UniProtKB - P60033 (CD81_HUMAN)
CD81 antigen
CD81
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 116 | Important for interaction with integrin1 Publication | 1 | |
Sitei | 144 | Important for interaction with integrin1 Publication | 1 | |
Sitei | 148 | Important for interaction with integrin1 Publication | 1 | |
Binding sitei | 219 | CholesterolCombined sources1 Publication | 1 |
GO - Molecular functioni
- cholesterol binding Source: UniProtKB
- integrin binding Source: UniProtKB
- MHC class II protein binding Source: UniProtKB
- MHC class II protein complex binding Source: UniProtKB
- transferrin receptor binding Source: BHF-UCL
- virus receptor activity Source: UniProtKB
GO - Biological processi
- activation of MAPK activity Source: UniProtKB
- CD4-positive, alpha-beta T cell costimulation Source: UniProtKB
- cellular response to low-density lipoprotein particle stimulus Source: UniProtKB
- humoral immune response mediated by circulating immunoglobulin Source: UniProtKB
- immunological synapse formation Source: UniProtKB
- macrophage fusion Source: UniProtKB
- myoblast fusion involved in skeletal muscle regeneration Source: UniProtKB
- osteoclast fusion Source: UniProtKB
- positive regulation of 1-phosphatidylinositol 4-kinase activity Source: UniProtKB
- positive regulation of adaptive immune memory response Source: UniProtKB
- positive regulation of B cell proliferation Source: AgBase
- positive regulation of B cell receptor signaling pathway Source: UniProtKB
- positive regulation of CD4-positive, alpha-beta T cell proliferation Source: UniProtKB
- positive regulation of cell population proliferation Source: UniProtKB
- positive regulation of inflammatory response to antigenic stimulus Source: UniProtKB
- positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
- positive regulation of protein catabolic process in the vacuole Source: BHF-UCL
- positive regulation of protein exit from endoplasmic reticulum Source: UniProtKB
- positive regulation of receptor clustering Source: UniProtKB
- positive regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell Source: UniProtKB
- positive regulation of T cell receptor signaling pathway Source: UniProtKB
- positive regulation of T-helper 2 cell cytokine production Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
- protein localization Source: UniProtKB
- protein localization to lysosome Source: BHF-UCL
- protein localization to plasma membrane Source: UniProtKB
- receptor internalization Source: UniProtKB
- receptor-mediated virion attachment to host cell Source: UniProtKB
- regulation of complement activation Source: Reactome
- regulation of immune response Source: Reactome
- regulation of macrophage migration Source: UniProtKB
- regulation of protein stability Source: BHF-UCL
- viral entry into host cell Source: UniProtKB
Keywordsi
Molecular function | Host cell receptor for virus entry, Receptor |
Biological process | Adaptive immunity, Host-virus interaction, Immunity |
Ligand | Lipid-binding |
Enzyme and pathway databases
PathwayCommonsi | P60033 |
Reactomei | R-HSA-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-HSA-977606, Regulation of Complement cascade |
SignaLinki | P60033 |
Protein family/group databases
TCDBi | 8.A.40.1.1, the tetraspanin (tetraspanin) family |
Names & Taxonomyi
Protein namesi | Recommended name: CD81 antigenAlternative name(s): 26 kDa cell surface protein TAPA-11 Publication Target of the antiproliferative antibody 11 Publication Tetraspanin-28 Short name: Tspan-28 CD_antigen: CD811 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000110651.11 |
HGNCi | HGNC:1701, CD81 |
MIMi | 186845, gene |
neXtProti | NX_P60033 |
Subcellular locationi
Plasma membrane
- Cell membrane 5 Publications; Multi-pass membrane protein Sequence analysis
- Basolateral cell membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Note: Associates with CLDN1 and the CLDN1-CD81 complex localizes to the basolateral cell membrane.1 Publication
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- basal plasma membrane Source: ARUK-UCL
- basolateral plasma membrane Source: UniProtKB
- immunological synapse Source: UniProtKB
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
- tetraspanin-enriched microdomain Source: UniProtKB
Other locations
- focal adhesion Source: UniProtKB
- integral component of membrane Source: UniProtKB
- membrane Source: UniProtKB
- vesicle Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 12 | Cytoplasmic1 Publication1 PublicationAdd BLAST | 12 | |
Transmembranei | 13 – 33 | Helical1 PublicationAdd BLAST | 21 | |
Topological domaini | 34 – 63 | Extracellular1 PublicationAdd BLAST | 30 | |
Transmembranei | 64 – 84 | Helical1 PublicationAdd BLAST | 21 | |
Topological domaini | 85 – 89 | Cytoplasmic1 Publication | 5 | |
Transmembranei | 90 – 112 | Helical1 PublicationAdd BLAST | 23 | |
Topological domaini | 113 – 201 | Extracellular1 PublicationAdd BLAST | 89 | |
Transmembranei | 202 – 224 | Helical1 PublicationAdd BLAST | 23 | |
Topological domaini | 225 – 236 | Cytoplasmic1 Publication1 PublicationAdd BLAST | 12 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Involvement in diseasei
Immunodeficiency, common variable, 6 (CVID6)1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 116 | K → E: Reduces binding to integrin. 1 Publication | 1 | |
Mutagenesisi | 119 | I → A: No effect on integrin binding. 1 Publication | 1 | |
Mutagenesisi | 121 | K → E: No effect on integrin binding. 1 Publication | 1 | |
Mutagenesisi | 124 | K → E: No effect on integrin binding. 1 Publication | 1 | |
Mutagenesisi | 126 | F → A: No effect on integrin binding. 1 Publication | 1 | |
Mutagenesisi | 144 | K → E: Reduces binding to integrin; when associated with E-148. 1 Publication | 1 | |
Mutagenesisi | 148 | K → E: Reduces binding to integrin; when associated with E-144. 1 Publication | 1 | |
Mutagenesisi | 186 | F → A: No effect on integrin binding. 1 Publication | 1 | |
Mutagenesisi | 187 | K → E: No effect on integrin binding. 1 Publication | 1 | |
Mutagenesisi | 188 | E → K or Q: Strongly reduced affinity for HCV protein E2; when associated with E-196. 1 Publication | 1 | |
Mutagenesisi | 188 | E → K: Mildly reduced affinity for HCV protein E2. 1 Publication | 1 | |
Mutagenesisi | 196 | D → E: Strongly reduced affinity for HCV protein E2; when associated with K-188 or Q-188. 1 Publication | 1 | |
Mutagenesisi | 196 | D → K, Q or R: Strongly reduced affinity for HCV protein E2. 1 Publication | 1 | |
Mutagenesisi | 219 | E → A or Q: Reduced affinity for cholesterol binding. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 975 |
MalaCardsi | CD81 |
MIMi | 613496, phenotype |
OpenTargetsi | ENSG00000110651 |
Orphaneti | 1572, Common variable immunodeficiency |
PharmGKBi | PA26240 |
Miscellaneous databases
Pharosi | P60033, Tchem |
Chemistry databases
ChEMBLi | CHEMBL1075180 |
Polymorphism and mutation databases
BioMutai | CD81 |
DMDMi | 38503376 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000219221 | 1 – 236 | CD81 antigenAdd BLAST | 236 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 156 ↔ 190 | Combined sources5 Publications | ||
Disulfide bondi | 157 ↔ 175 | Combined sources5 Publications |
Post-translational modificationi
Keywords - PTMi
Disulfide bondProteomic databases
EPDi | P60033 |
jPOSTi | P60033 |
MassIVEi | P60033 |
MaxQBi | P60033 |
PaxDbi | P60033 |
PeptideAtlasi | P60033 |
PRIDEi | P60033 |
ProteomicsDBi | 57182 |
PTM databases
iPTMneti | P60033 |
PhosphoSitePlusi | P60033 |
SwissPalmi | P60033 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000110651, Expressed in stromal cell of endometrium and 243 other tissues |
ExpressionAtlasi | P60033, baseline and differential |
Genevisiblei | P60033, HS |
Organism-specific databases
HPAi | ENSG00000110651, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer (PubMed:20375010).
Part of a complex composed of CD19, CR2/CD21, CD81 and IFITM1/CD225 in the membrane of mature B cells.
Interacts (via the second extracellular domain) with CD19; this interaction is initiated early during biosynthesis in the ER and enables trafficking of only properly folded CD19 (PubMed:16449649, PubMed:1383329).
Part of a complex that includes MHC class II/HLA-DR molecules and IFITM1 (PubMed:8409388).
Interacts with IFITM1 (PubMed:2398277).
Interacts with IFITM2 and IFITM3 (By similarity). Part of integrin-tetraspanin complex composed of CD9, CD81, beta-1 and beta-2 integrins in the membrane of monocyte/macrophages (PubMed:12796480).
Interacts (via the second extracellular domain) with integrin ITGAV:ITGB3 (PubMed:27993971).
Interacts with CD247/CD3 zeta, ICAM1 and CD9 at the immune synapse on T cell membrane (PubMed:23858057).
Part of a GPCR-tetraspanin complex consisting at least of ADGRG1, CD81, possibly CD9, and GNA11 in which CD81 enhances the association of ADGRG1 with GNA11 (PubMed:15004227).
Part of a complex composed of CD9, CD81, PTGFRN and IGSF8 (By similarity).
Interacts directly with IGSF8 (PubMed:11504738).
Interacts with CD53 and SCIMP (PubMed:21930792).
Interacts with SAMHD1 (via its C-terminus) (PubMed:28871089).
Interacts with glypican GPC3 and with the transcriptional repressor HHEX; binding to GPC3 decreases the availability of free CD81 for binding to HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (By similarity).
Interacts with CLDN1 (PubMed:20375010, PubMed:21516087).
Interacts with CLDN6 and CLDN9 (PubMed:20375010).
By similarity8 Publications(Microbial infection) Plays a critical role in HCV attachment and/or cell entry by interacting with HCV E1/E2 glycoproteins heterodimer.
3 PublicationsBinary interactionsi
Hide detailsP60033
GO - Molecular functioni
- integrin binding Source: UniProtKB
- MHC class II protein binding Source: UniProtKB
- transferrin receptor binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 107413, 267 interactors |
CORUMi | P60033 |
IntActi | P60033, 111 interactors |
MINTi | P60033 |
STRINGi | 9606.ENSP00000263645 |
Chemistry databases
BindingDBi | P60033 |
Miscellaneous databases
RNActi | P60033, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | P60033 |
SMRi | P60033 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P60033 |
Family & Domainsi
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3882, Eukaryota |
GeneTreei | ENSGT00940000158805 |
HOGENOMi | CLU_055524_10_0_1 |
InParanoidi | P60033 |
OrthoDBi | 1205716at2759 |
PhylomeDBi | P60033 |
TreeFami | TF352895 |
Family and domain databases
Gene3Di | 1.10.1450.10, 1 hit |
InterProi | View protein in InterPro IPR000301, Tetraspanin IPR018499, Tetraspanin/Peripherin IPR018503, Tetraspanin_CS IPR008952, Tetraspanin_EC2_sf |
Pfami | View protein in Pfam PF00335, Tetraspanin, 1 hit |
PIRSFi | PIRSF002419, Tetraspanin, 1 hit |
PRINTSi | PR00259, TMFOUR |
SUPFAMi | SSF48652, SSF48652, 1 hit |
PROSITEi | View protein in PROSITE PS00421, TM4_1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 10 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MGVEGCTKCI KYLLFVFNFV FWLAGGVILG VALWLRHDPQ TTNLLYLELG
60 70 80 90 100
DKPAPNTFYV GIYILIAVGA VMMFVGFLGC YGAIQESQCL LGTFFTCLVI
110 120 130 140 150
LFACEVAAGI WGFVNKDQIA KDVKQFYDQA LQQAVVDDDA NNAKAVVKTF
160 170 180 190 200
HETLDCCGSS TLTALTTSVL KNNLCPSGSN IISNLFKEDC HQKIDDLFSG
210 220 230
KLYLIGIAAI VVAVIMIFEM ILSMVLCCGI RNSSVY
Computationally mapped potential isoform sequencesi
There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA6NMH8 | A6NMH8_HUMAN | Tetraspanin | CD81 | 274 | Annotation score: | ||
E9PJK1 | E9PJK1_HUMAN | Tetraspanin | CD81 | 165 | Annotation score: | ||
E9PRJ8 | E9PRJ8_HUMAN | Tetraspanin | CD81 | 209 | Annotation score: | ||
E9PIF1 | E9PIF1_HUMAN | Tetraspanin | CD81 | 242 | Annotation score: | ||
H0YEE2 | H0YEE2_HUMAN | Tetraspanin | CD81 | 148 | Annotation score: | ||
H0YDJ9 | H0YDJ9_HUMAN | Tetraspanin | CD81 | 225 | Annotation score: | ||
H0YDL9 | H0YDL9_HUMAN | Tetraspanin | CD81 | 221 | Annotation score: | ||
E9PQV4 | E9PQV4_HUMAN | CD81 antigen | CD81 | 99 | Annotation score: | ||
E9PM31 | E9PM31_HUMAN | CD81 antigen | CD81 | 92 | Annotation score: | ||
E9PPF5 | E9PPF5_HUMAN | CD81 antigen | CD81 | 51 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M33680 mRNA Translation: AAA36663.1 BT019507 mRNA Translation: AAV38314.1 BT019508 mRNA Translation: AAV38315.1 EF064749 Genomic DNA Translation: ABK41932.1 AC129929 Genomic DNA No translation available. AC124057 Genomic DNA No translation available. BC002978 mRNA Translation: AAH02978.1 BC093047 mRNA Translation: AAH93047.1 |
CCDSi | CCDS7734.1 |
PIRi | A35649 |
RefSeqi | NP_001284578.1, NM_001297649.1 NP_004347.1, NM_004356.3 |
Genome annotation databases
Ensembli | ENST00000263645; ENSP00000263645; ENSG00000110651 |
GeneIDi | 975 |
KEGGi | hsa:975 |
UCSCi | uc001lwf.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M33680 mRNA Translation: AAA36663.1 BT019507 mRNA Translation: AAV38314.1 BT019508 mRNA Translation: AAV38315.1 EF064749 Genomic DNA Translation: ABK41932.1 AC129929 Genomic DNA No translation available. AC124057 Genomic DNA No translation available. BC002978 mRNA Translation: AAH02978.1 BC093047 mRNA Translation: AAH93047.1 |
CCDSi | CCDS7734.1 |
PIRi | A35649 |
RefSeqi | NP_001284578.1, NM_001297649.1 NP_004347.1, NM_004356.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1G8Q | X-ray | 1.60 | A/B | 113-201 | [»] | |
1IV5 | X-ray | 2.60 | A/B | 113-201 | [»] | |
2AVZ | model | - | A | 1-236 | [»] | |
3X0E | X-ray | 1.84 | A/B | 113-202 | [»] | |
5DFV | X-ray | 2.80 | A/B | 112-201 | [»] | |
5DFW | X-ray | 2.33 | A | 112-201 | [»] | |
5M2C | X-ray | 1.96 | A/B | 112-201 | [»] | |
5M33 | X-ray | 1.28 | A/B | 113-201 | [»] | |
5M3D | X-ray | 2.38 | A/B/C/D | 112-201 | [»] | |
5M3T | X-ray | 2.02 | A/B | 112-201 | [»] | |
5M4R | X-ray | 3.10 | A/B/C/D/E | 112-201 | [»] | |
5TCX | X-ray | 2.96 | A | 2-236 | [»] | |
6EJG | X-ray | 2.82 | A/B | 112-202 | [»] | |
6EJM | X-ray | 2.15 | A/B | 112-202 | [»] | |
6EK2 | X-ray | 2.65 | A/B | 112-201 | [»] | |
6U9S | X-ray | 2.40 | C/F | 112-200 | [»] | |
BMRBi | P60033 | |||||
SMRi | P60033 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107413, 267 interactors |
CORUMi | P60033 |
IntActi | P60033, 111 interactors |
MINTi | P60033 |
STRINGi | 9606.ENSP00000263645 |
Chemistry databases
BindingDBi | P60033 |
ChEMBLi | CHEMBL1075180 |
Protein family/group databases
TCDBi | 8.A.40.1.1, the tetraspanin (tetraspanin) family |
PTM databases
iPTMneti | P60033 |
PhosphoSitePlusi | P60033 |
SwissPalmi | P60033 |
Polymorphism and mutation databases
BioMutai | CD81 |
DMDMi | 38503376 |
Proteomic databases
EPDi | P60033 |
jPOSTi | P60033 |
MassIVEi | P60033 |
MaxQBi | P60033 |
PaxDbi | P60033 |
PeptideAtlasi | P60033 |
PRIDEi | P60033 |
ProteomicsDBi | 57182 |
Protocols and materials databases
ABCDi | P60033, 10 sequenced antibodies |
Antibodypediai | 1570, 1253 antibodies |
DNASUi | 975 |
Genome annotation databases
Ensembli | ENST00000263645; ENSP00000263645; ENSG00000110651 |
GeneIDi | 975 |
KEGGi | hsa:975 |
UCSCi | uc001lwf.2, human |
Organism-specific databases
CTDi | 975 |
DisGeNETi | 975 |
EuPathDBi | HostDB:ENSG00000110651.11 |
GeneCardsi | CD81 |
HGNCi | HGNC:1701, CD81 |
HPAi | ENSG00000110651, Low tissue specificity |
MalaCardsi | CD81 |
MIMi | 186845, gene 613496, phenotype |
neXtProti | NX_P60033 |
OpenTargetsi | ENSG00000110651 |
Orphaneti | 1572, Common variable immunodeficiency |
PharmGKBi | PA26240 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3882, Eukaryota |
GeneTreei | ENSGT00940000158805 |
HOGENOMi | CLU_055524_10_0_1 |
InParanoidi | P60033 |
OrthoDBi | 1205716at2759 |
PhylomeDBi | P60033 |
TreeFami | TF352895 |
Enzyme and pathway databases
PathwayCommonsi | P60033 |
Reactomei | R-HSA-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-HSA-977606, Regulation of Complement cascade |
SignaLinki | P60033 |
Miscellaneous databases
BioGRID-ORCSi | 975, 11 hits in 845 CRISPR screens |
ChiTaRSi | CD81, human |
EvolutionaryTracei | P60033 |
GeneWikii | CD81 |
GenomeRNAii | 975 |
Pharosi | P60033, Tchem |
PROi | PR:P60033 |
RNActi | P60033, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000110651, Expressed in stromal cell of endometrium and 243 other tissues |
ExpressionAtlasi | P60033, baseline and differential |
Genevisiblei | P60033, HS |
Family and domain databases
Gene3Di | 1.10.1450.10, 1 hit |
InterProi | View protein in InterPro IPR000301, Tetraspanin IPR018499, Tetraspanin/Peripherin IPR018503, Tetraspanin_CS IPR008952, Tetraspanin_EC2_sf |
Pfami | View protein in Pfam PF00335, Tetraspanin, 1 hit |
PIRSFi | PIRSF002419, Tetraspanin, 1 hit |
PRINTSi | PR00259, TMFOUR |
SUPFAMi | SSF48652, SSF48652, 1 hit |
PROSITEi | View protein in PROSITE PS00421, TM4_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CD81_HUMAN | |
Accessioni | P60033Primary (citable) accession number: P60033 Secondary accession number(s): P18582, Q5U0J6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 21, 2003 |
Last sequence update: | November 21, 2003 | |
Last modified: | December 2, 2020 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human cell differentiation molecules
CD nomenclature of surface proteins of human leucocytes and list of entries