Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NADH-quinone oxidoreductase subunit G

Gene

nuoG

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).By similarity

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 3 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi49Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi60Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi63Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi77Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi111Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi115Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi118Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi124Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi164Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi167Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi170Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi214Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi240Iron-sulfur 4 (4Fe-4S)Sequence analysis1
Metal bindingi243Iron-sulfur 4 (4Fe-4S)Sequence analysis1
Metal bindingi247Iron-sulfur 4 (4Fe-4S)Sequence analysis1
Metal bindingi275Iron-sulfur 4 (4Fe-4S)Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciMBOV233413:G1GT4-3339-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit G (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I subunit G
NDH-1 subunit G
Gene namesi
Name:nuoG
Ordered Locus Names:BQ2027_MB3175
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001185581 – 806NADH-quinone oxidoreductase subunit GAdd BLAST806

Keywords - PTMi

Quinone

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 932Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST79
Domaini95 – 1344Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST40
Domaini233 – 2894Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated

Phylogenomic databases

HOGENOMiHOG000031440
OMAiGYGYVNR

Family and domain databases

CDDicd00207 fer2, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS
IPR010228 NADH_UbQ_OxRdtase_Gsu
IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd
PfamiView protein in Pfam
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
PF10588 NADH-G_4Fe-4S_3, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
SSF54292 SSF54292, 1 hit
TIGRFAMsiTIGR01973 NuoG, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS51839 4FE4S_HC3, 1 hit
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00641 COMPLEX1_75K_1, 1 hit
PS00642 COMPLEX1_75K_2, 1 hit
PS00643 COMPLEX1_75K_3, 1 hit

Sequencei

Sequence statusi: Complete.

P59962-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTQAADTDIR VGQPEMVTLT IDGVEISVPK GTLVIRAAEL MGIQIPRFCD
60 70 80 90 100
HPLLEPVGAC RQCLVEVEGQ RKPLASCTTV ATDDMVVRTQ LTSEIADKAQ
110 120 130 140 150
HGVMELLLIN HPLDCPMCDK GGECPLQNQA MSNGRTDSRF TEAKRTFAKP
160 170 180 190 200
INISAQVLLD RERCILCARC TRFSDQIAGD PFIDMQERGA LQQVGIYADE
210 220 230 240 250
PFESYFSGNT VQICPVGALT GTAYRFRARP FDLVSSPSVC EHCASGCAQR
260 270 280 290 300
TDHRRGKVLR RLAGDDPEVN EEWNCDKGRW AFTYATQPDV ITTPLIRDGG
310 320 330 340 350
DPKGALVPTS WSHAMAVAAQ GLAAARGRTG VLVGGRVTWE DAYAYAKFAR
360 370 380 390 400
ITLGTNDIDF RARPHSAEEA DFLAARIAGR HMAVSYADLE SAPVVLLVGF
410 420 430 440 450
EPEDESPIVF LRLRKAARRH RVPVYTIAPF ATGGLHKMSG RLIKTVPGGE
460 470 480 490 500
PAALDDLATG AVGDLLATPG AVIMVGERLA TVPGGLSAAA RLADTTGARL
510 520 530 540 550
AWVPRRAGER GALEAGALPT LLPGGRPLAD EVARAQVCAA WHIAELPAAA
560 570 580 590 600
GRDADGILAA AADETLAALL VGGIEPADFA DPDAVLAALD ATGFVVSLEL
610 620 630 640 650
RHSAVTERAD VVFPVAPTTQ KAGAFVNWEG RYRTFEPALR GSTLQAGQSD
660 670 680 690 700
HRVLDALADD MGVHLGVPTV EAAREELAAL GIWDGKHAAG PHIAATGPTQ
710 720 730 740 750
PEAGEAILTG WRMLLDEGRL QDGEPYLAGT ARTPVVRLSP DTAAEIGAAD
760 770 780 790 800
GEAVTVSTSR GSITLPCSVT DMPDRVVWLP LNSAGSTVHR QLRVTIGSIV

KIGAGS
Length:806
Mass (Da):85,412
Last modified:October 31, 2003 - v1
Checksum:iD674D74FA6FF3E81
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LT708304 Genomic DNA Translation: SIU01802.1
RefSeqiNP_856820.1, NC_002945.3
WP_003899937.1, NC_002945.4

Genome annotation databases

EnsemblBacteriaiCDO44446; CDO44446; Mb3175
PATRICifig|233413.5.peg.3493

Similar proteinsi

Entry informationi

Entry nameiNUOG_MYCBO
AccessioniPrimary (citable) accession number: P59962
Secondary accession number(s): A0A1R3Y385, X2BNJ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: October 31, 2003
Last modified: April 25, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again