
RAC3
130Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008591 mRNA Translation: AAC51667.1
AF498966 mRNA Translation: AAM21113.1
BT019443 mRNA Translation: AAV38250.1
BC009605 mRNA Translation: AAH09605.1
BC015197 mRNA Translation: AAH15197.1
CCDSiCCDS11798.1
RefSeqiNP_005043.1, NM_005052.2
UniGeneiHs.45002

Genome annotation databases

EnsembliENST00000306897; ENSP00000304283; ENSG00000169750
GeneIDi5881
KEGGihsa:5881
UCSCiuc002kdf.4 human

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008591 mRNA Translation: AAC51667.1
AF498966 mRNA Translation: AAM21113.1
BT019443 mRNA Translation: AAV38250.1
BC009605 mRNA Translation: AAH09605.1
BC015197 mRNA Translation: AAH15197.1
CCDSiCCDS11798.1
RefSeqiNP_005043.1, NM_005052.2
UniGeneiHs.45002

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C2HX-ray1.85A/B1-192[»]
2G0NX-ray1.90A/B1-177[»]
2IC5X-ray1.90A/B1-178[»]
2OV2X-ray2.10A/B/C/D/E/F/G/H1-177[»]
2QMEX-ray1.75A1-177[»]
ProteinModelPortaliP60763
SMRiP60763
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111819, 17 interactors
DIPiDIP-33881N
IntActiP60763, 24 interactors
MINTiP60763
STRINGi9606.ENSP00000304283

Chemistry databases

ChEMBLiCHEMBL6087

PTM databases

iPTMnetiP60763
PhosphoSitePlusiP60763
SwissPalmiP60763

Polymorphism and mutation databases

BioMutaiRAC3
DMDMi46397673

Proteomic databases

EPDiP60763
MaxQBiP60763
PaxDbiP60763
PeptideAtlasiP60763
PRIDEiP60763
ProteomicsDBi57225
TopDownProteomicsiP60763

Protocols and materials databases

DNASUi5881
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000306897; ENSP00000304283; ENSG00000169750
GeneIDi5881
KEGGihsa:5881
UCSCiuc002kdf.4 human

Organism-specific databases

CTDi5881
DisGeNETi5881
EuPathDBiHostDB:ENSG00000169750.8
GeneCardsiRAC3
HGNCiHGNC:9803 RAC3
HPAiHPA047820
MIMi602050 gene
neXtProtiNX_P60763
OpenTargetsiENSG00000169750
PharmGKBiPA34164
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000118978
HOGENOMiHOG000233974
HOVERGENiHBG009351
InParanoidiP60763
KOiK07861
OMAiGKKCTMF
OrthoDBiEOG091G0KCM
PhylomeDBiP60763
TreeFamiTF101109

Enzyme and pathway databases

BRENDAi3.6.5.2 2681
ReactomeiR-HSA-194840 Rho GTPase cycle
R-HSA-4086400 PCP/CE pathway
SIGNORiP60763

Miscellaneous databases

EvolutionaryTraceiP60763
GeneWikiiRAC3
GenomeRNAii5881
PROiPR:P60763
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000169750 Expressed in 111 organ(s), highest expression level in cerebellar hemisphere
CleanExiHS_RAC3
ExpressionAtlasiP60763 baseline and differential
GenevisibleiP60763 HS

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAC3_HUMAN
AccessioniPrimary (citable) accession number: P60763
Secondary accession number(s): O14658, Q5U0M8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: November 7, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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