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Protein

Gamma-aminobutyric acid receptor-associated protein-like 2

Gene

Gabarapl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity). Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAutophagy, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-1632852 Macroautophagy
R-MMU-8854214 TBC/RABGAPs

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor-associated protein-like 2
Alternative name(s):
GABA(A) receptor-associated protein-like 2
Golgi-associated ATPase enhancer of 16 kDa
Short name:
GATE-16
Gene namesi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1890602 Gabarapl2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002123741 – 116Gamma-aminobutyric acid receptor-associated protein-like 2Add BLAST116
PropeptideiPRO_0000423071117Removed in mature form1

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24N6-acetyllysineBy similarity1
Modified residuei39PhosphoserineBy similarity1
Lipidationi116Phosphatidylethanolamine amidated glycineBy similarity1

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL2-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL2-II. ATG4B also mediates the delipidation required for GABARAPL1 recycling when autophagosomes fuse with lysosomes.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei116 – 117Cleavage; by ATG4B2

Keywords - PTMi

Acetylation, Lipoprotein, Phosphoprotein

Proteomic databases

EPDiP60521
MaxQBiP60521
PaxDbiP60521
PeptideAtlasiP60521
PRIDEiP60521

PTM databases

iPTMnetiP60521
PhosphoSitePlusiP60521
SwissPalmiP60521

Expressioni

Tissue specificityi

Ubiquitous. A high level expression is seen in brain, thymus, lung, heart, liver and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000031950 Expressed in 20 organ(s), highest expression level in cerebellum
GenevisibleiP60521 MM

Interactioni

Subunit structurei

Monomer. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin. Interacts with ATG3, ATG13 and ULK1. Interacts with TP53INP1 and TP53INP2. Interacts with TBC1D25 and ATG7. Directly interacts with SQSTM1 and BNIP3. Interacts with TECPR2 and PCM1. Interacts with TBC1D5. Interacts with TRIM5. Interacts with MEFV and TRIM21. Interacts with WDFY3.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi220282, 1 interactor
ELMiP60521
STRINGi10090.ENSMUSP00000034428

Structurei

3D structure databases

ProteinModelPortaliP60521
SMRiP60521
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 22Interaction with beta-tubulinBy similarityAdd BLAST22
Regioni36 – 68Interaction with GABRG2Sequence analysisAdd BLAST33

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiKOG1654 Eukaryota
ENOG4111JAT LUCA
GeneTreeiENSGT00390000012937
HOGENOMiHOG000232034
HOVERGENiHBG051706
InParanoidiP60521
KOiK08341
OMAiAAAMKWM
OrthoDBiEOG091G10QX
PhylomeDBiP60521
TreeFamiTF312964

Family and domain databases

CDDicd01611 GABARAP, 1 hit
InterProiView protein in InterPro
IPR004241 Atg8-like
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR10969 PTHR10969, 1 hit
PfamiView protein in Pfam
PF02991 Atg8, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60521-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKWMFKEDHS LEHRCVESAK IRAKYPDRVP VIVEKVSGSQ IVDIDKRKYL
60 70 80 90 100
VPSDITVAQF MWIIRKRIQL PSEKAIFLFV DKTVPQSSLT MGQLYEKEKD
110
EDGFLYVAYS GENTFGF
Length:117
Mass (Da):13,667
Last modified:March 1, 2004 - v1
Checksum:i17ACB540FD5B975B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83T → A in BAB22217 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF190644 mRNA Translation: AAK16238.1
AK002596 mRNA Translation: BAB22217.1
AK049819 mRNA Translation: BAC33933.1
BC026798 mRNA Translation: AAH26798.1
BC081436 mRNA Translation: AAH81436.1
CCDSiCCDS40480.1
RefSeqiNP_080969.2, NM_026693.5
UniGeneiMm.371666

Genome annotation databases

EnsembliENSMUST00000034428; ENSMUSP00000034428; ENSMUSG00000031950
GeneIDi93739
KEGGimmu:93739
UCSCiuc009nnb.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF190644 mRNA Translation: AAK16238.1
AK002596 mRNA Translation: BAB22217.1
AK049819 mRNA Translation: BAC33933.1
BC026798 mRNA Translation: AAH26798.1
BC081436 mRNA Translation: AAH81436.1
CCDSiCCDS40480.1
RefSeqiNP_080969.2, NM_026693.5
UniGeneiMm.371666

3D structure databases

ProteinModelPortaliP60521
SMRiP60521
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi220282, 1 interactor
ELMiP60521
STRINGi10090.ENSMUSP00000034428

PTM databases

iPTMnetiP60521
PhosphoSitePlusiP60521
SwissPalmiP60521

Proteomic databases

EPDiP60521
MaxQBiP60521
PaxDbiP60521
PeptideAtlasiP60521
PRIDEiP60521

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034428; ENSMUSP00000034428; ENSMUSG00000031950
GeneIDi93739
KEGGimmu:93739
UCSCiuc009nnb.2 mouse

Organism-specific databases

CTDi11345
MGIiMGI:1890602 Gabarapl2

Phylogenomic databases

eggNOGiKOG1654 Eukaryota
ENOG4111JAT LUCA
GeneTreeiENSGT00390000012937
HOGENOMiHOG000232034
HOVERGENiHBG051706
InParanoidiP60521
KOiK08341
OMAiAAAMKWM
OrthoDBiEOG091G10QX
PhylomeDBiP60521
TreeFamiTF312964

Enzyme and pathway databases

ReactomeiR-MMU-1632852 Macroautophagy
R-MMU-8854214 TBC/RABGAPs

Miscellaneous databases

ChiTaRSiGabarapl2 mouse
PROiPR:P60521
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031950 Expressed in 20 organ(s), highest expression level in cerebellum
GenevisibleiP60521 MM

Family and domain databases

CDDicd01611 GABARAP, 1 hit
InterProiView protein in InterPro
IPR004241 Atg8-like
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR10969 PTHR10969, 1 hit
PfamiView protein in Pfam
PF02991 Atg8, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGBRL2_MOUSE
AccessioniPrimary (citable) accession number: P60521
Secondary accession number(s): O08765, Q9DCP8, Q9UQF7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: September 12, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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