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Protein

Gamma-aminobutyric acid receptor-associated protein-like 2

Gene

GABARAPL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity). Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.By similarity2 Publications

GO - Molecular functioni

  • ATPase binding Source: UniProtKB
  • beta-tubulin binding Source: UniProtKB
  • GABA receptor binding Source: UniProtKB
  • microtubule binding Source: UniProtKB
  • SNARE binding Source: UniProtKB
  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processAutophagy, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-1632852 Macroautophagy
R-HSA-8854214 TBC/RABGAPs
SIGNORiP60520

Protein family/group databases

MoonDBiP60520 Predicted

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor-associated protein-like 2
Alternative name(s):
GABA(A) receptor-associated protein-like 2
Ganglioside expression factor 2
Short name:
GEF-2
General protein transport factor p16
Golgi-associated ATPase enhancer of 16 kDa
Short name:
GATE-16
MAP1 light chain 3-related protein
Gene namesi
Name:GABARAPL2
Synonyms:FLC3A, GEF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000034713.7
HGNCiHGNC:13291 GABARAPL2
MIMi607452 gene
neXtProtiNX_P60520

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi116G → A: Impairs localization at the autophagosomal membrane. 1 Publication1

Organism-specific databases

DisGeNETi11345
OpenTargetsiENSG00000034713
PharmGKBiPA28482

Polymorphism and mutation databases

BioMutaiGABARAPL2
DMDMi44888808

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002123731 – 116Gamma-aminobutyric acid receptor-associated protein-like 2Add BLAST116
PropeptideiPRO_0000423070117Removed in mature form1

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24N6-acetyllysineCombined sources1
Modified residuei39PhosphoserineCombined sources1
Lipidationi116Phosphatidylethanolamine amidated glycine1 Publication1

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL2-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL2-II. ATG4B also mediates the delipidation required for GABARAPL1 recycling when autophagosomes fuse with lysosomes.1 Publication
The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei116 – 117Cleavage; by ATG42

Keywords - PTMi

Acetylation, Lipoprotein, Phosphoprotein

Proteomic databases

EPDiP60520
PaxDbiP60520
PeptideAtlasiP60520
PRIDEiP60520
ProteomicsDBi57214

2D gel databases

UCD-2DPAGEiP60520

PTM databases

iPTMnetiP60520
PhosphoSitePlusiP60520

Miscellaneous databases

PMAP-CutDBiP60520

Expressioni

Tissue specificityi

Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine.2 Publications

Gene expression databases

BgeeiENSG00000034713 Expressed in 238 organ(s), highest expression level in caudate nucleus
CleanExiHS_GABARAPL2
ExpressionAtlasiP60520 baseline and differential
GenevisibleiP60520 HS

Organism-specific databases

HPAiHPA036726

Interactioni

Subunit structurei

Monomer. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin (By similarity). Interacts with ATG3, ATG7, ATG13 and ULK1. Interacts with TP53INP1 and TP53INP2. Interacts with TBC1D25. Directly interacts with SQSTM1 and BNIP3. Interacts with TECPR2 and PCM1. Interacts with TBC1D5 (PubMed:22354992). Interacts with TRIM5 (PubMed:25127057). Interacts with MEFV and TRIM21 (PubMed:26347139). Interacts with WDFY3 (PubMed:24668264).By similarity17 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AGTRAPQ6RW133EBI-720116,EBI-741181
AHNAK2Q8IVF2-23EBI-720116,EBI-10217765
ANKFY1Q9P2R32EBI-720116,EBI-2513908
ARFGAP1Q8N6T33EBI-720116,EBI-716933
ATG13O751433EBI-720116,EBI-2798775
ATG16L1Q676U52EBI-720116,EBI-535909
ATG2AQ2TAZ02EBI-720116,EBI-2514077
ATG3Q9NT625EBI-720116,EBI-988094
ATG4BQ9Y4P19EBI-720116,EBI-712014
ATG7O953527EBI-720116,EBI-987834
BCL2L13Q9BXK54EBI-720116,EBI-747430
Bnip3lQ9Z2F72EBI-720116,EBI-1774669From Mus musculus.
CALCOCO1Q9P1Z23EBI-720116,EBI-749920
CALCOCO2Q131376EBI-720116,EBI-739580
CCDC155Q8N6L03EBI-720116,EBI-749265
DNAAF4Q8WXU22EBI-720116,EBI-2946907
EGFRP005332EBI-720116,EBI-297353
FUNDC1Q8IVP53EBI-720116,EBI-3059266
FYCO1Q9BQS82EBI-720116,EBI-2869338
HADHAP409394EBI-720116,EBI-356720
IPO5O004105EBI-720116,EBI-356424
KBTBD6Q86V972EBI-720116,EBI-2514778
KBTBD7Q8WVZ93EBI-720116,EBI-473695
KIAA1958Q8N8K95EBI-720116,EBI-10181113
KRTAP10-7P604093EBI-720116,EBI-10172290
KRTAP10-9P604113EBI-720116,EBI-10172052
MLXQ9UH924EBI-720116,EBI-741109
MLXQ9UH92-34EBI-720116,EBI-8852072
NBR1Q1459611EBI-720116,EBI-742698
NEDD4P469346EBI-720116,EBI-726944
NEK9Q8TD197EBI-720116,EBI-1044009
NIPSNAP2O753236EBI-720116,EBI-307133
PCM1Q151542EBI-720116,EBI-741421
RASSF1Q9NS232EBI-720116,EBI-367363
RASSF5Q8WWW02EBI-720116,EBI-367390
RCN2Q142575EBI-720116,EBI-356710
RETREG1Q9H6L5-12EBI-720116,EBI-16159046
SIK2Q9H0K13EBI-720116,EBI-1181664
SQSTM1Q1350118EBI-720116,EBI-307104
STBD1O952105EBI-720116,EBI-2947137
STK3Q131882EBI-720116,EBI-992580
STK4Q130432EBI-720116,EBI-367376
TBC1D15Q8TC072EBI-720116,EBI-1048247
TBC1D2BQ9UPU73EBI-720116,EBI-2947180
TBC1D5B9A6K13EBI-720116,EBI-10217641
TBC1D5Q926093EBI-720116,EBI-742381
TBC1D9BQ66K14-23EBI-720116,EBI-10217736
TECPR2O150402EBI-720116,EBI-2946991
TNIP1Q150255EBI-720116,EBI-357849
TP53INP1Q96A564EBI-720116,EBI-9986117
TSR2Q969E89EBI-720116,EBI-746981
UBA5Q9GZZ915EBI-720116,EBI-747805
ULK1O753853EBI-720116,EBI-908831
WDFY3Q8IZQ13EBI-720116,EBI-1569256

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116473, 84 interactors
DIPiDIP-35051N
ELMiP60520
IntActiP60520, 529 interactors
MINTiP60520
STRINGi9606.ENSP00000037243

Structurei

Secondary structure

1117
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP60520
SMRiP60520
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 22Interaction with beta-tubulinBy similarityAdd BLAST22
Regioni36 – 68Interaction with GABRG2Sequence analysisAdd BLAST33

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiKOG1654 Eukaryota
ENOG4111JAT LUCA
GeneTreeiENSGT00390000012937
HOGENOMiHOG000232034
HOVERGENiHBG051706
InParanoidiP60520
KOiK08341
OMAiAAAMKWM
OrthoDBiEOG091G10QX
PhylomeDBiP60520
TreeFamiTF312964

Family and domain databases

CDDicd01611 GABARAP, 1 hit
InterProiView protein in InterPro
IPR004241 Atg8-like
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR10969 PTHR10969, 1 hit
PfamiView protein in Pfam
PF02991 Atg8, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P60520-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKWMFKEDHS LEHRCVESAK IRAKYPDRVP VIVEKVSGSQ IVDIDKRKYL
60 70 80 90 100
VPSDITVAQF MWIIRKRIQL PSEKAIFLFV DKTVPQSSLT MGQLYEKEKD
110
EDGFLYVAYS GENTFGF
Length:117
Mass (Da):13,667
Last modified:March 1, 2004 - v1
Checksum:i17ACB540FD5B975B
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BQ50H3BQ50_HUMAN
Gamma-aminobutyric acid receptor-as...
GABARAPL2
57Annotation score:
H3BSM5H3BSM5_HUMAN
Gamma-aminobutyric acid receptor-as...
GABARAPL2
88Annotation score:
H3BU36H3BU36_HUMAN
GABA(A) receptor-associated protein...
GABARAPL2 hCG_21766
42Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29V → F in AAD27779 (Ref. 4) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04975651V → A. Corresponds to variant dbSNP:rs11556291Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030710 mRNA Translation: BAB21548.1
AF087848 mRNA Translation: AAK20400.1
AJ010569 mRNA Translation: CAA09249.1
AF077046 mRNA Translation: AAD27779.1
CR542217 mRNA Translation: CAG47013.1
AK289788 mRNA Translation: BAF82477.1
CH471114 Genomic DNA Translation: EAW95630.1
BC005985 mRNA Translation: AAH05985.1
BC014594 mRNA Translation: AAH14594.1
BC029601 mRNA Translation: AAH29601.1
CCDSiCCDS10921.1
RefSeqiNP_009216.1, NM_007285.6
UniGeneiHs.461379

Genome annotation databases

EnsembliENST00000037243; ENSP00000037243; ENSG00000034713
GeneIDi11345
KEGGihsa:11345
UCSCiuc002fen.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030710 mRNA Translation: BAB21548.1
AF087848 mRNA Translation: AAK20400.1
AJ010569 mRNA Translation: CAA09249.1
AF077046 mRNA Translation: AAD27779.1
CR542217 mRNA Translation: CAG47013.1
AK289788 mRNA Translation: BAF82477.1
CH471114 Genomic DNA Translation: EAW95630.1
BC005985 mRNA Translation: AAH05985.1
BC014594 mRNA Translation: AAH14594.1
BC029601 mRNA Translation: AAH29601.1
CCDSiCCDS10921.1
RefSeqiNP_009216.1, NM_007285.6
UniGeneiHs.461379

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CO7X-ray2.00A/B1-117[»]
ProteinModelPortaliP60520
SMRiP60520
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116473, 84 interactors
DIPiDIP-35051N
ELMiP60520
IntActiP60520, 529 interactors
MINTiP60520
STRINGi9606.ENSP00000037243

Protein family/group databases

MoonDBiP60520 Predicted

PTM databases

iPTMnetiP60520
PhosphoSitePlusiP60520

Polymorphism and mutation databases

BioMutaiGABARAPL2
DMDMi44888808

2D gel databases

UCD-2DPAGEiP60520

Proteomic databases

EPDiP60520
PaxDbiP60520
PeptideAtlasiP60520
PRIDEiP60520
ProteomicsDBi57214

Protocols and materials databases

DNASUi11345
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000037243; ENSP00000037243; ENSG00000034713
GeneIDi11345
KEGGihsa:11345
UCSCiuc002fen.3 human

Organism-specific databases

CTDi11345
DisGeNETi11345
EuPathDBiHostDB:ENSG00000034713.7
GeneCardsiGABARAPL2
HGNCiHGNC:13291 GABARAPL2
HPAiHPA036726
MIMi607452 gene
neXtProtiNX_P60520
OpenTargetsiENSG00000034713
PharmGKBiPA28482
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1654 Eukaryota
ENOG4111JAT LUCA
GeneTreeiENSGT00390000012937
HOGENOMiHOG000232034
HOVERGENiHBG051706
InParanoidiP60520
KOiK08341
OMAiAAAMKWM
OrthoDBiEOG091G10QX
PhylomeDBiP60520
TreeFamiTF312964

Enzyme and pathway databases

ReactomeiR-HSA-1632852 Macroautophagy
R-HSA-8854214 TBC/RABGAPs
SIGNORiP60520

Miscellaneous databases

ChiTaRSiGABARAPL2 human
GeneWikiiGABARAPL2
GenomeRNAii11345
PMAP-CutDBiP60520
PROiPR:P60520
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000034713 Expressed in 238 organ(s), highest expression level in caudate nucleus
CleanExiHS_GABARAPL2
ExpressionAtlasiP60520 baseline and differential
GenevisibleiP60520 HS

Family and domain databases

CDDicd01611 GABARAP, 1 hit
InterProiView protein in InterPro
IPR004241 Atg8-like
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR10969 PTHR10969, 1 hit
PfamiView protein in Pfam
PF02991 Atg8, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGBRL2_HUMAN
AccessioniPrimary (citable) accession number: P60520
Secondary accession number(s): O08765
, Q6FG91, Q9DCP8, Q9UQF7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: September 12, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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