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Protein

Cytotoxin 3

Gene
N/A
Organism
Naja atra (Chinese cobra)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Basic protein that binds to cell membrane and depolarizes cardiomyocytes. This cytotoxin also possesses lytic activity on many other cells, including red blood cells (PubMed:8182052). Interaction with sulfatides in the cell membrane induces pore formation and cell internalization. Cytotoxicity is due to pore formation, and to another mechanism independent of membrane-damaging activity. When internalized, it targets the mitochondrial membrane and induces mitochondrial swelling and fragmentation. It inhibits protein kinases C. It binds to the integrin alpha-V/beta-3 (ITGAV/ITGB3) with a moderate affinity (PubMed:16407244). It also binds with high affinity to heparin (PubMed:17685633).7 Publications

Miscellaneous

Is classified as a P-type cytotoxin, since a proline residue stands at position 30 (Pro-31 in standard classification).1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCardiotoxin, Toxin
Biological processCytolysis, Hemolysis

Names & Taxonomyi

Protein namesi
Recommended name:
Cytotoxin 3
Alternative name(s):
Cardiotoxin 31 Publication
Short name:
CTX-3
Short name:
CTX3
Cardiotoxin A32 Publications
Short name:
CTX A32 Publications
Cardiotoxin III1 Publication
Cardiotoxin analog III
Short name:
CTX III
OrganismiNaja atra (Chinese cobra)
Taxonomic identifieri8656 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeNaja

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Secreted, Target cell membrane, Target membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 213 PublicationsAdd BLAST21
ChainiPRO_000003537022 – 81Cytotoxin 3Add BLAST60

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi24 ↔ 424 Publications
Disulfide bondi35 ↔ 594 Publications
Disulfide bondi63 ↔ 744 Publications
Disulfide bondi75 ↔ 804 Publications

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.Curated

Interactioni

Subunit structurei

Monomer in solution; Homodimer and oligomer in the presence of negatively charged lipids forming a pore with a size ranging between 20 and 30 Angstroms (PubMed:16263708,PubMed:12660250). Interacts with Kv channel-interacting protein 1 (KCNIP1) in a calcium-independent manner (PubMed:15184042).3 Publications

Structurei

Secondary structure

181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP60301
SMRiP60301
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60301

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006553

Family and domain databases

CDDicd00206 snake_toxin, 1 hit
InterProiView protein in InterPro
IPR003572 Cytotoxin_Cobra
IPR003571 Snake_3FTx
IPR018354 Snake_toxin_con_site
IPR035076 Toxin/TOLIP
PfamiView protein in Pfam
PF00087 Toxin_TOLIP, 1 hit
PRINTSiPR00282 CYTOTOXIN
PROSITEiView protein in PROSITE
PS00272 SNAKE_TOXIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60301-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKTLLLTLVV VTIVCLDLGY TLKCNKLVPL FYKTCPAGKN LCYKMFMVAT
60 70 80
PKVPVKRGCI DVCPKSSLLV KYVCCNTDRC N
Length:81
Mass (Da):9,038
Last modified:February 2, 2004 - v1
Checksum:i70FE0A82FAC9DC95
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9 – 11VVV → EEE in CAB42055 (PubMed:10708798).Curated3
Sequence conflicti59C → R in CAA90963 (PubMed:8619792).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54227 mRNA Translation: CAA90963.1
U58486 mRNA Translation: AAB18382.1
U42585 mRNA Translation: AAB01541.1
AJ007796 mRNA Translation: CAA07686.1
AJ238735 Genomic DNA Translation: CAB42055.1
PIRiJC4620 H3NJ3F

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54227 mRNA Translation: CAA90963.1
U58486 mRNA Translation: AAB18382.1
U42585 mRNA Translation: AAB01541.1
AJ007796 mRNA Translation: CAA07686.1
AJ238735 Genomic DNA Translation: CAB42055.1
PIRiJC4620 H3NJ3F

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H0JX-ray1.90A/B/C22-81[»]
1I02NMR-A22-81[»]
1XT3X-ray2.40A/B22-81[»]
2BHIX-ray2.31A/B22-81[»]
2CRSNMR-A22-81[»]
2CRTNMR-A22-81[»]
ProteinModelPortaliP60301
SMRiP60301
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG006553

Miscellaneous databases

EvolutionaryTraceiP60301

Family and domain databases

CDDicd00206 snake_toxin, 1 hit
InterProiView protein in InterPro
IPR003572 Cytotoxin_Cobra
IPR003571 Snake_3FTx
IPR018354 Snake_toxin_con_site
IPR035076 Toxin/TOLIP
PfamiView protein in Pfam
PF00087 Toxin_TOLIP, 1 hit
PRINTSiPR00282 CYTOTOXIN
PROSITEiView protein in PROSITE
PS00272 SNAKE_TOXIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei3SA3_NAJAT
AccessioniPrimary (citable) accession number: P60301
Secondary accession number(s): P01444, Q9PS23, Q9W6W7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 2, 2004
Last modified: May 23, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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