UniProtKB - P60293 (MUKF_ECOLI)
Protein
Chromosome partition protein MukF
Gene
mukF
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in chromosome condensation, segregation and cell cycle progression. May participate in facilitating chromosome segregation by condensation DNA from both sides of a centrally located replisome during cell division. Not required for mini-F plasmid partitioning. Probably acts via its interaction with MukB and MukE. Overexpression results in anucleate cells. It has a calcium binding activity.1 Publication
Caution
Was originally thought to be a killing factor. PubMed:8602138 showed that it is not involved in killing system.Curated
GO - Molecular functioni
- calcium ion binding Source: UniProtKB-UniRule
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- chromosome condensation Source: UniProtKB-KW
- chromosome segregation Source: UniProtKB-UniRule
- DNA replication Source: UniProtKB-UniRule
Keywordsi
Biological process | Cell cycle, Cell division, Chromosome partition, DNA condensation |
Ligand | Calcium |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12165-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Chromosome partition protein MukFAlternative name(s): Protein KicB |
Gene namesi | Name:mukF Synonyms:kicB Ordered Locus Names:b0922, JW0905 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- nucleoid Note: Restricted to the nucleoid region.
GO - Cellular componenti
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 233 | L → P: Abolishes function. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000211600 | 1 – 440 | Chromosome partition protein MukFAdd BLAST | 440 |
Proteomic databases
jPOSTi | P60293 |
PaxDbi | P60293 |
PRIDEi | P60293 |
Interactioni
Subunit structurei
Interacts, and probably forms a ternary complex, with MukE and MukB via its C-terminal region. The complex formation is stimulated by calcium or magnesium. It is required for an interaction between MukE and MukB.
2 PublicationsBinary interactionsi
P60293
With | #Exp. | IntAct |
---|---|---|
mukE [P22524] | 6 | EBI-554679,EBI-554672 |
Protein-protein interaction databases
BioGRIDi | 4260007, 248 interactors |
ComplexPortali | CPX-1090, MukBEF condensin complex |
DIPi | DIP-39981N |
IntActi | P60293, 3 interactors |
MINTi | P60293 |
STRINGi | 511145.b0922 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P60293 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P60293 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 208 – 236 | Leucine-zipperAdd BLAST | 29 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 325 – 356 | Asp/Glu-rich (acidic)Add BLAST | 32 |
Sequence similaritiesi
Belongs to the MukF family.Curated
Phylogenomic databases
eggNOGi | COG3006, Bacteria |
HOGENOMi | CLU_049853_0_0_6 |
Family and domain databases
CDDi | cd16337, MukF_C, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 1.10.225.40, 1 hit 1.20.58.590, 1 hit |
HAMAPi | MF_01803, MukF, 1 hit |
InterProi | View protein in InterPro IPR005582, Chromosome_partition_MukF IPR033441, MukF_C IPR038198, MukF_C_sf IPR033440, MukF_M IPR036141, MukF_M_sp IPR033439, MukF_WHTH IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF03882, KicB, 1 hit PF17193, MukF_C, 1 hit PF17192, MukF_M, 1 hit |
PIRSFi | PIRSF018282, MukF, 1 hit |
SUPFAMi | SSF140570, SSF140570, 1 hit SSF46785, SSF46785, 1 hit |
i Sequence
Sequence statusi: Complete.
P60293-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEFSQTVPE LVAWARKNDF SISLPVDRLS FLLAVATLNG ERLDGEMSEG
60 70 80 90 100
ELVDAFRHVS DAFEQTSETI GVRANNAIND MVRQRLLNRF TSEQAEGNAI
110 120 130 140 150
YRLTPLGIGI TDYYIRQREF STLRLSMQLS IVAGELKRAA DAAEEGGDEF
160 170 180 190 200
HWHRNVYAPL KYSVAEIFDS IDLTQRLMDE QQQQVKDDIA QLLNKDWRAA
210 220 230 240 250
ISSCELLLSE TSGTLRELQD TLEAAGDKLQ ANLLRIQDAT MTHDDLHFVD
260 270 280 290 300
RLVFDLQSKL DRIISWGQQS IDLWIGYDRH VHKFIRTAID MDKNRVFAQR
310 320 330 340 350
LRQSVQTYFD EPWALTYANA DRLLDMRDEE MALRDEEVTG ELPEDLEYEE
360 370 380 390 400
FNEIREQLAA IIEEQLAVYK TRQVPLDLGL VVREYLSQYP RARHFDVARI
410 420 430 440
VIDQAVRLGV AQADFTGLPA KWQPINDYGA KVQAHVIDKY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26440 Genomic DNA Translation: BAA05457.1 U00096 Genomic DNA Translation: AAC74008.1 AP009048 Genomic DNA Translation: BAA35668.1 |
PIRi | S43911 |
RefSeqi | NP_415442.1, NC_000913.3 WP_001288850.1, NZ_STEB01000006.1 |
Genome annotation databases
EnsemblBacteriai | AAC74008; AAC74008; b0922 BAA35668; BAA35668; BAA35668 |
GeneIDi | 48724794 945548 |
KEGGi | ecj:JW0905 eco:b0922 |
PATRICi | fig|1411691.4.peg.1354 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26440 Genomic DNA Translation: BAA05457.1 U00096 Genomic DNA Translation: AAC74008.1 AP009048 Genomic DNA Translation: BAA35668.1 |
PIRi | S43911 |
RefSeqi | NP_415442.1, NC_000913.3 WP_001288850.1, NZ_STEB01000006.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1T98 | X-ray | 2.90 | A/B | 1-287 | [»] | |
3EUH | X-ray | 2.90 | A/B | 1-440 | [»] | |
3RPU | X-ray | 3.60 | A/B/X | 1-440 | [»] | |
SMRi | P60293 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260007, 248 interactors |
ComplexPortali | CPX-1090, MukBEF condensin complex |
DIPi | DIP-39981N |
IntActi | P60293, 3 interactors |
MINTi | P60293 |
STRINGi | 511145.b0922 |
Proteomic databases
jPOSTi | P60293 |
PaxDbi | P60293 |
PRIDEi | P60293 |
Genome annotation databases
EnsemblBacteriai | AAC74008; AAC74008; b0922 BAA35668; BAA35668; BAA35668 |
GeneIDi | 48724794 945548 |
KEGGi | ecj:JW0905 eco:b0922 |
PATRICi | fig|1411691.4.peg.1354 |
Organism-specific databases
EchoBASEi | EB2084 |
Phylogenomic databases
eggNOGi | COG3006, Bacteria |
HOGENOMi | CLU_049853_0_0_6 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12165-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P60293 |
PROi | PR:P60293 |
Family and domain databases
CDDi | cd16337, MukF_C, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 1.10.225.40, 1 hit 1.20.58.590, 1 hit |
HAMAPi | MF_01803, MukF, 1 hit |
InterProi | View protein in InterPro IPR005582, Chromosome_partition_MukF IPR033441, MukF_C IPR038198, MukF_C_sf IPR033440, MukF_M IPR036141, MukF_M_sp IPR033439, MukF_WHTH IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF03882, KicB, 1 hit PF17193, MukF_C, 1 hit PF17192, MukF_M, 1 hit |
PIRSFi | PIRSF018282, MukF, 1 hit |
SUPFAMi | SSF140570, SSF140570, 1 hit SSF46785, SSF46785, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MUKF_ECOLI | |
Accessioni | P60293Primary (citable) accession number: P60293 Secondary accession number(s): P36567 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 16, 2004 |
Last sequence update: | January 16, 2004 | |
Last modified: | December 2, 2020 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families