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Protein

Nuclear protein localization protein 4 homolog

Gene

Nploc4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and UFD1, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I (By similarity).By similarity

Pathwayi: proteasomal ubiquitin-dependent pathway

This protein is involved in the pathway proteasomal ubiquitin-dependent pathway, which is part of Protein degradation.
View all proteins of this organism that are known to be involved in the pathway proteasomal ubiquitin-dependent pathway and in Protein degradation.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri580 – 608RanBP2-typePROSITE-ProRule annotationAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-110320 Translesion Synthesis by POLH
UniPathwayi
UPA00144

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear protein localization protein 4 homolog
Short name:
Protein NPL4
Gene namesi
Name:Nploc4
Synonyms:Kiaa1499, Npl4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2679787 Nploc4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000579422 – 608Nuclear protein localization protein 4 homologAdd BLAST607

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei179N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP60670
MaxQBiP60670
PaxDbiP60670
PeptideAtlasiP60670
PRIDEiP60670

PTM databases

iPTMnetiP60670
PhosphoSitePlusiP60670

Expressioni

Gene expression databases

BgeeiENSMUSG00000039703 Expressed in 271 organ(s), highest expression level in cumulus cell
GenevisibleiP60670 MM

Interactioni

Subunit structurei

Heterodimer with UFD1. The heterodimer binds ubiquitinated proteins. The heterodimer binds to VCP and inhibits Golgi membrane fusion (By similarity). Interacts with ZFAND2B; probably through VCP (PubMed:24160817).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229901, 4 interactors
ComplexPortaliCPX-136 Vcp-Npl4-Ufd1 AAA ATPase complex
CORUMiP60670
IntActiP60670, 3 interactors
STRINGi10090.ENSMUSP00000035851

Structurei

Secondary structure

1608
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP60670
SMRiP60670
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60670

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini226 – 363MPNPROSITE-ProRule annotationAdd BLAST138

Domaini

Binds ubiquitinated proteins via its RanBP2-type zinc finger.By similarity

Sequence similaritiesi

Belongs to the NPL4 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri580 – 608RanBP2-typePROSITE-ProRule annotationAdd BLAST29

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2834 Eukaryota
COG5100 LUCA
GeneTreeiENSGT00390000018731
HOGENOMiHOG000008139
HOVERGENiHBG052659
InParanoidiP60670
KOiK14015
OMAiKIKHGDM
OrthoDBiEOG091G0Q00
PhylomeDBiP60670
TreeFamiTF314173

Family and domain databases

CDDicd08061 MPN_NPL4, 1 hit
InterProiView protein in InterPro
IPR037518 MPN
IPR016563 Npl4
IPR007717 NPL4_C
IPR024682 Npl4_Ub-like_dom
IPR007716 NPL4_Zn-bd_put
IPR029071 Ubiquitin-like_domsf
IPR001876 Znf_RanBP2
IPR036443 Znf_RanBP2_sf
PfamiView protein in Pfam
PF05021 NPL4, 1 hit
PF11543 UN_NPL4, 1 hit
PF05020 zf-NPL4, 1 hit
PIRSFiPIRSF010052 Polyub_prc_Npl4, 1 hit
SMARTiView protein in SMART
SM00547 ZnF_RBZ, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
SSF90209 SSF90209, 1 hit
PROSITEiView protein in PROSITE
PS50249 MPN, 1 hit
PS01358 ZF_RANBP2_1, 1 hit
PS50199 ZF_RANBP2_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: P60670-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAESIIIRVQ SPDGVKRITA TKRETAATFL KKVAKEFGFQ NNGFSVYINR
60 70 80 90 100
NKTGEITASS SKSLHLLKIK HGDLLFLFPS SLAGPSSEME TSTSVGLKAF
110 120 130 140 150
GAPNVVEDEI DQYLSKQDGK IYRSRDPQLC RHGPLGKCVH CVPLEPFDED
160 170 180 190 200
YLNHLEPPVK HMSFHAYIRK LTGGADKGKF VALENISCKI KSGCEGHLPW
210 220 230 240 250
PNGICTKCQP SAITLNRQKY RHVDNIMFEN HTVADRFLDF WRKTGNQHFG
260 270 280 290 300
YLYGRYTEHK DIPLGIRAEV AAIYEPPQIG TQNSLELLED PKAEVVDEIA
310 320 330 340 350
AKLGLRKVGW IFTDLVSEDT RKGTVRYSRN KDTYFLSSEE CITAGDFQNK
360 370 380 390 400
HPNICRLSPD GHFGSKFVTA VATGGPDNQV HFEGYQVSNQ CMALVRDECL
410 420 430 440 450
LPCKDAPELG YAKESSSEQY VPDVFYKDID KFGNEITQLA RPLPVEYLII
460 470 480 490 500
DITTTFPKDP VYTFSISQNP FPIENRDVLG ETQDFHSLAT YLSQNTSSVF
510 520 530 540 550
LDTISDFHLL LFLVTNEVMP LQDSISLLLE AVRTRNEELA QTWKKSEQWA
560 570 580 590 600
TIEQLCSTVG VQLPGLHEFG AVGGSARAAT SAMWACQHCT FMNQPGTGHC

EMCSLPRT
Length:608
Mass (Da):68,017
Last modified:January 23, 2007 - v3
Checksum:i378E31B35D559336
GO
Isoform 2 (identifier: P60670-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     452-483: Missing.

Note: No experimental confirmation available.
Show »
Length:576
Mass (Da):64,379
Checksum:i478732FF9B936035
GO

Sequence cautioni

The sequence BAC98185 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009790452 – 483Missing in isoform 2. 1 PublicationAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129375 mRNA Translation: BAC98185.1 Different initiation.
AL669855 Genomic DNA No translation available.
BC065156 mRNA Translation: AAH65156.1
CCDSiCCDS25732.1 [P60670-2]
CCDS56827.1 [P60670-1]
RefSeqiNP_001181952.1, NM_001195023.1 [P60670-1]
NP_955763.1, NM_199469.2 [P60670-2]
UniGeneiMm.309520

Genome annotation databases

EnsembliENSMUST00000044271; ENSMUSP00000035851; ENSMUSG00000039703 [P60670-1]
ENSMUST00000103017; ENSMUSP00000099306; ENSMUSG00000039703 [P60670-2]
GeneIDi217365
KEGGimmu:217365
UCSCiuc007msp.2 mouse [P60670-2]
uc007msq.2 mouse [P60670-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129375 mRNA Translation: BAC98185.1 Different initiation.
AL669855 Genomic DNA No translation available.
BC065156 mRNA Translation: AAH65156.1
CCDSiCCDS25732.1 [P60670-2]
CCDS56827.1 [P60670-1]
RefSeqiNP_001181952.1, NM_001195023.1 [P60670-1]
NP_955763.1, NM_199469.2 [P60670-2]
UniGeneiMm.309520

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PJHNMR-A1-80[»]
ProteinModelPortaliP60670
SMRiP60670
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229901, 4 interactors
ComplexPortaliCPX-136 Vcp-Npl4-Ufd1 AAA ATPase complex
CORUMiP60670
IntActiP60670, 3 interactors
STRINGi10090.ENSMUSP00000035851

PTM databases

iPTMnetiP60670
PhosphoSitePlusiP60670

Proteomic databases

EPDiP60670
MaxQBiP60670
PaxDbiP60670
PeptideAtlasiP60670
PRIDEiP60670

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044271; ENSMUSP00000035851; ENSMUSG00000039703 [P60670-1]
ENSMUST00000103017; ENSMUSP00000099306; ENSMUSG00000039703 [P60670-2]
GeneIDi217365
KEGGimmu:217365
UCSCiuc007msp.2 mouse [P60670-2]
uc007msq.2 mouse [P60670-1]

Organism-specific databases

CTDi55666
MGIiMGI:2679787 Nploc4
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2834 Eukaryota
COG5100 LUCA
GeneTreeiENSGT00390000018731
HOGENOMiHOG000008139
HOVERGENiHBG052659
InParanoidiP60670
KOiK14015
OMAiKIKHGDM
OrthoDBiEOG091G0Q00
PhylomeDBiP60670
TreeFamiTF314173

Enzyme and pathway databases

UniPathwayi
UPA00144

ReactomeiR-MMU-110320 Translesion Synthesis by POLH

Miscellaneous databases

ChiTaRSiNploc4 mouse
EvolutionaryTraceiP60670
PROiPR:P60670
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039703 Expressed in 271 organ(s), highest expression level in cumulus cell
GenevisibleiP60670 MM

Family and domain databases

CDDicd08061 MPN_NPL4, 1 hit
InterProiView protein in InterPro
IPR037518 MPN
IPR016563 Npl4
IPR007717 NPL4_C
IPR024682 Npl4_Ub-like_dom
IPR007716 NPL4_Zn-bd_put
IPR029071 Ubiquitin-like_domsf
IPR001876 Znf_RanBP2
IPR036443 Znf_RanBP2_sf
PfamiView protein in Pfam
PF05021 NPL4, 1 hit
PF11543 UN_NPL4, 1 hit
PF05020 zf-NPL4, 1 hit
PIRSFiPIRSF010052 Polyub_prc_Npl4, 1 hit
SMARTiView protein in SMART
SM00547 ZnF_RBZ, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
SSF90209 SSF90209, 1 hit
PROSITEiView protein in PROSITE
PS50249 MPN, 1 hit
PS01358 ZF_RANBP2_1, 1 hit
PS50199 ZF_RANBP2_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNPL4_MOUSE
AccessioniPrimary (citable) accession number: P60670
Secondary accession number(s): B1ATY4, B1ATY5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 131 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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