UniProtKB - P60513 (U6A_CONER)
Protein
Delta-conotoxin EVIA
Gene
N/A
Organism
Conus ermineus (Atlantic fish-hunting cone)
Status
Functioni
Delta-conotoxins bind to site 6 of voltage-gated sodium channels and inhibit the inactivation process. This toxin inhibits sodium channel inactivation in neuronal membranes from amphibians and mammals (Nav1.2a/SCN1A, Nav1.3/SCN3A and Nav1.6/SCN8A) upon binding to receptor site 6.1 Publication
Miscellaneous
Does not affect rat skeletal muscle (Nav1.4/SCN4A) and human cardiac muscle (Nav1.5/SCN5A) sodium channels.1 Publication
Exists in two forms, due to cis-trans isomerization at 12-Leu-Pro-13.1 Publication
Caution
Another delta-conotoxin was named EVIA in 2001, but this toxin was renamed EVIB (AC P69752).Curated
GO - Molecular functioni
- sodium channel inhibitor activity Source: InterPro
- toxin activity Source: UniProtKB-KW
Keywordsi
Molecular function | Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin |
Names & Taxonomyi
Protein namesi | Recommended name: Delta-conotoxin EVIA2 PublicationsShort name: Delta-EVIA2 Publications |
Organismi | Conus ermineus (Atlantic fish-hunting cone) |
Taxonomic identifieri | 55423 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Lophotrochozoa › Mollusca › Gastropoda › Caenogastropoda › Neogastropoda › Conoidea › Conidae › Conus › Chelyconus |
Organism-specific databases
ConoServeri | 1561 EVIA |
Subcellular locationi
Extracellular region or secreted
- Secreted 2 Publications
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Toxic dosei
Dose that produces hyperactivity (ED(50)) is 1.8 pmol/g body mass (native toxin), 1.9 pmol/g body mass (synthetic toxin) and 3.9 pmol/g body mass (P13A mutant).1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 13 | P → A: 2-fold decrease of activity. Exists only as a trans isomer. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PeptideiPRO_0000044868 | 1 – 32 | Delta-conotoxin EVIA1 PublicationAdd BLAST | 32 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 3 ↔ 21 | 1 PublicationImported | ||
Modified residuei | 6 | 4-hydroxyproline1 Publication | 1 | |
Disulfide bondi | 10 ↔ 25 | 1 PublicationImported | ||
Disulfide bondi | 20 ↔ 29 | 1 PublicationImported | ||
Modified residuei | 32 | Leucine amide1 Publication | 1 |
Keywords - PTMi
Amidation, Disulfide bond, HydroxylationExpressioni
Tissue specificityi
Expressed by the venom duct.2 Publications
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1G1P | NMR | - | A | 1-32 | [»] | |
1G1Z | NMR | - | A | 1-32 | [»] | |
ProteinModelPortali | P60513 | |||||
SMRi | P60513 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P60513 |
Family & Domainsi
Domaini
The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.1 PublicationImported
The cysteine framework is VI/VII (C-C-CC-C-C).Curated
Sequence similaritiesi
Belongs to the conotoxin O1 superfamily.Curated
Keywords - Domaini
KnottinFamily and domain databases
InterProi | View protein in InterPro IPR012322 Conotoxin_d-typ_CS |
PROSITEi | View protein in PROSITE PS60005 DELTA_CONOTOXIN, 1 hit |
i Sequence
Sequence statusi: Complete.
Mass spectrometryi
Similar proteinsi
Cross-referencesi
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1G1P | NMR | - | A | 1-32 | [»] | |
1G1Z | NMR | - | A | 1-32 | [»] | |
ProteinModelPortali | P60513 | |||||
SMRi | P60513 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Organism-specific databases
ConoServeri | 1561 EVIA |
Miscellaneous databases
EvolutionaryTracei | P60513 |
Family and domain databases
InterProi | View protein in InterPro IPR012322 Conotoxin_d-typ_CS |
PROSITEi | View protein in PROSITE PS60005 DELTA_CONOTOXIN, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | U6A_CONER | |
Accessioni | P60513Primary (citable) accession number: P60513 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2004 |
Last sequence update: | March 1, 2004 | |
Last modified: | October 10, 2018 | |
This is version 75 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families - PDB cross-references
Index of Protein Data Bank (PDB) cross-references