UniProtKB - P60010 (ACT_YEAST)
Actin
ACT1
Functioni
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
GO - Molecular functioni
- ATP binding Source: WormBase
- identical protein binding Source: IntAct
- structural constituent of cytoskeleton Source: SGD
GO - Biological processi
- ascospore wall assembly Source: SGD
- cellular response to oxidative stress Source: SGD
- chromatin remodeling Source: ComplexPortal
- DNA repair Source: SGD
- endocytosis Source: SGD
- establishment of cell polarity Source: SGD
- histone acetylation Source: SGD
- mitotic actomyosin contractile ring contraction Source: SGD
- protein secretion Source: SGD
- vacuole inheritance Source: SGD
Keywordsi
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-114608, Platelet degranulation R-SCE-196025, Formation of annular gap junctions R-SCE-2029482, Regulation of actin dynamics for phagocytic cup formation R-SCE-5626467, RHO GTPases activate IQGAPs R-SCE-5663213, RHO GTPases Activate WASPs and WAVEs |
Protein family/group databases
MoonDBi | P60010, Predicted |
Names & Taxonomyi
Protein namesi | Recommended name: Actin |
Gene namesi | Name:ACT1 Synonyms:ABY1, END7 Ordered Locus Names:YFL039C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001855, ACT1 |
VEuPathDBi | FungiDB:YFL039C |
Subcellular locationi
Cytoskeleton
Cytoskeleton
- actin cortical patch Source: SGD
- actin filament Source: SGD
- actin filament bundle Source: SGD
- cellular bud neck contractile ring Source: SGD
Nucleus
- Ino80 complex Source: SGD
- NuA4 histone acetyltransferase complex Source: SGD
- nucleus Source: ComplexPortal
- Swr1 complex Source: SGD
Other locations
- chromatin Source: ComplexPortal
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 183 | R → K: No effect on growth kinetics nor on actin cytoskeleton morphology. Resistant to Chivosazole F inhibition of polymerization. Increases penetrance of Latrunculin A inhibition of polymerization. No effect on Chondramide inhibition of polymerization. 1 Publication | 1 | |
Mutagenesisi | 335 | R → K: No effect on growth kinetics nor on actin cytoskeleton morphology. Resistant to Chivosazole F and Latrunculin A inhibition of polymerization. No effect on Chondramide inhibition of polymerization. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000089051 | 1 – 375 | ActinAdd BLAST | 375 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources1 Publication | 1 | |
Cross-linki | 191 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources |
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 73 | Not methylated1 Publication | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Ubl conjugationProteomic databases
MaxQBi | P60010 |
PaxDbi | P60010 |
PRIDEi | P60010 |
2D gel databases
SWISS-2DPAGEi | P60010 |
PTM databases
CarbonylDBi | P60010 |
iPTMneti | P60010 |
Interactioni
Subunit structurei
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix (Probable). Treatments with Lantrunculin A, the microbial peptide Chondramide or the microbial metabolite Chivazole F inhibit actin polymerization (PubMed:28796488). Each actin can bind to 4 others.
Interacts with YIH1 (via C-terminus); this interaction occurs in a GCN1-independent manner (PubMed:15126500, PubMed:21239490).
Component of the INO80 complex.
Component of the SWR1 complex.
Component of the NuA4 complex.
Curated3 PublicationsBinary interactionsi
P60010
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 31107, 2325 interactors |
ComplexPortali | CPX-2122, Swr1 chromatin remodelling complex CPX-3155, NuA4 histone acetyltransferase complex CPX-863, INO80 chromatin remodeling complex |
DIPi | DIP-310N |
IntActi | P60010, 238 interactors |
MINTi | P60010 |
STRINGi | 4932.YFL039C |
Chemistry databases
BindingDBi | P60010 |
Miscellaneous databases
RNActi | P60010, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P60010 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P60010 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0676, Eukaryota |
GeneTreei | ENSGT00950000182960 |
HOGENOMi | CLU_027965_0_2_1 |
InParanoidi | P60010 |
OMAi | FHTTAER |
Family and domain databases
InterProi | View protein in InterPro IPR004000, Actin IPR020902, Actin/actin-like_CS IPR004001, Actin_CS IPR043129, ATPase_NBD |
PANTHERi | PTHR11937, PTHR11937, 1 hit |
Pfami | View protein in Pfam PF00022, Actin, 1 hit |
PRINTSi | PR00190, ACTIN |
SMARTi | View protein in SMART SM00268, ACTIN, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
PROSITEi | View protein in PROSITE PS00406, ACTINS_1, 1 hit PS00432, ACTINS_2, 1 hit PS01132, ACTINS_ACT_LIKE, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MDSEVAALVI DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGIMVGMGQK
60 70 80 90 100
DSYVGDEAQS KRGILTLRYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE
110 120 130 140 150
HPVLLTEAPM NPKSNREKMT QIMFETFNVP AFYVSIQAVL SLYSSGRTTG
160 170 180 190 200
IVLDSGDGVT HVVPIYAGFS LPHAILRIDL AGRDLTDYLM KILSERGYSF
210 220 230 240 250
STTAEREIVR DIKEKLCYVA LDFEQEMQTA AQSSSIEKSY ELPDGQVITI
260 270 280 290 300
GNERFRAPEA LFHPSVLGLE SAGIDQTTYN SIMKCDVDVR KELYGNIVMS
310 320 330 340 350
GGTTMFPGIA ERMQKEITAL APSSMKVKII APPERKYSVW IGGSILASLT
360 370
TFQQMWISKQ EYDESGPSIV HHKCF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 178 | I → L in CAA24598 (PubMed:7001447).Curated | 1 | |
Sequence conflicti | 308 | G → S in CAA24598 (PubMed:7001447).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 143 | Y → F in strain: CBS 1907. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01288 Genomic DNA Translation: CAA24597.1 V01289 Genomic DNA Translation: CAA24598.1 Sequence problems. V01290 Genomic DNA Translation: CAA24599.1 L00026 Genomic DNA Translation: AAA34391.1 D50617 Genomic DNA Translation: BAA21512.1 AJ389075 Genomic DNA Translation: CAC00716.1 AJ389076 Genomic DNA Translation: CAC00717.1 BK006940 Genomic DNA Translation: DAA12401.1 |
PIRi | A03005, ATBY JS0702 |
RefSeqi | NP_116614.1, NM_001179927.1 |
Genome annotation databases
EnsemblFungii | YFL039C_mRNA; YFL039C; YFL039C |
GeneIDi | 850504 |
KEGGi | sce:YFL039C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01288 Genomic DNA Translation: CAA24597.1 V01289 Genomic DNA Translation: CAA24598.1 Sequence problems. V01290 Genomic DNA Translation: CAA24599.1 L00026 Genomic DNA Translation: AAA34391.1 D50617 Genomic DNA Translation: BAA21512.1 AJ389075 Genomic DNA Translation: CAC00716.1 AJ389076 Genomic DNA Translation: CAC00717.1 BK006940 Genomic DNA Translation: DAA12401.1 |
PIRi | A03005, ATBY JS0702 |
RefSeqi | NP_116614.1, NM_001179927.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1YAG | X-ray | 1.90 | A | 1-375 | [»] | |
1YVN | X-ray | 2.10 | A | 1-375 | [»] | |
5NBL | X-ray | 2.80 | C/D | 1-375 | [»] | |
5NBM | X-ray | 3.40 | C/D | 1-375 | [»] | |
5NBN | X-ray | 4.00 | C/D | 1-375 | [»] | |
5Y81 | electron microscopy | 4.70 | G | 1-375 | [»] | |
SMRi | P60010 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31107, 2325 interactors |
ComplexPortali | CPX-2122, Swr1 chromatin remodelling complex CPX-3155, NuA4 histone acetyltransferase complex CPX-863, INO80 chromatin remodeling complex |
DIPi | DIP-310N |
IntActi | P60010, 238 interactors |
MINTi | P60010 |
STRINGi | 4932.YFL039C |
Chemistry databases
BindingDBi | P60010 |
Protein family/group databases
MoonDBi | P60010, Predicted |
PTM databases
CarbonylDBi | P60010 |
iPTMneti | P60010 |
2D gel databases
SWISS-2DPAGEi | P60010 |
Proteomic databases
MaxQBi | P60010 |
PaxDbi | P60010 |
PRIDEi | P60010 |
Protocols and materials databases
ABCDi | P60010, 1 sequenced antibody |
Genome annotation databases
EnsemblFungii | YFL039C_mRNA; YFL039C; YFL039C |
GeneIDi | 850504 |
KEGGi | sce:YFL039C |
Organism-specific databases
SGDi | S000001855, ACT1 |
VEuPathDBi | FungiDB:YFL039C |
Phylogenomic databases
eggNOGi | KOG0676, Eukaryota |
GeneTreei | ENSGT00950000182960 |
HOGENOMi | CLU_027965_0_2_1 |
InParanoidi | P60010 |
OMAi | FHTTAER |
Enzyme and pathway databases
Reactomei | R-SCE-114608, Platelet degranulation R-SCE-196025, Formation of annular gap junctions R-SCE-2029482, Regulation of actin dynamics for phagocytic cup formation R-SCE-5626467, RHO GTPases activate IQGAPs R-SCE-5663213, RHO GTPases Activate WASPs and WAVEs |
Miscellaneous databases
EvolutionaryTracei | P60010 |
PROi | PR:P60010 |
RNActi | P60010, protein |
Family and domain databases
InterProi | View protein in InterPro IPR004000, Actin IPR020902, Actin/actin-like_CS IPR004001, Actin_CS IPR043129, ATPase_NBD |
PANTHERi | PTHR11937, PTHR11937, 1 hit |
Pfami | View protein in Pfam PF00022, Actin, 1 hit |
PRINTSi | PR00190, ACTIN |
SMARTi | View protein in SMART SM00268, ACTIN, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
PROSITEi | View protein in PROSITE PS00406, ACTINS_1, 1 hit PS00432, ACTINS_2, 1 hit PS01132, ACTINS_ACT_LIKE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACT_YEAST | |
Accessioni | P60010Primary (citable) accession number: P60010 Secondary accession number(s): D6VTJ1 Q9P3X7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | February 23, 2022 | |
This is version 188 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VI
Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families