UniProtKB - P60301 (3SA3_NAJAT)
Cytotoxin 3
Functioni
Basic protein that binds to cell membrane and depolarizes cardiomyocytes. This cytotoxin also possesses lytic activity on many other cells, including red blood cells (PubMed:8182052).
Interaction with sulfatides in the cell membrane induces pore formation and cell internalization. Cytotoxicity is due to pore formation, and to another mechanism independent of membrane-damaging activity. When internalized, it targets the mitochondrial membrane and induces mitochondrial swelling and fragmentation. It inhibits protein kinases C. It binds to the integrin alpha-V/beta-3 (ITGAV/ITGB3) with a moderate affinity (PubMed:16407244).
It also binds with high affinity to heparin (PubMed:17685633).
7 PublicationsMiscellaneous
GO - Molecular functioni
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- hemolysis in another organism Source: UniProtKB-KW
Keywordsi
Molecular function | Cardiotoxin, Toxin |
Biological process | Cytolysis, Hemolysis |
Names & Taxonomyi
Protein namesi | Recommended name: Cytotoxin 3Alternative name(s): Cardiotoxin 31 Publication Short name: CTX-3 Short name: CTX3 Cardiotoxin A32 Publications Short name: CTX A32 Publications Cardiotoxin III1 Publication Cardiotoxin analog III Short name: CTX III |
Organismi | Naja atra (Chinese cobra) |
Taxonomic identifieri | 8656 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Serpentes › Colubroidea › Elapidae › Elapinae › Naja |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Other locations
- Target cell membrane 2 Publications
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Other locations
- membrane Source: UniProtKB-KW
Keywords - Cellular componenti
Membrane, Secreted, Target cell membrane, Target membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | 3 PublicationsAdd BLAST | 21 | |
ChainiPRO_0000035370 | 22 – 81 | Cytotoxin 33 PublicationsAdd BLAST | 60 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 24 ↔ 42 | 4 PublicationsImported | ||
Disulfide bondi | 35 ↔ 59 | 4 PublicationsImported | ||
Disulfide bondi | 63 ↔ 74 | 4 PublicationsImported | ||
Disulfide bondi | 75 ↔ 80 | 4 PublicationsImported |
Keywords - PTMi
Disulfide bondExpressioni
Tissue specificityi
Interactioni
Subunit structurei
Monomer in solution; Homodimer and oligomer in the presence of negatively charged lipids forming a pore with a size ranging between 20 and 30 Angstroms (PubMed:16263708,PubMed:12660250).
Interacts with Kv channel-interacting protein 1 (KCNIP1) in a calcium-independent manner (PubMed:15184042).
3 PublicationsStructurei
Secondary structure
3D structure databases
AlphaFoldDBi | P60301 |
BMRBi | P60301 |
SMRi | P60301 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P60301 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalFamily and domain databases
CDDi | cd00206, snake_toxin, 1 hit |
Gene3Di | 2.10.60.10, 1 hit |
InterProi | View protein in InterPro IPR003572, Cytotoxin_Cobra IPR003571, Snake_3FTx IPR045860, Snake_toxin-like_sf IPR018354, Snake_toxin_con_site |
PRINTSi | PR00282, CYTOTOXIN |
SUPFAMi | SSF57302, SSF57302, 1 hit |
PROSITEi | View protein in PROSITE PS00272, SNAKE_TOXIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MKTLLLTLVV VTIVCLDLGY TLKCNKLVPL FYKTCPAGKN LCYKMFMVAT
60 70 80
PKVPVKRGCI DVCPKSSLLV KYVCCNTDRC N
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 9 – 11 | VVV → EEE in CAB42055 (PubMed:10708798).Curated | 3 | |
Sequence conflicti | 59 | C → R in CAA90963 (PubMed:8619792).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z54227 mRNA Translation: CAA90963.1 U58486 mRNA Translation: AAB18382.1 U42585 mRNA Translation: AAB01541.1 AJ007796 mRNA Translation: CAA07686.1 AJ238735 Genomic DNA Translation: CAB42055.1 |
PIRi | JC4620, H3NJ3F |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z54227 mRNA Translation: CAA90963.1 U58486 mRNA Translation: AAB18382.1 U42585 mRNA Translation: AAB01541.1 AJ007796 mRNA Translation: CAA07686.1 AJ238735 Genomic DNA Translation: CAB42055.1 |
PIRi | JC4620, H3NJ3F |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1H0J | X-ray | 1.90 | A/B/C | 22-81 | [»] | |
1I02 | NMR | - | A | 22-81 | [»] | |
1XT3 | X-ray | 2.40 | A/B | 22-81 | [»] | |
2BHI | X-ray | 2.31 | A/B | 22-81 | [»] | |
2CRS | NMR | - | A | 22-81 | [»] | |
2CRT | NMR | - | A | 22-81 | [»] | |
AlphaFoldDBi | P60301 | |||||
BMRBi | P60301 | |||||
SMRi | P60301 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P60301 |
Family and domain databases
CDDi | cd00206, snake_toxin, 1 hit |
Gene3Di | 2.10.60.10, 1 hit |
InterProi | View protein in InterPro IPR003572, Cytotoxin_Cobra IPR003571, Snake_3FTx IPR045860, Snake_toxin-like_sf IPR018354, Snake_toxin_con_site |
PRINTSi | PR00282, CYTOTOXIN |
SUPFAMi | SSF57302, SSF57302, 1 hit |
PROSITEi | View protein in PROSITE PS00272, SNAKE_TOXIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | 3SA3_NAJAT | |
Accessioni | P60301Primary (citable) accession number: P60301 Secondary accession number(s): P01444, Q9PS23, Q9W6W7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | February 2, 2004 | |
Last modified: | May 25, 2022 | |
This is version 90 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Annotation program | Animal Toxin Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families