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Protein

CD81 antigen

Gene

CD81

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV.
(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Associates with CLDN1 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei116Important for interaction with integrin1 Publication1
Sitei144Important for interaction with integrin1 Publication1
Sitei148Important for interaction with integrin1 Publication1

GO - Molecular functioni

  • integrin binding Source: UniProtKB
  • MHC class II protein complex binding Source: UniProtKB
  • transferrin receptor binding Source: BHF-UCL
  • virus receptor activity Source: UniProtKB

GO - Biological processi

  • activation of MAPK activity Source: UniProtKB
  • cell proliferation Source: ProtInc
  • cell surface receptor signaling pathway Source: GO_Central
  • cellular response to low-density lipoprotein particle stimulus Source: UniProtKB
  • positive regulation of 1-phosphatidylinositol 4-kinase activity Source: UniProtKB
  • positive regulation of B cell proliferation Source: AgBase
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
  • positive regulation of protein catabolic process in the vacuole Source: BHF-UCL
  • positive regulation of transcription by RNA polymerase II Source: BHF-UCL
  • protein localization Source: UniProtKB
  • protein localization to lysosome Source: BHF-UCL
  • receptor internalization Source: UniProtKB
  • receptor-mediated virion attachment to host cell Source: UniProtKB
  • regulation of cell motility Source: Ensembl
  • regulation of complement activation Source: Reactome
  • regulation of immune response Source: Reactome
  • regulation of protein stability Source: BHF-UCL
  • viral entry into host cell Source: UniProtKB

Keywordsi

Molecular functionHost cell receptor for virus entry, Receptor
Biological processHost-virus interaction

Enzyme and pathway databases

ReactomeiR-HSA-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-HSA-977606 Regulation of Complement cascade
SignaLinkiP60033

Protein family/group databases

TCDBi8.A.40.1.1 the tetraspanin (tetraspanin) family

Names & Taxonomyi

Protein namesi
Recommended name:
CD81 antigen
Alternative name(s):
26 kDa cell surface protein TAPA-1
Target of the antiproliferative antibody 1
Tetraspanin-28
Short name:
Tspan-28
CD_antigen: CD81
Gene namesi
Name:CD81
Synonyms:TAPA1, TSPAN28
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000110651.11
HGNCiHGNC:1701 CD81
MIMi186845 gene
neXtProtiNX_P60033

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 33HelicalSequence analysisAdd BLAST21
Topological domaini34 – 63ExtracellularSequence analysisAdd BLAST30
Transmembranei64 – 84HelicalSequence analysisAdd BLAST21
Topological domaini85 – 89CytoplasmicSequence analysis5
Transmembranei90 – 112HelicalSequence analysisAdd BLAST23
Topological domaini113 – 201ExtracellularSequence analysisAdd BLAST89
Transmembranei202 – 224HelicalSequence analysisAdd BLAST23
Topological domaini225 – 236CytoplasmicSequence analysisAdd BLAST12

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Involvement in diseasei

Immunodeficiency, common variable, 6 (CVID6)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.
See also OMIM:613496

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi116K → E: Reduces binding to integrin. 1 Publication1
Mutagenesisi119I → A: No effect on integrin binding. 1 Publication1
Mutagenesisi121K → E: No effect on integrin binding. 1 Publication1
Mutagenesisi124K → E: No effect on integrin binding. 1 Publication1
Mutagenesisi126F → A: No effect on integrin binding. 1 Publication1
Mutagenesisi144K → E: Reduces binding to integrin; when associated with E-148. 1 Publication1
Mutagenesisi148K → E: Reduces binding to integrin; when associated with E-144. 1 Publication1
Mutagenesisi186F → A: No effect on integrin binding. 1 Publication1
Mutagenesisi187K → E: No effect on integrin binding. 1 Publication1

Organism-specific databases

DisGeNETi975
MalaCardsiCD81
MIMi613496 phenotype
OpenTargetsiENSG00000110651
Orphaneti1572 Common variable immunodeficiency
PharmGKBiPA26240

Chemistry databases

ChEMBLiCHEMBL1075180

Polymorphism and mutation databases

BioMutaiCD81
DMDMi38503376

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002192211 – 236CD81 antigenAdd BLAST236

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi156 ↔ 190
Disulfide bondi157 ↔ 175

Post-translational modificationi

Not glycosylated.Curated

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP60033
MaxQBiP60033
PaxDbiP60033
PeptideAtlasiP60033
PRIDEiP60033
ProteomicsDBi57182

PTM databases

iPTMnetiP60033
PhosphoSitePlusiP60033
SwissPalmiP60033

Expressioni

Tissue specificityi

Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.

Gene expression databases

BgeeiENSG00000110651
CleanExiHS_CD81
ExpressionAtlasiP60033 baseline and differential
GenevisibleiP60033 HS

Organism-specific databases

HPAiCAB002507
HPA007234

Interactioni

Subunit structurei

Homodimer (PubMed:20375010). Interacts directly with IGSF8 (PubMed:11504738). Interacts with CD53 and SCIMP (PubMed:21930792). Interacts with IFITM1 (PubMed:2398277). Interacts with IFITM2 and IFITM3 (By similarity). Part of a GPCR-tetraspanin complex consisting at least of ADGRG1, CD81, eventually CD9, and GNA11 in which CD81 enhances the association of ADGRG1 with GNA11 (PubMed:15004227). Interacts with CLDN1 (PubMed:20375010, PubMed:21516087). Interacts with CLDN6 and CLDN9 (PubMed:20375010). Interacts (via the second extracellular domain) with integrin ITGAV:ITGB3 (PubMed:27993971).By similarity7 Publications
(Microbial infection) Plays a critical role in HCV attachment and/or cell entry by interacting with HCV E1/E2 glycoproteins heterodimer.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • integrin binding Source: UniProtKB
  • transferrin receptor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi107413, 201 interactors
CORUMiP60033
IntActiP60033, 61 interactors
MINTiP60033
STRINGi9606.ENSP00000263645

Chemistry databases

BindingDBiP60033

Structurei

Secondary structure

1236
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 36Combined sources27
Helixi57 – 80Combined sources24
Helixi81 – 84Combined sources4
Helixi89 – 114Combined sources26
Helixi116 – 136Combined sources21
Helixi141 – 154Combined sources14
Beta strandi158 – 160Combined sources3
Helixi163 – 165Combined sources3
Helixi166 – 171Combined sources6
Helixi172 – 174Combined sources3
Helixi181 – 185Combined sources5
Helixi190 – 199Combined sources10
Helixi202 – 230Combined sources29

3D structure databases

ProteinModelPortaliP60033
SMRiP60033
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60033

Family & Domainsi

Sequence similaritiesi

Belongs to the tetraspanin (TM4SF) family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3882 Eukaryota
ENOG4111IRY LUCA
GeneTreeiENSGT00880000137858
HOGENOMiHOG000230651
HOVERGENiHBG002324
InParanoidiP60033
KOiK06508
PhylomeDBiP60033
TreeFamiTF352895

Family and domain databases

Gene3Di1.10.1450.10, 1 hit
InterProiView protein in InterPro
IPR000301 Tetraspanin
IPR018499 Tetraspanin/Peripherin
IPR018503 Tetraspanin_CS
IPR008952 Tetraspanin_EC2_sf
PfamiView protein in Pfam
PF00335 Tetraspannin, 1 hit
PIRSFiPIRSF002419 Tetraspanin, 1 hit
PRINTSiPR00259 TMFOUR
SUPFAMiSSF48652 SSF48652, 1 hit
PROSITEiView protein in PROSITE
PS00421 TM4_1, 1 hit

Sequencei

Sequence statusi: Complete.

P60033-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVEGCTKCI KYLLFVFNFV FWLAGGVILG VALWLRHDPQ TTNLLYLELG
60 70 80 90 100
DKPAPNTFYV GIYILIAVGA VMMFVGFLGC YGAIQESQCL LGTFFTCLVI
110 120 130 140 150
LFACEVAAGI WGFVNKDQIA KDVKQFYDQA LQQAVVDDDA NNAKAVVKTF
160 170 180 190 200
HETLDCCGSS TLTALTTSVL KNNLCPSGSN IISNLFKEDC HQKIDDLFSG
210 220 230
KLYLIGIAAI VVAVIMIFEM ILSMVLCCGI RNSSVY
Length:236
Mass (Da):25,809
Last modified:November 21, 2003 - v1
Checksum:iEB9BD7671AC91B4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33680 mRNA Translation: AAA36663.1
BT019507 mRNA Translation: AAV38314.1
BT019508 mRNA Translation: AAV38315.1
EF064749 Genomic DNA Translation: ABK41932.1
AC129929 Genomic DNA No translation available.
AC124057 Genomic DNA No translation available.
BC002978 mRNA Translation: AAH02978.1
BC093047 mRNA Translation: AAH93047.1
CCDSiCCDS7734.1
PIRiA35649
RefSeqiNP_001284578.1, NM_001297649.1
NP_004347.1, NM_004356.3
UniGeneiHs.54457

Genome annotation databases

EnsembliENST00000263645; ENSP00000263645; ENSG00000110651
GeneIDi975
KEGGihsa:975
UCSCiuc001lwf.2 human

Similar proteinsi

Entry informationi

Entry nameiCD81_HUMAN
AccessioniPrimary (citable) accession number: P60033
Secondary accession number(s): P18582, Q5U0J6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: November 21, 2003
Last modified: July 18, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

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