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Protein

Guanylate kinase

Gene

gmk

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential for recycling GMP and indirectly, cGMP.1 Publication

Catalytic activityi

ATP + GMP = ADP + GDP.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 18ATPBy similarity8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-UniRule
  • guanylate kinase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:GUANYL-KIN-MONOMER
MetaCyc:GUANYL-KIN-MONOMER
BRENDAi2.7.4.8 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate kinase (EC:2.7.4.8)
Alternative name(s):
GMP kinase
Gene namesi
Name:gmk
Synonyms:spoR
Ordered Locus Names:b3648, JW3623
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10965 gmk

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3309016

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001705341 – 207Guanylate kinaseAdd BLAST207

Proteomic databases

EPDiP60546
PaxDbiP60546
PRIDEiP60546

Interactioni

Subunit structurei

Homotetramer (under low ionic conditions) or homodimer (under high ionic conditions).1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4262568, 25 interactors
IntActiP60546, 5 interactors
STRINGi316385.ECDH10B_3830

Structurei

Secondary structure

1207
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP60546
SMRiP60546
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60546

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 184Guanylate kinase-likeAdd BLAST181

Sequence similaritiesi

Belongs to the guanylate kinase family.Curated

Phylogenomic databases

eggNOGiENOG4108UHA Bacteria
COG0194 LUCA
HOGENOMiHOG000037638
InParanoidiP60546
KOiK00942
PhylomeDBiP60546

Family and domain databases

HAMAPiMF_00328 Guanylate_kinase, 1 hit
InterProiView protein in InterPro
IPR008145 GK/Ca_channel_bsu
IPR008144 Guanylate_kin-like_dom
IPR017665 Guanylate_kinase
IPR020590 Guanylate_kinase_CS
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00625 Guanylate_kin, 1 hit
SMARTiView protein in SMART
SM00072 GuKc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR03263 guanyl_kin, 1 hit
PROSITEiView protein in PROSITE
PS00856 GUANYLATE_KINASE_1, 1 hit
PS50052 GUANYLATE_KINASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P60546-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAQGTLYIVS APSGAGKSSL IQALLKTQPL YDTQVSVSHT TRQPRPGEVH
60 70 80 90 100
GEHYFFVNHD EFKEMISRDA FLEHAEVFGN YYGTSREAIE QVLATGVDVF
110 120 130 140 150
LDIDWQGAQQ IRQKMPHARS IFILPPSKIE LDRRLRGRGQ DSEEVIAKRM
160 170 180 190 200
AQAVAEMSHY AEYDYLIVND DFDTALTDLK TIIRAERLRM SRQKQRHDAL

ISKLLAD
Length:207
Mass (Da):23,593
Last modified:March 15, 2004 - v1
Checksum:i62A99DB4063651E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84400 Genomic DNA Translation: AAB88711.1
L10328 Genomic DNA Translation: AAA62001.1
U00096 Genomic DNA Translation: AAC76672.1
AP009048 Genomic DNA Translation: BAE77645.1
PIRiS43041 KIECGU
RefSeqiNP_418105.1, NC_000913.3
WP_001295237.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76672; AAC76672; b3648
BAE77645; BAE77645; BAE77645
GeneIDi948163
KEGGiecj:JW3623
eco:b3648
PATRICifig|511145.12.peg.3768

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84400 Genomic DNA Translation: AAB88711.1
L10328 Genomic DNA Translation: AAA62001.1
U00096 Genomic DNA Translation: AAC76672.1
AP009048 Genomic DNA Translation: BAE77645.1
PIRiS43041 KIECGU
RefSeqiNP_418105.1, NC_000913.3
WP_001295237.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S96X-ray2.00A/B1-207[»]
2AN9X-ray2.35A/B1-207[»]
2ANBX-ray2.90A1-207[»]
2ANCX-ray3.20A/B/C/D/E/F1-207[»]
2F3RX-ray2.50A/B1-207[»]
2F3TX-ray3.16A/B/C/D/E/F1-207[»]
ProteinModelPortaliP60546
SMRiP60546
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262568, 25 interactors
IntActiP60546, 5 interactors
STRINGi316385.ECDH10B_3830

Chemistry databases

ChEMBLiCHEMBL3309016

Proteomic databases

EPDiP60546
PaxDbiP60546
PRIDEiP60546

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76672; AAC76672; b3648
BAE77645; BAE77645; BAE77645
GeneIDi948163
KEGGiecj:JW3623
eco:b3648
PATRICifig|511145.12.peg.3768

Organism-specific databases

EchoBASEiEB0958
EcoGeneiEG10965 gmk

Phylogenomic databases

eggNOGiENOG4108UHA Bacteria
COG0194 LUCA
HOGENOMiHOG000037638
InParanoidiP60546
KOiK00942
PhylomeDBiP60546

Enzyme and pathway databases

BioCyciEcoCyc:GUANYL-KIN-MONOMER
MetaCyc:GUANYL-KIN-MONOMER
BRENDAi2.7.4.8 2026

Miscellaneous databases

EvolutionaryTraceiP60546
PROiPR:P60546

Family and domain databases

HAMAPiMF_00328 Guanylate_kinase, 1 hit
InterProiView protein in InterPro
IPR008145 GK/Ca_channel_bsu
IPR008144 Guanylate_kin-like_dom
IPR017665 Guanylate_kinase
IPR020590 Guanylate_kinase_CS
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00625 Guanylate_kin, 1 hit
SMARTiView protein in SMART
SM00072 GuKc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR03263 guanyl_kin, 1 hit
PROSITEiView protein in PROSITE
PS00856 GUANYLATE_KINASE_1, 1 hit
PS50052 GUANYLATE_KINASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKGUA_ECOLI
AccessioniPrimary (citable) accession number: P60546
Secondary accession number(s): P24234, Q2M7W1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 15, 2004
Last modified: November 7, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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