UniProtKB - P60520 (GBRL2_HUMAN)
Gamma-aminobutyric acid receptor-associated protein-like 2
GABARAPL2
Functioni
GO - Molecular functioni
- ATPase binding Source: UniProtKB
- beta-tubulin binding Source: UniProtKB
- GABA receptor binding Source: UniProtKB
- microtubule binding Source: UniProtKB
- SNARE binding Source: UniProtKB
- ubiquitin protein ligase binding Source: UniProtKB
GO - Biological processi
- autophagosome assembly Source: GO_Central
- autophagosome maturation Source: Reactome
- autophagy Source: UniProtKB
- autophagy of mitochondrion Source: GO_Central
- cellular response to nitrogen starvation Source: GO_Central
- intra-Golgi vesicle-mediated transport Source: UniProtKB
- macroautophagy Source: GO_Central
- negative regulation of proteasomal protein catabolic process Source: BHF-UCL
- positive regulation of ATPase activity Source: UniProtKB
- protein localization to endoplasmic reticulum Source: UniProtKB
- protein transport Source: UniProtKB-KW
Keywordsi
Biological process | Autophagy, Protein transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | P60520 |
Reactomei | R-HSA-1632852, Macroautophagy R-HSA-8854214, TBC/RABGAPs |
SIGNORi | P60520 |
Protein family/group databases
MoonDBi | P60520, Predicted |
Names & Taxonomyi
Protein namesi | Recommended name: Gamma-aminobutyric acid receptor-associated protein-like 2Alternative name(s): GABA(A) receptor-associated protein-like 2 Ganglioside expression factor 2 Short name: GEF-2 General protein transport factor p16 Golgi-associated ATPase enhancer of 16 kDa Short name: GATE-16 MAP1 light chain 3-related protein |
Gene namesi | Name:GABARAPL2 Synonyms:FLC3A, GEF2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:13291, GABARAPL2 |
MIMi | 607452, gene |
neXtProti | NX_P60520 |
VEuPathDBi | HostDB:ENSG00000034713.7 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus By similarity
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication
Other locations
- autophagosome 5 Publications
Cytosol
- cytosol Source: UniProtKB
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi membrane Source: BHF-UCL
Other locations
- autophagosome Source: UniProtKB
- autophagosome membrane Source: UniProtKB
- cytoplasm Source: BHF-UCL
- cytoplasmic vesicle Source: UniProtKB-KW
Keywords - Cellular componenti
Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 47 | R → A: Strongly reduced interaction with UBA5. 1 Publication | 1 | |
Mutagenesisi | 116 | G → A: Impairs localization at the autophagosomal membrane. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 11345 |
OpenTargetsi | ENSG00000034713 |
PharmGKBi | PA28482 |
Miscellaneous databases
Pharosi | P60520, Tbio |
Genetic variation databases
BioMutai | GABARAPL2 |
DMDMi | 44888808 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000212373 | 1 – 116 | Gamma-aminobutyric acid receptor-associated protein-like 2Add BLAST | 116 | |
PropeptideiPRO_0000423070 | 117 | Removed in mature form1 Publication | 1 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 24 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 39 | PhosphoserineCombined sources | 1 | |
Lipidationi | 116 | Phosphatidylethanolamine amidated glycine1 Publication | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 116 – 117 | Cleavage; by ATG41 Publication | 2 |
Keywords - PTMi
Acetylation, Lipoprotein, PhosphoproteinProteomic databases
EPDi | P60520 |
jPOSTi | P60520 |
MassIVEi | P60520 |
PaxDbi | P60520 |
PeptideAtlasi | P60520 |
PRIDEi | P60520 |
ProteomicsDBi | 57214 |
2D gel databases
UCD-2DPAGEi | P60520 |
PTM databases
iPTMneti | P60520 |
PhosphoSitePlusi | P60520 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000034713, Expressed in caudate nucleus and 249 other tissues |
ExpressionAtlasi | P60520, baseline and differential |
Genevisiblei | P60520, HS |
Organism-specific databases
HPAi | ENSG00000034713, Low tissue specificity |
Interactioni
Subunit structurei
Monomer.
Interacts with ATG3, ATG7, ATG13 and ULK1 (PubMed:11146101, PubMed:11825910, PubMed:11096062, PubMed:12507496, PubMed:23043107).
Interacts with TP53INP1 and TP53INP2 (PubMed:19056683, PubMed:22421968, PubMed:22470510).
Interacts with TBC1D25 (PubMed:21383079). Directly interacts with SQSTM1 and BNIP3 (PubMed:17580304, PubMed:23209295).
Interacts with TECPR2 and PCM1 (PubMed:20562859, PubMed:24089205).
Interacts with TBC1D5 (PubMed:22354992).
Interacts with TRIM5 (PubMed:25127057).
Interacts with MEFV and TRIM21 (PubMed:26347139).
Interacts with WDFY3 (PubMed:24668264).
Interacts with UBA5; promoting recruitment of UBA5 to the endoplasmic reticulum membrane (PubMed:26929408, PubMed:30990354).
Interacts with GOSR1 (By similarity).
By similarity19 PublicationsBinary interactionsi
P60520
GO - Molecular functioni
- ATPase binding Source: UniProtKB
- beta-tubulin binding Source: UniProtKB
- GABA receptor binding Source: UniProtKB
- microtubule binding Source: UniProtKB
- SNARE binding Source: UniProtKB
- ubiquitin protein ligase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 116473, 130 interactors |
DIPi | DIP-35051N |
ELMi | P60520 |
IntActi | P60520, 535 interactors |
MINTi | P60520 |
STRINGi | 9606.ENSP00000037243 |
Miscellaneous databases
RNActi | P60520, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P60520 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1654, Eukaryota |
GeneTreei | ENSGT00940000155010 |
HOGENOMi | CLU_119276_0_1_1 |
InParanoidi | P60520 |
OMAi | AKMKWMF |
OrthoDBi | 1508198at2759 |
PhylomeDBi | P60520 |
TreeFami | TF312964 |
Family and domain databases
InterProi | View protein in InterPro IPR004241, Atg8-like IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR10969, PTHR10969, 1 hit |
Pfami | View protein in Pfam PF02991, Atg8, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MKWMFKEDHS LEHRCVESAK IRAKYPDRVP VIVEKVSGSQ IVDIDKRKYL
60 70 80 90 100
VPSDITVAQF MWIIRKRIQL PSEKAIFLFV DKTVPQSSLT MGQLYEKEKD
110
EDGFLYVAYS GENTFGF
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BQ50 | H3BQ50_HUMAN | Gamma-aminobutyric acid receptor-as... | GABARAPL2 | 57 | Annotation score: | ||
H3BSM5 | H3BSM5_HUMAN | Gamma-aminobutyric acid receptor-as... | GABARAPL2 | 88 | Annotation score: | ||
H3BU36 | H3BU36_HUMAN | GABA(A) receptor-associated protein... | GABARAPL2 hCG_21766 | 42 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 29 | V → F in AAD27779 (Ref. 4) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_049756 | 51 | V → A. Corresponds to variant dbSNP:rs11556291Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB030710 mRNA Translation: BAB21548.1 AF087848 mRNA Translation: AAK20400.1 AJ010569 mRNA Translation: CAA09249.1 AF077046 mRNA Translation: AAD27779.1 CR542217 mRNA Translation: CAG47013.1 AK289788 mRNA Translation: BAF82477.1 CH471114 Genomic DNA Translation: EAW95630.1 BC005985 mRNA Translation: AAH05985.1 BC014594 mRNA Translation: AAH14594.1 BC029601 mRNA Translation: AAH29601.1 |
CCDSi | CCDS10921.1 |
RefSeqi | NP_009216.1, NM_007285.6 |
Genome annotation databases
Ensembli | ENST00000037243; ENSP00000037243; ENSG00000034713 |
GeneIDi | 11345 |
KEGGi | hsa:11345 |
UCSCi | uc002fen.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB030710 mRNA Translation: BAB21548.1 AF087848 mRNA Translation: AAK20400.1 AJ010569 mRNA Translation: CAA09249.1 AF077046 mRNA Translation: AAD27779.1 CR542217 mRNA Translation: CAG47013.1 AK289788 mRNA Translation: BAF82477.1 CH471114 Genomic DNA Translation: EAW95630.1 BC005985 mRNA Translation: AAH05985.1 BC014594 mRNA Translation: AAH14594.1 BC029601 mRNA Translation: AAH29601.1 |
CCDSi | CCDS10921.1 |
RefSeqi | NP_009216.1, NM_007285.6 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4CO7 | X-ray | 2.00 | A/B | 1-117 | [»] | |
6H8C | NMR | - | A | 1-116 | [»] | |
SMRi | P60520 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116473, 130 interactors |
DIPi | DIP-35051N |
ELMi | P60520 |
IntActi | P60520, 535 interactors |
MINTi | P60520 |
STRINGi | 9606.ENSP00000037243 |
Protein family/group databases
MoonDBi | P60520, Predicted |
PTM databases
iPTMneti | P60520 |
PhosphoSitePlusi | P60520 |
Genetic variation databases
BioMutai | GABARAPL2 |
DMDMi | 44888808 |
2D gel databases
UCD-2DPAGEi | P60520 |
Proteomic databases
EPDi | P60520 |
jPOSTi | P60520 |
MassIVEi | P60520 |
PaxDbi | P60520 |
PeptideAtlasi | P60520 |
PRIDEi | P60520 |
ProteomicsDBi | 57214 |
Protocols and materials databases
Antibodypediai | 30328, 550 antibodies |
DNASUi | 11345 |
Genome annotation databases
Ensembli | ENST00000037243; ENSP00000037243; ENSG00000034713 |
GeneIDi | 11345 |
KEGGi | hsa:11345 |
UCSCi | uc002fen.3, human |
Organism-specific databases
CTDi | 11345 |
DisGeNETi | 11345 |
GeneCardsi | GABARAPL2 |
HGNCi | HGNC:13291, GABARAPL2 |
HPAi | ENSG00000034713, Low tissue specificity |
MIMi | 607452, gene |
neXtProti | NX_P60520 |
OpenTargetsi | ENSG00000034713 |
PharmGKBi | PA28482 |
VEuPathDBi | HostDB:ENSG00000034713.7 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1654, Eukaryota |
GeneTreei | ENSGT00940000155010 |
HOGENOMi | CLU_119276_0_1_1 |
InParanoidi | P60520 |
OMAi | AKMKWMF |
OrthoDBi | 1508198at2759 |
PhylomeDBi | P60520 |
TreeFami | TF312964 |
Enzyme and pathway databases
PathwayCommonsi | P60520 |
Reactomei | R-HSA-1632852, Macroautophagy R-HSA-8854214, TBC/RABGAPs |
SIGNORi | P60520 |
Miscellaneous databases
BioGRID-ORCSi | 11345, 4 hits in 882 CRISPR screens |
ChiTaRSi | GABARAPL2, human |
GeneWikii | GABARAPL2 |
GenomeRNAii | 11345 |
Pharosi | P60520, Tbio |
PROi | PR:P60520 |
RNActi | P60520, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000034713, Expressed in caudate nucleus and 249 other tissues |
ExpressionAtlasi | P60520, baseline and differential |
Genevisiblei | P60520, HS |
Family and domain databases
InterProi | View protein in InterPro IPR004241, Atg8-like IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR10969, PTHR10969, 1 hit |
Pfami | View protein in Pfam PF02991, Atg8, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GBRL2_HUMAN | |
Accessioni | P60520Primary (citable) accession number: P60520 Secondary accession number(s): O08765 Q9UQF7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2004 |
Last sequence update: | March 1, 2004 | |
Last modified: | February 10, 2021 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families