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Protein

Gamma-aminobutyric acid receptor-associated protein-like 2

Gene

Gabarapl2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (By similarity).By similarity

GO - Biological processi

  • autophagosome assembly Source: GO_Central
  • autophagy of mitochondrion Source: GO_Central
  • cellular response to nitrogen starvation Source: GO_Central
  • protein transport Source: UniProtKB-KW

Keywordsi

Biological processAutophagy, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-1632852 Macroautophagy
R-RNO-8854214 TBC/RABGAPs

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor-associated protein-like 2
Alternative name(s):
GABA(A) receptor-associated protein-like 2
Ganglioside expression factor 2
Short name:
GEF-2
Golgi-associated ATPase enhancer of 16 kDa
Short name:
GATE-16
Gene namesi
Name:Gabarapl2
Synonyms:Gef2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi620510 Gabarapl2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002123751 – 116Gamma-aminobutyric acid receptor-associated protein-like 2Add BLAST116
PropeptideiPRO_0000423072117Removed in mature formBy similarity1

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24N6-acetyllysineBy similarity1
Modified residuei39PhosphoserineBy similarity1
Lipidationi116Phosphatidylethanolamine amidated glycineBy similarity1

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL2-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL2-II. ATG4B also mediates the delipidation required for GABARAPL1 recycling when autophagosomes fuse with lysosomes (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei116 – 117Cleavage; by ATG4BBy similarity2

Keywords - PTMi

Acetylation, Lipoprotein, Phosphoprotein

Proteomic databases

PaxDbiP60522
PRIDEiP60522

PTM databases

PhosphoSitePlusiP60522

Expressioni

Gene expression databases

BgeeiENSRNOG00000019425 Expressed in 9 organ(s), highest expression level in spleen
GenevisibleiP60522 RN

Interactioni

Subunit structurei

Monomer. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin. Interacts with ATG3, ATG7, ATG13 and ULK1. Interacts with TP53INP1 and TP53INP2. Interacts with TBC1D25. Directly interacts with SQSTM1 and BNIP3. Interacts with TECPR2 and PCM1. Interacts with TBC1D5. Interacts with TBC1D5. Interacts with TRIM5. Interacts with MEFV and TRIM21. Interacts with WDFY3.By similarity

Protein-protein interaction databases

CORUMiP60522
STRINGi10116.ENSRNOP00000066243

Structurei

3D structure databases

ProteinModelPortaliP60522
SMRiP60522
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 22Interaction with beta-tubulinBy similarityAdd BLAST22
Regioni36 – 68Interaction with GABRG2Sequence analysisAdd BLAST33

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiKOG1654 Eukaryota
ENOG4111JAT LUCA
GeneTreeiENSGT00390000012937
HOGENOMiHOG000232034
HOVERGENiHBG051706
InParanoidiP60522
KOiK08341
OMAiAAAMKWM
OrthoDBiEOG091G10QX
PhylomeDBiP60522
TreeFamiTF312964

Family and domain databases

CDDicd01611 GABARAP, 1 hit
InterProiView protein in InterPro
IPR004241 Atg8-like
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR10969 PTHR10969, 1 hit
PfamiView protein in Pfam
PF02991 Atg8, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60522-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKWMFKEDHS LEHRCVESAK IRAKYPDRVP VIVEKVSGSQ IVDIDKRKYL
60 70 80 90 100
VPSDITVAQF MWIIRKRIQL PSEKAIFLFV DKTVPQSSLT MGQLYEKEKD
110
EDGFLYVAYS GENTFGF
Length:117
Mass (Da):13,667
Last modified:March 1, 2004 - v1
Checksum:i17ACB540FD5B975B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003515 mRNA Translation: BAA19975.1
BC058145 mRNA Translation: AAH58145.1
BC088139 mRNA Translation: AAH88139.1
RefSeqiNP_073197.1, NM_022706.2
XP_002729993.3, XM_002729947.5
UniGeneiRn.120952
Rn.64537

Genome annotation databases

EnsembliENSRNOT00000048998; ENSRNOP00000040067; ENSRNOG00000019425
GeneIDi100359937
64670
KEGGirno:100359937
rno:64670
UCSCiRGD:620510 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003515 mRNA Translation: BAA19975.1
BC058145 mRNA Translation: AAH58145.1
BC088139 mRNA Translation: AAH88139.1
RefSeqiNP_073197.1, NM_022706.2
XP_002729993.3, XM_002729947.5
UniGeneiRn.120952
Rn.64537

3D structure databases

ProteinModelPortaliP60522
SMRiP60522
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

CORUMiP60522
STRINGi10116.ENSRNOP00000066243

PTM databases

PhosphoSitePlusiP60522

Proteomic databases

PaxDbiP60522
PRIDEiP60522

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000048998; ENSRNOP00000040067; ENSRNOG00000019425
GeneIDi100359937
64670
KEGGirno:100359937
rno:64670
UCSCiRGD:620510 rat

Organism-specific databases

CTDi11345
RGDi620510 Gabarapl2

Phylogenomic databases

eggNOGiKOG1654 Eukaryota
ENOG4111JAT LUCA
GeneTreeiENSGT00390000012937
HOGENOMiHOG000232034
HOVERGENiHBG051706
InParanoidiP60522
KOiK08341
OMAiAAAMKWM
OrthoDBiEOG091G10QX
PhylomeDBiP60522
TreeFamiTF312964

Enzyme and pathway databases

ReactomeiR-RNO-1632852 Macroautophagy
R-RNO-8854214 TBC/RABGAPs

Miscellaneous databases

PROiPR:P60522

Gene expression databases

BgeeiENSRNOG00000019425 Expressed in 9 organ(s), highest expression level in spleen
GenevisibleiP60522 RN

Family and domain databases

CDDicd01611 GABARAP, 1 hit
InterProiView protein in InterPro
IPR004241 Atg8-like
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR10969 PTHR10969, 1 hit
PfamiView protein in Pfam
PF02991 Atg8, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGBRL2_RAT
AccessioniPrimary (citable) accession number: P60522
Secondary accession number(s): O08765, Q9DCP8, Q9UQF7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: November 7, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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