UniProtKB - P60670 (NPL4_MOUSE)
Nuclear protein localization protein 4 homolog
Nploc4
Functioni
The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity).
Acts as a negative regulator of type I interferon production via the complex formed with VCP and UFD1, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I (By similarity).
By similarity: proteasomal ubiquitin-dependent pathway Pathwayi
This protein is involved in the pathway proteasomal ubiquitin-dependent pathway, which is part of Protein degradation.View all proteins of this organism that are known to be involved in the pathway proteasomal ubiquitin-dependent pathway and in Protein degradation.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 580 – 608 | RanBP2-typePROSITE-ProRule annotationAdd BLAST | 29 |
GO - Molecular functioni
- ATPase binding Source: MGI
- K48-linked polyubiquitin modification-dependent protein binding Source: Ensembl
- K63-linked polyubiquitin modification-dependent protein binding Source: Ensembl
- metal ion binding Source: UniProtKB-KW
- protein-containing complex binding Source: MGI
- ubiquitin binding Source: MGI
- ubiquitin protein ligase binding Source: MGI
GO - Biological processi
- Golgi organization Source: HGNC-UCL
- negative regulation of RIG-I signaling pathway Source: UniProtKB
- negative regulation of type I interferon production Source: UniProtKB
- retrograde protein transport, ER to cytosol Source: ParkinsonsUK-UCL
- ubiquitin-dependent ERAD pathway Source: HGNC-UCL
- ubiquitin-dependent protein catabolic process Source: UniProtKB
Keywordsi
Molecular function | Chaperone |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-110320, Translesion Synthesis by POLH |
UniPathwayi | UPA00144 |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear protein localization protein 4 homologShort name: Protein NPL4 |
Gene namesi | Name:Nploc4 Synonyms:Kiaa1499, Npl4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2679787, Nploc4 |
VEuPathDBi | HostDB:ENSMUSG00000039703 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Cytoplasm and Cytosol
- cytosol By similarity
Note: Associated with the endoplasmic reticulum and nuclear.By similarity
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum Source: HGNC-UCL
- VCP-NPL4-UFD1 AAA ATPase complex Source: UniProtKB
Nucleus
- nucleus Source: MGI
Other locations
- nuclear outer membrane-endoplasmic reticulum membrane network Source: HGNC-UCL
- UFD1-NPL4 complex Source: ParkinsonsUK-UCL
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000057942 | 2 – 608 | Nuclear protein localization protein 4 homologAdd BLAST | 607 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 179 | N6-acetyllysineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | P60670 |
jPOSTi | P60670 |
MaxQBi | P60670 |
PaxDbi | P60670 |
PeptideAtlasi | P60670 |
PRIDEi | P60670 |
ProteomicsDBi | 295511 [P60670-1] 295512 [P60670-2] |
PTM databases
iPTMneti | P60670 |
PhosphoSitePlusi | P60670 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000039703, Expressed in spermatocyte and 293 other tissues |
Genevisiblei | P60670, MM |
Interactioni
Subunit structurei
Heterodimer with UFD1. The heterodimer binds ubiquitinated proteins. The heterodimer binds to VCP and inhibits Golgi membrane fusion (By similarity).
Interacts with ZFAND2B; probably through VCP (PubMed:24160817).
By similarity1 PublicationGO - Molecular functioni
- ATPase binding Source: MGI
- K48-linked polyubiquitin modification-dependent protein binding Source: Ensembl
- K63-linked polyubiquitin modification-dependent protein binding Source: Ensembl
- ubiquitin binding Source: MGI
- ubiquitin protein ligase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 229901, 36 interactors |
ComplexPortali | CPX-136, Vcp-Npl4-Ufd1 AAA ATPase complex |
CORUMi | P60670 |
IntActi | P60670, 3 interactors |
STRINGi | 10090.ENSMUSP00000099306 |
Miscellaneous databases
RNActi | P60670, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P60670 |
SMRi | P60670 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P60670 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 226 – 363 | MPNPROSITE-ProRule annotationAdd BLAST | 138 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 580 – 608 | RanBP2-typePROSITE-ProRule annotationAdd BLAST | 29 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG2834, Eukaryota |
GeneTreei | ENSGT00390000018731 |
HOGENOMi | CLU_017172_2_0_1 |
InParanoidi | P60670 |
OMAi | KWSRTGR |
PhylomeDBi | P60670 |
TreeFami | TF314173 |
Family and domain databases
CDDi | cd08061, MPN_NPL4, 1 hit |
IDEALi | IID50029 |
InterProi | View protein in InterPro IPR037518, MPN IPR016563, Npl4 IPR007717, NPL4_C IPR024682, Npl4_Ub-like_dom IPR007716, NPL4_Zn-bd_put IPR029071, Ubiquitin-like_domsf IPR001876, Znf_RanBP2 IPR036443, Znf_RanBP2_sf |
PANTHERi | PTHR12710, PTHR12710, 1 hit |
Pfami | View protein in Pfam PF05021, NPL4, 1 hit PF11543, UN_NPL4, 1 hit PF05020, zf-NPL4, 1 hit |
PIRSFi | PIRSF010052, Polyub_prc_Npl4, 1 hit |
SMARTi | View protein in SMART SM00547, ZnF_RBZ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit SSF90209, SSF90209, 1 hit |
PROSITEi | View protein in PROSITE PS50249, MPN, 1 hit PS01358, ZF_RANBP2_1, 1 hit PS50199, ZF_RANBP2_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAESIIIRVQ SPDGVKRITA TKRETAATFL KKVAKEFGFQ NNGFSVYINR
60 70 80 90 100
NKTGEITASS SKSLHLLKIK HGDLLFLFPS SLAGPSSEME TSTSVGLKAF
110 120 130 140 150
GAPNVVEDEI DQYLSKQDGK IYRSRDPQLC RHGPLGKCVH CVPLEPFDED
160 170 180 190 200
YLNHLEPPVK HMSFHAYIRK LTGGADKGKF VALENISCKI KSGCEGHLPW
210 220 230 240 250
PNGICTKCQP SAITLNRQKY RHVDNIMFEN HTVADRFLDF WRKTGNQHFG
260 270 280 290 300
YLYGRYTEHK DIPLGIRAEV AAIYEPPQIG TQNSLELLED PKAEVVDEIA
310 320 330 340 350
AKLGLRKVGW IFTDLVSEDT RKGTVRYSRN KDTYFLSSEE CITAGDFQNK
360 370 380 390 400
HPNICRLSPD GHFGSKFVTA VATGGPDNQV HFEGYQVSNQ CMALVRDECL
410 420 430 440 450
LPCKDAPELG YAKESSSEQY VPDVFYKDID KFGNEITQLA RPLPVEYLII
460 470 480 490 500
DITTTFPKDP VYTFSISQNP FPIENRDVLG ETQDFHSLAT YLSQNTSSVF
510 520 530 540 550
LDTISDFHLL LFLVTNEVMP LQDSISLLLE AVRTRNEELA QTWKKSEQWA
560 570 580 590 600
TIEQLCSTVG VQLPGLHEFG AVGGSARAAT SAMWACQHCT FMNQPGTGHC
EMCSLPRT
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009790 | 452 – 483 | Missing in isoform 2. 1 PublicationAdd BLAST | 32 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK129375 mRNA Translation: BAC98185.1 Different initiation. AL669855 Genomic DNA No translation available. BC065156 mRNA Translation: AAH65156.1 |
CCDSi | CCDS25732.1 [P60670-2] CCDS56827.1 [P60670-1] |
RefSeqi | NP_001181952.1, NM_001195023.1 [P60670-1] NP_955763.1, NM_199469.2 [P60670-2] |
Genome annotation databases
Ensembli | ENSMUST00000044271; ENSMUSP00000035851; ENSMUSG00000039703 [P60670-1] ENSMUST00000103017; ENSMUSP00000099306; ENSMUSG00000039703 [P60670-2] |
GeneIDi | 217365 |
KEGGi | mmu:217365 |
UCSCi | uc007msp.2, mouse [P60670-2] uc007msq.2, mouse [P60670-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK129375 mRNA Translation: BAC98185.1 Different initiation. AL669855 Genomic DNA No translation available. BC065156 mRNA Translation: AAH65156.1 |
CCDSi | CCDS25732.1 [P60670-2] CCDS56827.1 [P60670-1] |
RefSeqi | NP_001181952.1, NM_001195023.1 [P60670-1] NP_955763.1, NM_199469.2 [P60670-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2PJH | NMR | - | A | 1-80 | [»] | |
AlphaFoldDBi | P60670 | |||||
SMRi | P60670 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 229901, 36 interactors |
ComplexPortali | CPX-136, Vcp-Npl4-Ufd1 AAA ATPase complex |
CORUMi | P60670 |
IntActi | P60670, 3 interactors |
STRINGi | 10090.ENSMUSP00000099306 |
PTM databases
iPTMneti | P60670 |
PhosphoSitePlusi | P60670 |
Proteomic databases
EPDi | P60670 |
jPOSTi | P60670 |
MaxQBi | P60670 |
PaxDbi | P60670 |
PeptideAtlasi | P60670 |
PRIDEi | P60670 |
ProteomicsDBi | 295511 [P60670-1] 295512 [P60670-2] |
Protocols and materials databases
Antibodypediai | 10070, 140 antibodies from 28 providers |
DNASUi | 217365 |
Genome annotation databases
Ensembli | ENSMUST00000044271; ENSMUSP00000035851; ENSMUSG00000039703 [P60670-1] ENSMUST00000103017; ENSMUSP00000099306; ENSMUSG00000039703 [P60670-2] |
GeneIDi | 217365 |
KEGGi | mmu:217365 |
UCSCi | uc007msp.2, mouse [P60670-2] uc007msq.2, mouse [P60670-1] |
Organism-specific databases
CTDi | 55666 |
MGIi | MGI:2679787, Nploc4 |
VEuPathDBi | HostDB:ENSMUSG00000039703 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG2834, Eukaryota |
GeneTreei | ENSGT00390000018731 |
HOGENOMi | CLU_017172_2_0_1 |
InParanoidi | P60670 |
OMAi | KWSRTGR |
PhylomeDBi | P60670 |
TreeFami | TF314173 |
Enzyme and pathway databases
UniPathwayi | UPA00144 |
Reactomei | R-MMU-110320, Translesion Synthesis by POLH |
Miscellaneous databases
BioGRID-ORCSi | 217365, 27 hits in 73 CRISPR screens |
ChiTaRSi | Nploc4, mouse |
EvolutionaryTracei | P60670 |
PROi | PR:P60670 |
RNActi | P60670, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039703, Expressed in spermatocyte and 293 other tissues |
Genevisiblei | P60670, MM |
Family and domain databases
CDDi | cd08061, MPN_NPL4, 1 hit |
IDEALi | IID50029 |
InterProi | View protein in InterPro IPR037518, MPN IPR016563, Npl4 IPR007717, NPL4_C IPR024682, Npl4_Ub-like_dom IPR007716, NPL4_Zn-bd_put IPR029071, Ubiquitin-like_domsf IPR001876, Znf_RanBP2 IPR036443, Znf_RanBP2_sf |
PANTHERi | PTHR12710, PTHR12710, 1 hit |
Pfami | View protein in Pfam PF05021, NPL4, 1 hit PF11543, UN_NPL4, 1 hit PF05020, zf-NPL4, 1 hit |
PIRSFi | PIRSF010052, Polyub_prc_Npl4, 1 hit |
SMARTi | View protein in SMART SM00547, ZnF_RBZ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit SSF90209, SSF90209, 1 hit |
PROSITEi | View protein in PROSITE PS50249, MPN, 1 hit PS01358, ZF_RANBP2_1, 1 hit PS50199, ZF_RANBP2_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NPL4_MOUSE | |
Accessioni | P60670Primary (citable) accession number: P60670 Secondary accession number(s): B1ATY4, B1ATY5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 148 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families