UniProtKB - P60706 (ACTB_CHICK)
Protein
Actin, cytoplasmic 1
Gene
ACTB
Organism
Gallus gallus (Chicken)
Status
Functioni
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.By similarity
Miscellaneous
In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.By similarity
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- protein kinase binding Source: GO_Central
- structural constituent of postsynaptic actin cytoskeleton Source: GO_Central
GO - Biological processi
- axonogenesis Source: GO_Central
- cell motility Source: GO_Central
- negative regulation of protein binding Source: Ensembl
- synaptic vesicle endocytosis Source: Ensembl
Keywordsi
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-GGA-190873, Gap junction degradation R-GGA-196025, Formation of annular gap junctions R-GGA-2029482, Regulation of actin dynamics for phagocytic cup formation R-GGA-3928662, EPHB-mediated forward signaling R-GGA-3928665, EPH-ephrin mediated repulsion of cells R-GGA-418990, Adherens junctions interactions R-GGA-4420097, VEGFA-VEGFR2 Pathway R-GGA-5626467, RHO GTPases activate IQGAPs R-GGA-5663213, RHO GTPases Activate WASPs and WAVEs R-GGA-5663220, RHO GTPases Activate Formins R-GGA-5689603, UCH proteinases R-GGA-5696394, DNA Damage Recognition in GG-NER R-GGA-8856828, Clathrin-mediated endocytosis |
Names & Taxonomyi
Protein namesi | Recommended name: Actin, cytoplasmic 1Alternative name(s): Beta-actin Cleaved into the following chain: |
Gene namesi | Name:ACTB |
Organismi | Gallus gallus (Chicken) |
Taxonomic identifieri | 9031 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archelosauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galloanserae › Galliformes › Phasianidae › Phasianinae › Gallus |
Proteomesi |
|
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytoskeleton
- cytoskeleton By similarity
Cytoskeleton
- actin cytoskeleton Source: UniProtKB
- actin filament Source: GO_Central
- cortical cytoskeleton Source: Ensembl
- cytoskeleton Source: AgBase
Cytosol
- cytosol Source: Ensembl
Nucleus
- NuA4 histone acetyltransferase complex Source: GO_Central
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: AgBase
Other locations
- axon Source: GO_Central
- cytoplasm Source: AgBase
- dense body Source: AgBase
- focal adhesion Source: AgBase
- lamellipodium Source: AgBase
- membrane Source: GO_Central
- Schaffer collateral - CA1 synapse Source: Ensembl
- synapse Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 73 | H → A: ATP is exchanged at an increased rate; decreased the thermal stability of actin monomers. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000367086 | 1 – 375 | Actin, cytoplasmic 1Add BLAST | 375 | |
Initiator methioninei | Removed; alternate1 Publication | |||
ChainiPRO_0000000789 | 2 – 375 | Actin, cytoplasmic 1, N-terminally processedAdd BLAST | 374 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionine; in Actin, cytoplasmic 1; alternateBy similarity | 1 | |
Modified residuei | 2 | N-acetylaspartate; in Actin, cytoplasmic 1, N-terminally processed1 Publication | 1 | |
Modified residuei | 44 | Methionine (R)-sulfoxideBy similarity | 1 | |
Modified residuei | 47 | Methionine (R)-sulfoxideBy similarity | 1 | |
Modified residuei | 73 | Tele-methylhistidine2 Publications | 1 |
Post-translational modificationi
Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).By similarity
Methylation at His-73 by SETD3 (PubMed:30626964). Methylation stabilizes actin filaments (PubMed:11955010).2 Publications
Keywords - PTMi
Acetylation, Methylation, OxidationProteomic databases
PRIDEi | P60706 |
Expressioni
Gene expression databases
Bgeei | ENSGALG00000009621, Expressed in spleen and 13 other tissues |
ExpressionAtlasi | P60706, baseline and differential |
Interactioni
Subunit structurei
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix (By similarity). Each actin can bind to 4 others (By similarity).
By similarityGO - Molecular functioni
- protein kinase binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 676771, 3 interactors |
DIPi | DIP-41284N |
IntActi | P60706, 1 interactor |
MINTi | P60706 |
STRINGi | 9031.ENSGALP00000015657 |
Family & Domainsi
Sequence similaritiesi
Belongs to the actin family.Curated
Phylogenomic databases
eggNOGi | KOG0676, Eukaryota |
GeneTreei | ENSGT00950000182960 |
HOGENOMi | CLU_027965_0_2_1 |
InParanoidi | P60706 |
OMAi | FHTTAER |
OrthoDBi | 649708at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR004000, Actin IPR020902, Actin/actin-like_CS IPR004001, Actin_CS IPR043129, ATPase_NBD |
PANTHERi | PTHR11937, PTHR11937, 1 hit |
Pfami | View protein in Pfam PF00022, Actin, 1 hit |
PRINTSi | PR00190, ACTIN |
SMARTi | View protein in SMART SM00268, ACTIN, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
PROSITEi | View protein in PROSITE PS00406, ACTINS_1, 1 hit PS00432, ACTINS_2, 1 hit PS01132, ACTINS_ACT_LIKE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P60706-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDDDIAALVV DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK
60 70 80 90 100
DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE
110 120 130 140 150
HPVLLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVL SLYASGRTTG
160 170 180 190 200
IVMDSGDGVT HTVPIYEGYA LPHAILRLDL AGRDLTDYLM KILTERGYSF
210 220 230 240 250
TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI
260 270 280 290 300
GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS
310 320 330 340 350
GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS
360 370
TFQQMWISKQ EYDESGPSIV HRKCF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 336 | K → R in CAA25004 (PubMed:6324080).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00182 Genomic DNA Translation: CAA25004.1 L08165 mRNA Translation: AAA48615.1 |
PIRi | A20888, ATCHB |
RefSeqi | NP_990849.1, NM_205518.1 |
Genome annotation databases
Ensembli | ENSGALT00000015673; ENSGALP00000015657; ENSGALG00000009621 ENSGALT00000089197; ENSGALP00000060844; ENSGALG00000009621 |
GeneIDi | 396526 |
KEGGi | gga:396526 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00182 Genomic DNA Translation: CAA25004.1 L08165 mRNA Translation: AAA48615.1 |
PIRi | A20888, ATCHB |
RefSeqi | NP_990849.1, NM_205518.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3J0S | electron microscopy | 9.00 | A/B/C/D/E/F/G/H/I/J/K/L | 2-375 | [»] | |
SMRi | P60706 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 676771, 3 interactors |
DIPi | DIP-41284N |
IntActi | P60706, 1 interactor |
MINTi | P60706 |
STRINGi | 9031.ENSGALP00000015657 |
Proteomic databases
PRIDEi | P60706 |
Genome annotation databases
Ensembli | ENSGALT00000015673; ENSGALP00000015657; ENSGALG00000009621 ENSGALT00000089197; ENSGALP00000060844; ENSGALG00000009621 |
GeneIDi | 396526 |
KEGGi | gga:396526 |
Organism-specific databases
CTDi | 60 |
Phylogenomic databases
eggNOGi | KOG0676, Eukaryota |
GeneTreei | ENSGT00950000182960 |
HOGENOMi | CLU_027965_0_2_1 |
InParanoidi | P60706 |
OMAi | FHTTAER |
OrthoDBi | 649708at2759 |
Enzyme and pathway databases
Reactomei | R-GGA-190873, Gap junction degradation R-GGA-196025, Formation of annular gap junctions R-GGA-2029482, Regulation of actin dynamics for phagocytic cup formation R-GGA-3928662, EPHB-mediated forward signaling R-GGA-3928665, EPH-ephrin mediated repulsion of cells R-GGA-418990, Adherens junctions interactions R-GGA-4420097, VEGFA-VEGFR2 Pathway R-GGA-5626467, RHO GTPases activate IQGAPs R-GGA-5663213, RHO GTPases Activate WASPs and WAVEs R-GGA-5663220, RHO GTPases Activate Formins R-GGA-5689603, UCH proteinases R-GGA-5696394, DNA Damage Recognition in GG-NER R-GGA-8856828, Clathrin-mediated endocytosis |
Miscellaneous databases
PROi | PR:P60706 |
Gene expression databases
Bgeei | ENSGALG00000009621, Expressed in spleen and 13 other tissues |
ExpressionAtlasi | P60706, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR004000, Actin IPR020902, Actin/actin-like_CS IPR004001, Actin_CS IPR043129, ATPase_NBD |
PANTHERi | PTHR11937, PTHR11937, 1 hit |
Pfami | View protein in Pfam PF00022, Actin, 1 hit |
PRINTSi | PR00190, ACTIN |
SMARTi | View protein in SMART SM00268, ACTIN, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
PROSITEi | View protein in PROSITE PS00406, ACTINS_1, 1 hit PS00432, ACTINS_2, 1 hit PS01132, ACTINS_ACT_LIKE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACTB_CHICK | |
Accessioni | P60706Primary (citable) accession number: P60706 Secondary accession number(s): P02570 Q64316 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | April 1, 1988 | |
Last modified: | April 7, 2021 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families