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UniProtKB - P60584 (CAIA_ECOLI)
Protein
Crotonobetainyl-CoA reductase
Gene
caiA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA (PubMed:8060125, PubMed:10209289, PubMed:10978161).
The electron donor could be the FixA/FixB complex (PubMed:12081978).
2 Publications2 PublicationsCatalytic activityi
- γ-butyrobetainyl-CoA + H+ + oxidized [electron-transfer flavoprotein] = crotonobetainyl-CoA + reduced [electron-transfer flavoprotein]UniRule annotation1 PublicationEC:1.3.8.13UniRule annotation1 Publication
Cofactori
FADUniRule annotation1 PublicationNote: Binds 1 FAD per subunit.1 Publication
pH dependencei
Optimum pH is 7.8.1 Publication
Temperature dependencei
Optimum temperature is 40-45 degrees Celsius.1 Publication
: carnitine metabolism Pathwayi
This protein is involved in the pathway carnitine metabolism, which is part of Amine and polyamine metabolism.UniRule annotationCuratedView all proteins of this organism that are known to be involved in the pathway carnitine metabolism and in Amine and polyamine metabolism.
GO - Molecular functioni
- acyl-CoA dehydrogenase activity Source: InterPro
- flavin adenine dinucleotide binding Source: InterPro
- oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor Source: UniProtKB-UniRule
GO - Biological processi
- carnitine metabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
BioCyci | EcoCyc:CROBETREDUCT-MONOMER |
BRENDAi | 1.3.8.13, 2026 |
UniPathwayi | UPA00117 |
Names & Taxonomyi
Protein namesi | Recommended name: Crotonobetainyl-CoA reductaseUniRule annotation (EC:1.3.8.13UniRule annotation1 Publication)Alternative name(s): Crotobetaine reductase1 Publication Crotonobetainyl-CoA dehydrogenaseUniRule annotation |
Gene namesi | Name:caiA1 PublicationUniRule annotation Synonyms:yaaO Ordered Locus Names:b0039, JW0038 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation1 Publication
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Deletion of the gene abolishes gamma-butyrobetaine formation and increases L-carnitine production under anaerobic and aerobic conditions.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201193 | 1 – 380 | Crotonobetainyl-CoA reductaseAdd BLAST | 380 |
Proteomic databases
PaxDbi | P60584 |
PRIDEi | P60584 |
Expressioni
Inductioni
Transcribed during anaerobic growth in the presence of L-carnitine or crotonobetaine.2 Publications
Interactioni
Subunit structurei
Homotetramer.
UniRule annotation1 PublicationProtein-protein interaction databases
BioGRIDi | 4263349, 362 interactors |
DIPi | DIP-9241N |
IntActi | P60584, 5 interactors |
STRINGi | 511145.b0039 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1960, Bacteria |
HOGENOMi | CLU_018204_0_2_6 |
InParanoidi | P60584 |
PhylomeDBi | P60584 |
Family and domain databases
Gene3Di | 1.10.540.10, 1 hit |
HAMAPi | MF_01052, CaiA, 1 hit |
InterProi | View protein in InterPro IPR006089, Acyl-CoA_DH_CS IPR006091, Acyl-CoA_Oxase/DH_cen-dom IPR036250, AcylCo_DH-like_C IPR009075, AcylCo_DH/oxidase_C IPR013786, AcylCoA_DH/ox_N IPR037069, AcylCoA_DH/ox_N_sf IPR009100, AcylCoA_DH/oxidase_NM_dom IPR023450, CaiA |
Pfami | View protein in Pfam PF00441, Acyl-CoA_dh_1, 1 hit PF02770, Acyl-CoA_dh_M, 1 hit PF02771, Acyl-CoA_dh_N, 1 hit |
SUPFAMi | SSF47203, SSF47203, 1 hit SSF56645, SSF56645, 1 hit |
PROSITEi | View protein in PROSITE PS00072, ACYL_COA_DH_1, 1 hit PS00073, ACYL_COA_DH_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P60584-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDFNLNDEQE LFVAGIRELM ASENWEAYFA ECDRDSVYPE RFVKALADMG
60 70 80 90 100
IDSLLIPEEH GGLDAGFVTL AAVWMELGRL GAPTYVLYQL PGGFNTFLRE
110 120 130 140 150
GTQEQIDKIM AFRGTGKQMW NSAITEPGAG SDVGSLKTTY TRRNGKIYLN
160 170 180 190 200
GSKCFITSSA YTPYIVVMAR DGASPDKPVY TEWFVDMSKP GIKVTKLEKL
210 220 230 240 250
GLRMDSCCEI TFDDVELDEK DMFGREGNGF NRVKEEFDHE RFLVALTNYG
260 270 280 290 300
TAMCAFEDAA RYANQRVQFG EAIGRFQLIQ EKFAHMAIKL NSMKNMLYEA
310 320 330 340 350
AWKADNGTIT SGDAAMCKYF CANAAFEVVD SAMQVLGGVG IAGNHRISRF
360 370 380
WRDLRVDRVS GGSDEMQILT LGRAVLKQYR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X73904 Genomic DNA Translation: CAA52111.1 U00096 Genomic DNA Translation: AAC73150.1 AP009048 Genomic DNA Translation: BAB96608.2 |
PIRi | G64724, I41011 |
RefSeqi | NP_414581.1, NC_000913.3 WP_000347117.1, NZ_STEB01000010.1 |
Genome annotation databases
EnsemblBacteriai | AAC73150; AAC73150; b0039 BAB96608; BAB96608; BAB96608 |
GeneIDi | 67416114 949064 |
KEGGi | ecj:JW0038 eco:b0039 |
PATRICi | fig|1411691.4.peg.2244 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X73904 Genomic DNA Translation: CAA52111.1 U00096 Genomic DNA Translation: AAC73150.1 AP009048 Genomic DNA Translation: BAB96608.2 |
PIRi | G64724, I41011 |
RefSeqi | NP_414581.1, NC_000913.3 WP_000347117.1, NZ_STEB01000010.1 |
3D structure databases
SMRi | P60584 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263349, 362 interactors |
DIPi | DIP-9241N |
IntActi | P60584, 5 interactors |
STRINGi | 511145.b0039 |
Proteomic databases
PaxDbi | P60584 |
PRIDEi | P60584 |
Genome annotation databases
EnsemblBacteriai | AAC73150; AAC73150; b0039 BAB96608; BAB96608; BAB96608 |
GeneIDi | 67416114 949064 |
KEGGi | ecj:JW0038 eco:b0039 |
PATRICi | fig|1411691.4.peg.2244 |
Organism-specific databases
EchoBASEi | EB1521 |
Phylogenomic databases
eggNOGi | COG1960, Bacteria |
HOGENOMi | CLU_018204_0_2_6 |
InParanoidi | P60584 |
PhylomeDBi | P60584 |
Enzyme and pathway databases
UniPathwayi | UPA00117 |
BioCyci | EcoCyc:CROBETREDUCT-MONOMER |
BRENDAi | 1.3.8.13, 2026 |
Miscellaneous databases
PROi | PR:P60584 |
Family and domain databases
Gene3Di | 1.10.540.10, 1 hit |
HAMAPi | MF_01052, CaiA, 1 hit |
InterProi | View protein in InterPro IPR006089, Acyl-CoA_DH_CS IPR006091, Acyl-CoA_Oxase/DH_cen-dom IPR036250, AcylCo_DH-like_C IPR009075, AcylCo_DH/oxidase_C IPR013786, AcylCoA_DH/ox_N IPR037069, AcylCoA_DH/ox_N_sf IPR009100, AcylCoA_DH/oxidase_NM_dom IPR023450, CaiA |
Pfami | View protein in Pfam PF00441, Acyl-CoA_dh_1, 1 hit PF02770, Acyl-CoA_dh_M, 1 hit PF02771, Acyl-CoA_dh_N, 1 hit |
SUPFAMi | SSF47203, SSF47203, 1 hit SSF56645, SSF56645, 1 hit |
PROSITEi | View protein in PROSITE PS00072, ACYL_COA_DH_1, 1 hit PS00073, ACYL_COA_DH_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CAIA_ECOLI | |
Accessioni | P60584Primary (citable) accession number: P60584 Secondary accession number(s): P31571 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2004 |
Last sequence update: | March 15, 2004 | |
Last modified: | February 23, 2022 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families