UniProtKB - P60340 (TRUB_ECOLI)
Protein
tRNA pseudouridine synthase B
Gene
truB
Organism
Escherichia coli (strain K12)
Status
Functioni
Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.UniRule annotation2 Publications
Catalytic activityi
- EC:5.4.99.25UniRule annotation1 Publication
Kineticsi
- KM=148 nM for tRNA (at pH 7.5)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 43 | SubstrateUniRule annotation1 Publication | 1 | |
Active sitei | 48 | NucleophileUniRule annotation1 Publication | 1 | |
Binding sitei | 76 | SubstrateUniRule annotation1 Publication | 1 | |
Binding sitei | 179 | SubstrateUniRule annotation1 Publication | 1 | |
Binding sitei | 202 | SubstrateUniRule annotation1 Publication | 1 |
GO - Molecular functioni
- pseudouridine synthase activity Source: GO_Central
- tRNA binding Source: EcoCyc
- tRNA pseudouridine synthase activity Source: EcoCyc
GO - Biological processi
- mRNA pseudouridine synthesis Source: GO_Central
- tRNA folding Source: EcoCyc
- tRNA modification Source: GO_Central
- tRNA pseudouridine synthesis Source: EcoCyc
Keywordsi
Molecular function | Isomerase |
Biological process | tRNA processing |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11177-MONOMER MetaCyc:EG11177-MONOMER |
BRENDAi | 5.4.99.25, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA pseudouridine synthase BUniRule annotation (EC:5.4.99.25UniRule annotation1 Publication)Alternative name(s): Protein P351 Publication tRNA pseudouridine(55) synthaseUniRule annotation Short name: Psi55 synthaseUniRule annotation tRNA pseudouridylate synthaseUniRule annotation tRNA-uridine isomeraseUniRule annotation |
Gene namesi | Name:truB Synonyms:yhbA Ordered Locus Names:b3166, JW3135 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 19 | K → M or R: Reduced structural stability and decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 20 | P → G or L: Reduced structural stability and no change in activity. 1 Publication | 1 | |
Mutagenesisi | 43 | H → A: 330-fold decrease in catalytic efficiency. 1 Publication | 1 | |
Mutagenesisi | 43 | H → F: 2-fold decrease in catalytic efficiency. 1 Publication | 1 | |
Mutagenesisi | 43 | H → G: 250-fold decrease in catalytic efficiency. 1 Publication | 1 | |
Mutagenesisi | 43 | H → N: 180-fold decrease in catalytic efficiency. 1 Publication | 1 | |
Mutagenesisi | 43 | H → Q: 50-fold decrease in catalytic efficiency. 1 Publication | 1 | |
Mutagenesisi | 48 | D → C: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 58 | C → A: Slight increase in activity. Slight increase in activity; when associated with A-174 and A-193. 1 Publication | 1 | |
Mutagenesisi | 174 | C → A: Slight increase in activity. Slight increase in activity; when associated with A-58 and A-193. 1 Publication | 1 | |
Mutagenesisi | 193 | C → A: Slight increase in activity; when associated with A-58 and A-174. 1 Publication | 1 | |
Mutagenesisi | 193 | C → V: Slight increase in activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000121830 | 1 – 314 | tRNA pseudouridine synthase BAdd BLAST | 314 |
Proteomic databases
jPOSTi | P60340 |
PaxDbi | P60340 |
PRIDEi | P60340 |
Expressioni
Inductioni
Weakly expressed in maxicells, part of the metY operon that extends to pnp (PubMed:2849753).1 Publication
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P60340 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P60340 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 124 – 152 | RNA bindingBy similarityAdd BLAST | 29 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0130, Bacteria |
HOGENOMi | CLU_032087_0_3_6 |
InParanoidi | P60340 |
PhylomeDBi | P60340 |
Family and domain databases
CDDi | cd02573, PseudoU_synth_EcTruB, 1 hit |
Gene3Di | 2.30.130.10, 1 hit |
HAMAPi | MF_01080, TruB_bact, 1 hit |
InterProi | View protein in InterPro IPR020103, PsdUridine_synth_cat_dom_sf IPR002501, PsdUridine_synth_N IPR015947, PUA-like_sf IPR036974, PUA_sf IPR014780, tRNA_psdUridine_synth_TruB IPR015240, tRNA_sdUridine_synth_fam1_C IPR032819, TruB_C |
PANTHERi | PTHR13767, PTHR13767, 1 hit |
Pfami | View protein in Pfam PF09157, TruB-C_2, 1 hit PF16198, TruB_C_2, 1 hit PF01509, TruB_N, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit SSF88697, SSF88697, 1 hit |
TIGRFAMsi | TIGR00431, TruB, 1 hit |
i Sequence
Sequence statusi: Complete.
P60340-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRPRRRGRD INGVLLLDKP QGMSSNDALQ KVKRIYNANR AGHTGALDPL
60 70 80 90 100
ATGMLPICLG EATKFSQYLL DSDKRYRVIA RLGQRTDTSD ADGQIVEERP
110 120 130 140 150
VTFSAEQLAA ALDTFRGDIE QIPSMYSALK YQGKKLYEYA RQGIEVPREA
160 170 180 190 200
RPITVYELLF IRHEGNELEL EIHCSKGTYI RTIIDDLGEK LGCGAHVIYL
210 220 230 240 250
RRLAVSKYPV ERMVTLEHLR ELVEQAEQQD IPAAELLDPL LMPMDSPASD
260 270 280 290 300
YPVVNLPLTS SVYFKNGNPV RTSGAPLEGL VRVTEGENGK FIGMGEIDDE
310
GRVAPRRLVV EYPA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13270 Genomic DNA Translation: CAA31635.1 X13775 Genomic DNA Translation: CAA32021.1 U18997 Genomic DNA Translation: AAA57969.1 U00096 Genomic DNA Translation: AAC76200.1 AP009048 Genomic DNA Translation: BAE77212.1 S79856 Genomic DNA Translation: AAB35645.1 |
PIRi | S01916, Q9EC35 |
RefSeqi | NP_417635.1, NC_000913.3 WP_000089698.1, NZ_STEB01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC76200; AAC76200; b3166 BAE77212; BAE77212; BAE77212 |
GeneIDi | 58348663 947687 |
KEGGi | ecj:JW3135 eco:b3166 |
PATRICi | fig|1411691.4.peg.3564 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13270 Genomic DNA Translation: CAA31635.1 X13775 Genomic DNA Translation: CAA32021.1 U18997 Genomic DNA Translation: AAA57969.1 U00096 Genomic DNA Translation: AAC76200.1 AP009048 Genomic DNA Translation: BAE77212.1 S79856 Genomic DNA Translation: AAB35645.1 |
PIRi | S01916, Q9EC35 |
RefSeqi | NP_417635.1, NC_000913.3 WP_000089698.1, NZ_STEB01000012.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1K8W | X-ray | 1.85 | A | 7-314 | [»] | |
1R3F | X-ray | 1.85 | A | 1-314 | [»] | |
1ZL3 | X-ray | 2.80 | A | 10-314 | [»] | |
SMRi | P60340 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P60340, 5 interactors |
STRINGi | 511145.b3166 |
Proteomic databases
jPOSTi | P60340 |
PaxDbi | P60340 |
PRIDEi | P60340 |
Genome annotation databases
EnsemblBacteriai | AAC76200; AAC76200; b3166 BAE77212; BAE77212; BAE77212 |
GeneIDi | 58348663 947687 |
KEGGi | ecj:JW3135 eco:b3166 |
PATRICi | fig|1411691.4.peg.3564 |
Organism-specific databases
EchoBASEi | EB1164 |
Phylogenomic databases
eggNOGi | COG0130, Bacteria |
HOGENOMi | CLU_032087_0_3_6 |
InParanoidi | P60340 |
PhylomeDBi | P60340 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11177-MONOMER MetaCyc:EG11177-MONOMER |
BRENDAi | 5.4.99.25, 2026 |
Miscellaneous databases
EvolutionaryTracei | P60340 |
PROi | PR:P60340 |
Family and domain databases
CDDi | cd02573, PseudoU_synth_EcTruB, 1 hit |
Gene3Di | 2.30.130.10, 1 hit |
HAMAPi | MF_01080, TruB_bact, 1 hit |
InterProi | View protein in InterPro IPR020103, PsdUridine_synth_cat_dom_sf IPR002501, PsdUridine_synth_N IPR015947, PUA-like_sf IPR036974, PUA_sf IPR014780, tRNA_psdUridine_synth_TruB IPR015240, tRNA_sdUridine_synth_fam1_C IPR032819, TruB_C |
PANTHERi | PTHR13767, PTHR13767, 1 hit |
Pfami | View protein in Pfam PF09157, TruB-C_2, 1 hit PF16198, TruB_C_2, 1 hit PF01509, TruB_N, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit SSF88697, SSF88697, 1 hit |
TIGRFAMsi | TIGR00431, TruB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRUB_ECOLI | |
Accessioni | P60340Primary (citable) accession number: P60340 Secondary accession number(s): P09171, P76671, Q2M944 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 16, 2004 |
Last sequence update: | February 16, 2004 | |
Last modified: | April 7, 2021 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families