UniProtKB - P60546 (KGUA_ECOLI)
Protein
Guanylate kinase
Gene
gmk
Organism
Escherichia coli (strain K12)
Status
Functioni
Essential for recycling GMP and indirectly, cGMP.1 Publication
Catalytic activityi
- EC:2.7.4.8
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 11 – 18 | ATPBy similarity | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- guanylate kinase activity Source: EcoCyc
- identical protein binding Source: EcoCyc
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:GUANYL-KIN-MONOMER MetaCyc:GUANYL-KIN-MONOMER |
BRENDAi | 2.7.4.8, 2026 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:gmk Synonyms:spoR Ordered Locus Names:b3648, JW3623 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000170534 | 1 – 207 | Guanylate kinaseAdd BLAST | 207 |
Proteomic databases
jPOSTi | P60546 |
PaxDbi | P60546 |
PRIDEi | P60546 |
Interactioni
Subunit structurei
Homotetramer (under low ionic conditions) or homodimer (under high ionic conditions).
1 PublicationGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4262568, 25 interactors |
IntActi | P60546, 5 interactors |
STRINGi | 511145.b3648 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P60546 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P60546 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 184 | Guanylate kinase-likeAdd BLAST | 181 |
Sequence similaritiesi
Belongs to the guanylate kinase family.Curated
Phylogenomic databases
eggNOGi | COG0194, Bacteria |
HOGENOMi | CLU_001715_1_0_6 |
InParanoidi | P60546 |
PhylomeDBi | P60546 |
Family and domain databases
HAMAPi | MF_00328, Guanylate_kinase, 1 hit |
InterProi | View protein in InterPro IPR008145, GK/Ca_channel_bsu IPR008144, Guanylate_kin-like_dom IPR017665, Guanylate_kinase IPR020590, Guanylate_kinase_CS IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR03263, guanyl_kin, 1 hit |
PROSITEi | View protein in PROSITE PS00856, GUANYLATE_KINASE_1, 1 hit PS50052, GUANYLATE_KINASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P60546-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQGTLYIVS APSGAGKSSL IQALLKTQPL YDTQVSVSHT TRQPRPGEVH
60 70 80 90 100
GEHYFFVNHD EFKEMISRDA FLEHAEVFGN YYGTSREAIE QVLATGVDVF
110 120 130 140 150
LDIDWQGAQQ IRQKMPHARS IFILPPSKIE LDRRLRGRGQ DSEEVIAKRM
160 170 180 190 200
AQAVAEMSHY AEYDYLIVND DFDTALTDLK TIIRAERLRM SRQKQRHDAL
ISKLLAD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M84400 Genomic DNA Translation: AAB88711.1 L10328 Genomic DNA Translation: AAA62001.1 U00096 Genomic DNA Translation: AAC76672.1 AP009048 Genomic DNA Translation: BAE77645.1 |
PIRi | S43041, KIECGU |
RefSeqi | NP_418105.1, NC_000913.3 WP_001295237.1, NZ_STEB01000024.1 |
Genome annotation databases
EnsemblBacteriai | AAC76672; AAC76672; b3648 BAE77645; BAE77645; BAE77645 |
GeneIDi | 58461629 948163 |
KEGGi | ecj:JW3623 eco:b3648 |
PATRICi | fig|511145.12.peg.3768 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M84400 Genomic DNA Translation: AAB88711.1 L10328 Genomic DNA Translation: AAA62001.1 U00096 Genomic DNA Translation: AAC76672.1 AP009048 Genomic DNA Translation: BAE77645.1 |
PIRi | S43041, KIECGU |
RefSeqi | NP_418105.1, NC_000913.3 WP_001295237.1, NZ_STEB01000024.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1S96 | X-ray | 2.00 | A/B | 1-207 | [»] | |
2AN9 | X-ray | 2.35 | A/B | 1-207 | [»] | |
2ANB | X-ray | 2.90 | A | 1-207 | [»] | |
2ANC | X-ray | 3.20 | A/B/C/D/E/F | 1-207 | [»] | |
2F3R | X-ray | 2.50 | A/B | 1-207 | [»] | |
2F3T | X-ray | 3.16 | A/B/C/D/E/F | 1-207 | [»] | |
SMRi | P60546 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262568, 25 interactors |
IntActi | P60546, 5 interactors |
STRINGi | 511145.b3648 |
Chemistry databases
ChEMBLi | CHEMBL3309016 |
Proteomic databases
jPOSTi | P60546 |
PaxDbi | P60546 |
PRIDEi | P60546 |
Genome annotation databases
EnsemblBacteriai | AAC76672; AAC76672; b3648 BAE77645; BAE77645; BAE77645 |
GeneIDi | 58461629 948163 |
KEGGi | ecj:JW3623 eco:b3648 |
PATRICi | fig|511145.12.peg.3768 |
Organism-specific databases
EchoBASEi | EB0958 |
Phylogenomic databases
eggNOGi | COG0194, Bacteria |
HOGENOMi | CLU_001715_1_0_6 |
InParanoidi | P60546 |
PhylomeDBi | P60546 |
Enzyme and pathway databases
BioCyci | EcoCyc:GUANYL-KIN-MONOMER MetaCyc:GUANYL-KIN-MONOMER |
BRENDAi | 2.7.4.8, 2026 |
Miscellaneous databases
EvolutionaryTracei | P60546 |
PROi | PR:P60546 |
Family and domain databases
HAMAPi | MF_00328, Guanylate_kinase, 1 hit |
InterProi | View protein in InterPro IPR008145, GK/Ca_channel_bsu IPR008144, Guanylate_kin-like_dom IPR017665, Guanylate_kinase IPR020590, Guanylate_kinase_CS IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR03263, guanyl_kin, 1 hit |
PROSITEi | View protein in PROSITE PS00856, GUANYLATE_KINASE_1, 1 hit PS50052, GUANYLATE_KINASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KGUA_ECOLI | |
Accessioni | P60546Primary (citable) accession number: P60546 Secondary accession number(s): P24234, Q2M7W1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2004 |
Last sequence update: | March 15, 2004 | |
Last modified: | April 7, 2021 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families