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Protein

Ribosomal RNA small subunit methyltransferase H

Gene

rsmH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA. In vitro, active on the assembled 30S subunit, but not naked 16S rRNA or 70S ribosomes.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N4-methylcytosine(1402) in 16S rRNA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei55S-adenosyl-L-methionineBy similarity1
Binding sitei79S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei101S-adenosyl-L-methionineBy similarity1
Binding sitei108S-adenosyl-L-methionineBy similarity1

GO - Molecular functioni

  • rRNA (cytosine-N4-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • rRNA base methylation Source: EcoCyc

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG11085-MONOMER
MetaCyc:EG11085-MONOMER
BRENDAi2.1.1.199 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase H (EC:2.1.1.199)
Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH
Gene namesi
Name:rsmH
Synonyms:mraW, yabC
Ordered Locus Names:b0082, JW0080
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11085 rsmH

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001086201 – 313Ribosomal RNA small subunit methyltransferase HAdd BLAST313

Proteomic databases

EPDiP60390
PaxDbiP60390
PRIDEiP60390

Interactioni

Protein-protein interaction databases

BioGridi4261639, 47 interactors
DIPiDIP-35840N
IntActiP60390, 7 interactors
STRINGi316385.ECDH10B_0064

Structurei

Secondary structure

1313
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP60390
SMRiP60390
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 37S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CGJ Bacteria
COG0275 LUCA
HOGENOMiHOG000049778
InParanoidiP60390
KOiK03438
PhylomeDBiP60390

Family and domain databases

Gene3Di1.10.150.170, 1 hit
HAMAPiMF_01007 16SrRNA_methyltr_H, 1 hit
InterProiView protein in InterPro
IPR002903 RsmH
IPR023397 SAM-dep_MeTrfase_MraW_recog
IPR029063 SAM-dependent_MTases
PANTHERiPTHR11265 PTHR11265, 1 hit
PfamiView protein in Pfam
PF01795 Methyltransf_5, 1 hit
PIRSFiPIRSF004486 MraW, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
SSF81799 SSF81799, 1 hit
TIGRFAMsiTIGR00006 TIGR00006, 1 hit

Sequencei

Sequence statusi: Complete.

P60390-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMENYKHTTV LLDEAVNGLN IRPDGIYIDG TFGRGGHSRL ILSQLGEEGR
60 70 80 90 100
LLAIDRDPQA IAVAKTIDDP RFSIIHGPFS ALGEYVAERD LIGKIDGILL
110 120 130 140 150
DLGVSSPQLD DAERGFSFMR DGPLDMRMDP TRGQSAAEWL QTAEEADIAW
160 170 180 190 200
VLKTYGEERF AKRIARAIVE RNREQPMTRT KELAEVVAAA TPVKDKFKHP
210 220 230 240 250
ATRTFQAVRI WVNSELEEIE QALKSSLNVL APGGRLSIIS FHSLEDRIVK
260 270 280 290 300
RFMRENSRGP QVPAGLPMTE EQLKKLGGRQ LRALGKLMPG EEEVAENPRA
310
RSSVLRIAER TNA
Length:313
Mass (Da):34,878
Last modified:February 16, 2004 - v1
Checksum:i43174C907A36ECC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52063 Genomic DNA Translation: CAA36283.1
X55034 Genomic DNA Translation: CAA38859.1
U00096 Genomic DNA Translation: AAC73193.1
AP009048 Genomic DNA Translation: BAB96650.1
K00137 Genomic DNA No translation available.
PIRiJH0092 QQECFT
RefSeqiNP_414624.1, NC_000913.3
WP_000970479.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73193; AAC73193; b0082
BAB96650; BAB96650; BAB96650
GeneIDi944806
KEGGiecj:JW0080
eco:b0082
PATRICifig|1411691.4.peg.2198

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52063 Genomic DNA Translation: CAA36283.1
X55034 Genomic DNA Translation: CAA38859.1
U00096 Genomic DNA Translation: AAC73193.1
AP009048 Genomic DNA Translation: BAB96650.1
K00137 Genomic DNA No translation available.
PIRiJH0092 QQECFT
RefSeqiNP_414624.1, NC_000913.3
WP_000970479.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TKAX-ray2.25A1-313[»]
ProteinModelPortaliP60390
SMRiP60390
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261639, 47 interactors
DIPiDIP-35840N
IntActiP60390, 7 interactors
STRINGi316385.ECDH10B_0064

Proteomic databases

EPDiP60390
PaxDbiP60390
PRIDEiP60390

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73193; AAC73193; b0082
BAB96650; BAB96650; BAB96650
GeneIDi944806
KEGGiecj:JW0080
eco:b0082
PATRICifig|1411691.4.peg.2198

Organism-specific databases

EchoBASEiEB1077
EcoGeneiEG11085 rsmH

Phylogenomic databases

eggNOGiENOG4105CGJ Bacteria
COG0275 LUCA
HOGENOMiHOG000049778
InParanoidiP60390
KOiK03438
PhylomeDBiP60390

Enzyme and pathway databases

BioCyciEcoCyc:EG11085-MONOMER
MetaCyc:EG11085-MONOMER
BRENDAi2.1.1.199 2026

Miscellaneous databases

PROiPR:P60390

Family and domain databases

Gene3Di1.10.150.170, 1 hit
HAMAPiMF_01007 16SrRNA_methyltr_H, 1 hit
InterProiView protein in InterPro
IPR002903 RsmH
IPR023397 SAM-dep_MeTrfase_MraW_recog
IPR029063 SAM-dependent_MTases
PANTHERiPTHR11265 PTHR11265, 1 hit
PfamiView protein in Pfam
PF01795 Methyltransf_5, 1 hit
PIRSFiPIRSF004486 MraW, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
SSF81799 SSF81799, 1 hit
TIGRFAMsiTIGR00006 TIGR00006, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRSMH_ECOLI
AccessioniPrimary (citable) accession number: P60390
Secondary accession number(s): P18595
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: February 16, 2004
Last modified: November 7, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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