UniProtKB - P60546 (KGUA_ECOLI)

BLAST

>sp|P60546|KGUA_ECOLI Guanylate kinase OS=Escherichia coli (strain K12) OX=83333 GN=gmk PE=1 SV=1
MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQPRPGEVHGEHYFFVNHD
EFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLDIDWQGAQQIRQKMPHARS
IFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHYAEYDYLIVNDDFDTALTDLK
TIIRAERLRMSRQKQRHDALISKLLAD

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History
Entry version 121 (07 Nov 2018)
Sequence version 1 (15 Mar 2004)
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Protein

Guanylate kinase

Gene

gmk

Organism

Escherichia coli (strain K12)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Essential for recycling GMP and indirectly, cGMP.1 Publication

Catalytic activityⁱ

ATP + GMP = ADP + GDP.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	11 – 18	ATPBy similarity		8

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-UniRule
guanylate kinase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 8390989
identical protein binding
Source: EcoCyc
Inferred from direct assayⁱ
- 16140325
- 8390989

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Transferase
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:GUANYL-KIN-MONOMER MetaCyc:GUANYL-KIN-MONOMER
BRENDAⁱ	2.7.4.8 2026

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Guanylate kinase (EC:2.7.4.8) Alternative name(s): GMP kinase
Gene namesⁱ	Name:gmk Synonyms:spoR Ordered Locus Names:b3648, JW3623
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10965 gmk

EcoGeneⁱ

EG10965 gmk

Subcellular locationⁱ

Cytoplasm

GO - Cellular componentⁱ

cytosol
Source: EcoCyc
Inferred from direct assayⁱ
- 18304323

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Chemistry databases

ChEMBLⁱ	CHEMBL3309016

ChEMBLⁱ

CHEMBL3309016

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000170534	1 – 207	Guanylate kinaseAdd BLAST		207

Proteomic databases

EPDⁱ	P60546
PaxDbⁱ	P60546
PRIDEⁱ	P60546

Interactionⁱ

Subunit structureⁱ

Homotetramer (under low ionic conditions) or homodimer (under high ionic conditions).1 Publication

GO - Molecular functionⁱ

identical protein binding
Source: EcoCyc
Inferred from direct assayⁱ
- 16140325
- 8390989

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	4262568, 25 interactors
IntActⁱ	P60546, 5 interactors
STRINGⁱ	316385.ECDH10B_3830

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	6 – 10	Combined sources	5
Beta strandⁱ	13 – 15	Combined sources	3
Helixⁱ	17 – 27	Combined sources	11
Turnⁱ	30 – 32	Combined sources	3
Beta strandⁱ	33 – 35	Combined sources	3
Beta strandⁱ	38 – 42	Combined sources	5
Turnⁱ	50 – 52	Combined sources	3
Beta strandⁱ	53 – 56	Combined sources	4
Helixⁱ	59 – 67	Combined sources	9
Beta strandⁱ	71 – 77	Combined sources	7
Beta strandⁱ	80 – 85	Combined sources	6
Helixⁱ	86 – 93	Combined sources	8
Turnⁱ	94 – 96	Combined sources	3
Beta strandⁱ	98 – 102	Combined sources	5
Helixⁱ	105 – 114	Combined sources	10
Beta strandⁱ	119 – 124	Combined sources	6
Helixⁱ	128 – 136	Combined sources	9
Beta strandⁱ	138 – 141	Combined sources	4
Helixⁱ	143 – 157	Combined sources	15
Helixⁱ	158 – 162	Combined sources	5
Beta strandⁱ	163 – 168	Combined sources	6
Helixⁱ	172 – 187	Combined sources	16
Helixⁱ	190 – 196	Combined sources	7
Helixⁱ	198 – 205	Combined sources	8

Show more details

3D structure databases

ProteinModelPortalⁱ	P60546
SMRⁱ	P60546
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P60546

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	4 – 184	Guanylate kinase-likeAdd BLAST		181

Sequence similaritiesⁱ

Belongs to the guanylate kinase family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4108UHA Bacteria COG0194 LUCA
HOGENOMⁱ	HOG000037638
InParanoidⁱ	P60546
KEGG Orthology (KO) More...KOⁱ	K00942
PhylomeDBⁱ	P60546

Family and domain databases

HAMAPⁱ	MF_00328 Guanylate_kinase, 1 hit
InterProⁱ	View protein in InterPro IPR008145 GK/Ca_channel_bsu IPR008144 Guanylate_kin-like_dom IPR017665 Guanylate_kinase IPR020590 Guanylate_kinase_CS IPR027417 P-loop_NTPase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00625 Guanylate_kin, 1 hit
SMARTⁱ	View protein in SMART SM00072 GuKc, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit
TIGRFAMsⁱ	TIGR03263 guanyl_kin, 1 hit
PROSITEⁱ	View protein in PROSITE PS00856 GUANYLATE_KINASE_1, 1 hit PS50052 GUANYLATE_KINASE_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P60546-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAQGTLYIVS APSGAGKSSL IQALLKTQPL YDTQVSVSHT TRQPRPGEVH 
        60         70         80         90        100
GEHYFFVNHD EFKEMISRDA FLEHAEVFGN YYGTSREAIE QVLATGVDVF 
       110        120        130        140        150
LDIDWQGAQQ IRQKMPHARS IFILPPSKIE LDRRLRGRGQ DSEEVIAKRM 
       160        170        180        190        200
AQAVAEMSHY AEYDYLIVND DFDTALTDLK TIIRAERLRM SRQKQRHDAL 

ISKLLAD

Length:207

Mass (Da):23,593

Last modified:March 15, 2004 - v1

Checksum:ⁱ62A99DB4063651E4

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M84400 Genomic DNA Translation: AAB88711.1 L10328 Genomic DNA Translation: AAA62001.1 U00096 Genomic DNA Translation: AAC76672.1 AP009048 Genomic DNA Translation: BAE77645.1
PIRⁱ	S43041 KIECGU
NCBI Reference Sequences More...RefSeqⁱ	NP_418105.1, NC_000913.3 WP_001295237.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC76672; AAC76672; b3648 BAE77645; BAE77645; BAE77645
GeneIDⁱ	948163
KEGGⁱ	ecj:JW3623 eco:b3648
PATRICⁱ	fig\|511145.12.peg.3768

Protein	Similar proteins		Species	Score	Length	Source
P60546	Guanylate kinase		ECOL5		207	UniRef100_Q0TBG1
Guanylate kinase		ECOL6		207
Guanylate kinase		ECOUT		207
Guanylate kinase		SHIFL		207
Guanylate kinase		SHISS		207
+166

Protein	Similar proteins		Species	Score	Length	Source
P60546	Guanylate kinase		ECOL5		207	UniRef90_Q0TBG1
Guanylate kinase		ECOL6		207
Guanylate kinase		ECOUT		207
Guanylate kinase		SHIFL		207
Guanylate kinase		SHISS		207
+214

Protein	Similar proteins		Species	Score	Length	Source
P60546	Guanylate kinase		VIBCH		207	UniRef50_Q9KNM4
Guanylate kinase		ECOL5		207
Guanylate kinase		ECOL6		207
Guanylate kinase		ECOUT		207
Guanylate kinase		SHIFL		207
+257

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M84400 Genomic DNA Translation: AAB88711.1 L10328 Genomic DNA Translation: AAA62001.1 U00096 Genomic DNA Translation: AAC76672.1 AP009048 Genomic DNA Translation: BAE77645.1
PIRⁱ	S43041 KIECGU
RefSeqⁱ	NP_418105.1, NC_000913.3 WP_001295237.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1S96	X-ray	2.00	A/B	1-207	[»]
	2AN9	X-ray	2.35	A/B	1-207	[»]
	2ANB	X-ray	2.90	A	1-207	[»]
	2ANC	X-ray	3.20	A/B/C/D/E/F	1-207	[»]
	2F3R	X-ray	2.50	A/B	1-207	[»]
	2F3T	X-ray	3.16	A/B/C/D/E/F	1-207	[»]
ProteinModelPortalⁱ	P60546
SMRⁱ	P60546
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4262568, 25 interactors
IntActⁱ	P60546, 5 interactors
STRINGⁱ	316385.ECDH10B_3830

Chemistry databases

ChEMBLⁱ	CHEMBL3309016

ChEMBLⁱ

CHEMBL3309016

Proteomic databases

EPDⁱ	P60546
PaxDbⁱ	P60546
PRIDEⁱ	P60546

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC76672; AAC76672; b3648 BAE77645; BAE77645; BAE77645
GeneIDⁱ	948163
KEGGⁱ	ecj:JW3623 eco:b3648
PATRICⁱ	fig\|511145.12.peg.3768

Organism-specific databases

EchoBASEⁱ	EB0958
EcoGeneⁱ	EG10965 gmk

Phylogenomic databases

eggNOGⁱ	ENOG4108UHA Bacteria COG0194 LUCA
HOGENOMⁱ	HOG000037638
InParanoidⁱ	P60546
KOⁱ	K00942
PhylomeDBⁱ	P60546

Enzyme and pathway databases

BioCycⁱ	EcoCyc:GUANYL-KIN-MONOMER MetaCyc:GUANYL-KIN-MONOMER
BRENDAⁱ	2.7.4.8 2026

Miscellaneous databases

EvolutionaryTraceⁱ	P60546
Protein Ontology More...PROⁱ	PR:P60546

Family and domain databases

HAMAPⁱ	MF_00328 Guanylate_kinase, 1 hit
InterProⁱ	View protein in InterPro IPR008145 GK/Ca_channel_bsu IPR008144 Guanylate_kin-like_dom IPR017665 Guanylate_kinase IPR020590 Guanylate_kinase_CS IPR027417 P-loop_NTPase
Pfamⁱ	View protein in Pfam PF00625 Guanylate_kin, 1 hit
SMARTⁱ	View protein in SMART SM00072 GuKc, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit
TIGRFAMsⁱ	TIGR03263 guanyl_kin, 1 hit
PROSITEⁱ	View protein in PROSITE PS00856 GUANYLATE_KINASE_1, 1 hit PS50052 GUANYLATE_KINASE_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	KGUA_ECOLI
Accessionⁱ	P60546Primary (citable) accession number: P60546 Secondary accession number(s): P24234, Q2M7W1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
	Last sequence update:	March 15, 2004
	Last modified:	November 7, 2018
	This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

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UniProtKB - P60546 (KGUA_ECOLI)

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Guanylate kinase

gmk

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ