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Protein

Eukaryotic translation initiation factor 3 subunit E

Gene

Eif3e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.UniRule annotation1 Publication

GO - Molecular functioni

  • protein N-terminus binding Source: MGI
  • translation initiation factor activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit EUniRule annotation
Short name:
eIF3eUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 6UniRule annotation
MMTV integration site 6
Mammary tumor-associated protein INT-6
Viral integration site protein INT-6
eIF-3 p48UniRule annotation
Gene namesi
Name:Eif3e
Synonyms:Eif3s6, Int6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:99257 Eif3e

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

EIF3E serves as a site for viral integration of mouse mammary tumor virus (MMTV) in mammary tumors. MMTV integration into EIF3E can result in EIF3E truncation and expression of chimeric RNA species which terminate at a cryptic transcription stop signal in the reverse U3 portion of the MMTV long terminal repeat. This causes deregulation of the normal control of mammary epithelial cell growth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedUniRule annotation
ChainiPRO_00001235162 – 445Eukaryotic translation initiation factor 3 subunit EAdd BLAST444

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineUniRule annotationBy similarity1
Modified residuei399PhosphoserineBy similarity1
Modified residuei439PhosphothreonineCombined sources1
Modified residuei442PhosphoserineBy similarity1
Modified residuei445PhosphotyrosineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP60229
PaxDbiP60229
PeptideAtlasiP60229
PRIDEiP60229

PTM databases

iPTMnetiP60229
PhosphoSitePlusiP60229
SwissPalmiP60229

Expressioni

Tissue specificityi

Ubiquitously expressed.

Gene expression databases

BgeeiENSMUSG00000022336 Expressed in 277 organ(s), highest expression level in epithelium of cochlear duct
ExpressionAtlasiP60229 baseline and differential
GenevisibleiP60229 MM

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with COPS3, COPS6, COPS7 (COPS7A or COPS7B), EIF4G1, EPAS1, MCM7, NCBP1, PSMC6, TRIM27 and UPF2 (By similarity). Interacts with IFIT1 and IFIT2 (By similarity).UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200775, 115 interactors
DIPiDIP-44570N
IntActiP60229, 117 interactors
MINTiP60229
STRINGi10090.ENSMUSP00000022960

Structurei

3D structure databases

ProteinModelPortaliP60229
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini221 – 398PCIPROSITE-ProRule annotationAdd BLAST178

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni4 – 128Sufficient for interaction with EPAS1UniRule annotationAdd BLAST125
Regioni9 – 195Sufficient for interaction with TRIM27UniRule annotationAdd BLAST187
Regioni351 – 445Sufficient for interaction with MCM7UniRule annotationAdd BLAST95

Sequence similaritiesi

Belongs to the eIF-3 subunit E family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2758 Eukaryota
ENOG410XQK5 LUCA
GeneTreeiENSGT00390000002661
HOGENOMiHOG000036697
HOVERGENiHBG001097
InParanoidiP60229
KOiK03250
OMAiHRTWLIH
OrthoDBiEOG091G075K
PhylomeDBiP60229
TreeFamiTF101518

Family and domain databases

Gene3Di1.10.10.10, 1 hit
HAMAPiMF_03004 eIF3e, 1 hit
InterProiView protein in InterPro
IPR016650 eIF3e
IPR019010 eIF3e_N
IPR000717 PCI_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10317 PTHR10317, 1 hit
PfamiView protein in Pfam
PF09440 eIF3_N, 1 hit
PF01399 PCI, 1 hit
PIRSFiPIRSF016255 eIF3e_su6, 1 hit
SMARTiView protein in SMART
SM01186 eIF3_N, 1 hit
SM00088 PINT, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60229-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAEYDLTTRI AHFLDRHLVF PLLEFLSVKE IYNEKELLQG KLDLLSDTNM
60 70 80 90 100
VDFAMDVYKN LYSDDIPHAL REKRTTVVAQ LKQLQAETEP IVKMFEDPET
110 120 130 140 150
TRQMQSTRDG RMLFDYLADK HGFRQEYLDT LYRYAKFQYE CGNYSGAAEY
160 170 180 190 200
LYFFRVLVPA TDRNALSSLW GKLASEILMQ NWDAAMEDLT RLKETIDNNS
210 220 230 240 250
VSSPLQSLQQ RTWLIHWSLF VFFNHPKGRD NIIDLFLYQP QYLNAIQTMC
260 270 280 290 300
PHILRYLTTA VITNKDVRKR RQVLKDLVKV IQQESYTYKD PITEFVECLY
310 320 330 340 350
VNFDFDGAQK KLRECESVLV NDFFLVACLE DFIENARLFI FETFCRIHQC
360 370 380 390 400
ISINMLADKL NMTPEEAERW IVNLIRNARL DAKIDSKLGH VVMGNNAVSP
410 420 430 440
YQQVIEKTKS LSFRSQMLAM NIEKKLNQNS RSEAPNWATQ DSGFY
Length:445
Mass (Da):52,221
Last modified:January 16, 2004 - v1
Checksum:iA5368651DD0DDD0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S75221 Genomic DNA Translation: AAC00046.1
S75223 Genomic DNA Translation: AAC00047.1
BC029177 mRNA Translation: AAH29177.1
CCDSiCCDS27452.1
RefSeqiNP_032414.1, NM_008388.2
UniGeneiMm.289992

Genome annotation databases

EnsembliENSMUST00000022960; ENSMUSP00000022960; ENSMUSG00000022336
GeneIDi16341
KEGGimmu:16341
UCSCiuc007vpj.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S75221 Genomic DNA Translation: AAC00046.1
S75223 Genomic DNA Translation: AAC00047.1
BC029177 mRNA Translation: AAH29177.1
CCDSiCCDS27452.1
RefSeqiNP_032414.1, NM_008388.2
UniGeneiMm.289992

3D structure databases

ProteinModelPortaliP60229
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200775, 115 interactors
DIPiDIP-44570N
IntActiP60229, 117 interactors
MINTiP60229
STRINGi10090.ENSMUSP00000022960

PTM databases

iPTMnetiP60229
PhosphoSitePlusiP60229
SwissPalmiP60229

Proteomic databases

EPDiP60229
PaxDbiP60229
PeptideAtlasiP60229
PRIDEiP60229

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022960; ENSMUSP00000022960; ENSMUSG00000022336
GeneIDi16341
KEGGimmu:16341
UCSCiuc007vpj.1 mouse

Organism-specific databases

CTDi3646
MGIiMGI:99257 Eif3e

Phylogenomic databases

eggNOGiKOG2758 Eukaryota
ENOG410XQK5 LUCA
GeneTreeiENSGT00390000002661
HOGENOMiHOG000036697
HOVERGENiHBG001097
InParanoidiP60229
KOiK03250
OMAiHRTWLIH
OrthoDBiEOG091G075K
PhylomeDBiP60229
TreeFamiTF101518

Enzyme and pathway databases

ReactomeiR-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition

Miscellaneous databases

ChiTaRSiEif3e mouse
PROiPR:P60229
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022336 Expressed in 277 organ(s), highest expression level in epithelium of cochlear duct
ExpressionAtlasiP60229 baseline and differential
GenevisibleiP60229 MM

Family and domain databases

Gene3Di1.10.10.10, 1 hit
HAMAPiMF_03004 eIF3e, 1 hit
InterProiView protein in InterPro
IPR016650 eIF3e
IPR019010 eIF3e_N
IPR000717 PCI_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10317 PTHR10317, 1 hit
PfamiView protein in Pfam
PF09440 eIF3_N, 1 hit
PF01399 PCI, 1 hit
PIRSFiPIRSF016255 eIF3e_su6, 1 hit
SMARTiView protein in SMART
SM01186 eIF3_N, 1 hit
SM00088 PINT, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEIF3E_MOUSE
AccessioniPrimary (citable) accession number: P60229
Secondary accession number(s): O43902
, Q64058, Q64059, Q64252
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: November 7, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Translation initiation factors
    List of translation initiation factor entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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