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Protein

Actin-related protein 2/3 complex subunit 4

Gene

ARPC4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).2 Publications

GO - Molecular functioni

  • actin binding Source: UniProtKB-KW
  • enzyme binding Source: BHF-UCL
  • structural constituent of cytoskeleton Source: FlyBase

GO - Biological processi

Keywordsi

Molecular functionActin-binding

Enzyme and pathway databases

ReactomeiR-HSA-2029482 Regulation of actin dynamics for phagocytic cup formation
R-HSA-3928662 EPHB-mediated forward signaling
R-HSA-5663213 RHO GTPases Activate WASPs and WAVEs
R-HSA-8856828 Clathrin-mediated endocytosis
SIGNORiP59998

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-related protein 2/3 complex subunit 4
Alternative name(s):
Arp2/3 complex 20 kDa subunit
Short name:
p20-ARC
Gene namesi
Name:ARPC4
Synonyms:ARC20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000241553.12
HGNCiHGNC:707 ARPC4
MIMi604226 gene
neXtProtiNX_P59998

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000241553
PharmGKBiPA25002

Chemistry databases

DrugBankiDB08235 N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide

Polymorphism and mutation databases

BioMutaiARPC4
DMDMi38372625

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001240492 – 168Actin-related protein 2/3 complex subunit 4Add BLAST167

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP59998
MaxQBiP59998
PaxDbiP59998
PeptideAtlasiP59998
PRIDEiP59998
ProteomicsDBi57176
TopDownProteomicsiP59998-1 [P59998-1]

PTM databases

iPTMnetiP59998
PhosphoSitePlusiP59998
SwissPalmiP59998

Expressioni

Gene expression databases

BgeeiENSG00000241553 Expressed in 230 organ(s), highest expression level in vagina
CleanExiHS_ARPC4
ExpressionAtlasiP59998 baseline and differential
GenevisibleiP59998 HS

Interactioni

Subunit structurei

Component of the Arp2/3 complex composed of ACTR2/ARP2, ACTR3/ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC.3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115400, 79 interactors
1529294, 12 interactors
CORUMiP59998
DIPiDIP-33188N
IntActiP59998, 41 interactors
MINTiP59998
STRINGi9606.ENSP00000388169

Structurei

3D structure databases

ProteinModelPortaliP59998
SMRiP59998
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ARPC4 family.Curated

Phylogenomic databases

eggNOGiKOG1876 Eukaryota
ENOG4111G2F LUCA
GeneTreeiENSGT00390000016233
ENSGT00760000118951
HOGENOMiHOG000202303
HOVERGENiHBG050582
InParanoidiP59998
KOiK05755
OMAiMLKHKIV
OrthoDBiEOG091G0OI8
PhylomeDBiP59998
TreeFamiTF105621
TF313087

Family and domain databases

Gene3Di3.30.1460.20, 1 hit
InterProiView protein in InterPro
IPR034666 ARPC2/4
IPR008384 ARPC4
PANTHERiPTHR22629 PTHR22629, 1 hit
PfamiView protein in Pfam
PF05856 ARPC4, 1 hit
PIRSFiPIRSF039100 ARPC4, 1 hit
SUPFAMiSSF69645 SSF69645, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P59998-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTATLRPYLS AVRATLQAAL CLENFSSQVV ERHNKPEVEV RSSKELLLQP
60 70 80 90 100
VTISRNEKEK VLIEGSINSV RVSIAVKQAD EIEKILCHKF MRFMMMRAEN
110 120 130 140 150
FFILRRKPVE GYDISFLITN FHTEQMYKHK LVDFVIHFME EIDKEISEMK
160
LSVNARARIV AEEFLKNF
Length:168
Mass (Da):19,667
Last modified:January 23, 2007 - v3
Checksum:i273CCB230AC703DF
GO
Isoform 2 (identifier: P59998-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     110-168: EGYDISFLIT...IVAEEFLKNF → EQKKIFTIQG...LRPCRPQARP

Note: No experimental confirmation available.
Show »
Length:625
Mass (Da):71,719
Checksum:iE05F7DC236DF9674
GO
Isoform 3 (identifier: P59998-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVREPGPRPGTPGCSASGQW

Note: No experimental confirmation available.
Show »
Length:187
Mass (Da):21,588
Checksum:iF61DD748D01B8880
GO
Isoform 4 (identifier: P59998-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-90: Missing.

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:78
Mass (Da):9,552
Checksum:i32DD605A770B6706
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8WDD7F8WDD7_HUMAN
Actin-related protein 2/3 complex s...
ARPC4
113Annotation score:
F8WDW3F8WDW3_HUMAN
Actin-related protein 2/3 complex s...
ARPC4
44Annotation score:
R4GN08R4GN08_HUMAN
Actin-related protein 2/3 complex s...
ARPC4
77Annotation score:

Sequence cautioni

The sequence AAH12596 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94M → T in AAH12596 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0467531 – 90Missing in isoform 4. CuratedAdd BLAST90
Alternative sequenceiVSP_0461501M → MVREPGPRPGTPGCSASGQW in isoform 3. 1 Publication1
Alternative sequenceiVSP_046151110 – 168EGYDI…FLKNF → EQKKIFTIQGCYPVIRCLLR RRGWVEKKMVHRSGPTLLPP QKDLDSSAMGDSDTTEDEDE DEDEEFQPSQLFDFDDLLKF DDLDGTHALMVGLCLNLRNL PWFDEVDANSFFPRCYCLGA EDDKKAFIGDKQPKKQEKNP VLVSPEFVDEALCACEEYLS NLAHMDIDKDLEAPLYLTPE GWSLFLQRYYQVVHEGAELR HLDTQVQRCEDILQQLQAVV PQIDMEGDRNIWIVKPGAKS RGRGIMCMDHLEEMLKLVNG NPVVMKDGKWVVQKYIERPL LIFGTKFDLRQWFLVTDWNP LTVWFYRDSYIRFSTQPFSL KNLDNSVHLCNNSIQKHLEN SCHRHPLLPPDNMWSSQRFQ AHLQEMGAPNAWSTIIVPGM KDAVIHALQTSQDTVQCRKA SFELYGADFVFGEDFQPWLI EINASPTMAPSTAVTARLCA GVQADTLRVVIDRMLDRNCD TGAFELIYKQPVTTSPASTP RPSCLLPMYSDTRARSSDDS TASWWALRPCRPQARP in isoform 2. 1 PublicationAdd BLAST59

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019888 mRNA Translation: AAB71548.1
AF006087 mRNA Translation: AAB64192.1
BX419672 mRNA No translation available.
AC022382 Genomic DNA No translation available.
BC012596 mRNA Translation: AAH12596.3 Different initiation.
BC025289 mRNA No translation available.
CCDSiCCDS43047.1 [P59998-1]
CCDS46743.1 [P59998-4]
CCDS56238.1 [P59998-3]
RefSeqiNP_001020130.1, NM_001024959.2 [P59998-4]
NP_001020131.1, NM_001024960.2 [P59998-4]
NP_001185709.1, NM_001198780.1 [P59998-3]
NP_005709.1, NM_005718.4 [P59998-1]
UniGeneiHs.323342

Genome annotation databases

EnsembliENST00000397261; ENSP00000380431; ENSG00000241553 [P59998-1]
ENST00000433034; ENSP00000388169; ENSG00000241553 [P59998-3]
ENST00000498623; ENSP00000432235; ENSG00000241553 [P59998-4]
GeneIDi10093
KEGGihsa:10093
UCSCiuc003bsz.3 human [P59998-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019888 mRNA Translation: AAB71548.1
AF006087 mRNA Translation: AAB64192.1
BX419672 mRNA No translation available.
AC022382 Genomic DNA No translation available.
BC012596 mRNA Translation: AAH12596.3 Different initiation.
BC025289 mRNA No translation available.
CCDSiCCDS43047.1 [P59998-1]
CCDS46743.1 [P59998-4]
CCDS56238.1 [P59998-3]
RefSeqiNP_001020130.1, NM_001024959.2 [P59998-4]
NP_001020131.1, NM_001024960.2 [P59998-4]
NP_001185709.1, NM_001198780.1 [P59998-3]
NP_005709.1, NM_005718.4 [P59998-1]
UniGeneiHs.323342

3D structure databases

ProteinModelPortaliP59998
SMRiP59998
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115400, 79 interactors
1529294, 12 interactors
CORUMiP59998
DIPiDIP-33188N
IntActiP59998, 41 interactors
MINTiP59998
STRINGi9606.ENSP00000388169

Chemistry databases

DrugBankiDB08235 N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide

PTM databases

iPTMnetiP59998
PhosphoSitePlusiP59998
SwissPalmiP59998

Polymorphism and mutation databases

BioMutaiARPC4
DMDMi38372625

Proteomic databases

EPDiP59998
MaxQBiP59998
PaxDbiP59998
PeptideAtlasiP59998
PRIDEiP59998
ProteomicsDBi57176
TopDownProteomicsiP59998-1 [P59998-1]

Protocols and materials databases

DNASUi10093
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000397261; ENSP00000380431; ENSG00000241553 [P59998-1]
ENST00000433034; ENSP00000388169; ENSG00000241553 [P59998-3]
ENST00000498623; ENSP00000432235; ENSG00000241553 [P59998-4]
GeneIDi10093
KEGGihsa:10093
UCSCiuc003bsz.3 human [P59998-1]

Organism-specific databases

CTDi10093
EuPathDBiHostDB:ENSG00000241553.12
GeneCardsiARPC4
HGNCiHGNC:707 ARPC4
MIMi604226 gene
neXtProtiNX_P59998
OpenTargetsiENSG00000241553
PharmGKBiPA25002
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1876 Eukaryota
ENOG4111G2F LUCA
GeneTreeiENSGT00390000016233
ENSGT00760000118951
HOGENOMiHOG000202303
HOVERGENiHBG050582
InParanoidiP59998
KOiK05755
OMAiMLKHKIV
OrthoDBiEOG091G0OI8
PhylomeDBiP59998
TreeFamiTF105621
TF313087

Enzyme and pathway databases

ReactomeiR-HSA-2029482 Regulation of actin dynamics for phagocytic cup formation
R-HSA-3928662 EPHB-mediated forward signaling
R-HSA-5663213 RHO GTPases Activate WASPs and WAVEs
R-HSA-8856828 Clathrin-mediated endocytosis
SIGNORiP59998

Miscellaneous databases

ChiTaRSiARPC4 human
GeneWikiiARPC4
GenomeRNAii10093
PROiPR:P59998
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000241553 Expressed in 230 organ(s), highest expression level in vagina
CleanExiHS_ARPC4
ExpressionAtlasiP59998 baseline and differential
GenevisibleiP59998 HS

Family and domain databases

Gene3Di3.30.1460.20, 1 hit
InterProiView protein in InterPro
IPR034666 ARPC2/4
IPR008384 ARPC4
PANTHERiPTHR22629 PTHR22629, 1 hit
PfamiView protein in Pfam
PF05856 ARPC4, 1 hit
PIRSFiPIRSF039100 ARPC4, 1 hit
SUPFAMiSSF69645 SSF69645, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiARPC4_HUMAN
AccessioniPrimary (citable) accession number: P59998
Secondary accession number(s): C9JWM7
, E7ETI0, F6TTL5, O15509, Q6P0W5, Q96QJ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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