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UniProtKB - P60757 (HIS1_ECOLI)
Protein
ATP phosphoribosyltransferase
Gene
hisG
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.
1 PublicationCatalytic activityi
- EC:2.4.2.171 Publication
Cofactori
Mg2+1 Publication
Activity regulationi
Feedback inhibited by histidine. Also inhibited by AMP and PR-ATP.2 Publications
: L-histidine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.1 Publication This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP phosphoribosyltransferase activity Source: EcoCyc
- magnesium ion binding Source: UniProtKB-UniRule
GO - Biological processi
- histidine biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Amino-acid biosynthesis, Histidine biosynthesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:ATPPHOSRIBOSTRANS-MONOMER |
BRENDAi | 2.4.2.17, 2026 |
UniPathwayi | UPA00031;UER00006 |
Names & Taxonomyi
Protein namesi | Recommended name: ATP phosphoribosyltransferase (EC:2.4.2.17)Short name: ATP-PRT Short name: ATP-PRTase |
Gene namesi | Name:hisG Ordered Locus Names:b2019, JW2001 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000151848 | 1 – 299 | ATP phosphoribosyltransferaseAdd BLAST | 299 |
Proteomic databases
jPOSTi | P60757 |
PaxDbi | P60757 |
PRIDEi | P60757 |
2D gel databases
SWISS-2DPAGEi | P60757 |
Interactioni
Subunit structurei
Equilibrium between an active dimeric form, an inactive hexameric form and higher aggregates. Interconversion between the various forms is largely reversible and is influenced by the natural substrates and inhibitors of the enzyme.
3 PublicationsProtein-protein interaction databases
BioGRIDi | 4263530, 24 interactors |
IntActi | P60757, 1 interactor |
STRINGi | 511145.b2019 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P60757 |
SMRi | P60757 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P60757 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0040, Bacteria |
HOGENOMi | CLU_038115_1_0_6 |
InParanoidi | P60757 |
OMAi | YVMMDYD |
PhylomeDBi | P60757 |
Family and domain databases
Gene3Di | 3.30.70.120, 1 hit |
HAMAPi | MF_00079, HisG_Long, 1 hit |
InterProi | View protein in InterPro IPR013820, ATP_PRibTrfase_cat IPR018198, ATP_PRibTrfase_CS IPR001348, ATP_PRibTrfase_HisG IPR020621, ATP_PRibTrfase_HisG_long IPR013115, HisG_C IPR011322, N-reg_PII-like_a/b IPR015867, N-reg_PII/ATP_PRibTrfase_C |
PANTHERi | PTHR21403, PTHR21403, 1 hit |
Pfami | View protein in Pfam PF01634, HisG, 1 hit PF08029, HisG_C, 1 hit |
SUPFAMi | SSF54913, SSF54913, 1 hit |
TIGRFAMsi | TIGR00070, hisG, 1 hit TIGR03455, HisG_C-term, 1 hit |
PROSITEi | View protein in PROSITE PS01316, ATP_P_PHORIBOSYLTR, 1 hit |
i Sequence
Sequence statusi: Complete.
P60757-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDNTRLRIA MQKSGRLSDD SRELLARCGI KINLHTQRLI AMAENMPIDI
60 70 80 90 100
LRVRDDDIPG LVMDGVVDLG IIGENVLEEE LLNRRAQGED PRYFTLRRLD
110 120 130 140 150
FGGCRLSLAT PVDEAWDGPL SLNGKRIATS YPHLLKRYLD QKGISFKSCL
160 170 180 190 200
LNGSVEVAPR AGLADAICDL VSTGATLEAN GLREVEVIYR SKACLIQRDG
210 220 230 240 250
EMEESKQQLI DKLLTRIQGV IQARESKYIM MHAPTERLDE VIALLPGAER
260 270 280 290
PTILPLAGDQ QRVAMHMVSS ETLFWETMEK LKALGASSIL VLPIEKMME
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 6 | R → P in CAA23549 (PubMed:6170941).Curated | 1 | |
Sequence conflicti | 49 – 50 | DI → GV in CAA31811 (PubMed:3062174).Curated | 2 | |
Sequence conflicti | 263 | V → A in CAA31811 (PubMed:3062174).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13462 Genomic DNA Translation: CAA31811.1 X63697 Genomic DNA Translation: CAA45224.1 U02070 Unassigned DNA Translation: AAA19742.1 U00096 Genomic DNA Translation: AAC75080.1 AP009048 Genomic DNA Translation: BAA15850.1 V00284 Genomic DNA Translation: CAA23549.1 |
PIRi | B64967, XREC |
RefSeqi | NP_416523.1, NC_000913.3 WP_000131782.1, NZ_STEB01000048.1 |
Genome annotation databases
EnsemblBacteriai | AAC75080; AAC75080; b2019 BAA15850; BAA15850; BAA15850 |
GeneIDi | 66674083 946549 |
KEGGi | ecj:JW2001 eco:b2019 |
PATRICi | fig|1411691.4.peg.233 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13462 Genomic DNA Translation: CAA31811.1 X63697 Genomic DNA Translation: CAA45224.1 U02070 Unassigned DNA Translation: AAA19742.1 U00096 Genomic DNA Translation: AAC75080.1 AP009048 Genomic DNA Translation: BAA15850.1 V00284 Genomic DNA Translation: CAA23549.1 |
PIRi | B64967, XREC |
RefSeqi | NP_416523.1, NC_000913.3 WP_000131782.1, NZ_STEB01000048.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1H3D | X-ray | 2.70 | A | 1-299 | [»] | |
1Q1K | X-ray | 2.90 | A | 1-299 | [»] | |
AlphaFoldDBi | P60757 | |||||
SMRi | P60757 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263530, 24 interactors |
IntActi | P60757, 1 interactor |
STRINGi | 511145.b2019 |
Chemistry databases
DrugBanki | DB01661, 1-(5-phospho-D-ribosyl)-ATP |
2D gel databases
SWISS-2DPAGEi | P60757 |
Proteomic databases
jPOSTi | P60757 |
PaxDbi | P60757 |
PRIDEi | P60757 |
Genome annotation databases
EnsemblBacteriai | AAC75080; AAC75080; b2019 BAA15850; BAA15850; BAA15850 |
GeneIDi | 66674083 946549 |
KEGGi | ecj:JW2001 eco:b2019 |
PATRICi | fig|1411691.4.peg.233 |
Organism-specific databases
EchoBASEi | EB0444 |
Phylogenomic databases
eggNOGi | COG0040, Bacteria |
HOGENOMi | CLU_038115_1_0_6 |
InParanoidi | P60757 |
OMAi | YVMMDYD |
PhylomeDBi | P60757 |
Enzyme and pathway databases
UniPathwayi | UPA00031;UER00006 |
BioCyci | EcoCyc:ATPPHOSRIBOSTRANS-MONOMER |
BRENDAi | 2.4.2.17, 2026 |
Miscellaneous databases
EvolutionaryTracei | P60757 |
PROi | PR:P60757 |
Family and domain databases
Gene3Di | 3.30.70.120, 1 hit |
HAMAPi | MF_00079, HisG_Long, 1 hit |
InterProi | View protein in InterPro IPR013820, ATP_PRibTrfase_cat IPR018198, ATP_PRibTrfase_CS IPR001348, ATP_PRibTrfase_HisG IPR020621, ATP_PRibTrfase_HisG_long IPR013115, HisG_C IPR011322, N-reg_PII-like_a/b IPR015867, N-reg_PII/ATP_PRibTrfase_C |
PANTHERi | PTHR21403, PTHR21403, 1 hit |
Pfami | View protein in Pfam PF01634, HisG, 1 hit PF08029, HisG_C, 1 hit |
SUPFAMi | SSF54913, SSF54913, 1 hit |
TIGRFAMsi | TIGR00070, hisG, 1 hit TIGR03455, HisG_C-term, 1 hit |
PROSITEi | View protein in PROSITE PS01316, ATP_P_PHORIBOSYLTR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HIS1_ECOLI | |
Accessioni | P60757Primary (citable) accession number: P60757 Secondary accession number(s): P10366 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2004 |
Last sequence update: | April 13, 2004 | |
Last modified: | May 25, 2022 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families