UniProtKB - P60664 (HIS6_ECOLI)
Protein
Imidazole glycerol phosphate synthase subunit HisF
Gene
hisF
Organism
Escherichia coli (strain K12)
Status
Functioni
IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.
Catalytic activityi
- 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H+ + L-glutamate1 PublicationEC:4.3.2.101 Publication
Kineticsi
- KM=240 µM for PRFAR1 Publication
- KM=23 µM for NH4Cl1 Publication
pH dependencei
Optimum pH is 8-9.1 Publication
: L-histidine biosynthesis Pathwayi
This protein is involved in step 5 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.Proteins known to be involved in the 9 steps of the subpathway in this organism are:
- ATP phosphoribosyltransferase (hisG), ATP phosphoribosyltransferase (hisG)
- Histidine biosynthesis bifunctional protein HisIE (hisI), Histidine biosynthesis bifunctional protein HisIE (hisI)
- Histidine biosynthesis bifunctional protein HisIE (hisI), Histidine biosynthesis bifunctional protein HisIE (hisI)
- 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA), 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
- Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF), Imidazole glycerol phosphate synthase subunit HisF (hisF), Imidazole glycerol phosphate synthase subunit HisH (hisH)
- Histidine biosynthesis bifunctional protein HisB (hisB), Histidine biosynthesis bifunctional protein HisB (hisB)
- Histidinol-phosphate aminotransferase (hisC), Histidinol-phosphate aminotransferase (hisC)
- Histidine biosynthesis bifunctional protein HisB (hisB), Histidine biosynthesis bifunctional protein HisB (hisB)
- Histidinol dehydrogenase (hisD), Histidinol dehydrogenase (hisD)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 11 | Sequence analysis | 1 | |
Active sitei | 130 | Sequence analysis | 1 |
GO - Molecular functioni
- imidazoleglycerol-phosphate synthase activity Source: EcoCyc
- lyase activity Source: UniProtKB-KW
GO - Biological processi
- histidine biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Lyase |
Biological process | Amino-acid biosynthesis, Histidine biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:CYCLASE-MONOMER MetaCyc:CYCLASE-MONOMER |
BRENDAi | 4.3.1.B2, 2026 |
UniPathwayi | UPA00031;UER00010 |
Names & Taxonomyi
Protein namesi | Recommended name: Imidazole glycerol phosphate synthase subunit HisF (EC:4.3.2.10)Alternative name(s): IGP synthase cyclase subunit IGP synthase subunit HisF ImGP synthase subunit HisF Short name: IGPS subunit HisF |
Gene namesi | Name:hisF Ordered Locus Names:b2025, JW2007 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytosol Source: EcoCyc
- imidazoleglycerol-phosphate synthase complex Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 5 | R → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 5 | R → H: Loss of IGP synthase activity. Weak IGP synthase activity and reduced HisH activity in vitro. 1 Publication | 1 | |
Mutagenesisi | 46 | E → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 46 | E → G: Loss of IGP synthase activity. Weak IGP synthase and HisH activities in vitro. 1 Publication | 1 | |
Mutagenesisi | 123 | Q → A: Decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 123 | Q → R: Loss of IGP synthase activity. Weak HisH activity in vitro. 1 Publication | 1 | |
Mutagenesisi | 124 | C → A: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 124 | C → R: Loss of IGP synthase activity. Weak HisH activity in vitro. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000142156 | 1 – 258 | Imidazole glycerol phosphate synthase subunit HisFAdd BLAST | 258 |
Proteomic databases
jPOSTi | P60664 |
PaxDbi | P60664 |
PRIDEi | P60664 |
Interactioni
Subunit structurei
Heterodimer of HisH and HisF.
1 PublicationBinary interactionsi
P60664
With | #Exp. | IntAct |
---|---|---|
hisH [P60595] | 2 | EBI-9151168,EBI-1126953 |
Protein-protein interaction databases
BioGRIDi | 4259164, 22 interactors 850865, 4 interactors |
ComplexPortali | CPX-5158, Imidazole glycerol phosphate synthase complex |
IntActi | P60664, 5 interactors |
STRINGi | 511145.b2025 |
Family & Domainsi
Sequence similaritiesi
Belongs to the HisA/HisF family.Curated
Phylogenomic databases
eggNOGi | COG0107, Bacteria |
HOGENOMi | CLU_048577_4_0_6 |
InParanoidi | P60664 |
PhylomeDBi | P60664 |
Family and domain databases
CDDi | cd04731, HisF, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_01013, HisF, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR006062, His_biosynth IPR004651, HisF IPR011060, RibuloseP-bd_barrel |
Pfami | View protein in Pfam PF00977, His_biosynth, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR00735, hisF, 1 hit |
i Sequence
Sequence statusi: Complete.
P60664-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLAKRIIPCL DVRDGQVVKG VQFRNHEIIG DIVPLAKRYA EEGADELVFY
60 70 80 90 100
DITASSDGRV VDKSWVSRVA EVIDIPFCVA GGIKSLEDAA KILSFGADKI
110 120 130 140 150
SINSPALADP TLITRLADRF GVQCIVVGID TWYDAETGKY HVNQYTGDES
160 170 180 190 200
RTRVTQWETL DWVQEVQKRG AGEIVLNMMN QDGVRNGYDL EQLKKVREVC
210 220 230 240 250
HVPLIASGGA GTMEHFLEAF RDADVDGALA ASVFHKQIIN IGELKAYLAT
QGVEIRIC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13462 Genomic DNA Translation: CAA31817.1 U00096 Genomic DNA Translation: AAC75086.1 AP009048 Genomic DNA Translation: BAA15857.1 D43637 Genomic DNA Translation: BAA07754.1 |
PIRi | JS0134, OYECHF |
RefSeqi | NP_416529.1, NC_000913.3 WP_000880182.1, NZ_SSUR01000003.1 |
Genome annotation databases
EnsemblBacteriai | AAC75086; AAC75086; b2025 BAA15857; BAA15857; BAA15857 |
GeneIDi | 946516 |
KEGGi | ecj:JW2007 eco:b2025 |
PATRICi | fig|1411691.4.peg.227 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13462 Genomic DNA Translation: CAA31817.1 U00096 Genomic DNA Translation: AAC75086.1 AP009048 Genomic DNA Translation: BAA15857.1 D43637 Genomic DNA Translation: BAA07754.1 |
PIRi | JS0134, OYECHF |
RefSeqi | NP_416529.1, NC_000913.3 WP_000880182.1, NZ_SSUR01000003.1 |
3D structure databases
SMRi | P60664 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259164, 22 interactors 850865, 4 interactors |
ComplexPortali | CPX-5158, Imidazole glycerol phosphate synthase complex |
IntActi | P60664, 5 interactors |
STRINGi | 511145.b2025 |
Proteomic databases
jPOSTi | P60664 |
PaxDbi | P60664 |
PRIDEi | P60664 |
Genome annotation databases
EnsemblBacteriai | AAC75086; AAC75086; b2025 BAA15857; BAA15857; BAA15857 |
GeneIDi | 946516 |
KEGGi | ecj:JW2007 eco:b2025 |
PATRICi | fig|1411691.4.peg.227 |
Organism-specific databases
EchoBASEi | EB0443 |
Phylogenomic databases
eggNOGi | COG0107, Bacteria |
HOGENOMi | CLU_048577_4_0_6 |
InParanoidi | P60664 |
PhylomeDBi | P60664 |
Enzyme and pathway databases
UniPathwayi | UPA00031;UER00010 |
BioCyci | EcoCyc:CYCLASE-MONOMER MetaCyc:CYCLASE-MONOMER |
BRENDAi | 4.3.1.B2, 2026 |
Miscellaneous databases
PROi | PR:P60664 |
Family and domain databases
CDDi | cd04731, HisF, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_01013, HisF, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR006062, His_biosynth IPR004651, HisF IPR011060, RibuloseP-bd_barrel |
Pfami | View protein in Pfam PF00977, His_biosynth, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR00735, hisF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HIS6_ECOLI | |
Accessioni | P60664Primary (citable) accession number: P60664 Secondary accession number(s): P10373 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2004 |
Last sequence update: | March 29, 2004 | |
Last modified: | December 2, 2020 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families